FastQCFastQC Report
Fri 27 May 2016
SRR2140149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6621304
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC163650.055.608071
TATACAC196850.047.4613653
GGTATCA80600.047.418621
TTATACA199100.046.277792
GTATCAA154350.039.351181
TACACAT268700.035.067855
ATACACA291550.033.199954
ATCAACG181250.032.715663
TCAACGC183300.032.5104874
ACACATC284950.032.5067866
CAACGCA186850.031.8477695
TATCAAC192450.031.123262
AACGCAG193050.030.8249446
ACATCTC302800.030.74348
CACATCT313600.029.5101787
CATCTCC336500.027.464399
ACGCAGA218150.027.2011077
CGCAGAG222900.026.583698
GCAGAGT262000.022.6967439
GTGGTAT37800.022.0481171
Produced by FastQC (version 0.11.5)