FastQCFastQC Report
Fri 27 May 2016
SRR2140148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6347636
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC124500.052.240931
GGTATCA81550.050.6823231
TATACAC154600.043.917593
TTATACA157350.042.167182
GTATCAA153100.037.4581451
TACACAT205700.032.9048845
ACACATC208600.031.6575136
ATCAACG179250.031.5140613
TCAACGC181800.031.1882324
ACATCTC214350.030.808298
CAACGCA184950.030.6342035
ATACACA232150.030.1568494
TATCAAC188950.030.0827122
AACGCAG190750.029.7691786
CACATCT228900.028.6653867
CATCTCC243950.026.8622959
ACGCAGA215900.026.2622537
CGCAGAG220200.025.7302278
TGGTATC37750.022.949782
GTGGTAT40750.022.8158511
Produced by FastQC (version 0.11.5)