FastQCFastQC Report
Fri 27 May 2016
SRR2140145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5427639
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83250.051.9346471
CTTATAC91350.045.3845521
TATACAC115550.040.0455173
GTATCAA151350.038.182011
TTATACA117400.038.1248472
TATCAAC174600.032.6615032
TCAACGC177800.032.1150444
ATCAACG176700.032.0995333
CAACGCA182350.031.3137075
GCGCAGA14200.031.281781
AACGCAG188100.030.4239436
TACACAT158550.029.158185
GATCAAC27150.028.3590771
ATACACA172700.028.0181964
ACACATC163900.027.5096286
GTGGTAT36550.027.0843871
ACGCAGA214150.026.7823167
ACATCTC173900.026.146618
TGGTATC35650.025.8694672
CGCAGAG221600.025.8246568
Produced by FastQC (version 0.11.5)