##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140143_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5256793
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.565837574353793	18.0	18.0	32.0	18.0	33.0
2	26.680613256028913	27.0	25.0	30.0	18.0	33.0
3	25.889493270897294	27.0	18.0	30.0	18.0	31.0
4	30.362342211306398	31.0	29.0	33.0	27.0	33.0
5	32.090676387675906	33.0	32.0	33.0	31.0	33.0
6	36.51234526449872	38.0	37.0	38.0	34.0	38.0
7	36.8784993055652	38.0	38.0	38.0	35.0	38.0
8	37.101714676609866	38.0	38.0	38.0	36.0	38.0
9	37.27888143969146	38.0	38.0	38.0	36.0	38.0
10-11	37.272342662151615	38.0	38.0	38.0	36.5	38.0
12-13	37.36712545082145	38.0	38.0	38.0	37.0	38.0
14-15	37.35267120086334	38.0	38.0	38.0	37.0	38.0
16-17	37.37538590543702	38.0	38.0	38.0	37.0	38.0
18-19	37.38551147058672	38.0	38.0	38.0	37.0	38.0
20-21	37.40010414934628	38.0	38.0	38.0	37.0	38.0
22-23	37.33598248604591	38.0	38.0	38.0	37.0	38.0
24-25	37.40012348261146	38.0	38.0	38.0	37.0	38.0
26-27	37.36591172797018	38.0	38.0	38.0	37.0	38.0
28-29	37.32685114038013	38.0	38.0	38.0	37.0	38.0
30-31	37.34061384531239	38.0	38.0	38.0	37.0	38.0
32-33	37.323473403648585	38.0	38.0	38.0	37.0	38.0
34-35	37.2335925462858	38.0	38.0	38.0	37.0	38.0
36-37	37.25379705557963	38.0	38.0	38.0	37.0	38.0
38-39	37.21016531111587	38.0	38.0	38.0	37.0	38.0
40-41	37.26994101500795	38.0	38.0	38.0	37.0	38.0
42-43	37.254412528211304	38.0	38.0	38.0	37.0	38.0
44-45	37.22015241526164	38.0	38.0	38.0	37.0	38.0
46-47	37.186659497589616	38.0	38.0	38.0	37.0	38.0
48-49	37.13392565899872	38.0	38.0	38.0	36.0	38.0
50-51	37.098254878848095	38.0	38.0	38.0	36.0	38.0
52-53	37.003169052044285	38.0	38.0	38.0	36.0	38.0
54-55	37.03766310210614	38.0	38.0	38.0	36.0	38.0
56-57	36.99526641636923	38.0	38.0	38.0	36.0	38.0
58-59	36.9671093125974	38.0	38.0	38.0	36.0	38.0
60-61	36.95798808274653	38.0	38.0	38.0	36.0	38.0
62-63	36.96972936620418	38.0	38.0	38.0	36.0	38.0
64-65	36.979271678051816	38.0	38.0	38.0	36.0	38.0
66-67	36.97302161003976	38.0	38.0	38.0	36.0	38.0
68-69	36.953641587309335	38.0	38.0	38.0	36.0	38.0
70-71	36.95494826557268	38.0	38.0	38.0	36.0	38.0
72-73	36.97242437779783	38.0	38.0	38.0	36.0	38.0
74-75	36.91739524261357	38.0	38.0	38.0	35.5	38.0
76-77	36.921273784165265	38.0	38.0	38.0	35.5	38.0
78-79	36.92243016018534	38.0	38.0	38.0	35.5	38.0
80-81	36.899646923881264	38.0	38.0	38.0	35.0	38.0
82-83	36.85081471093518	38.0	38.0	38.0	35.0	38.0
84-85	36.84956645449326	38.0	38.0	38.0	35.0	38.0
86-87	36.84179668003013	38.0	38.0	38.0	35.0	38.0
88-89	36.81880046764969	38.0	38.0	38.0	35.0	38.0
90-91	36.829856750855996	38.0	38.0	38.0	35.0	38.0
92-93	36.803475435715555	38.0	38.0	38.0	35.0	38.0
94-95	36.73053405913737	38.0	38.0	38.0	35.0	38.0
96-97	36.75545321609394	38.0	38.0	38.0	35.0	38.0
98-99	36.78033418307854	38.0	38.0	38.0	35.0	38.0
100	35.232205377346894	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	23.0
20	88.0
21	217.0
22	474.0
23	1232.0
24	2724.0
25	5425.0
26	10008.0
27	15682.0
28	24360.0
29	34971.0
30	47011.0
31	61579.0
32	80475.0
33	110945.0
34	172248.0
35	345118.0
36	1181824.0
37	3162389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.50438813169931	14.37033948264655	10.86011946827657	22.265152917377574
2	14.324050767776047	19.341050096174154	42.36186975489385	23.973029381155957
3	19.06936415415254	25.18533638284787	30.669839957555872	25.07545950544372
4	12.536654952934231	18.160235717860683	38.90904587644977	30.394063452755322
5	13.517081612306212	37.58489253809309	35.217080071442794	13.680945778157898
6	29.650149815676592	37.94889774050452	18.71523189138321	13.685720552435676
7	26.327858068598097	33.006816132954064	22.421959548340595	18.243366250107243
8	24.080366109146777	37.21137583313629	21.162845864389183	17.545412193327756
9	25.096175557987543	17.594130109365157	20.91339719863422	36.396297134013075
10-11	23.899010290114145	26.963702013756297	28.8137558393492	20.32353185678036
12-13	24.954901210681115	24.299149690695447	28.789568088376317	21.95638101024712
14-15	22.184609513823354	26.00673071966121	26.021911077723626	25.786748688791818
16-17	21.978495253665116	29.47254152864684	26.705654949700318	21.843308267987727
18-19	22.011319448949198	27.97981583067852	28.435626055657888	21.5732386647144
20-21	22.5914874836638	27.313957536339128	28.374807151974707	21.719747828022363
22-23	22.42786681254151	27.54938066922461	28.240478898656363	21.782273619577513
24-25	22.39770047108114	27.50659363301331	28.524561532679364	21.571144363226182
26-27	22.436648217574533	27.77174245502469	28.35881725771023	21.43279206969055
28-29	21.933620148220793	27.397214381073454	28.51562302956735	22.153542441138402
30-31	22.589415507406045	27.392152627467375	28.594678188400625	21.42375367672595
32-33	22.685725994754886	27.30278730546849	28.73298168563643	21.278505014140194
34-35	21.81693341451642	27.540743227399233	29.083924053917492	21.55839930416685
36-37	22.351585003326527	27.529323426822188	28.547461648803413	21.571629921047876
38-39	21.848106967420108	27.42471776080358	28.87735672351984	21.849818548256476
40-41	22.00588352442902	27.220859241294455	28.988776900361167	21.784480333915354
42-43	22.237907246229828	27.22373149788531	28.970734257851287	21.567626998033575
44-45	21.884480888303955	27.63937577510961	28.793819513367723	21.68232382321871
46-47	22.45276119874833	27.209149874558747	28.15902586487657	22.17906306181635
48-49	22.18401680637401	27.2972384359713	28.391715358025937	22.12702939962875
50-51	21.887371316096985	27.70418465289326	28.51379205464878	21.89465197636098
52-53	22.032596977966264	27.816829929407266	28.681904748351066	21.4686683442754
54-55	21.984437888005225	27.844394433773434	28.278322789035975	21.892844889185366
56-57	22.307241530900164	28.30454283397818	27.67649989236554	21.71171574275612
58-59	21.67978179785636	28.631645078760293	28.292986504904505	21.395586618478838
60-61	22.239726360141688	29.027827330658436	27.64386282276773	21.088583486432142
62-63	21.623425630921105	29.403594896198836	27.62159975570612	21.35137971717393
64-65	21.614792256652592	29.313188523887813	27.647782327059723	21.424236892399872
66-67	21.792337205899965	29.232456123831163	27.693530851198805	21.281675819070063
68-69	21.81608639931533	29.11428631190156	27.69226562455743	21.377361664225674
70-71	21.854679965374377	28.64589931905223	27.680031316373466	21.819389399199927
72-73	22.13031966937035	28.24083444625616	27.735507930212204	21.893337954161282
74-75	21.91918498988685	28.313021331246922	27.98023728860681	21.787556390259414
76-77	22.13295559736322	27.825195565357213	27.9711448818034	22.07070395547617
78-79	22.092239104491696	28.010918812049823	27.67163928332637	22.225202800132113
80-81	22.317404029712186	28.13202007303723	27.64403240780009	21.9065434894505
82-83	22.35828567325656	27.823504649937185	27.8123238704597	22.005885806346555
84-85	22.290730472722313	27.746208355347502	27.759334007487656	22.203727164442526
86-87	22.136099699429014	27.983243785415073	27.80386133824011	22.0767951769158
88-89	22.363008954063478	28.038766979604517	27.757174759719987	21.841049306612014
90-91	22.444349495472615	27.960631687822723	27.790953706935884	21.804065109768782
92-93	22.206269224555108	27.77027092710146	27.763675344246973	22.259784504096462
94-95	22.29853068183302	28.10901220359175	27.753194839012945	21.839262275562287
96-97	22.6125073182138	27.91418312446727	27.941517923484533	21.531791633834395
98-99	23.28284340564742	30.044375330049306	28.18342095171125	18.489360312592034
100	22.200099767931118	30.915132192508732	22.243737393454357	24.641030646105797
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	498.0
1	537.5
2	775.0
3	1076.5
4	1446.5
5	2105.5
6	2685.5
7	3258.0
8	4215.5
9	4951.0
10	5001.0
11	5172.5
12	5932.0
13	7247.5
14	9498.0
15	12241.5
16	15126.5
17	17953.5
18	19003.0
19	18038.0
20	16528.5
21	15396.0
22	15692.5
23	18068.5
24	22868.5
25	29840.5
26	39574.0
27	50843.0
28	61135.5
29	71832.5
30	83022.0
31	94006.0
32	105536.0
33	116983.0
34	129693.5
35	142531.0
36	156119.0
37	168698.5
38	177565.0
39	185323.5
40	194906.0
41	204009.0
42	210356.5
43	216018.5
44	219834.0
45	225520.0
46	247460.0
47	264351.0
48	260082.0
49	250217.5
50	237015.5
51	222139.0
52	205107.0
53	187460.5
54	171527.5
55	156540.0
56	141790.0
57	126625.0
58	111340.5
59	96620.5
60	80899.5
61	65284.0
62	51316.0
63	39344.5
64	29568.0
65	21843.0
66	16069.5
67	12273.5
68	9355.5
69	6782.0
70	4921.5
71	3559.5
72	2465.0
73	1644.0
74	1090.5
75	755.0
76	535.0
77	338.0
78	203.5
79	136.0
80	97.5
81	67.0
82	40.5
83	25.0
84	16.5
85	14.5
86	11.5
87	6.0
88	3.0
89	2.5
90	2.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013373172578794713
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.329385949934216E-4
26-27	2.473530135636025E-4
28-29	0.0036450931716269755
30-31	0.006383334884706015
32-33	0.004449346576218261
34-35	0.0021638992852334267
36-37	0.0
38-39	0.0
40-41	3.0159855014766655E-4
42-43	0.0
44-45	9.859876367010234E-6
46-47	3.181254774367517E-4
48-49	0.001753890479862431
50-51	0.0030596013369751
52-53	0.0
54-55	0.0028855413198772967
56-57	0.039955178472475504
58-59	0.06447418497798144
60-61	0.043995781918767476
62-63	0.014849512112318842
64-65	4.577054607880538E-4
66-67	0.0
68-69	0.0
70-71	0.0027723588532463437
72-73	0.004973041968330011
74-75	0.004656002236689229
76-77	0.003511507769609975
78-79	0.00867629635834899
80-81	0.016914447052088364
82-83	0.044175645744860374
84-85	0.019856150590560732
86-87	0.0019704094654171642
88-89	0.0012057413060324563
90-91	6.0899388972070686E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	38.0
20-21	123.0
22-23	331.0
24-25	440.0
26-27	1440.0
28-29	3803.0
30-31	10796.0
32-33	13639.0
34-35	18882.0
36-37	28877.0
38-39	31455.0
40-41	31334.0
42-43	34692.0
44-45	40912.0
46-47	41315.0
48-49	38170.0
50-51	34112.0
52-53	31635.0
54-55	34371.0
56-57	36540.0
58-59	22880.0
60-61	49169.0
62-63	43380.0
64-65	49721.0
66-67	55319.0
68-69	55864.0
70-71	72579.0
72-73	41061.0
74-75	38456.0
76-77	40201.0
78-79	40841.0
80-81	41124.0
82-83	38422.0
84-85	38238.0
86-87	39604.0
88-89	41285.0
90-91	42086.0
92-93	41607.0
94-95	49376.0
96-97	175462.0
98-99	349188.0
100-101	3458025.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.11592178938605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.06406769185196	82.04374256039823
2	5.907018720475472	10.528188366046628
3	1.2402169176913271	3.315692215165613
4	0.37770669673412044	1.3463872178194094
5	0.17126581375302316	0.7631255431804986
6	0.09043660080729249	0.4835604626664363
7	0.04580430585815175	0.28573250569305186
8	0.025334216926667628	0.1806145675385802
9	0.021022269352118164	0.1686077020276893
>10	0.055480840964370115	0.7053478127460607
>50	0.0010790215696921935	0.06161799999997408
>100	5.08566748451182E-4	0.07545489146257904
>500	5.833726730950842E-5	0.04192815525527057
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.902300509074639E-5	0.0
4	0.0	1.902300509074639E-5	0.0	1.902300509074639E-5	0.0
5	0.0	1.902300509074639E-5	0.0	1.902300509074639E-5	0.0
6	0.0	1.902300509074639E-5	0.0	1.902300509074639E-5	0.0
7	0.0	3.804601018149278E-5	0.0	1.902300509074639E-5	0.0
8	0.0	5.7069015272239174E-5	0.0	1.902300509074639E-5	0.0
9	0.0	9.511502545373196E-5	0.0	1.902300509074639E-5	1.902300509074639E-5
10-11	0.0	9.511502545373196E-5	0.0	1.902300509074639E-5	1.902300509074639E-5
12-13	0.0	9.511502545373196E-5	0.0	1.902300509074639E-5	1.902300509074639E-5
14-15	1.902300509074639E-5	1.4267253818059795E-4	0.0	1.902300509074639E-5	3.804601018149278E-5
16-17	2.8534507636119587E-5	1.5218404072597113E-4	0.0	1.902300509074639E-5	7.609202036298557E-5
18-19	3.804601018149278E-5	1.5218404072597113E-4	0.0	1.902300509074639E-5	7.609202036298557E-5
20-21	3.804601018149278E-5	1.5218404072597113E-4	0.0	4.7557512726865975E-5	7.609202036298557E-5
22-23	3.804601018149278E-5	1.9974155345283713E-4	0.0	5.7069015272239174E-5	7.609202036298557E-5
24-25	3.804601018149278E-5	2.092530559982103E-4	0.0	7.609202036298557E-5	7.609202036298557E-5
26-27	3.804601018149278E-5	2.187645585435835E-4	0.0	1.3316103563522475E-4	7.609202036298557E-5
28-29	3.804601018149278E-5	2.472990661797031E-4	0.0	7.989662138113485E-4	7.609202036298557E-5
30-31	3.804601018149278E-5	2.663220712704495E-4	0.0	0.0019023005090746392	7.609202036298557E-5
32-33	3.804601018149278E-5	2.853450763611959E-4	0.0	0.004812820287958838	7.609202036298557E-5
34-35	3.804601018149278E-5	3.2339108654268867E-4	0.0	0.00833207622974692	7.609202036298557E-5
36-37	3.804601018149278E-5	3.5192559417880826E-4	0.0	0.014048489259516211	7.609202036298557E-5
38-39	4.7557512726865975E-5	3.6143709672418144E-4	0.0	0.030779222236827664	7.609202036298557E-5
40-41	5.7069015272239174E-5	3.9948310690567426E-4	0.0	0.04905081862648957	7.609202036298557E-5
42-43	5.7069015272239174E-5	4.0899460945104744E-4	0.0	0.05824844158786545	7.609202036298557E-5
44-45	5.7069015272239174E-5	4.280176145417938E-4	0.0	0.0690154624692279	7.609202036298557E-5
46-47	5.7069015272239174E-5	4.3752911708716703E-4	0.0	0.08058144956440172	9.511502545373195E-5
48-49	5.7069015272239174E-5	4.3752911708716703E-4	0.0	0.09118677490249283	1.1413803054447835E-4
50-51	5.7069015272239174E-5	4.3752911708716703E-4	0.0	0.1017350312253117	1.1413803054447835E-4
52-53	5.7069015272239174E-5	4.3752911708716703E-4	0.0	0.13541526173847818	1.1413803054447835E-4
54-55	5.7069015272239174E-5	4.470406196325402E-4	0.0	0.1790255009090143	1.3316103563522475E-4
56-57	5.7069015272239174E-5	4.565521221779134E-4	0.0	0.23308888137691552	1.6169554327134434E-4
58-59	5.7069015272239174E-5	5.041096349047793E-4	0.0	0.42856928168942543	1.7120704581671754E-4
60-61	5.7069015272239174E-5	5.516671476316454E-4	0.0	0.6009652653243146	1.7120704581671754E-4
62-63	5.7069015272239174E-5	5.706901527223918E-4	0.0	0.6078325701620741	1.7120704581671754E-4
64-65	5.7069015272239174E-5	5.992246603585113E-4	0.0	0.6134443566638443	1.7120704581671754E-4
66-67	5.7069015272239174E-5	6.182476654492577E-4	0.0	0.619018097155433	1.8071854836209072E-4
68-69	5.7069015272239174E-5	6.372706705400042E-4	0.0	0.6248962057284736	1.9974155345283713E-4
70-71	5.7069015272239174E-5	6.562936756307505E-4	0.0	0.6296424454986149	2.282760610889567E-4
72-73	5.7069015272239174E-5	6.658051781761237E-4	0.0	0.6332092589531297	2.282760610889567E-4
74-75	5.7069015272239174E-5	6.75316680721497E-4	0.0	0.6360627097167417	2.282760610889567E-4
76-77	5.7069015272239174E-5	6.848281832668702E-4	0.0	0.6389351834854444	2.282760610889567E-4
78-79	5.7069015272239174E-5	6.848281832668702E-4	0.0	0.6406757884512477	2.282760610889567E-4
80-81	5.7069015272239174E-5	6.848281832668702E-4	0.0	0.6413701281370601	2.377875636343299E-4
82-83	5.7069015272239174E-5	6.943396858122433E-4	0.0	0.6417981457516018	3.0436808145194226E-4
84-85	5.7069015272239174E-5	7.228741934483629E-4	0.0	0.6419503297923277	3.0436808145194226E-4
86-87	7.609202036298557E-5	7.418971985391093E-4	0.0	0.6420644678228722	3.0436808145194226E-4
88	7.609202036298557E-5	8.179892189020949E-4	0.0	0.6420644678228722	3.0436808145194226E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6185	0.0	48.99643	1
GCTCCGA	1620	0.0	41.101284	1
CTTATAC	9605	0.0	36.883022	1
GTATCAA	11410	0.0	36.06095	1
ATCAACG	12440	0.0	32.28237	3
TCAACGC	12520	0.0	32.246532	4
TATACAC	12500	0.0	31.82014	3
TATCAAC	12790	0.0	31.569403	2
CAACGCA	12905	0.0	31.31758	5
TTATACA	12730	0.0	30.57822	2
AACGCAG	13330	0.0	30.319084	6
ACGCAGA	15225	0.0	26.545376	7
CGCAGAG	15850	0.0	25.498634	8
ACACATC	15425	0.0	24.762476	6
TACACAT	16205	0.0	24.729357	5
ACATCTC	15960	0.0	23.932405	8
GATCAAC	2335	0.0	23.763054	1
GTGGTAT	3330	0.0	22.815058	1
ATACACA	18640	0.0	22.803926	4
TGGTATC	3080	0.0	22.726833	2
>>END_MODULE