FastQCFastQC Report
Fri 27 May 2016
SRR2140147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6572555
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78700.051.0564081
CTTATAC143550.043.27261
GTATCAA148950.038.484861
TATACAC173600.037.1923873
TTATACA179100.035.251892
TCAACGC179650.031.429684
ATCAACG179500.031.2432443
CAACGCA184400.030.6200775
TATCAAC186500.030.1902772
AACGCAG189000.029.8523836
TACACAT224450.029.0309165
ACACATC223400.028.0659926
ACATCTC236000.026.4776788
ATACACA256000.026.2153474
ACGCAGA218900.025.832927
CACATCT245150.025.4202027
CGCAGAG222150.025.41688
CATCTCC268750.023.235319
GTGGTAT40500.022.105611
TGGTATC37100.021.8441182
Produced by FastQC (version 0.11.5)