FastQCFastQC Report
Fri 27 May 2016
SRR2140143_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140143_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5256793
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61850.048.996431
GCTCCGA16200.041.1012841
CTTATAC96050.036.8830221
GTATCAA114100.036.060951
ATCAACG124400.032.282373
TCAACGC125200.032.2465324
TATACAC125000.031.820143
TATCAAC127900.031.5694032
CAACGCA129050.031.317585
TTATACA127300.030.578222
AACGCAG133300.030.3190846
ACGCAGA152250.026.5453767
CGCAGAG158500.025.4986348
ACACATC154250.024.7624766
TACACAT162050.024.7293575
ACATCTC159600.023.9324058
GATCAAC23350.023.7630541
GTGGTAT33300.022.8150581
ATACACA186400.022.8039264
TGGTATC30800.022.7268332
Produced by FastQC (version 0.11.5)