##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140144_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5497180
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.56319039944117	18.0	18.0	32.0	18.0	33.0
2	26.55843632553418	27.0	25.0	30.0	18.0	33.0
3	25.744573399452083	27.0	18.0	30.0	18.0	31.0
4	30.317975216383672	31.0	29.0	33.0	27.0	33.0
5	32.09914956395825	33.0	32.0	33.0	31.0	33.0
6	36.51510301645571	38.0	37.0	38.0	34.0	38.0
7	36.90711364736101	38.0	38.0	38.0	35.0	38.0
8	37.118866946325205	38.0	38.0	38.0	36.0	38.0
9	37.29424832368596	38.0	38.0	38.0	36.0	38.0
10-11	37.28537004427724	38.0	38.0	38.0	36.5	38.0
12-13	37.37582524130555	38.0	38.0	38.0	37.0	38.0
14-15	37.36268741427423	38.0	38.0	38.0	37.0	38.0
16-17	37.38862225723007	38.0	38.0	38.0	37.0	38.0
18-19	37.39675051571897	38.0	38.0	38.0	37.0	38.0
20-21	37.41261454707184	38.0	38.0	38.0	37.0	38.0
22-23	37.350789654308414	38.0	38.0	38.0	37.0	38.0
24-25	37.41053214227563	38.0	38.0	38.0	37.0	38.0
26-27	37.37889895552435	38.0	38.0	38.0	37.0	38.0
28-29	37.35616401084686	38.0	38.0	38.0	37.0	38.0
30-31	37.361941671733895	38.0	38.0	38.0	37.0	38.0
32-33	37.33911720013408	38.0	38.0	38.0	37.0	38.0
34-35	37.24523037459743	38.0	38.0	38.0	37.0	38.0
36-37	37.269733761947236	38.0	38.0	38.0	37.0	38.0
38-39	37.233642266279055	38.0	38.0	38.0	37.0	38.0
40-41	37.287415372149056	38.0	38.0	38.0	37.0	38.0
42-43	37.26803013666532	38.0	38.0	38.0	37.0	38.0
44-45	37.2356845154874	38.0	38.0	38.0	37.0	38.0
46-47	37.198998378009364	38.0	38.0	38.0	37.0	38.0
48-49	37.14670719204304	38.0	38.0	38.0	36.5	38.0
50-51	37.11093543891248	38.0	38.0	38.0	36.0	38.0
52-53	37.019072886698666	38.0	38.0	38.0	36.0	38.0
54-55	37.05134630772588	38.0	38.0	38.0	36.0	38.0
56-57	37.009782885310514	38.0	38.0	38.0	36.0	38.0
58-59	36.983449984658755	38.0	38.0	38.0	36.0	38.0
60-61	36.97416927353314	38.0	38.0	38.0	36.0	38.0
62-63	36.9834998181434	38.0	38.0	38.0	36.0	38.0
64-65	36.989509154009696	38.0	38.0	38.0	36.0	38.0
66-67	36.98117867343735	38.0	38.0	38.0	36.0	38.0
68-69	36.956556462047196	38.0	38.0	38.0	36.0	38.0
70-71	36.956050732692496	38.0	38.0	38.0	36.0	38.0
72-73	36.967668480556625	38.0	38.0	38.0	36.0	38.0
74-75	36.91082172975121	38.0	38.0	38.0	35.5	38.0
76-77	36.91537015217217	38.0	38.0	38.0	35.5	38.0
78-79	36.92210266162339	38.0	38.0	38.0	35.5	38.0
80-81	36.90512686803156	38.0	38.0	38.0	35.0	38.0
82-83	36.856626608652874	38.0	38.0	38.0	35.0	38.0
84-85	36.85537300962976	38.0	38.0	38.0	35.0	38.0
86-87	36.85001550368155	38.0	38.0	38.0	35.0	38.0
88-89	36.83201847695758	38.0	38.0	38.0	35.0	38.0
90-91	36.83933713340046	38.0	38.0	38.0	35.0	38.0
92-93	36.81186048207131	38.0	38.0	38.0	35.0	38.0
94-95	36.74650090110514	38.0	38.0	38.0	35.0	38.0
96-97	36.768423851910306	38.0	38.0	38.0	35.0	38.0
98-99	36.79600652088364	38.0	38.0	38.0	35.0	38.0
100	35.245404448904274	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	9.0
19	34.0
20	91.0
21	202.0
22	496.0
23	1250.0
24	2804.0
25	5517.0
26	9809.0
27	16142.0
28	24837.0
29	35379.0
30	47969.0
31	62426.0
32	82671.0
33	113968.0
34	177009.0
35	357396.0
36	1270507.0
37	3288663.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.196584430562574	13.823851502042867	10.70163247337726	22.277931594017296
2	14.49077924998308	19.08847349848522	42.2317882818862	24.188958969645498
3	19.08744119712289	25.114604215252186	30.63838186124522	25.159572726379707
4	12.685576968554788	18.12052725215474	38.65383705827352	30.540058721016962
5	13.645105308540014	37.547269691005205	35.17508977330195	13.63253522715283
6	29.840991199123913	37.4389232297287	18.844261967044922	13.875823604102466
7	26.581174347574578	32.745134778195364	22.36131980397222	18.31237107025784
8	24.209703884537163	36.78702534754183	21.294118075085773	17.709152692835236
9	25.404771173583544	17.30929312847678	20.83635609530705	36.44957960263262
10-11	24.177551035258077	26.63690292113411	28.812482036244035	20.373064007363777
12-13	25.255039856799304	23.96151299393507	28.72868452552036	22.054762623745265
14-15	22.429836752662276	25.45516974157659	26.00211381108132	26.11287969467982
16-17	22.248507416529932	28.941202580232044	26.633382934522793	22.17690706871523
18-19	22.29904241811256	27.70901625924565	28.338302547851807	21.653638774789982
20-21	22.857005902361273	26.90068548596884	28.27706025080033	21.96524836086956
22-23	22.51781973071679	27.285129244496904	28.15261401075674	22.044437014029565
24-25	22.581032795176604	27.54783918610617	28.21383997933245	21.657288039384774
26-27	22.500348212190822	27.825015254468738	28.18816577101912	21.48647076232132
28-29	22.346832443628003	27.56723395374394	28.2363446667876	21.849588935840462
30-31	22.975128049353344	27.26766659173258	28.17592421566838	21.581281143245697
32-33	22.415980828967417	27.49016144734063	28.374587714205262	21.719270009486692
34-35	22.4491321430662	27.1045428733518	28.51328916664903	21.933035816932968
36-37	22.171875056063254	27.57438093057228	28.418317483206785	21.835426530157683
38-39	22.04606005268481	27.011628313836177	28.825494204726525	22.11681742875249
40-41	22.365449439772515	26.81768802046745	28.82935469023976	21.98750784952027
42-43	22.5740497940666	26.798781514224494	28.674393596263254	21.952775095445645
44-45	22.172859290447487	27.221629568876978	28.63267471646341	21.97283642421213
46-47	22.731195377955647	26.762246378973213	28.005990465918458	22.500567777152686
48-49	22.480004198310986	26.854023342916655	28.16000755080841	22.50596490796395
50-51	22.1617360571354	27.107510186453837	28.44660296695878	22.28415078945199
52-53	22.35278071042485	27.149427043529784	28.662019246295227	21.835772999750137
54-55	22.31742696842936	27.10945814449585	28.22048476016657	22.35263012690822
56-57	22.63432986794544	27.64442579284772	27.62183856838389	22.09940577082295
58-59	22.052095940680164	28.07434067912673	28.29836147046053	21.57520190973257
60-61	22.48159332099426	28.617260373403774	27.40547748135926	21.495668824242706
62-63	21.998658911248377	29.06457993283995	27.385578655017767	21.551182500893905
64-65	21.973633234873905	28.95970884433503	27.447335138536722	21.619322782254343
66-67	22.138898540914447	28.852747281006252	27.31647317163762	21.691881006441676
68-69	22.203863791954255	28.489116476940325	27.52264332827149	21.78437640283393
70-71	22.262007744702	28.0289911296926	27.687634542440392	22.021366583165005
72-73	22.574602496308476	27.63210764108451	27.717053753934813	22.076236108672205
74-75	22.300259354459424	27.702540376984658	27.810109006152096	22.18709126240382
76-77	22.50917675346586	27.458029649642313	27.75215290266576	22.28064069422607
78-79	22.49389327085348	27.605308740180632	27.624224495747683	22.2765734932182
80-81	22.520735430231092	27.59654033677131	27.592583558281436	22.290140674716163
82-83	22.481281309019383	27.49147395282651	27.752473774526226	22.274770963627876
84-85	22.580993503186964	27.511493858898767	27.660335691982695	22.247176945931578
86-87	22.448848254519135	27.539779610926	27.7354844974946	22.275887637060265
88-89	22.52556282891175	27.534831934178243	27.6683669185902	22.27123831831981
90-91	22.558135989411802	27.574494205930698	27.630158115047493	22.237211689610007
92-93	22.624136706753387	27.551568227463584	27.635036433567333	22.189258632215694
94-95	22.51770914279909	27.6297054812003	27.61168256818296	22.240902807817644
96-97	22.58398118641761	27.675137548488504	27.736571557673663	22.004309707420227
98-99	23.586169226945316	29.611338115893776	27.93413321057337	18.868359446587537
100	22.52081004269763	30.47456213604874	21.90725612740146	25.09737169385217
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	535.0
1	599.5
2	968.5
3	1410.5
4	1784.0
5	2498.5
6	3158.0
7	3759.0
8	5009.0
9	6026.5
10	5971.0
11	5814.5
12	6344.5
13	7705.5
14	10159.5
15	13012.0
16	15979.5
17	18805.5
18	19643.5
19	18461.5
20	16808.0
21	15473.5
22	15404.0
23	17737.5
24	22301.5
25	28970.5
26	38308.0
27	48704.5
28	58800.0
29	70436.0
30	81945.5
31	92996.5
32	105310.0
33	117566.5
34	130599.0
35	143404.0
36	157060.5
37	169367.0
38	179139.5
39	188222.5
40	198073.5
41	207876.0
42	216537.0
43	234790.0
44	256648.0
45	265351.0
46	260727.5
47	252070.0
48	242342.0
49	233265.0
50	228098.5
51	221986.0
52	213414.5
53	203105.5
54	192239.0
55	179790.0
56	165050.0
57	149015.5
58	132219.5
59	115309.5
60	96645.0
61	78061.5
62	61921.0
63	48003.0
64	36355.5
65	27144.5
66	19786.5
67	14807.5
68	11309.5
69	8312.5
70	6054.0
71	4350.0
72	3096.0
73	2131.5
74	1406.5
75	910.0
76	597.5
77	369.5
78	243.0
79	176.5
80	113.5
81	68.5
82	43.0
83	28.5
84	20.0
85	13.5
86	9.5
87	7.0
88	4.0
89	2.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012078920464674615
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.9109136703055514E-4
26-27	2.092452940733363E-4
28-29	0.003540431914490833
30-31	0.0058953582118057325
32-33	0.004064125878673148
34-35	0.0019145186652745336
36-37	0.0
38-39	0.0
40-41	3.35408643257131E-4
42-43	0.0
44-45	9.448856693064568E-6
46-47	4.669903576974592E-4
48-49	0.0013936506621426523
50-51	0.0027706270519956603
52-53	0.0
54-55	0.0025642726087052635
56-57	0.03887135097296169
58-59	0.06279697987832711
60-61	0.0424355105021096
62-63	0.014113662502527005
64-65	2.1523006915752084E-4
66-67	0.0
68-69	0.0
70-71	0.002769464354703621
72-73	0.0047574480010933486
74-75	0.004223977786461005
76-77	0.0028975192607910557
78-79	0.007866110127321949
80-81	0.015172842275719542
82-83	0.043375152156423895
84-85	0.019644546036775873
86-87	0.0018403421045504137
88-89	0.0010523662103450874
90-91	8.272760965426477E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	33.0
20-21	149.0
22-23	367.0
24-25	448.0
26-27	1602.0
28-29	4345.0
30-31	11974.0
32-33	15398.0
34-35	20760.0
36-37	31808.0
38-39	35169.0
40-41	34446.0
42-43	38264.0
44-45	44522.0
46-47	45022.0
48-49	41859.0
50-51	37636.0
52-53	34930.0
54-55	38123.0
56-57	38611.0
58-59	41580.0
60-61	45027.0
62-63	44740.0
64-65	54329.0
66-67	59939.0
68-69	60213.0
70-71	79875.0
72-73	44683.0
74-75	42131.0
76-77	43876.0
78-79	45355.0
80-81	44715.0
82-83	42247.0
84-85	42358.0
86-87	43452.0
88-89	44999.0
90-91	45634.0
92-93	45193.0
94-95	53194.0
96-97	168130.0
98-99	366380.0
100-101	3563664.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.86752442562984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.54205639869049	80.4357387658432
2	6.083034137961276	10.690023013985051
3	1.3706382410370137	3.613037673690663
4	0.4894004153821739	1.720096118100262
5	0.1976401868278821	0.8683076971792486
6	0.10394370398972783	0.547996556952471
7	0.06477395161715573	0.3984068743105498
8	0.03915566418000858	0.2752409022990931
9	0.026199491592057287	0.20718760206637596
>10	0.0813573961762531	1.067632335920843
>50	0.001337811753325901	0.07981649404469178
>100	4.0272276270496754E-4	0.058923733022233735
>500	5.987802997654543E-5	0.037592232585291215
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.819114527812442E-5
2	0.0	1.819114527812442E-5	0.0	0.0	1.819114527812442E-5
3	0.0	1.819114527812442E-5	0.0	0.0	1.819114527812442E-5
4	0.0	3.638229055624884E-5	0.0	0.0	1.819114527812442E-5
5	0.0	3.638229055624884E-5	0.0	0.0	1.819114527812442E-5
6	0.0	3.638229055624884E-5	0.0	0.0	3.638229055624884E-5
7	0.0	3.638229055624884E-5	0.0	0.0	3.638229055624884E-5
8	0.0	3.638229055624884E-5	0.0	0.0	3.638229055624884E-5
9	1.819114527812442E-5	1.0914687166874652E-4	0.0	0.0	3.638229055624884E-5
10-11	1.819114527812442E-5	1.0914687166874652E-4	0.0	3.638229055624884E-5	3.638229055624884E-5
12-13	3.638229055624884E-5	1.0914687166874652E-4	0.0	7.276458111249768E-5	3.638229055624884E-5
14-15	3.638229055624884E-5	1.637203075031198E-4	0.0	7.276458111249768E-5	5.457343583437326E-5
16-17	3.638229055624884E-5	1.72815880142182E-4	0.0	7.276458111249768E-5	5.457343583437326E-5
18-19	3.638229055624884E-5	1.910070254203064E-4	0.0	8.186015375155988E-5	5.457343583437326E-5
20-21	3.638229055624884E-5	2.0010259805936862E-4	0.0	9.09557263906221E-5	5.457343583437326E-5
22-23	3.638229055624884E-5	2.1829374333749303E-4	0.0	1.0005129902968431E-4	6.366900847343547E-5
24-25	3.638229055624884E-5	2.2738931597655524E-4	0.0	1.910070254203064E-4	7.276458111249768E-5
26-27	3.638229055624884E-5	2.546760338937419E-4	0.0	2.3648488861561747E-4	7.276458111249768E-5
28-29	3.638229055624884E-5	2.546760338937419E-4	0.0	8.276971101546611E-4	7.276458111249768E-5
30-31	3.638229055624884E-5	3.092494697281151E-4	0.0	0.0025740470568546055	7.276458111249768E-5
32-33	3.638229055624884E-5	3.092494697281151E-4	0.0	0.005948504505946686	7.276458111249768E-5
34-35	3.638229055624884E-5	3.6382290556248836E-4	0.0	0.010769158004649657	7.276458111249768E-5
36-37	3.638229055624884E-5	4.1839634139686165E-4	0.0	0.018163858560207233	7.276458111249768E-5
38-39	3.638229055624884E-5	4.1839634139686165E-4	0.0	0.03676430460708945	7.276458111249768E-5
40-41	3.638229055624884E-5	4.5477863195311053E-4	0.0	0.05629249906315602	7.276458111249768E-5
42-43	3.638229055624884E-5	4.5477863195311053E-4	0.0	0.06748005340920255	7.276458111249768E-5
44-45	3.638229055624884E-5	5.002564951484215E-4	0.0	0.0806049647273693	7.276458111249768E-5
46-47	3.638229055624884E-5	5.639255036218571E-4	0.0	0.09458485987360793	7.276458111249768E-5
48-49	3.638229055624884E-5	5.639255036218571E-4	0.0	0.1088649089169356	7.276458111249768E-5
50-51	3.638229055624884E-5	6.548812300124791E-4	0.0	0.12232635642274767	7.276458111249768E-5
52-53	3.638229055624884E-5	6.730723752906035E-4	0.0	0.15877231598746994	8.186015375155988E-5
54-55	3.638229055624884E-5	6.730723752906035E-4	0.0	0.2051870231646044	9.09557263906221E-5
56-57	3.638229055624884E-5	6.730723752906035E-4	0.0	0.2693562881331883	1.0005129902968431E-4
58-59	3.638229055624884E-5	6.730723752906035E-4	0.0	0.31245111129706504	1.0914687166874652E-4
60-61	3.638229055624884E-5	6.730723752906035E-4	0.0	0.3286048483040396	1.0914687166874652E-4
62-63	3.638229055624884E-5	6.730723752906035E-4	0.0	0.33969235135105635	1.0914687166874652E-4
64-65	3.638229055624884E-5	8.186015375155989E-4	0.0	0.3455589957032515	1.0914687166874652E-4
66-67	3.638229055624884E-5	8.458882554327855E-4	0.0	0.3506343252358482	1.0914687166874652E-4
68-69	3.638229055624884E-5	8.6407940071091E-4	0.0	0.35571875034108397	1.0914687166874652E-4
70-71	3.638229055624884E-5	8.731749733499721E-4	0.0	0.3605303082671479	1.0914687166874652E-4
72-73	3.638229055624884E-5	8.731749733499721E-4	0.0	0.36384109670776654	1.0914687166874652E-4
74-75	3.638229055624884E-5	9.186528365452832E-4	0.0	0.366542481781568	1.0914687166874652E-4
76-77	3.638229055624884E-5	9.641306997405942E-4	0.0	0.3694985428892632	1.0914687166874652E-4
78-79	3.638229055624884E-5	9.823218450187186E-4	0.0	0.37159052459624753	1.0914687166874652E-4
80-81	3.638229055624884E-5	9.823218450187186E-4	0.0	0.3722545013988991	1.0914687166874652E-4
82-83	3.638229055624884E-5	9.823218450187186E-4	0.0	0.37271837560349125	1.0914687166874652E-4
84-85	3.638229055624884E-5	9.823218450187186E-4	0.0	0.37292757377418967	1.0914687166874652E-4
86-87	3.638229055624884E-5	9.823218450187186E-4	0.0	0.37300033835530216	1.0914687166874652E-4
88	3.638229055624884E-5	0.001036895280853092	0.0	0.3730094339279412	1.0914687166874652E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6675	0.0	49.65452	1
CTTATAC	8780	0.0	42.34762	1
GTATCAA	12145	0.0	37.75195	1
TATACAC	11265	0.0	37.26471	3
TTATACA	11630	0.0	34.746914	2
TCAACGC	13760	0.0	33.039795	4
ATCAACG	13805	0.0	32.74743	3
TATCAAC	13985	0.0	32.450714	2
CAACGCA	14215	0.0	32.01213	5
AACGCAG	14625	0.0	31.201853	6
TACACAT	14710	0.0	28.537521	5
ACACATC	14560	0.0	27.839342	6
ACGCAGA	16720	0.0	27.241467	7
ATACACA	16320	0.0	26.841728	4
CGCAGAG	17180	0.0	26.487339	8
ACATCTC	15420	0.0	26.176477	8
GTGGTAT	3480	0.0	25.398	1
CACATCT	16350	0.0	24.53162	7
TGGTATC	3295	0.0	23.470991	2
CATCTCC	17310	0.0	23.36748	9
>>END_MODULE