FastQCFastQC Report
Fri 27 May 2016
SRR2140146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5111091
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63600.051.4677281
GTATCAA119950.039.533531
TCAACGC139950.033.169864
ATCAACG140000.032.973463
CAACGCA144250.032.1810875
TATCAAC145700.032.0145532
AACGCAG148400.031.3101626
ACGCAGA172550.026.9030537
CGCAGAG172850.026.8563618
GTGGTAT29550.025.5069831
TGGTATC29750.023.0191172
GCAGAGT202800.022.953859
CTTATAC247050.021.1647111
GAGTACT122000.020.41932312-13
TATACAC272800.019.9210763
CAGAGTA200800.019.66542810-11
TTATACA273200.019.5014782
GTACATG146350.019.394171
TGCTTGA168050.019.26423654-55
TACATGG144200.019.1755682
Produced by FastQC (version 0.11.5)