##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140148_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6347636
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.85642135119279	31.0	25.0	32.0	18.0	33.0
2	29.534269135785355	31.0	28.0	33.0	18.0	33.0
3	29.890196602325652	31.0	29.0	33.0	25.0	33.0
4	30.897720348173713	33.0	31.0	33.0	28.0	33.0
5	31.896885234124955	33.0	32.0	33.0	30.0	33.0
6	34.86291904576759	37.0	34.0	38.0	29.0	38.0
7	36.27714427859443	38.0	37.0	38.0	33.0	38.0
8	36.09561874688467	38.0	36.0	38.0	33.0	38.0
9	36.629972008476855	38.0	37.0	38.0	34.0	38.0
10-11	36.936220192840295	38.0	38.0	38.0	35.0	38.0
12-13	37.1099155654168	38.0	38.0	38.0	36.0	38.0
14-15	37.139510677675915	38.0	38.0	38.0	36.0	38.0
16-17	37.201735654029314	38.0	38.0	38.0	36.0	38.0
18-19	37.25866164978584	38.0	38.0	38.0	36.0	38.0
20-21	37.302960821376004	38.0	38.0	38.0	37.0	38.0
22-23	37.252943852377	38.0	38.0	38.0	36.5	38.0
24-25	37.32631518031883	38.0	38.0	38.0	37.0	38.0
26-27	37.30539601434013	38.0	38.0	38.0	37.0	38.0
28-29	37.290046096548025	38.0	38.0	38.0	37.0	38.0
30-31	37.29026357742896	38.0	38.0	38.0	37.0	38.0
32-33	37.26679716662441	38.0	38.0	38.0	37.0	38.0
34-35	37.1784649389213	38.0	38.0	38.0	36.0	38.0
36-37	37.1967541509793	38.0	38.0	38.0	36.0	38.0
38-39	37.14205185605171	38.0	38.0	38.0	36.0	38.0
40-41	37.214183636075774	38.0	38.0	38.0	37.0	38.0
42-43	37.197361381206036	38.0	38.0	38.0	37.0	38.0
44-45	37.15809315756786	38.0	38.0	38.0	36.0	38.0
46-47	37.119494313321475	38.0	38.0	38.0	36.0	38.0
48-49	37.06315920118843	38.0	38.0	38.0	36.0	38.0
50-51	37.02934015558577	38.0	38.0	38.0	36.0	38.0
52-53	36.93869991012719	38.0	38.0	38.0	36.0	38.0
54-55	36.9688252579754	38.0	38.0	38.0	36.0	38.0
56-57	36.92661580934984	38.0	38.0	38.0	36.0	38.0
58-59	36.898330373503285	38.0	38.0	38.0	36.0	38.0
60-61	36.88221333670384	38.0	38.0	38.0	35.5	38.0
62-63	36.88632621627197	38.0	38.0	38.0	35.0	38.0
64-65	36.893505503623786	38.0	38.0	38.0	35.0	38.0
66-67	36.88215563637615	38.0	38.0	38.0	35.0	38.0
68-69	36.85560692443316	38.0	38.0	38.0	35.0	38.0
70-71	36.851666525971496	38.0	38.0	38.0	35.0	38.0
72-73	36.85481930273875	38.0	38.0	38.0	35.0	38.0
74-75	36.79618324796713	38.0	38.0	38.0	35.0	38.0
76-77	36.801815376923756	38.0	38.0	38.0	35.0	38.0
78-79	36.809311740866775	38.0	38.0	38.0	35.0	38.0
80-81	36.79538980141064	38.0	38.0	38.0	35.0	38.0
82-83	36.742662484717115	38.0	38.0	38.0	35.0	38.0
84-85	36.73838086994746	38.0	38.0	38.0	35.0	38.0
86-87	36.730482732628616	38.0	38.0	38.0	35.0	38.0
88-89	36.71129993526609	38.0	38.0	38.0	34.5	38.0
90-91	36.71798625362746	38.0	38.0	38.0	34.5	38.0
92-93	36.69054538904946	38.0	38.0	38.0	34.0	38.0
94-95	36.62420121393916	38.0	38.0	38.0	34.0	38.0
96-97	36.645432946855735	38.0	38.0	38.0	34.0	38.0
98-99	36.67148388430532	38.0	38.0	38.0	34.0	38.0
100	35.13405655629812	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	13.0
19	15.0
20	31.0
21	123.0
22	461.0
23	1413.0
24	3598.0
25	7331.0
26	13126.0
27	21448.0
28	32700.0
29	46789.0
30	63266.0
31	83117.0
32	109698.0
33	151974.0
34	232257.0
35	432778.0
36	1260047.0
37	3887450.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.46207123407832	18.157499894448893	14.332406584120449	27.04802228735233
2	13.685291783766953	19.916639512215724	43.748136742461725	22.6499319615556
3	17.82668382371012	25.298693875956342	31.047416707574282	25.827205592759256
4	12.00736778227359	18.390657561334645	38.66070770283614	30.941266953555623
5	13.273760499184265	36.559358476131905	36.17570383682997	13.991177187853873
6	29.25613567003527	37.894375165809755	18.73946458177501	14.11002458237996
7	25.925982523257478	32.48557415705626	22.52203812568963	19.06640519399663
8	23.80314498184836	36.851356946113484	21.482375485928934	17.863122586109224
9	24.782769522385973	17.033963510194976	20.801696883690244	37.38157008372881
10-11	23.821348609151503	26.567607216292803	29.042843666524043	20.56820050803165
12-13	24.95114716722887	23.73451313213297	28.94071903303844	22.373620667599717
14-15	22.062646629390848	25.253165115327974	26.140290653087227	26.54389760219395
16-17	21.994211703380596	28.94818637993735	26.69664738179694	22.360954534885114
18-19	22.035565681459996	27.44851784191784	28.581333271157956	21.934583205464207
20-21	22.45273679338351	26.650490316087904	28.52880912252908	22.3679637679995
22-23	22.13909096645343	27.297585081527792	28.363450196831145	22.199873755187628
24-25	22.323037763587745	27.51545525069645	28.51909820795432	21.64240877776149
26-27	22.214004230335423	27.86487036964811	28.463215819201025	21.45790958081544
28-29	21.906620625876645	27.54213219432669	28.566604409756724	21.98464277003994
30-31	22.76201837442802	27.193258532309137	28.507566623361473	21.537156469901365
32-33	22.179275840255386	27.35216412032556	28.730660186053935	21.737899853365114
34-35	22.16096989038369	26.816588763729015	28.951743339056847	22.07069800683045
36-37	21.740175294975014	27.604050216230284	28.787097672789407	21.868676816005294
38-39	21.700119868147443	26.6283132429165	29.33311245114044	22.338454437795615
40-41	21.953637558849188	26.480383739278174	29.35949399892706	22.206484702945577
42-43	22.335756966088216	26.39061659740299	29.299870012738623	21.973756423770176
44-45	21.787499746037696	27.022932632157065	29.18672422708621	22.002843394719026
46-47	22.50465034113524	26.361734458618617	28.390665358790084	22.74294984145606
48-49	22.260357755004893	26.461488825546535	28.504100362179123	22.774053057269445
50-51	21.750013561856573	26.759565734634204	28.955350162931516	22.535070540577706
52-53	21.92169617314042	26.865267123089794	29.354992604142478	21.858044099627314
54-55	21.84463339293286	26.91239944659735	28.685417833419933	22.557549327049852
56-57	22.432226275272352	27.424278090402467	27.862957003428946	22.28053863089624
58-59	21.566896385710166	28.044812122866535	28.851172964964277	21.53711852645902
60-61	22.149820152804732	28.789703554548197	27.572036186184594	21.488440106462473
62-63	21.425037896563303	29.432785638394225	27.57092827777466	21.571248187267813
64-65	21.45635454875355	29.25003327161556	27.620192095555545	21.673420084075346
66-67	21.63284582329668	29.05290690581872	27.506254786734512	21.807992484150088
68-69	21.715113880272614	28.659046776348735	27.78072661397551	21.84511272940314
70-71	21.860019745740367	27.943763581237512	28.046005363116322	22.1502113099058
72-73	22.10362566250256	27.437161823402175	28.164760021363072	22.294452492732198
74-75	22.037231783911924	27.534764226450136	28.103005058836466	22.32499893080147
76-77	22.212385244404782	27.444589905795976	27.94880381461018	22.394221035189066
78-79	22.217838655813594	27.496505963369998	27.877876240920813	22.407779139895602
80-81	22.232260890766927	27.5322057830009	27.862675341886007	22.37285798434617
82-83	22.280097739969424	27.456635019209003	27.940922316362144	22.32234492445943
84-85	22.425295948443065	27.445672265986364	27.87040419258246	22.258627592988113
86-87	22.193893597252444	27.52303567613909	27.932015216627885	22.351055509980583
88-89	22.338212453908113	27.52968569674785	27.8578504085841	22.274251440759933
90-91	22.4105274445443	27.520020118612535	27.8522005818225	22.217251855020663
92-93	22.452295847594247	27.49470592674872	27.827267179421888	22.22573104623515
94-95	22.275096737530355	27.620496707551922	27.838464021756987	22.265942533160736
96-97	22.330078633208508	27.650145742538445	27.98521988206791	22.034555742185137
98-99	23.380855014033326	29.578092147943817	28.19427870346943	18.846774134553428
100	29.730365037447935	40.66496515842222	29.604669804129845	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1331.0
1	1198.5
2	1523.5
3	2145.0
4	2703.5
5	3745.5
6	4753.0
7	6153.0
8	7905.5
9	8641.0
10	8288.5
11	8179.5
12	9155.0
13	11190.5
14	15256.0
15	20280.5
16	24230.5
17	26313.0
18	25969.0
19	23873.0
20	21560.0
21	19926.0
22	20063.5
23	22805.0
24	28224.0
25	36409.5
26	47492.5
27	60232.0
28	72820.0
29	86393.5
30	99826.0
31	113234.5
32	127947.5
33	143396.0
34	158253.0
35	172200.5
36	187644.0
37	201880.0
38	212269.5
39	221414.0
40	232796.0
41	243637.5
42	254249.0
43	281687.0
44	315782.5
45	324069.5
46	310893.0
47	293477.0
48	275945.0
49	264140.5
50	256731.0
51	247813.0
52	236285.0
53	223428.0
54	209603.5
55	193811.5
56	176716.5
57	159415.5
58	142078.5
59	122492.0
60	102320.5
61	83207.0
62	65182.5
63	50191.5
64	38162.5
65	28295.0
66	20881.0
67	15880.5
68	12002.0
69	8771.5
70	6437.5
71	4653.5
72	3286.5
73	2215.0
74	1494.5
75	1015.5
76	667.5
77	408.0
78	269.5
79	187.0
80	117.5
81	73.5
82	57.0
83	45.5
84	31.0
85	20.5
86	12.0
87	11.5
88	9.0
89	5.0
90	3.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.019282769207308044
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.9390831815407627E-4
26-27	2.757707793282224E-4
28-29	0.003357882131716001
30-31	0.0054924233755644924
32-33	0.0037810230768178118
34-35	0.0018485638998954252
36-37	0.0
38-39	0.0
40-41	4.440285825235194E-4
42-43	8.128267309450046E-6
44-45	1.638466205569507E-5
46-47	1.9839652620909045E-4
48-49	0.0015013763868026012
50-51	0.002506252384829503
52-53	0.0
54-55	0.002986571606083493
56-57	0.03795695140083074
58-59	0.05990136034672072
60-61	0.04182715783850468
62-63	0.013204233121794928
64-65	3.8330180453141177E-4
66-67	0.0
68-69	0.0
70-71	0.002667267405917951
72-73	0.004812038377565955
74-75	0.004114335768173694
76-77	0.002987302518826029
78-79	0.007085885014384931
80-81	0.015616181786516763
82-83	0.041335655256065494
84-85	0.018616678477625128
86-87	0.001843448106885147
88-89	0.0011664486396804339
90-91	7.238393597785631E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	57.0
20-21	258.0
22-23	554.0
24-25	646.0
26-27	1858.0
28-29	4828.0
30-31	14440.0
32-33	17542.0
34-35	23898.0
36-37	37360.0
38-39	42549.0
40-41	41455.0
42-43	45923.0
44-45	53634.0
46-47	55241.0
48-49	50602.0
50-51	44963.0
52-53	41432.0
54-55	43984.0
56-57	44938.0
58-59	48785.0
60-61	54306.0
62-63	54701.0
64-65	65493.0
66-67	73530.0
68-69	74943.0
70-71	106660.0
72-73	53314.0
74-75	48609.0
76-77	51035.0
78-79	52525.0
80-81	51963.0
82-83	48845.0
84-85	48202.0
86-87	49233.0
88-89	50872.0
90-91	52478.0
92-93	51881.0
94-95	60975.0
96-97	190692.0
98-99	1442197.0
100-101	3050235.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.57813693953157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.26213524890073	77.24465370784948
2	6.8166962114170255	11.667203237116645
3	1.596801551011752	4.0995390539322045
4	0.6012661141850839	2.058209354273246
5	0.2800278272416784	1.1982129873283924
6	0.15006233246522532	0.7705232902304741
7	0.08212863872409623	0.4919891123971616
8	0.05113395960041078	0.3500759197555543
9	0.039481700420998335	0.3040893328710381
>10	0.11803406291923646	1.5558707738373307
>50	0.0014565835462406283	0.08085792200718545
>100	6.883700676470667E-4	0.10400538670802845
>500	5.826634215788376E-5	0.037571954173778684
>1k	2.9133157732331777E-5	0.03719796751952505
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5753896411199382E-5
2	0.0	0.0	0.0	0.0	1.5753896411199382E-5
3	0.0	1.5753896411199382E-5	0.0	1.5753896411199382E-5	1.5753896411199382E-5
4	0.0	3.1507792822398764E-5	0.0	1.5753896411199382E-5	1.5753896411199382E-5
5	0.0	3.1507792822398764E-5	0.0	1.5753896411199382E-5	1.5753896411199382E-5
6	0.0	3.1507792822398764E-5	0.0	1.5753896411199382E-5	3.1507792822398764E-5
7	0.0	4.7261689233598146E-5	0.0	1.5753896411199382E-5	3.1507792822398764E-5
8	0.0	4.7261689233598146E-5	0.0	1.5753896411199382E-5	3.1507792822398764E-5
9	0.0	7.87694820559969E-5	0.0	1.5753896411199382E-5	3.1507792822398764E-5
10-11	0.0	7.87694820559969E-5	0.0	7.089253385039721E-5	3.1507792822398764E-5
12-13	0.0	7.87694820559969E-5	0.0	7.87694820559969E-5	3.1507792822398764E-5
14-15	0.0	9.452337846719629E-5	0.0	7.87694820559969E-5	3.1507792822398764E-5
16-17	0.0	1.1027727487839567E-4	0.0	8.66464302615966E-5	3.1507792822398764E-5
18-19	0.0	1.4966201590639413E-4	0.0	9.452337846719629E-5	3.1507792822398764E-5
20-21	0.0	1.732928605231932E-4	0.0	1.1027727487839567E-4	3.1507792822398764E-5
22-23	0.0	1.732928605231932E-4	0.0	1.1815422308399536E-4	4.7261689233598146E-5
24-25	0.0	1.8116980872879288E-4	0.0	1.4966201590639413E-4	7.87694820559969E-5
26-27	0.0	1.8904675693439259E-4	0.0	2.441853943735904E-4	7.87694820559969E-5
28-29	0.0	1.8904675693439259E-4	0.0	5.986480636255765E-4	7.87694820559969E-5
30-31	0.0	2.2055454975679134E-4	0.0	0.002260684135007111	7.87694820559969E-5
32-33	0.0	2.2055454975679134E-4	0.0	0.006073127066517362	9.452337846719629E-5
34-35	0.0	2.8357013540158884E-4	0.0	0.010838680730905175	9.452337846719629E-5
36-37	0.0	3.5446266925198613E-4	0.0	0.01808547308005689	9.452337846719629E-5
38-39	0.0	3.7809351386878517E-4	0.0	0.03723433416786974	9.452337846719629E-5
40-41	0.0	4.0960130669118395E-4	0.0	0.05767501476140094	9.452337846719629E-5
42-43	0.0	4.253552031023833E-4	0.0	0.06947468317338927	9.452337846719629E-5
44-45	0.0	5.1200163336398E-4	0.0	0.08318057305113274	1.0240032667279599E-4
46-47	0.0	5.907711154199769E-4	0.0	0.09812314379715534	1.1815422308399536E-4
48-49	0.0	6.065250118311761E-4	0.0	0.11175026419284281	1.2603117128959506E-4
50-51	0.0	7.168022867095719E-4	0.0	0.12607843297882865	1.2603117128959506E-4
52-53	0.0	7.246792349151716E-4	0.0	0.16487240289140714	1.5753896411199383E-4
54-55	0.0	7.561870277375703E-4	0.0	0.21226012329629487	1.8904675693439259E-4
56-57	0.0	8.034487169711684E-4	0.0	0.275519894335466	1.8904675693439259E-4
58-59	0.0	8.507104062047666E-4	0.0	0.3172440889805276	1.8904675693439259E-4
60-61	0.0	8.979720954383648E-4	0.0	0.3333288172163621	1.8904675693439259E-4
62-63	0.0	8.979720954383648E-4	0.0	0.3449158080267993	1.8904675693439259E-4
64-65	0.0	9.92495473905561E-4	0.0	0.35022487111737344	1.8904675693439259E-4
66-67	0.0	0.0010082493703167605	0.0	0.3553685182956301	1.8904675693439259E-4
68-69	0.0	0.0010555110595503586	0.0	0.36063819664517627	1.8904675693439259E-4
70-71	0.0	0.0010870188523727574	0.0	0.3653092269310969	1.8904675693439259E-4
72-73	0.0	0.0011027727487839568	0.0	0.3690665312251679	2.2055454975679134E-4
74-75	0.0	0.0011185266451951563	0.0	0.3720440176468846	2.2055454975679134E-4
76-77	0.0	0.0011421574898119553	0.0	0.3749269806901341	2.2055454975679134E-4
78-79	0.0	0.0011500344380175548	0.0	0.3765732628651044	2.2055454975679134E-4
80-81	0.0	0.0011500344380175548	0.0	0.3772034187215524	2.2843149796239102E-4
82-83	0.0	0.0011579113862231546	0.0	0.377802066785178	2.520623425791901E-4
84-85	0.0	0.0011815422308399538	0.0	0.3780068674385235	2.520623425791901E-4
86-87	0.0	0.001197296127251153	0.0	0.37812502166160755	2.599392907847898E-4
88	0.0	0.0012130500236623525	0.0	0.37812502166160755	2.678162389903895E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	12450	0.0	52.24093	1
GGTATCA	8155	0.0	50.682323	1
TATACAC	15460	0.0	43.91759	3
TTATACA	15735	0.0	42.16718	2
GTATCAA	15310	0.0	37.458145	1
TACACAT	20570	0.0	32.904884	5
ACACATC	20860	0.0	31.657513	6
ATCAACG	17925	0.0	31.514061	3
TCAACGC	18180	0.0	31.188232	4
ACATCTC	21435	0.0	30.80829	8
CAACGCA	18495	0.0	30.634203	5
ATACACA	23215	0.0	30.156849	4
TATCAAC	18895	0.0	30.082712	2
AACGCAG	19075	0.0	29.769178	6
CACATCT	22890	0.0	28.665386	7
CATCTCC	24395	0.0	26.862295	9
ACGCAGA	21590	0.0	26.262253	7
CGCAGAG	22020	0.0	25.730227	8
TGGTATC	3775	0.0	22.94978	2
GTGGTAT	4075	0.0	22.815851	1
>>END_MODULE