##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140149_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6621304
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.738041026359763	31.0	25.0	32.0	18.0	33.0
2	29.404317487914767	31.0	28.0	33.0	18.0	33.0
3	29.774322097278723	31.0	29.0	33.0	25.0	33.0
4	30.807085281086625	33.0	31.0	33.0	28.0	33.0
5	31.83531083907339	33.0	32.0	33.0	30.0	33.0
6	34.756756071009576	37.0	34.0	38.0	29.0	38.0
7	36.191573442331	38.0	36.0	38.0	33.0	38.0
8	36.003104071343046	38.0	36.0	38.0	33.0	38.0
9	36.55986660633615	38.0	37.0	38.0	34.0	38.0
10-11	36.87876157022846	38.0	38.0	38.0	35.0	38.0
12-13	37.063724305665474	38.0	38.0	38.0	36.0	38.0
14-15	37.09437423504494	38.0	38.0	38.0	36.0	38.0
16-17	37.154268101872375	38.0	38.0	38.0	36.0	38.0
18-19	37.225285985962884	38.0	38.0	38.0	36.0	38.0
20-21	37.27402614606694	38.0	38.0	38.0	36.5	38.0
22-23	37.227861316737794	38.0	38.0	38.0	36.5	38.0
24-25	37.298125132503344	38.0	38.0	38.0	37.0	38.0
26-27	37.279717973486555	38.0	38.0	38.0	37.0	38.0
28-29	37.26423440400319	38.0	38.0	38.0	37.0	38.0
30-31	37.26034180042394	38.0	38.0	38.0	37.0	38.0
32-33	37.22475264227974	38.0	38.0	38.0	36.5	38.0
34-35	37.13440427612787	38.0	38.0	38.0	36.0	38.0
36-37	37.148399712265686	38.0	38.0	38.0	36.0	38.0
38-39	37.083819468276545	38.0	38.0	38.0	36.0	38.0
40-41	37.18575037457266	38.0	38.0	38.0	36.0	38.0
42-43	37.1725383759634	38.0	38.0	38.0	36.0	38.0
44-45	37.13340221881162	38.0	38.0	38.0	36.0	38.0
46-47	37.09308214075736	38.0	38.0	38.0	36.0	38.0
48-49	37.03862225706111	38.0	38.0	38.0	36.0	38.0
50-51	37.00215407300766	38.0	38.0	38.0	36.0	38.0
52-53	36.90997454168913	38.0	38.0	38.0	36.0	38.0
54-55	36.93230937875552	38.0	38.0	38.0	36.0	38.0
56-57	36.88802263835859	38.0	38.0	38.0	36.0	38.0
58-59	36.85402430223792	38.0	38.0	38.0	35.0	38.0
60-61	36.82993571283858	38.0	38.0	38.0	35.0	38.0
62-63	36.833263011481236	38.0	38.0	38.0	35.0	38.0
64-65	36.83770193364524	38.0	38.0	38.0	35.0	38.0
66-67	36.820744827988875	38.0	38.0	38.0	35.0	38.0
68-69	36.7917358523101	38.0	38.0	38.0	35.0	38.0
70-71	36.78871573001128	38.0	38.0	38.0	35.0	38.0
72-73	36.79272866194058	38.0	38.0	38.0	35.0	38.0
74-75	36.73566093137751	38.0	38.0	38.0	35.0	38.0
76-77	36.73692985380912	38.0	38.0	38.0	35.0	38.0
78-79	36.74145040165938	38.0	38.0	38.0	35.0	38.0
80-81	36.72443390004247	38.0	38.0	38.0	35.0	38.0
82-83	36.67110995788228	38.0	38.0	38.0	34.0	38.0
84-85	36.66448801570558	38.0	38.0	38.0	34.0	38.0
86-87	36.65780083584157	38.0	38.0	38.0	34.0	38.0
88-89	36.63783137517191	38.0	38.0	38.0	34.0	38.0
90-91	36.645841113394056	38.0	38.0	38.0	34.0	38.0
92-93	36.61731591524526	38.0	38.0	38.0	34.0	38.0
94-95	36.55401153564955	38.0	38.0	38.0	34.0	38.0
96-97	36.56984458454057	38.0	38.0	38.0	34.0	38.0
98-99	36.59957734176179	38.0	38.0	38.0	34.0	38.0
100	34.97157527452148	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	12.0
19	17.0
20	39.0
21	151.0
22	549.0
23	1699.0
24	4112.0
25	8067.0
26	14869.0
27	24013.0
28	35599.0
29	51145.0
30	68647.0
31	90257.0
32	119616.0
33	165158.0
34	254160.0
35	477309.0
36	1398679.0
37	3907204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.90197399183001	18.385623134053354	14.12865199966653	27.583750874450107
2	13.556636768570549	19.857306669812722	43.94202900302133	22.6440275585954
3	17.548235211674317	25.556174433314045	31.313786529058323	25.581803825953315
4	11.8511247935452	18.756441329381644	38.26207043204783	31.13036344502533
5	13.10504698168216	36.657341212546655	36.3192205039974	13.918391301773788
6	28.894142301878905	37.901325781145225	18.749645085016486	14.454886831959385
7	25.58237773103304	32.456401337259244	22.346232705823503	19.614988225884204
8	23.665398839865983	36.906853997339496	21.576686404974005	17.851060757820512
9	24.524157175082127	16.97387704899216	20.52586620399849	37.97609957192722
10-11	23.73845091540881	26.509294845849098	29.03280078969339	20.719453449048707
12-13	24.847990667699293	23.56569793502911	28.78726003216285	22.799051365108745
14-15	22.030532052296646	25.11628374108786	25.745721688658307	27.107462517957188
16-17	22.161608951952665	28.910521250798936	26.31045183848982	22.617417958758576
18-19	22.32333691369555	27.22922101145031	28.117354225089198	22.33008784976494
20-21	22.51227834674906	26.402172171275666	28.100087159308824	22.98546232266645
22-23	22.183785090046122	27.40863136312404	28.003504048724402	22.404079498105432
24-25	22.800150534926907	27.35053166425757	28.31429656604093	21.535021234774597
26-27	22.9482106500963	27.486911073857694	28.282066075113825	21.28281220093218
28-29	22.78963551714731	26.965935101452626	28.216608593340876	22.027820788059188
30-31	22.18728891729553	27.60058431324129	28.937380916065646	21.27474585339754
32-33	22.61674890206809	27.57374585798936	28.415688938669238	21.39381630127332
34-35	23.21526621394334	26.728502171444045	28.724770132736126	21.331461481876488
36-37	21.707833912515547	27.862191868364246	28.53395256752104	21.896021651599167
38-39	21.67562902680465	26.412478133653444	29.24419740655845	22.667695432983457
40-41	21.92913215720472	26.220384814861102	29.39594445778854	22.454538570145637
42-43	22.52542438031862	26.110321846578046	29.314043657898438	22.050210115204898
44-45	21.70671853045203	26.88931406601881	29.1794074406382	22.224559962890964
46-47	22.696296867346344	25.990851143749794	28.238734134326197	23.074117854577665
48-49	22.365635347922243	26.090731318387007	28.362471087232098	23.18116224645865
50-51	21.66921479446577	26.42055267250048	29.07756590310509	22.832666629928656
52-53	21.868239059854037	26.609215505815442	29.695382006629966	21.82716342770055
54-55	21.8084549458428	26.757871034705254	28.668413115871182	22.76526090358076
56-57	22.57408147111493	27.467888217141013	27.5828108197221	22.375219492021962
58-59	21.39346202403955	28.35478201547034	28.894683968709895	21.357071991780213
60-61	22.198157559267955	29.490592567051944	27.03054406692655	21.28070580675356
62-63	21.279864716650454	30.333144064593938	27.00784942504746	21.37914179370815
64-65	21.2831641436277	30.129044274227635	27.056639102788438	21.531152479356233
66-67	21.422425291779447	29.78174378448867	27.064478283511338	21.73135264022055
68-69	21.569868231969842	29.22157590371922	27.3731703769449	21.83538548736604
70-71	21.8300240997456	28.21680799166805	27.731957574214768	22.221210334371584
72-73	22.17709066039537	27.483007190320848	27.96502734410149	22.374874805182294
74-75	22.11503237749273	27.520279170270744	27.91039696612191	22.454291486114613
76-77	22.268685155753694	27.41237659295683	27.803917149369497	22.515021101919984
78-79	22.284038094891752	27.406358517919156	27.787680565673007	22.521922821516092
80-81	22.28182812428679	27.45982558479466	27.732224344579357	22.526121946339188
82-83	22.353146528548717	27.418730635185263	27.830966683815365	22.397156152450652
84-85	22.481386138420554	27.415594545335626	27.72907471999891	22.373944596244915
86-87	22.32751861097216	27.422417935531247	27.87389141196967	22.376172041526925
88-89	22.43182570260064	27.40397680870427	27.774824034286038	22.389373454409046
90-91	22.50112910078484	27.376911909605102	27.79797314456305	22.32398584504701
92-93	22.505253626709457	27.40577230882075	27.797386410388253	22.29158765408154
94-95	22.364383791159202	27.54470077460279	27.763293011779076	22.32762242245893
96-97	22.454199916948152	27.548053630454405	27.87658005829106	22.12116639430638
98-99	23.476277332002173	29.502422553033618	28.07821729785347	18.94308281711074
100	22.319264377334402	30.386246946167244	21.972883786148802	25.32160489034955
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1352.0
1	1215.0
2	1499.5
3	2110.0
4	2714.5
5	3842.5
6	4920.5
7	6249.0
8	7879.5
9	8599.5
10	8239.0
11	7985.5
12	8842.5
13	10896.5
14	15003.0
15	20036.5
16	24029.0
17	26470.5
18	26433.5
19	24468.5
20	22064.5
21	20122.5
22	20126.5
23	22948.0
24	28256.5
25	36192.0
26	46976.5
27	58781.0
28	71154.5
29	85476.5
30	99536.0
31	113056.0
32	127873.5
33	143526.5
34	158811.0
35	172541.0
36	187799.5
37	204180.0
38	216473.0
39	226232.0
40	237605.5
41	247664.5
42	261375.5
43	298472.0
44	348953.5
45	365571.0
46	345056.5
47	317375.5
48	291361.0
49	275070.0
50	268282.5
51	261074.5
52	250056.5
53	237092.0
54	223283.5
55	206698.5
56	189395.5
57	171794.5
58	153228.0
59	133479.0
60	111494.0
61	90438.0
62	71744.5
63	55774.0
64	42678.5
65	31724.5
66	23077.5
67	17493.0
68	13365.5
69	9628.0
70	6947.5
71	5082.5
72	3537.5
73	2340.5
74	1544.5
75	992.0
76	640.0
77	397.5
78	246.5
79	161.5
80	113.5
81	70.0
82	43.0
83	25.0
84	15.5
85	11.5
86	6.5
87	4.5
88	3.0
89	3.0
90	3.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.020736096696360715
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.945436914027983E-4
26-27	3.021354784549456E-4
28-29	0.0033777358243341615
30-31	0.005764722169895895
32-33	0.004421184983744751
34-35	0.0019397705061317667
36-37	0.0
38-39	7.689602135371754E-6
40-41	3.871580001090237E-4
42-43	0.0
44-45	7.859418888714323E-6
46-47	3.4897381078449627E-4
48-49	0.0013683015736668362
50-51	0.002565677925931783
52-53	0.0
54-55	0.0028405393259162788
56-57	0.03830385830541718
58-59	0.060746244246629756
60-61	0.0417981844049984
62-63	0.013714309737939714
64-65	2.9998844615927364E-4
66-67	0.0
68-69	8.836730187145155E-6
70-71	0.00259420896893705
72-73	0.004806735237854355
74-75	0.0038999115555772216
76-77	0.003093752513673917
78-79	0.00791655271628666
80-81	0.01584419568079189
82-83	0.04221245867804344
84-85	0.018290807044283663
86-87	0.0018037717162286621
88-89	0.0012646885609815894
90-91	9.058968449223374E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	55.0
20-21	244.0
22-23	467.0
24-25	681.0
26-27	1993.0
28-29	5099.0
30-31	15202.0
32-33	18937.0
34-35	25482.0
36-37	39785.0
38-39	45038.0
40-41	44022.0
42-43	48601.0
44-45	56997.0
46-47	56563.0
48-49	52862.0
50-51	46460.0
52-53	43525.0
54-55	46101.0
56-57	47143.0
58-59	51278.0
60-61	59440.0
62-63	64816.0
64-65	79137.0
66-67	90632.0
68-69	90452.0
70-71	133594.0
72-73	59408.0
74-75	50935.0
76-77	52811.0
78-79	54023.0
80-81	54020.0
82-83	50482.0
84-85	49614.0
86-87	51234.0
88-89	53029.0
90-91	54232.0
92-93	54005.0
94-95	63476.0
96-97	198099.0
98-99	437886.0
100-101	4173444.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.95584009539596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.13887974291694	77.47963133560295
2	6.9750622332994245	11.990946679618414
3	1.6834715438366388	4.341126324815145
4	0.5683723626228933	1.9541969566502326
5	0.26010131405533043	1.1178613479771176
6	0.1355178668933657	0.6989131258053177
7	0.06802906457399505	0.40932467774531783
8	0.048639335336428294	0.3344667944419498
9	0.026717358716457078	0.20668617122428062
>10	0.09269280765095993	1.2186097721227918
>50	0.001819955265279207	0.10444820722862931
>100	6.155110406380331E-4	0.09156293865559388
>500	5.393590181965113E-5	0.02793252895859617
>1k	2.6967889867545788E-5	0.024293139153649355
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5102765255907296E-5
2	0.0	1.5102765255907296E-5	0.0	1.5102765255907296E-5	1.5102765255907296E-5
3	0.0	1.5102765255907296E-5	0.0	1.5102765255907296E-5	1.5102765255907296E-5
4	0.0	6.0411061023629185E-5	0.0	3.0205530511814593E-5	3.0205530511814593E-5
5	0.0	7.551382627953647E-5	0.0	4.5308295767721885E-5	3.0205530511814593E-5
6	0.0	7.551382627953647E-5	0.0	4.5308295767721885E-5	3.0205530511814593E-5
7	0.0	7.551382627953647E-5	0.0	4.5308295767721885E-5	3.0205530511814593E-5
8	0.0	7.551382627953647E-5	0.0	4.5308295767721885E-5	4.5308295767721885E-5
9	0.0	2.1143871358270214E-4	0.0	6.0411061023629185E-5	4.5308295767721885E-5
10-11	0.0	2.1143871358270214E-4	0.0	1.5102765255907294E-4	4.5308295767721885E-5
12-13	0.0	2.1143871358270214E-4	0.0	2.1143871358270214E-4	4.5308295767721885E-5
14-15	0.0	2.6429839197837765E-4	0.0	2.1143871358270214E-4	5.2859678395675535E-5
16-17	0.0	2.7184977460633134E-4	0.0	2.4164424409451674E-4	6.0411061023629185E-5
18-19	0.0	2.945039224901923E-4	0.0	2.9450392249019225E-4	6.0411061023629185E-5
20-21	0.0	3.171580703740532E-4	0.0	3.322608356299605E-4	6.0411061023629185E-5
22-23	0.0	4.153260445374506E-4	0.0	3.398122182579141E-4	6.0411061023629185E-5
24-25	0.0	6.343161407481063E-4	0.0	4.0777466190949696E-4	6.0411061023629185E-5
26-27	0.0	6.64521671259921E-4	0.0	5.285967839567553E-4	7.551382627953647E-5
28-29	0.0	6.64521671259921E-4	0.0	0.0011251560115650935	7.551382627953647E-5
30-31	0.0	7.551382627953648E-4	0.0	0.0030885154948330417	7.551382627953647E-5
32-33	1.5102765255907296E-5	7.551382627953648E-4	0.0	0.0074683174190461575	1.0571935679135107E-4
34-35	1.5102765255907296E-5	8.38203471702855E-4	0.0	0.012739182493357804	1.1327073941930472E-4
36-37	1.5102765255907296E-5	9.43922828494206E-4	0.0	0.021023049236222956	1.2082212204725837E-4
38-39	1.5102765255907296E-5	9.66576976378067E-4	0.0	0.042967367153056255	1.2082212204725837E-4
40-41	1.5102765255907296E-5	0.001026988037401696	0.0	0.0660292896988267	1.2082212204725837E-4
42-43	1.5102765255907296E-5	0.0010420908026576034	0.0	0.07967463810753894	1.2082212204725837E-4
44-45	1.5102765255907296E-5	0.0011629129247048616	0.0	0.09575908310508022	1.2082212204725837E-4
46-47	1.5102765255907296E-5	0.0012610808988682593	0.0	0.11316502006251336	1.434762699311193E-4
48-49	1.5102765255907296E-5	0.0012686322814962128	0.0	0.12914374570326329	1.5102765255907294E-4
50-51	1.5102765255907296E-5	0.0014649682298230077	0.0	0.14471469668210368	1.5102765255907294E-4
52-53	1.5102765255907296E-5	0.0014649682298230077	0.0	0.18489560364544508	1.8123318307088754E-4
54-55	1.5102765255907296E-5	0.0014725196124509613	0.0	0.23542945619171088	2.1143871358270214E-4
56-57	1.5102765255907296E-5	0.0014951737603348223	0.0	0.30050727167941543	2.1143871358270214E-4
58-59	1.5102765255907296E-5	0.0015102765255907296	0.0	0.3430744155531901	2.1143871358270214E-4
60-61	1.5102765255907296E-5	0.0015102765255907296	0.0	0.3621038997756333	2.1143871358270214E-4
62-63	1.5102765255907296E-5	0.0015178279082186832	0.0	0.37844509178252506	2.1143871358270214E-4
64-65	1.5102765255907296E-5	0.0016008931171261733	0.0	0.3846447769200749	2.1143871358270214E-4
66-67	1.5102765255907296E-5	0.0016159958823820807	0.0	0.3906934344050658	2.1143871358270214E-4
68-69	1.5102765255907296E-5	0.0016310986476379878	0.0	0.3966816808290331	2.1143871358270214E-4
70-71	1.5102765255907296E-5	0.0016386500302659414	0.0	0.40155232262406315	2.1143871358270214E-4
72-73	1.5102765255907296E-5	0.0016613041781498026	0.0	0.40566782615629793	2.1143871358270214E-4
74-75	1.5102765255907296E-5	0.00167640694340571	0.0	0.40868837920747936	2.1143871358270214E-4
76-77	1.5102765255907296E-5	0.0017896776828250146	0.0	0.41185995991121993	2.1143871358270214E-4
78-79	1.5102765255907296E-5	0.001827434595964783	0.0	0.41385352492499966	2.1143871358270214E-4
80-81	1.5102765255907296E-5	0.00184253736122069	0.0	0.41463131733567893	2.1143871358270214E-4
82-83	1.5102765255907296E-5	0.0018500887438486439	0.0	0.41520522241540336	2.1143871358270214E-4
84-85	1.5102765255907296E-5	0.0018953970396163656	0.0	0.41543176389424197	2.1143871358270214E-4
86-87	1.5102765255907296E-5	0.0019558081006399945	0.0	0.41556768878154515	2.1143871358270214E-4
88	1.5102765255907296E-5	0.002114387135827021	0.0	0.41556768878154515	2.1143871358270214E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	16365	0.0	55.60807	1
TATACAC	19685	0.0	47.461365	3
GGTATCA	8060	0.0	47.41862	1
TTATACA	19910	0.0	46.27779	2
GTATCAA	15435	0.0	39.35118	1
TACACAT	26870	0.0	35.06785	5
ATACACA	29155	0.0	33.19995	4
ATCAACG	18125	0.0	32.71566	3
TCAACGC	18330	0.0	32.510487	4
ACACATC	28495	0.0	32.506786	6
CAACGCA	18685	0.0	31.847769	5
TATCAAC	19245	0.0	31.12326	2
AACGCAG	19305	0.0	30.824944	6
ACATCTC	30280	0.0	30.7434	8
CACATCT	31360	0.0	29.510178	7
CATCTCC	33650	0.0	27.46439	9
ACGCAGA	21815	0.0	27.201107	7
CGCAGAG	22290	0.0	26.58369	8
GCAGAGT	26200	0.0	22.696743	9
GTGGTAT	3780	0.0	22.048117	1
>>END_MODULE