##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140147_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6572555
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.11694112867827	31.0	25.0	32.0	18.0	33.0
2	29.592867766036193	31.0	29.0	33.0	18.0	33.0
3	29.954296160321213	31.0	29.0	33.0	25.0	33.0
4	30.91028983401432	33.0	31.0	33.0	28.0	33.0
5	31.900046176867292	33.0	32.0	33.0	30.0	33.0
6	34.87619898197885	37.0	34.0	38.0	29.0	38.0
7	36.285921380650294	38.0	37.0	38.0	33.0	38.0
8	36.105315817060486	38.0	36.0	38.0	33.0	38.0
9	36.62848222647053	38.0	37.0	38.0	34.0	38.0
10-11	36.926953140749674	38.0	38.0	38.0	35.0	38.0
12-13	37.099604872077904	38.0	38.0	38.0	36.0	38.0
14-15	37.12339447901159	38.0	38.0	38.0	36.0	38.0
16-17	37.18329888452816	38.0	38.0	38.0	36.0	38.0
18-19	37.23701262294496	38.0	38.0	38.0	36.0	38.0
20-21	37.27949475231329	38.0	38.0	38.0	37.0	38.0
22-23	37.22920220618696	38.0	38.0	38.0	36.5	38.0
24-25	37.30209192922699	38.0	38.0	38.0	37.0	38.0
26-27	37.27990837145411	38.0	38.0	38.0	37.0	38.0
28-29	37.24733853653904	38.0	38.0	38.0	37.0	38.0
30-31	37.24620039038336	38.0	38.0	38.0	37.0	38.0
32-33	37.23328126749155	38.0	38.0	38.0	37.0	38.0
34-35	37.14255986531984	38.0	38.0	38.0	36.0	38.0
36-37	37.16096705491775	38.0	38.0	38.0	36.0	38.0
38-39	37.095545152365176	38.0	38.0	38.0	36.0	38.0
40-41	37.17900174031848	38.0	38.0	38.0	36.0	38.0
42-43	37.163919999582234	38.0	38.0	38.0	36.0	38.0
44-45	37.1220893051876	38.0	38.0	38.0	36.0	38.0
46-47	37.08543718825224	38.0	38.0	38.0	36.0	38.0
48-49	37.03033423640035	38.0	38.0	38.0	36.0	38.0
50-51	36.99512083581986	38.0	38.0	38.0	36.0	38.0
52-53	36.90276209624522	38.0	38.0	38.0	36.0	38.0
54-55	36.92989380707765	38.0	38.0	38.0	36.0	38.0
56-57	36.88878077532215	38.0	38.0	38.0	36.0	38.0
58-59	36.86109685771458	38.0	38.0	38.0	35.5	38.0
60-61	36.845272192307746	38.0	38.0	38.0	35.0	38.0
62-63	36.853258174523404	38.0	38.0	38.0	35.0	38.0
64-65	36.861052125088754	38.0	38.0	38.0	35.0	38.0
66-67	36.85278977163352	38.0	38.0	38.0	35.0	38.0
68-69	36.83513113092944	38.0	38.0	38.0	35.0	38.0
70-71	36.834310207172166	38.0	38.0	38.0	35.0	38.0
72-73	36.85002204320648	38.0	38.0	38.0	35.0	38.0
74-75	36.792851220005375	38.0	38.0	38.0	35.0	38.0
76-77	36.7948082569431	38.0	38.0	38.0	35.0	38.0
78-79	36.79597425988447	38.0	38.0	38.0	35.0	38.0
80-81	36.775735993435944	38.0	38.0	38.0	35.0	38.0
82-83	36.720692610755165	38.0	38.0	38.0	35.0	38.0
84-85	36.715420068099725	38.0	38.0	38.0	35.0	38.0
86-87	36.70932036245921	38.0	38.0	38.0	34.5	38.0
88-89	36.68293421095656	38.0	38.0	38.0	34.0	38.0
90-91	36.6935875568589	38.0	38.0	38.0	34.0	38.0
92-93	36.66640395544211	38.0	38.0	38.0	34.0	38.0
94-95	36.59221531208553	38.0	38.0	38.0	34.0	38.0
96-97	36.61230284410692	38.0	38.0	38.0	34.0	38.0
98-99	36.63908029663388	38.0	38.0	38.0	34.0	38.0
100	35.045328901602254	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	11.0
19	13.0
20	34.0
21	119.0
22	514.0
23	1694.0
24	3993.0
25	8232.0
26	14570.0
27	23535.0
28	35855.0
29	50683.0
30	68562.0
31	89844.0
32	117773.0
33	161992.0
34	247256.0
35	454910.0
36	1296169.0
37	3996795.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.38186063106357	18.37912349154933	14.433154229976013	26.80586164741109
2	13.593615629964392	19.99428412552827	43.718785063184306	22.69331518132303
3	17.718421527092584	25.337056897964338	31.232055114031	25.712466460912083
4	11.919702459697941	18.324411130831162	38.78147843570727	30.97440797376363
5	13.174480852575595	36.657707695104875	36.213892466476125	13.953918985843405
6	29.271295561619493	38.03397613256945	18.648349081901937	14.046379223909119
7	25.73388887578727	32.810954035378934	22.520481000158995	18.934676088674802
8	23.93734247944673	36.93551442323419	21.33193255895158	17.7952105383675
9	24.69805121448204	17.091268159794783	20.82745598933748	37.3832246363857
10-11	23.69603601643501	26.655790632410074	29.022389314353397	20.625784036801516
12-13	24.777190909775573	23.90149645001069	28.93282749250482	22.38848514770892
14-15	21.980865888531934	25.494560334603516	26.17696618742635	26.347607589438205
16-17	21.87920831396618	29.182433619802346	26.75099713886	22.187360927371472
18-19	21.903719329849654	27.52838888377503	28.64894245845033	21.918949327924985
20-21	22.35534246106387	26.776638132206916	28.586728338122885	22.281291068606325
22-23	22.22901606847345	27.27112269944998	28.420165231209882	22.07969600086669
24-25	22.3718072405026	27.163845329317667	28.716658743670482	21.74768868650926
26-27	22.36011577874201	27.4131693677293	28.625447794936665	21.601267058592025
28-29	21.673481048952787	26.912687829428737	28.753511197731886	22.660319923886586
30-31	22.591745129411134	26.994331599234663	28.826902139493964	21.58702113186024
32-33	22.726504032052937	26.686985681617603	29.13628196857249	21.450228317756967
34-35	21.554512346653144	26.940401754592934	29.671412177659427	21.833673721094492
36-37	22.206520968123872	27.158516828490963	28.87192387531433	21.763038328070834
38-39	21.640673281869827	26.774473857273605	29.311433580247524	22.27341928060904
40-41	21.77788336599157	26.661011861999494	29.404132360453435	22.1569724115555
42-43	22.13870652787035	26.58249066998296	29.462788533968542	21.81601426817815
44-45	21.63214001968434	27.19128197222848	29.26952129110292	21.907056716984265
46-47	22.358261570901934	26.57123015032683	28.40404452257774	22.666463756193494
48-49	22.124942830208052	26.643512833874	28.64481764083563	22.58672669508232
50-51	21.6492414214042	27.076991294164564	28.933108052528674	22.340659231902567
52-53	21.800298391818096	27.300490299161407	29.221941128480537	21.67727018053996
54-55	21.70633555318504	27.35964345472834	28.59851355004654	22.335507442040083
56-57	22.224623887236604	27.838890341219113	27.837762747058157	22.098723024486123
58-59	21.36256437587475	28.328130193074845	28.750531224307124	21.55877420674328
60-61	22.112443408917585	28.934682358076596	27.677384862910625	21.27548937009519
62-63	21.29344250695091	29.433571320629447	27.685172105087947	21.5878140673317
64-65	21.324956938015795	29.24633720030352	27.72377560339115	21.70493025828954
66-67	21.434799089890742	29.086414363653695	27.835135683760125	21.643650862695434
68-69	21.558555193582205	28.842988211653726	27.94065307283456	21.657803521929505
70-71	21.698460647097768	28.128917061455645	27.9753885935081	22.197233697938483
72-73	21.954895526136774	27.642178904005377	28.116600795183032	22.286324774674817
74-75	21.87037270967333	27.762664045913453	28.236294918400983	22.13066832601223
76-77	22.00812921421546	27.459742762510814	28.12488890468911	22.407239118584616
78-79	22.04138633926838	27.50597248195123	27.86788726915197	22.584753909628418
80-81	22.21486134245642	27.696340767884838	27.874512451861218	22.21428543779753
82-83	22.263569963401196	27.510625975301405	27.919557583301934	22.306246477995465
84-85	22.19402261312892	27.468270146930195	27.862538598034508	22.475168641906375
86-87	22.003014916562414	27.69128311107688	27.956061976241774	22.349639996118935
88-89	22.331366024818983	27.721003449941524	27.851439192022227	22.09619133321727
90-91	22.406565410355984	27.683898629544107	27.86507442029692	22.044461539802988
92-93	22.209437181992012	27.452620895977887	27.867755335315103	22.470186586714995
94-95	22.28250142766539	27.841722162245542	27.81572964379926	22.06004676628981
96-97	22.522845631601097	27.659183410398597	27.993605751903917	21.82436520609639
98-99	23.26556232127268	29.74717959329357	28.27344300893383	18.713815076499927
100	22.146966856942075	30.621141302987496	22.278091125345167	24.953800714725258
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1407.0
1	1256.0
2	1506.5
3	2057.5
4	2685.5
5	3868.0
6	4895.5
7	6146.5
8	7818.5
9	8649.0
10	8400.5
11	8262.5
12	9154.0
13	11338.5
14	15705.0
15	20854.5
16	24747.0
17	27108.0
18	27214.5
19	25403.5
20	23029.0
21	21197.0
22	21432.0
23	24315.0
24	30089.5
25	38891.5
26	50827.0
27	64149.5
28	76620.0
29	90668.5
30	105139.5
31	118647.0
32	134162.0
33	149882.5
34	165093.0
35	179945.5
36	195770.5
37	209877.5
38	218292.0
39	225930.5
40	237596.5
41	248321.5
42	256180.0
43	262894.5
44	267389.0
45	276195.5
46	314262.5
47	344135.5
48	336618.0
49	319780.5
50	297521.5
51	270728.0
52	246445.0
53	228883.5
54	212754.5
55	197108.5
56	180364.0
57	161927.5
58	143585.5
59	125416.5
60	105058.5
61	85328.0
62	67319.0
63	51850.0
64	39609.0
65	29613.0
66	21988.0
67	16621.5
68	12625.5
69	9305.0
70	6811.0
71	4867.0
72	3460.5
73	2393.0
74	1622.0
75	1114.5
76	716.5
77	457.0
78	292.5
79	182.5
80	108.0
81	72.0
82	55.0
83	38.5
84	22.5
85	13.5
86	10.0
87	7.0
88	3.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.021026830509596344
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	4.412916942664188E-4
26-27	3.956879148540482E-4
28-29	0.0037832987380985944
30-31	0.006279368813697681
32-33	0.004789012675913153
34-35	0.002298139120149777
36-37	0.0
38-39	7.740579733815397E-6
40-41	4.2854986750406865E-4
42-43	7.844140074378137E-6
44-45	7.905356440268588E-6
46-47	4.0677641653905056E-4
48-49	0.0017540881115471296
50-51	0.0030663121642063706
52-53	0.0
54-55	0.0029945957419152086
56-57	0.03967390980040882
58-59	0.06254373370579377
60-61	0.043396836081561516
62-63	0.014209431563283779
64-65	4.118283979703724E-4
66-67	0.0
68-69	2.640284707180545E-5
70-71	0.003191888276400001
72-73	0.005279253904217252
74-75	0.004375681120174367
76-77	0.0034160641109137694
78-79	0.008383531715545367
80-81	0.015905655768004352
82-83	0.04242962459076527
84-85	0.01952195551303295
86-87	0.0019327259847141769
88-89	0.001412322850334284
90-91	5.945059328719571E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	50.0
20-21	272.0
22-23	500.0
24-25	620.0
26-27	1826.0
28-29	4695.0
30-31	14260.0
32-33	17971.0
34-35	24442.0
36-37	37939.0
38-39	42518.0
40-41	42246.0
42-43	46916.0
44-45	55304.0
46-47	55790.0
48-49	51570.0
50-51	45109.0
52-53	41909.0
54-55	44521.0
56-57	46747.0
58-59	30730.0
60-61	64008.0
62-63	59956.0
64-65	67081.0
66-67	75341.0
68-69	77145.0
70-71	105924.0
72-73	54462.0
74-75	49305.0
76-77	50481.0
78-79	52708.0
80-81	52674.0
82-83	48797.0
84-85	48310.0
86-87	49714.0
88-89	51361.0
90-91	52759.0
92-93	52641.0
94-95	61803.0
96-97	219350.0
98-99	437886.0
100-101	4234914.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.27775731624716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.36648411143227	77.9661758568866
2	6.846938442507305	11.81476986603857
3	1.6107239391444155	4.169089473749146
4	0.5698323219875818	1.9665541914959277
5	0.2502997901181983	1.0797652274062755
6	0.12395183038761512	0.6416571564652372
7	0.06771295449749445	0.4089485298710639
8	0.04731719845017265	0.3265937411814961
9	0.02896475674393363	0.2249112827769515
>10	0.08537156315239136	1.1061046187614847
>50	0.0015810353696743995	0.09388764292671298
>100	7.15892888565355E-4	0.11253470633055944
>500	5.3081738327287453E-5	0.025028460881640076
>1k	5.308158210551614E-5	0.0639792452282638
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.128869975222726E-5	0.0	0.0	0.0
3	0.0	1.0650348304426513E-4	0.0	0.0	0.0
4	0.0	1.5214783292037876E-4	0.0	3.042956658407575E-5	1.5214783292037875E-5
5	0.0	1.6736261621241662E-4	0.0	3.042956658407575E-5	1.5214783292037875E-5
6	9.128869975222726E-5	1.6736261621241662E-4	0.0	3.042956658407575E-5	1.5214783292037875E-5
7	9.128869975222726E-5	1.6736261621241662E-4	0.0	3.042956658407575E-5	1.5214783292037875E-5
8	9.128869975222726E-5	1.825773995044545E-4	0.0	3.042956658407575E-5	1.5214783292037875E-5
9	9.128869975222726E-5	2.7386609925668175E-4	0.0	6.08591331681515E-5	1.5214783292037875E-5
10-11	1.1411087469028407E-4	2.7386609925668175E-4	0.0	9.128869975222726E-5	1.5214783292037875E-5
12-13	1.21718266336303E-4	2.7386609925668175E-4	0.0	9.128869975222726E-5	1.5214783292037875E-5
14-15	1.21718266336303E-4	3.1190305748677645E-4	0.0	9.889609139824619E-5	1.5214783292037875E-5
16-17	1.9779218279649237E-4	3.2711784077881433E-4	0.0	1.0650348304426513E-4	3.042956658407575E-5
18-19	1.9779218279649237E-4	3.4994001571687114E-4	0.0	1.0650348304426513E-4	3.042956658407575E-5
20-21	1.9779218279649237E-4	3.65154799008909E-4	0.0	1.2932565798232193E-4	3.803695823009469E-5
22-23	1.9779218279649237E-4	3.8797697394696583E-4	0.0	1.4454044127435982E-4	4.564434987611363E-5
24-25	2.0539957444251132E-4	4.1079914888502263E-4	0.0	1.7497000785843557E-4	6.08591331681515E-5
26-27	2.2822174938056815E-4	4.260139321770605E-4	0.0	2.9668827419473856E-4	6.08591331681515E-5
28-29	2.43436532672606E-4	4.260139321770605E-4	0.0	7.37916989663837E-4	6.08591331681515E-5
30-31	2.5104392431862495E-4	5.781617650974393E-4	0.0	0.001985529219610943	6.08591331681515E-5
32-33	2.5865131596464387E-4	5.781617650974393E-4	0.0	0.004739404995469798	6.08591331681515E-5
34-35	2.5865131596464387E-4	6.618430732036476E-4	0.0	0.007896472528567658	6.08591331681515E-5
36-37	2.5865131596464387E-4	7.531317729558749E-4	0.0	0.013951956278798732	6.08591331681515E-5
38-39	2.5865131596464387E-4	7.759539478939317E-4	0.0	0.032186874054306126	6.08591331681515E-5
40-41	2.5865131596464387E-4	8.52027864354121E-4	0.0	0.05195087755066333	6.08591331681515E-5
42-43	2.5865131596464387E-4	8.5963525600014E-4	0.0	0.0621447823563287	6.08591331681515E-5
44-45	2.662587076106628E-4	9.661387390444052E-4	0.0	0.07405035028234834	6.08591331681515E-5
46-47	2.7386609925668175E-4	0.0010650348304426514	0.0	0.08739371522946557	6.08591331681515E-5
48-49	2.7386609925668175E-4	0.001080249613734689	0.0	0.09851572181594526	6.08591331681515E-5
50-51	2.7386609925668175E-4	0.0013008639714692384	0.0	0.10993441667661967	6.08591331681515E-5
52-53	2.7386609925668175E-4	0.0013236861464072953	0.0	0.14740842792490894	6.846652481417044E-5
54-55	2.7386609925668175E-4	0.001331293538053314	0.0	0.1955556096525628	7.607391646018938E-5
56-57	2.7386609925668175E-4	0.001338900929699333	0.0	0.25207852958248356	7.607391646018938E-5
58-59	2.7386609925668175E-4	0.001338900929699333	0.0	0.44960445367136526	9.128869975222726E-5
60-61	3.2711784077881433E-4	0.0013997600628674845	0.0	0.6229008353676766	9.128869975222726E-5
62-63	4.1079914888502263E-4	0.0014454044127435982	0.0	0.6291541113007042	9.128869975222726E-5
64-65	4.1079914888502263E-4	0.001582337462371939	0.0	0.6351183063511832	9.128869975222726E-5
66-67	4.1840654053104155E-4	0.001582337462371939	0.0	0.6408694944355734	9.128869975222726E-5
68-69	4.260139321770605E-4	0.0016279818122480528	0.0	0.6466815416531319	9.128869975222726E-5
70-71	4.260139321770605E-4	0.0016431965955400905	0.0	0.6514970205650619	9.128869975222726E-5
72-73	4.564434987611363E-4	0.0016431965955400905	0.0	0.6549812059389385	1.5214783292037876E-4
74-75	4.564434987611363E-4	0.0016508039871861095	0.0	0.6579100517226558	1.5214783292037876E-4
76-77	4.564434987611363E-4	0.001696448337062223	0.0	0.6610823340390457	1.5214783292037876E-4
78-79	4.564434987611363E-4	0.0017344852952923177	0.0	0.6631439371751169	1.5214783292037876E-4
80-81	4.564434987611363E-4	0.0017344852952923177	0.0	0.6639046763397187	1.5214783292037876E-4
82-83	4.564434987611363E-4	0.0017344852952923177	0.0	0.6644524085382321	1.6736261621241662E-4
84-85	4.564434987611363E-4	0.0017573074702303747	0.0	0.664794741162303	1.6736261621241662E-4
86-87	4.640508904071552E-4	0.0017953444284604693	0.0	0.6649164594286392	1.6736261621241662E-4
88	4.86873065345212E-4	0.0019018479115047345	0.0	0.6649316742119313	1.6736261621241662E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7870	0.0	51.056408	1
CTTATAC	14355	0.0	43.2726	1
GTATCAA	14895	0.0	38.48486	1
TATACAC	17360	0.0	37.192387	3
TTATACA	17910	0.0	35.25189	2
TCAACGC	17965	0.0	31.42968	4
ATCAACG	17950	0.0	31.243244	3
CAACGCA	18440	0.0	30.620077	5
TATCAAC	18650	0.0	30.190277	2
AACGCAG	18900	0.0	29.852383	6
TACACAT	22445	0.0	29.030916	5
ACACATC	22340	0.0	28.065992	6
ACATCTC	23600	0.0	26.477678	8
ATACACA	25600	0.0	26.215347	4
ACGCAGA	21890	0.0	25.83292	7
CACATCT	24515	0.0	25.420202	7
CGCAGAG	22215	0.0	25.4168	8
CATCTCC	26875	0.0	23.23531	9
GTGGTAT	4050	0.0	22.10561	1
TGGTATC	3710	0.0	21.844118	2
>>END_MODULE