FastQCFastQC Report
Fri 27 May 2016
SRR2140142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5201821
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65600.048.3866651
GTATCAA119050.038.549011
TCAACGC132300.033.8273934
ATCAACG132050.033.726593
TATCAAC134450.033.2915732
CAACGCA135900.032.8992655
AACGCAG140950.031.8131986
ACGCAGA162600.027.5237627
CGCAGAG167900.026.6549368
GTGGTAT33800.026.4082221
GCGCAGA12850.025.7520891
TGGTATC32600.024.3083692
GTCAACG6700.024.0452291
GCAGAGT200700.022.277089
GAGTACT111700.021.04630312-13
GTACATG158550.021.0086021
TACATGG153850.020.8296662
ACATGGG156200.019.9835833
CAGAGTA192800.019.72382410-11
CATGGGG109000.018.6120684
Produced by FastQC (version 0.11.5)