##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2140146_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5111091 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.481782656579583 31.0 18.0 32.0 18.0 33.0 2 29.238851939830457 31.0 28.0 33.0 18.0 33.0 3 29.638364490086364 31.0 29.0 33.0 25.0 33.0 4 30.709365769460963 33.0 31.0 33.0 28.0 33.0 5 31.754883057257246 33.0 32.0 33.0 30.0 33.0 6 34.51558346349145 37.0 34.0 38.0 29.0 38.0 7 36.01232222239831 38.0 36.0 38.0 33.0 38.0 8 35.788609516050485 38.0 36.0 38.0 31.0 38.0 9 36.4123827965497 38.0 37.0 38.0 33.0 38.0 10-11 36.77020131318343 38.0 38.0 38.0 34.0 38.0 12-13 36.98018554159963 38.0 38.0 38.0 35.0 38.0 14-15 37.02388443093656 38.0 38.0 38.0 35.5 38.0 16-17 37.089708831245616 38.0 38.0 38.0 36.0 38.0 18-19 37.17032537671507 38.0 38.0 38.0 36.0 38.0 20-21 37.224463991765674 38.0 38.0 38.0 36.0 38.0 22-23 37.18524284719714 38.0 38.0 38.0 36.0 38.0 24-25 37.25924821525824 38.0 38.0 38.0 36.0 38.0 26-27 37.24409958145785 38.0 38.0 38.0 36.0 38.0 28-29 37.227632480303555 38.0 38.0 38.0 36.0 38.0 30-31 37.236099484641144 38.0 38.0 38.0 36.0 38.0 32-33 37.20921115348324 38.0 38.0 38.0 36.0 38.0 34-35 37.11474092214908 38.0 38.0 38.0 36.0 38.0 36-37 37.13955437606235 38.0 38.0 38.0 36.0 38.0 38-39 37.08486835047138 38.0 38.0 38.0 36.0 38.0 40-41 37.15127113187698 38.0 38.0 38.0 36.0 38.0 42-43 37.128476480017184 38.0 38.0 38.0 36.0 38.0 44-45 37.08885126397823 38.0 38.0 38.0 36.0 38.0 46-47 37.048356134558475 38.0 38.0 38.0 36.0 38.0 48-49 36.99066682722501 38.0 38.0 38.0 36.0 38.0 50-51 36.95178466366333 38.0 38.0 38.0 36.0 38.0 52-53 36.85712726711094 38.0 38.0 38.0 35.5 38.0 54-55 36.881137860840894 38.0 38.0 38.0 35.5 38.0 56-57 36.833846812135604 38.0 38.0 38.0 35.0 38.0 58-59 36.8047661384879 38.0 38.0 38.0 35.0 38.0 60-61 36.78188468920142 38.0 38.0 38.0 35.0 38.0 62-63 36.78614670593809 38.0 38.0 38.0 35.0 38.0 64-65 36.79506669658924 38.0 38.0 38.0 35.0 38.0 66-67 36.781054452618534 38.0 38.0 38.0 35.0 38.0 68-69 36.750323857866356 38.0 38.0 38.0 35.0 38.0 70-71 36.74856633890723 38.0 38.0 38.0 35.0 38.0 72-73 36.75580090829637 38.0 38.0 38.0 35.0 38.0 74-75 36.68699799012846 38.0 38.0 38.0 34.5 38.0 76-77 36.68603616801997 38.0 38.0 38.0 34.0 38.0 78-79 36.681651219435196 38.0 38.0 38.0 34.0 38.0 80-81 36.65241103393471 38.0 38.0 38.0 34.0 38.0 82-83 36.56825554168879 38.0 38.0 38.0 34.0 38.0 84-85 36.58578462803743 38.0 38.0 38.0 34.0 38.0 86-87 36.57582124387299 38.0 38.0 38.0 34.0 38.0 88-89 36.55030170512603 38.0 38.0 38.0 34.0 38.0 90-91 36.565225378286605 38.0 38.0 38.0 34.0 38.0 92-93 36.53418802910685 38.0 38.0 38.0 34.0 38.0 94-95 36.43395896499801 38.0 38.0 38.0 34.0 38.0 96-97 36.397297824470655 38.0 37.0 38.0 34.0 38.0 98-99 36.451569037524465 38.0 37.0 38.0 34.0 38.0 100 34.77999211309958 38.0 34.0 38.0 28.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 2.0 18 2.0 19 8.0 20 32.0 21 132.0 22 450.0 23 1394.0 24 3390.0 25 6772.0 26 12024.0 27 19271.0 28 28834.0 29 40746.0 30 55624.0 31 72531.0 32 96369.0 33 135712.0 34 210010.0 35 395265.0 36 1130770.0 37 2901753.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.59763365590634 17.82795493173571 14.16116441675564 27.413246995602307 2 13.839468474737599 19.766056557531677 43.48524615058265 22.90922881714808 3 17.912516134030877 25.048487690788523 30.85090834813937 26.188087827041233 4 12.083975808687422 18.31346771168817 38.381864067769484 31.220692411854923 5 13.481583481882831 36.498371874028464 35.931858775357355 14.088185868731355 6 29.69311248811653 37.61609801116826 18.52594680861679 14.16484269209842 7 26.27079032637063 32.39249702265133 22.181076408148474 19.155636242829562 8 24.060694673602956 36.689407408320456 21.217681313050385 18.032216605026207 9 25.013074507967087 16.846305416984357 20.49716978234197 37.64345029270659 10-11 23.998672690429498 26.449881248445784 28.82003079186029 20.731415269264428 12-13 25.10499226094781 23.564655765275948 28.690078106611683 22.640273867164566 14-15 22.308417126597824 25.071017127263044 25.891135571642142 26.729430174496994 16-17 22.239273376271328 28.777036057467964 26.398757134240032 22.58493343202068 18-19 22.334742230181384 27.246374599865277 28.23908828858653 22.179794881366817 20-21 22.739610161620526 26.384062032290846 28.198701412664466 22.677626393424163 22-23 22.388614572124386 27.040635785722273 28.033517789902263 22.53723185225108 24-25 22.65837773019674 26.99374954259457 28.194529294102594 22.153343433106095 26-27 22.488398130862706 27.244631181604845 28.24592537737265 22.0210453101598 28-29 22.409371009608854 26.58116893669059 28.493424089911823 22.516035963788735 30-31 22.10461021094107 26.218434453570833 29.029443306340973 22.647512029147123 32-33 21.73542764028159 27.023118136276786 28.575988311795353 22.665465911646265 34-35 22.38652534611979 26.627649341479177 28.626988367127304 22.358836945273726 36-37 21.956280460845655 26.90102592662521 29.05195299713149 22.090740615397646 38-39 21.867671050656305 26.49424321747525 28.908373655499737 22.729712076368706 40-41 22.226999578331434 26.2928238404614 29.0975793026681 22.38259727853907 42-43 22.558794591075635 26.200283233040487 28.974050112797396 22.266872063086485 44-45 21.94292745729623 26.826488784609538 28.857274255151655 22.373309502942583 46-47 22.661083429924734 26.149597801896977 28.250681796087896 22.938636972090393 48-49 22.400669867686286 26.383359866455336 28.278249527362654 22.93772073849572 50-51 21.895777647454405 26.683294672734327 28.51606060064481 22.90486707916646 52-53 21.982118929927307 26.678946526363507 28.991534229726984 22.347400313982206 54-55 22.0628982137446 26.54365662567525 28.571392832233382 22.822052328346768 56-57 22.531201736444462 26.712223984639145 27.881224694243855 22.875349584672538 58-59 21.544153993169953 27.0223344917307 28.928226858133023 22.505284656966325 60-61 22.301696269934617 28.11144997188076 27.84041392531669 21.74643983286794 62-63 21.213452685812488 28.921787963020297 27.82660908274289 22.038150268424324 64-65 21.04942849264983 29.04401541195832 27.593733058042858 22.312823037348995 66-67 21.21857210542741 29.397642867990797 27.161769677306708 22.222015349275082 68-69 21.48008505660951 29.031859587041232 27.206080386899405 22.281974969449852 70-71 21.59958944023341 27.953993680009674 27.398746143340414 23.047670736416507 72-73 22.007659769309743 26.907825509326855 27.629022843032047 23.45549187833135 74-75 22.107842565604336 26.962287754718915 27.75644758958965 23.173422090087097 76-77 22.42231781796839 26.85390046640224 27.120796704361517 23.602985011267847 78-79 22.65925756600586 26.936216390952527 26.652558493160434 23.75196754988118 80-81 22.453662329305786 27.30512449530203 26.83931366720225 23.401899508189935 82-83 22.4832074490737 27.028032593022573 26.916723530519683 23.57203642738405 84-85 22.58580222268341 27.370342269472204 26.869946132016103 23.173909375828284 86-87 23.00765834171632 27.28284840981196 26.915322970672445 22.79417027779927 88-89 22.6957052789831 27.512769272253713 27.234026034996774 22.557499413766415 90-91 22.791497845163754 27.04369734905388 27.0510094024622 23.113795403320164 92-93 22.741630879258345 26.682317571425422 27.259720842642253 23.31633070667398 94-95 22.02695042195913 27.186341740861792 27.804239477973958 22.98246835920512 96-97 22.183419287016214 27.00201972284239 28.080646649060135 22.733914341081256 98-99 23.34991970018426 28.90836613591037 28.33855272835225 19.40316143555312 100 22.415506218082328 29.925753529596427 22.038614836308096 25.620125416013146 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1104.0 1 1023.5 2 1156.5 3 1532.0 4 1985.0 5 2828.0 6 3613.5 7 4574.0 8 5733.0 9 6225.5 10 6061.0 11 5920.5 12 6516.5 13 8220.0 14 11477.5 15 15218.0 16 18152.0 17 20101.0 18 20241.5 19 18479.0 20 16203.0 21 14334.0 22 13713.0 23 15099.0 24 18555.5 25 23848.5 26 31133.5 27 39710.0 28 48315.5 29 58116.5 30 68133.0 31 78024.5 32 89054.0 33 100351.0 34 112254.5 35 124175.5 36 137328.0 37 149371.5 38 157957.0 39 166238.0 40 176001.0 41 185346.5 42 192660.0 43 200531.0 44 218993.0 45 251702.5 46 257291.0 47 242789.0 48 235169.0 49 223508.5 50 214909.0 51 207972.0 52 199614.0 53 188988.5 54 177837.5 55 166068.5 56 151781.0 57 136296.0 58 120818.5 59 104978.0 60 87551.5 61 70273.0 62 55393.0 63 42920.5 64 32456.5 65 23872.5 66 17498.5 67 13456.0 68 10352.0 69 7659.5 70 5705.0 71 4167.0 72 2959.5 73 2067.5 74 1431.5 75 921.0 76 574.5 77 370.5 78 244.5 79 166.0 80 108.5 81 67.5 82 44.0 83 31.0 84 22.0 85 13.5 86 9.0 87 7.5 88 6.0 89 3.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01891963966206041 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.674730328009197E-4 26-27 2.739859535229799E-4 28-29 0.0039743254659309266 30-31 0.006223190666115629 32-33 0.004774237038118353 34-35 0.0023746427566744954 36-37 0.0 38-39 0.0 40-41 4.5963661129511005E-4 42-43 0.0 44-45 1.0134265847939598E-5 46-47 2.657768457869719E-4 48-49 0.0017319598342019592 50-51 0.0027119179442207314 52-53 0.0 54-55 0.0031375050684778723 56-57 0.039951474084219375 58-59 0.06144767207697099 60-61 0.04205865448247834 62-63 0.01354873633120151 64-65 3.8517977998744955E-4 66-67 0.0 68-69 0.0 70-71 0.003075760322937004 72-73 0.005357858505866572 74-75 0.004344355573156548 76-77 0.003345175253731543 78-79 0.007999400103212055 80-81 0.016130799622078407 82-83 0.041748697059607334 84-85 0.01844945950271758 86-87 0.001874045974579473 88-89 0.0012939085106588154 90-91 6.165302614014325E-5 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 37.0 20-21 179.0 22-23 411.0 24-25 495.0 26-27 1387.0 28-29 3942.0 30-31 11573.0 32-33 14364.0 34-35 19203.0 36-37 29439.0 38-39 17864.0 40-41 32387.0 42-43 35881.0 44-45 41802.0 46-47 42110.0 48-49 39015.0 50-51 19782.0 52-53 3601.0 54-55 3415.0 56-57 3742.0 58-59 21825.0 60-61 41965.0 62-63 41944.0 64-65 52749.0 66-67 59294.0 68-69 60559.0 70-71 86853.0 72-73 42265.0 74-75 38501.0 76-77 40128.0 78-79 41115.0 80-81 41328.0 82-83 38858.0 84-85 38086.0 86-87 38874.0 88-89 40682.0 90-91 41605.0 92-93 42305.0 94-95 50435.0 96-97 176716.0 98-99 396909.0 100-101 3357466.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.2972638874542 #Duplication Level Percentage of deduplicated Percentage of total 1 90.96068628880482 79.40619034337732 2 6.609759900839905 11.540279085926688 3 1.4236149994366696 3.728330828398827 4 0.4871417471774317 1.7010456661577502 5 0.2044746476526185 0.8925038637212427 6 0.10457702003682076 0.5477572628830757 7 0.06036408623965924 0.3688733696052003 8 0.036175351993313 0.25264073993454306 9 0.02653482731661104 0.20847760402195997 >10 0.08430973857450755 1.084662088314921 >50 0.0014092001033698877 0.08378934044282657 >100 9.199092360975864E-4 0.1616361725143053 >500 3.228258820452168E-5 0.023813634701338227 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.9565294376484394E-5 0.0 0.0 0.0 3 0.0 1.9565294376484394E-5 0.0 0.0 0.0 4 0.0 1.9565294376484394E-5 0.0 1.9565294376484394E-5 0.0 5 0.0 3.913058875296879E-5 0.0 1.9565294376484394E-5 0.0 6 1.5652235501187515E-4 3.913058875296879E-5 0.0 1.9565294376484394E-5 1.9565294376484394E-5 7 1.5652235501187515E-4 3.913058875296879E-5 0.0 1.9565294376484394E-5 1.9565294376484394E-5 8 1.5652235501187515E-4 5.8695883129453185E-5 0.0 1.9565294376484394E-5 3.913058875296879E-5 9 1.5652235501187515E-4 7.826117750593758E-5 0.0 1.9565294376484394E-5 5.8695883129453185E-5 10-11 1.7608764938835956E-4 7.826117750593758E-5 0.0 1.9565294376484394E-5 1.0760911907066417E-4 12-13 1.9565294376484393E-4 7.826117750593758E-5 0.0 2.9347941564726593E-5 1.5652235501187515E-4 14-15 2.1521823814132834E-4 1.3695706063539075E-4 0.0 3.913058875296879E-5 1.5652235501187515E-4 16-17 3.228273572119925E-4 1.3695706063539075E-4 0.0 5.8695883129453185E-5 1.6630500220011737E-4 18-19 3.326100044002347E-4 1.5652235501187515E-4 0.0 7.826117750593758E-5 1.9565294376484393E-4 20-21 3.423926515884769E-4 1.9565294376484393E-4 0.0 7.826117750593758E-5 1.9565294376484393E-4 22-23 3.619579459649613E-4 2.4456617970605493E-4 0.0 1.0760911907066417E-4 1.9565294376484393E-4 24-25 3.717405931532035E-4 3.130447100237503E-4 0.0 1.4673970782363294E-4 2.1521823814132834E-4 26-27 3.717405931532035E-4 3.326100044002347E-4 0.0 2.641314740825393E-4 2.3478353251781274E-4 28-29 3.9130588752968787E-4 3.423926515884769E-4 0.0 8.608729525653135E-4 2.3478353251781274E-4 30-31 3.9130588752968787E-4 3.9130588752968787E-4 0.0 0.0026510973880136354 2.3478353251781274E-4 32-33 3.9130588752968787E-4 3.9130588752968787E-4 0.0 0.006720678618322389 2.543488268942971E-4 34-35 4.206538290944145E-4 4.4021912347089886E-4 0.0 0.012697876050338373 2.9347941564726593E-4 36-37 4.304364762826567E-4 4.891323594121099E-4 0.0 0.02550336121974741 3.032620628355081E-4 38-39 4.304364762826567E-4 4.891323594121099E-4 0.0 0.19993774323329402 3.130447100237503E-4 40-41 4.304364762826567E-4 5.086976537885942E-4 0.0 0.3812199782786102 3.326100044002347E-4 42-43 4.304364762826567E-4 5.282629481650786E-4 0.0 0.40824943245972334 3.717405931532035E-4 44-45 4.304364762826567E-4 6.358720672357428E-4 0.0 0.443535440867713 3.815232403414457E-4 46-47 4.304364762826567E-4 7.239158919299226E-4 0.0 0.48194211372875184 3.9130588752968787E-4 48-49 4.304364762826567E-4 7.239158919299226E-4 0.0 0.5199476980550728 4.108711819061723E-4 50-51 4.304364762826567E-4 9.097861885065243E-4 0.0 0.5576598029657465 4.108711819061723E-4 52-53 4.304364762826567E-4 9.195688356947666E-4 0.0 1.0359823372348487 4.108711819061723E-4 54-55 4.4021912347089886E-4 9.293514828830088E-4 0.0 1.6650358993803867 4.108711819061723E-4 56-57 4.5000177065914105E-4 9.39134130071251E-4 0.0 2.3460744486842438 4.108711819061723E-4 58-59 4.5000177065914105E-4 9.489167772594932E-4 0.0 3.033745632781729 4.108711819061723E-4 60-61 4.7934971222386767E-4 9.97830013200704E-4 0.0 3.215174607534869 4.108711819061723E-4 62-63 5.673935369180475E-4 0.0010271779547654306 0.0 3.2221594176272736 4.108711819061723E-4 64-65 5.673935369180475E-4 0.0011739176625890637 0.0 3.228224658883984 4.108711819061723E-4 66-67 6.45654714423985E-4 0.0011739176625890637 0.0 3.2339083769003527 4.108711819061723E-4 68-69 6.652200088004694E-4 0.001183700309777306 0.0 3.2390540493213678 4.206538290944145E-4 70-71 6.945679503651959E-4 0.0012130482513420325 0.0 3.2436029802639004 4.5000177065914105E-4 72-73 7.43481186306407E-4 0.0012326135457185168 0.0 3.24722255972355 4.695670650356255E-4 74-75 7.43481186306407E-4 0.0012521788400950012 0.0 3.2500301794665756 4.695670650356255E-4 76-77 7.43481186306407E-4 0.0013108747232244543 0.0 3.2525149718523894 4.891323594121099E-4 78-79 7.43481186306407E-4 0.0013500053119774233 0.0 3.2544225880540965 5.086976537885942E-4 80-81 7.43481186306407E-4 0.0013500053119774233 0.0 3.25529324365385 5.282629481650786E-4 82-83 7.43481186306407E-4 0.0013597879591656656 0.0 3.255743245424509 5.576108897298053E-4 84-85 7.630464806828914E-4 0.0013695706063539077 0.0 3.2560073768985918 5.673935369180475E-4 86-87 7.630464806828914E-4 0.0014087011951068765 0.0 3.256134551312039 5.673935369180475E-4 88 7.630464806828914E-4 0.0014673970782363296 0.0 3.256154116606415 5.869588312945319E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6360 0.0 51.467728 1 GTATCAA 11995 0.0 39.53353 1 TCAACGC 13995 0.0 33.16986 4 ATCAACG 14000 0.0 32.97346 3 CAACGCA 14425 0.0 32.181087 5 TATCAAC 14570 0.0 32.014553 2 AACGCAG 14840 0.0 31.310162 6 ACGCAGA 17255 0.0 26.903053 7 CGCAGAG 17285 0.0 26.856361 8 GTGGTAT 2955 0.0 25.506983 1 TGGTATC 2975 0.0 23.019117 2 GCAGAGT 20280 0.0 22.95385 9 CTTATAC 24705 0.0 21.164711 1 GAGTACT 12200 0.0 20.419323 12-13 TATACAC 27280 0.0 19.921076 3 CAGAGTA 20080 0.0 19.665428 10-11 TTATACA 27320 0.0 19.501478 2 GTACATG 14635 0.0 19.39417 1 TGCTTGA 16805 0.0 19.264236 54-55 TACATGG 14420 0.0 19.175568 2 >>END_MODULE