##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140145_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5427639
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.57803567997061	18.0	18.0	32.0	18.0	33.0
2	26.66133027638721	27.0	25.0	30.0	18.0	33.0
3	25.84112834328149	27.0	18.0	30.0	18.0	31.0
4	30.335357417838583	31.0	29.0	33.0	27.0	33.0
5	32.10286645814138	33.0	32.0	33.0	31.0	33.0
6	36.52012302218331	38.0	37.0	38.0	34.0	38.0
7	36.87158246891512	38.0	38.0	38.0	35.0	38.0
8	37.09940620590279	38.0	38.0	38.0	36.0	38.0
9	37.27754940960517	38.0	38.0	38.0	36.0	38.0
10-11	37.2603062768176	38.0	38.0	38.0	36.0	38.0
12-13	37.362415868115036	38.0	38.0	38.0	37.0	38.0
14-15	37.350395908792024	38.0	38.0	38.0	37.0	38.0
16-17	37.380853074421495	38.0	38.0	38.0	37.0	38.0
18-19	37.38842303255615	38.0	38.0	38.0	37.0	38.0
20-21	37.40326683273811	38.0	38.0	38.0	37.0	38.0
22-23	37.34355206540161	38.0	38.0	38.0	37.0	38.0
24-25	37.4014992665491	38.0	38.0	38.0	37.0	38.0
26-27	37.366575909994836	38.0	38.0	38.0	37.0	38.0
28-29	37.342898151851315	38.0	38.0	38.0	37.0	38.0
30-31	37.35136887466275	38.0	38.0	38.0	37.0	38.0
32-33	37.32100014017935	38.0	38.0	38.0	37.0	38.0
34-35	37.226099560914214	38.0	38.0	38.0	37.0	38.0
36-37	37.24441220353068	38.0	38.0	38.0	37.0	38.0
38-39	37.21074507842212	38.0	38.0	38.0	37.0	38.0
40-41	37.26596318752144	38.0	38.0	38.0	37.0	38.0
42-43	37.24189375303217	38.0	38.0	38.0	37.0	38.0
44-45	37.20296914200746	38.0	38.0	38.0	37.0	38.0
46-47	37.1599840088418	38.0	38.0	38.0	37.0	38.0
48-49	37.10053767674496	38.0	38.0	38.0	36.0	38.0
50-51	37.06378976563427	38.0	38.0	38.0	36.0	38.0
52-53	36.97154124825502	38.0	38.0	38.0	36.0	38.0
54-55	37.00695144678262	38.0	38.0	38.0	36.0	38.0
56-57	36.96795816303761	38.0	38.0	38.0	36.0	38.0
58-59	36.94291214535843	38.0	38.0	38.0	36.0	38.0
60-61	36.93554955689967	38.0	38.0	38.0	36.0	38.0
62-63	36.950117093914706	38.0	38.0	38.0	36.0	38.0
64-65	36.957323874888885	38.0	38.0	38.0	36.0	38.0
66-67	36.949453175798176	38.0	38.0	38.0	36.0	38.0
68-69	36.92859677314465	38.0	38.0	38.0	36.0	38.0
70-71	36.931133214398514	38.0	38.0	38.0	36.0	38.0
72-73	36.94466424092501	38.0	38.0	38.0	36.0	38.0
74-75	36.892984013285535	38.0	38.0	38.0	35.5	38.0
76-77	36.901108436940106	38.0	38.0	38.0	35.5	38.0
78-79	36.90846836067935	38.0	38.0	38.0	35.5	38.0
80-81	36.89225516506289	38.0	38.0	38.0	35.0	38.0
82-83	36.845424047825176	38.0	38.0	38.0	35.0	38.0
84-85	36.845933442536335	38.0	38.0	38.0	35.0	38.0
86-87	36.84224981826862	38.0	38.0	38.0	35.0	38.0
88-89	36.82438097765825	38.0	38.0	38.0	35.0	38.0
90-91	36.836824896583465	38.0	38.0	38.0	35.0	38.0
92-93	36.809077866363424	38.0	38.0	38.0	35.0	38.0
94-95	36.746725990963355	38.0	38.0	38.0	35.0	38.0
96-97	36.77150660120728	38.0	38.0	38.0	35.0	38.0
98-99	36.80036622869636	38.0	38.0	38.0	35.0	38.0
100	35.283487467016	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	6.0
19	27.0
20	92.0
21	223.0
22	582.0
23	1279.0
24	2959.0
25	5883.0
26	10164.0
27	17020.0
28	26362.0
29	38060.0
30	50362.0
31	65106.0
32	84325.0
33	114361.0
34	177141.0
35	358384.0
36	1249894.0
37	3225409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.09347213401628	14.337707426746693	10.313213535388039	22.255606903848985
2	14.485304685916454	19.076802594483038	42.422266855847724	24.01562586375279
3	18.972356120220965	25.605534929644364	30.521724086660885	24.900384863473786
4	12.513931011255538	18.196106999747037	38.466375527185946	30.82358646181148
5	13.411927359207198	37.65132500521866	34.98355362248668	13.953194013087458
6	29.44423901442229	37.510435016035515	18.70780278496783	14.33752318457436
7	26.226025717627866	33.068374665301064	22.18649766500683	18.51910195206424
8	24.15534636699309	36.58898463954585	21.205758157460362	18.0499108360007
9	25.39767291081813	17.207223988183443	20.71720318908461	36.67789991191382
10-11	24.234588925313567	26.609765314163305	28.45495067007957	20.70069509044356
12-13	25.375913910265584	24.00751044791299	28.501296788529967	22.11527885329146
14-15	22.535056218735257	25.370119493945708	25.905904943199058	26.188919344119977
16-17	22.0499834274166	29.02787012916666	26.494383653739682	22.427762789677057
18-19	22.194530623720553	27.43703109215628	28.51907615816011	21.849362125963058
20-21	22.857610047944963	26.52642914817891	28.52477924083074	22.091181563045392
22-23	22.535571207548134	27.064703548857054	28.40051126211986	21.999213981474945
24-25	22.69546770032125	27.103544932538632	28.65818198703583	21.542805380104287
26-27	22.69905201042346	27.247181279048764	28.670888758657036	21.382877951870732
28-29	22.581340738902433	26.96531276077841	28.630579390397564	21.822767109921593
30-31	22.38805942595524	27.31092475758401	28.94368933831165	21.3573264781491
32-33	22.453580337382217	27.388405909740264	28.69186670515696	21.46614704772056
34-35	22.811861869707197	26.871097901269138	28.892483998570412	21.424556230453256
36-37	22.041701852291872	27.421837574733427	28.68608711207295	21.85037346090175
38-39	21.924890511838978	26.693330907037538	29.17074149913188	22.2110370819916
40-41	22.187510831896244	26.557944257916766	29.234904267996153	22.019640642190836
42-43	22.508625446488946	26.521723913813823	29.009860180176204	21.95979045952103
44-45	22.05330069748765	26.978487419065583	28.90149722439343	22.06671465905334
46-47	22.673921400639323	26.530216809669504	28.167235697038656	22.62862609265252
48-49	22.37524633455549	26.702128553278893	28.234350490480992	22.68827462168463
50-51	22.041530645540107	26.949975488612054	28.55199369797241	22.456500167875422
52-53	22.177835770282197	27.023595137865737	28.846448899696103	21.952120192155967
54-55	22.20941326442887	27.01619126906249	28.279629678501824	22.494765788006816
56-57	22.590486585859736	27.549595574042613	27.604987555698063	22.254930284399585
58-59	21.863949989721927	28.126046288222827	28.31984109476182	21.690162627293425
60-61	22.35994367114624	28.809824939260782	27.262861869381293	21.56736952021168
62-63	21.784596043897132	29.33441549711485	27.24562428977831	21.635364169209712
64-65	21.80297665052098	29.219279044512746	27.289670320306193	21.68807398466008
66-67	21.989882526714453	29.04052117305172	27.196146711292524	21.7734495889413
68-69	22.03613755752457	28.64552469205308	27.409426082980364	21.90891166744199
70-71	22.179466331696712	28.071810145838487	27.618128153889838	22.13059536857496
72-73	22.43906342220516	27.60265421331518	27.70261274469061	22.255669619789046
74-75	22.244096050182723	27.624625591529337	27.758994101442582	22.372284256845358
76-77	22.4882784069553	27.403981303207413	27.66055683376433	22.44718345607296
78-79	22.447551889818236	27.528350406812528	27.631360967645275	22.39273673572396
80-81	22.44618479790697	27.54842144952063	27.586310114869082	22.41908363770331
82-83	22.497337249365508	27.463787463927076	27.69123377699907	22.347641509708353
84-85	22.579723390918822	27.44620305829922	27.63324932341657	22.34082422736539
86-87	22.47748180941439	27.430588453482724	27.752985833986223	22.33894390311667
88-89	22.56337740637185	27.44632679286279	27.640650387413196	22.349645413352164
90-91	22.60324020287052	27.40682291503943	27.692937580332384	22.296999301757666
92-93	22.638651001867515	27.474335132297828	27.594935283305084	22.292078582529577
94-95	22.513888151348077	27.555337532302744	27.60126105243587	22.329513263913313
96-97	22.636586081453956	27.588579408169846	27.690821605977185	22.084012904399017
98-99	23.642635491832888	29.565148141977332	27.8773251953478	18.914891170841976
100	22.62084838528238	30.28247606801177	21.86748689909204	25.229188647613814
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	682.0
1	775.5
2	1260.0
3	1770.5
4	2283.5
5	3384.5
6	4347.0
7	5141.5
8	6687.5
9	7780.5
10	7419.0
11	7031.0
12	7757.5
13	9701.0
14	13126.5
15	17208.0
16	21540.0
17	25889.5
18	27345.5
19	25239.0
20	21998.5
21	18897.5
22	17343.0
23	18531.0
24	22223.0
25	28250.0
26	36736.5
27	46534.0
28	56405.5
29	67521.5
30	79096.5
31	90171.0
32	102028.0
33	113845.5
34	126212.0
35	137680.0
36	149712.5
37	162018.0
38	172264.5
39	181214.0
40	190446.0
41	199515.5
42	210107.0
43	231724.5
44	258357.0
45	269761.5
46	262833.5
47	252828.0
48	242287.0
49	231042.0
50	224416.5
51	219473.5
52	211895.0
53	201917.0
54	190566.5
55	176770.0
56	161859.0
57	146121.0
58	130376.0
59	114408.5
60	96155.0
61	77843.0
62	61935.0
63	48215.5
64	36866.5
65	27763.5
66	20405.0
67	15626.0
68	12083.0
69	8856.0
70	6495.0
71	4772.0
72	3422.0
73	2352.5
74	1588.5
75	1108.5
76	762.0
77	488.0
78	305.5
79	196.0
80	124.0
81	76.5
82	50.0
83	35.5
84	25.5
85	16.5
86	10.0
87	6.5
88	6.0
89	5.5
90	4.5
91	2.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013615496535418069
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.316764253241584E-4
26-27	3.317215352514947E-4
28-29	0.003917860623168458
30-31	0.006100987870848455
32-33	0.004477996936457963
34-35	0.001976735035094935
36-37	0.0
38-39	0.0
40-41	4.627884116270583E-4
42-43	0.0
44-45	0.0
46-47	3.9681629447717857E-4
48-49	0.0015824552985682881
50-51	0.0025614138373880515
52-53	0.0
54-55	0.003108841334834507
56-57	0.04072377315478398
58-59	0.06552739244033433
60-61	0.04395620155316693
62-63	0.014021486376823104
64-65	3.4461282435898884E-4
66-67	0.0
68-69	1.072369154073389E-5
70-71	0.0034198702083016804
72-73	0.0054336771563564396
74-75	0.004514882068263005
76-77	0.0035757984097961985
78-79	0.008453420513694541
80-81	0.016725318471704168
82-83	0.045073378553235253
84-85	0.02097165456608461
86-87	0.002086977886453462
88-89	0.0014975391837117576
90-91	1.2109633843423403E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	34.0
20-21	161.0
22-23	380.0
24-25	559.0
26-27	1714.0
28-29	4617.0
30-31	12409.0
32-33	15424.0
34-35	21149.0
36-37	31761.0
38-39	36614.0
40-41	35884.0
42-43	40656.0
44-45	47785.0
46-47	48371.0
48-49	44744.0
50-51	38351.0
52-53	35783.0
54-55	38034.0
56-57	39107.0
58-59	41550.0
60-61	45682.0
62-63	46619.0
64-65	56987.0
66-67	64636.0
68-69	64351.0
70-71	83701.0
72-73	46079.0
74-75	41363.0
76-77	43223.0
78-79	44875.0
80-81	44563.0
82-83	42248.0
84-85	41092.0
86-87	42742.0
88-89	44179.0
90-91	44745.0
92-93	44208.0
94-95	52120.0
96-97	162763.0
98-99	357037.0
100-101	3479339.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.26786893341571
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.52722328041362	80.78912948355025
2	6.219663333727047	10.979928559027808
3	1.3612644202471789	3.6046772829030043
4	0.43565063826136663	1.5381581375525286
5	0.1951908430042308	0.8614539873650184
6	0.0935179573768637	0.49527784827970583
7	0.050431622024470824	0.3116044262068886
8	0.0323727593048522	0.2285979580266967
9	0.017906837511454034	0.14225385478256972
>10	0.06497097222158336	0.8501448519575558
>50	0.0011049865108616668	0.06769581548965345
>100	6.72489513270435E-4	0.11684518627552647
>500	2.985988319327133E-5	0.014232608582782195
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.842421723331268E-5	1.842421723331268E-5
4	0.0	3.684843446662536E-5	0.0	1.842421723331268E-5	3.684843446662536E-5
5	0.0	3.684843446662536E-5	0.0	1.842421723331268E-5	3.684843446662536E-5
6	0.0	3.684843446662536E-5	0.0	1.842421723331268E-5	5.527265169993804E-5
7	0.0	3.684843446662536E-5	0.0	1.842421723331268E-5	5.527265169993804E-5
8	0.0	5.527265169993804E-5	0.0	1.842421723331268E-5	7.369686893325072E-5
9	0.0	7.369686893325072E-5	0.0	3.684843446662536E-5	7.369686893325072E-5
10-11	0.0	7.369686893325072E-5	0.0	3.684843446662536E-5	7.369686893325072E-5
12-13	0.0	7.369686893325072E-5	0.0	7.369686893325072E-5	7.369686893325072E-5
14-15	0.0	1.842421723331268E-4	0.0	9.21210861665634E-5	1.1054530339987608E-4
16-17	1.842421723331268E-5	1.842421723331268E-4	0.0	1.0133319478321975E-4	1.4739373786650144E-4
18-19	1.842421723331268E-5	2.303027154164085E-4	0.0	1.2896952063318876E-4	1.658179550998141E-4
20-21	1.842421723331268E-5	2.763632584996902E-4	0.0	1.4739373786650144E-4	1.9345428094978313E-4
22-23	2.763632584996902E-5	2.9478747573300287E-4	0.0	1.7503006371647045E-4	2.303027154164085E-4
24-25	3.684843446662536E-5	3.500601274329409E-4	0.0	2.026663895664395E-4	2.6715114988303386E-4
26-27	3.684843446662536E-5	3.684843446662536E-4	0.0	3.869085618995663E-4	2.8557536711634653E-4
28-29	3.684843446662536E-5	3.684843446662536E-4	0.0	0.001298907314948544	3.1321169296631555E-4
30-31	3.684843446662536E-5	4.6981753944947335E-4	0.0	0.0034269044053961586	3.4084801881628456E-4
32-33	3.684843446662536E-5	5.343022997660677E-4	0.0	0.007378899001941728	3.684843446662536E-4
34-35	3.684843446662536E-5	6.264233859326311E-4	0.0	0.013164103213201911	3.684843446662536E-4
36-37	3.684843446662536E-5	7.185444720991945E-4	0.0	0.02157475838020915	3.684843446662536E-4
38-39	3.684843446662536E-5	7.185444720991945E-4	0.0	0.04195194264025297	3.684843446662536E-4
40-41	3.684843446662536E-5	7.553929065658198E-4	0.0	0.06461372983722757	3.684843446662536E-4
42-43	3.684843446662536E-5	7.830292324157888E-4	0.0	0.07791601467967932	3.776964532829099E-4
44-45	3.684843446662536E-5	8.290897754990706E-4	0.0	0.09293175172482915	3.8690856189956625E-4
46-47	3.684843446662536E-5	8.475139927323833E-4	0.0	0.10815936726816208	3.961206705162226E-4
48-49	3.684843446662536E-5	8.567261013490397E-4	0.0	0.12351595233212821	4.2375699636619166E-4
50-51	4.6060543083281704E-5	9.949077305988847E-4	0.0	0.13867908311514454	4.2375699636619166E-4
52-53	5.527265169993804E-5	0.0010133319478321974	0.0	0.17775684786700074	4.60605430832817E-4
54-55	5.527265169993804E-5	0.0010133319478321974	0.0	0.2233844218452996	4.974538652994424E-4
56-57	5.527265169993804E-5	0.00103175616506551	0.0	0.2849857921648805	4.974538652994424E-4
58-59	5.527265169993804E-5	0.0010409682736821665	0.0	0.32656003835185055	4.974538652994424E-4
60-61	7.369686893325072E-5	0.0010501803822988228	0.0	0.3461726175967119	5.15878082532755E-4
62-63	9.21210861665634E-5	0.0010501803822988228	0.0	0.36173186905024446	5.15878082532755E-4
64-65	9.21210861665634E-5	0.0011607256856986988	0.0	0.3682356177336039	5.15878082532755E-4
66-67	1.1054530339987608E-4	0.0011791499029320115	0.0	0.37484991172036314	5.250901911494114E-4
68-69	1.1054530339987608E-4	0.0011791499029320115	0.0	0.38104966081937286	5.343022997660677E-4
70-71	1.1054530339987608E-4	0.0011791499029320115	0.0	0.38592286627758404	5.527265169993804E-4
72-73	1.2896952063318876E-4	0.0011791499029320115	0.0	0.38964455815871324	6.079991686993184E-4
74-75	1.2896952063318876E-4	0.0011791499029320115	0.0	0.39241740285232674	6.079991686993184E-4
76-77	1.2896952063318876E-4	0.0012252104460152933	0.0	0.3954113381527401	6.172112773159748E-4
78-79	1.2896952063318876E-4	0.0012344225546319495	0.0	0.39716163878990474	6.264233859326311E-4
80-81	1.2896952063318876E-4	0.0012344225546319495	0.0	0.39784333482753736	6.540597117826001E-4
82-83	1.2896952063318876E-4	0.0012344225546319495	0.0	0.39840527345315335	7.001202548658818E-4
84-85	1.2896952063318876E-4	0.0012436346632486057	0.0	0.3986079398427198	7.001202548658818E-4
86-87	1.2896952063318876E-4	0.0012896952063318875	9.21210861665634E-6	0.3987000609288864	7.093323634825381E-4
88	1.2896952063318876E-4	0.001326543640798513	1.842421723331268E-5	0.3987000609288864	7.185444720991945E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8325	0.0	51.934647	1
CTTATAC	9135	0.0	45.384552	1
TATACAC	11555	0.0	40.045517	3
GTATCAA	15135	0.0	38.18201	1
TTATACA	11740	0.0	38.124847	2
TATCAAC	17460	0.0	32.661503	2
TCAACGC	17780	0.0	32.115044	4
ATCAACG	17670	0.0	32.099533	3
CAACGCA	18235	0.0	31.313707	5
GCGCAGA	1420	0.0	31.28178	1
AACGCAG	18810	0.0	30.423943	6
TACACAT	15855	0.0	29.15818	5
GATCAAC	2715	0.0	28.359077	1
ATACACA	17270	0.0	28.018196	4
ACACATC	16390	0.0	27.509628	6
GTGGTAT	3655	0.0	27.084387	1
ACGCAGA	21415	0.0	26.782316	7
ACATCTC	17390	0.0	26.14661	8
TGGTATC	3565	0.0	25.869467	2
CGCAGAG	22160	0.0	25.824656	8
>>END_MODULE