FastQCFastQC Report
Fri 27 May 2016
SRR2140144_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140144_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5497180
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66750.049.654521
CTTATAC87800.042.347621
GTATCAA121450.037.751951
TATACAC112650.037.264713
TTATACA116300.034.7469142
TCAACGC137600.033.0397954
ATCAACG138050.032.747433
TATCAAC139850.032.4507142
CAACGCA142150.032.012135
AACGCAG146250.031.2018536
TACACAT147100.028.5375215
ACACATC145600.027.8393426
ACGCAGA167200.027.2414677
ATACACA163200.026.8417284
CGCAGAG171800.026.4873398
ACATCTC154200.026.1764778
GTGGTAT34800.025.3981
CACATCT163500.024.531627
TGGTATC32950.023.4709912
CATCTCC173100.023.367489
Produced by FastQC (version 0.11.5)