##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140142_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5201821
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.630876379637055	18.0	18.0	32.0	18.0	33.0
2	26.416134465219006	27.0	25.0	30.0	18.0	33.0
3	25.704768964560678	27.0	18.0	30.0	18.0	31.0
4	30.289764296003266	31.0	29.0	33.0	27.0	33.0
5	32.08635783507353	33.0	32.0	33.0	31.0	33.0
6	36.48036658700867	38.0	37.0	38.0	34.0	38.0
7	36.90308182461488	38.0	38.0	38.0	35.0	38.0
8	37.08999233153159	38.0	38.0	38.0	36.0	38.0
9	37.27066137031628	38.0	38.0	38.0	36.0	38.0
10-11	37.25825523408053	38.0	38.0	38.0	36.5	38.0
12-13	37.343815271613536	38.0	38.0	38.0	37.0	38.0
14-15	37.331179984855304	38.0	38.0	38.0	37.0	38.0
16-17	37.35446702222164	38.0	38.0	38.0	37.0	38.0
18-19	37.365408671309524	38.0	38.0	38.0	37.0	38.0
20-21	37.38237468475887	38.0	38.0	38.0	37.0	38.0
22-23	37.31918626680775	38.0	38.0	38.0	37.0	38.0
24-25	37.37703342342566	38.0	38.0	38.0	37.0	38.0
26-27	37.34979102707513	38.0	38.0	38.0	37.0	38.0
28-29	37.32875042360518	38.0	38.0	38.0	37.0	38.0
30-31	37.33438006503721	38.0	38.0	38.0	37.0	38.0
32-33	37.313963138585784	38.0	38.0	38.0	37.0	38.0
34-35	37.22664456212728	38.0	38.0	38.0	37.0	38.0
36-37	37.25178976736069	38.0	38.0	38.0	37.0	38.0
38-39	37.231961111559656	38.0	38.0	38.0	37.0	38.0
40-41	37.256549657493096	38.0	38.0	38.0	37.0	38.0
42-43	37.230002402789	38.0	38.0	38.0	37.0	38.0
44-45	37.19356240044392	38.0	38.0	38.0	36.5	38.0
46-47	37.15376249640736	38.0	38.0	38.0	36.0	38.0
48-49	37.09822112607036	38.0	38.0	38.0	36.0	38.0
50-51	37.05885216801953	38.0	38.0	38.0	36.0	38.0
52-53	36.96349706517786	38.0	38.0	38.0	36.0	38.0
54-55	36.998397701507514	38.0	38.0	38.0	36.0	38.0
56-57	36.95682705014569	38.0	38.0	38.0	36.0	38.0
58-59	36.93234579747994	38.0	38.0	38.0	36.0	38.0
60-61	36.92205515541987	38.0	38.0	38.0	36.0	38.0
62-63	36.928363956889854	38.0	38.0	38.0	36.0	38.0
64-65	36.93606905432727	38.0	38.0	38.0	36.0	38.0
66-67	36.92810680020821	38.0	38.0	38.0	36.0	38.0
68-69	36.90620095858171	38.0	38.0	38.0	35.0	38.0
70-71	36.9036253158401	38.0	38.0	38.0	35.0	38.0
72-73	36.91669355843375	38.0	38.0	38.0	35.0	38.0
74-75	36.85139750049069	38.0	38.0	38.0	35.0	38.0
76-77	36.84896425339624	38.0	38.0	38.0	35.0	38.0
78-79	36.843396540261644	38.0	38.0	38.0	35.0	38.0
80-81	36.815889650186726	38.0	38.0	38.0	35.0	38.0
82-83	36.73766571387145	38.0	38.0	38.0	35.0	38.0
84-85	36.75474202689652	38.0	38.0	38.0	35.0	38.0
86-87	36.74754384610315	38.0	38.0	38.0	35.0	38.0
88-89	36.72091094377055	38.0	38.0	38.0	34.5	38.0
90-91	36.73440944356831	38.0	38.0	38.0	35.0	38.0
92-93	36.70476996554396	38.0	38.0	38.0	34.0	38.0
94-95	36.60628932489718	38.0	38.0	38.0	34.0	38.0
96-97	36.58359060336715	38.0	38.0	38.0	34.0	38.0
98-99	36.62443676260574	38.0	38.0	38.0	34.0	38.0
100	35.007291552212315	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	11.0
20	53.0
21	136.0
22	460.0
23	1191.0
24	2776.0
25	5688.0
26	9893.0
27	16068.0
28	24885.0
29	35488.0
30	47277.0
31	61868.0
32	81617.0
33	113786.0
34	177100.0
35	349883.0
36	1211098.0
37	3062539.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.94393617158298	13.101296642079763	11.18925468600323	21.765512500334015
2	14.585471497067742	19.078874256553497	42.07777410762387	24.25788013875489
3	19.38344283665278	24.912141344348452	30.426460272277726	25.277955546721042
4	12.753168553858352	17.889177655286485	38.98292540246964	30.37472838838553
5	13.705642697047823	37.61946056967358	34.97617468959428	13.698722043684317
6	29.99457305432078	37.5600006228588	18.771195702428052	13.674230620392358
7	26.777084409478913	32.85028454458544	22.401674336737077	17.970956709198568
8	24.274460809012844	36.78181160020693	21.159282489728113	17.784445101052114
9	25.567431097686754	17.351308320682314	20.972636313321814	36.10862426830912
10-11	24.239578793657067	26.772211116068778	28.691375577898587	20.29683451237557
12-13	25.244044345239868	24.154646997657167	28.73733063863597	21.863978018466995
14-15	22.43572971849666	25.654131505101773	26.186195180495446	25.723943595906125
16-17	22.126616813612003	28.957955300653364	26.806622142515092	22.108805743219538
18-19	22.146254167530945	27.84417226198287	28.57034104018574	21.439232530300448
20-21	22.77669470647593	27.01865383924052	28.538213235453846	21.666438218829704
22-23	22.484089210961937	27.233645619826515	28.315605228064815	21.966659941146734
24-25	22.470184393312415	27.212370792308494	28.477997177200194	21.8394476371789
26-27	22.332238532812195	27.41768662067364	28.503110096193872	21.746964750320295
28-29	22.261846046598592	27.191469569185006	28.629468788976748	21.917215595239654
30-31	22.33547592292069	26.91163637567961	28.756900098663614	21.995987602736086
32-33	22.00098528791697	27.417473937475407	28.574208125721732	22.00733264888589
34-35	22.370112408168534	27.26103363057194	28.528474972603245	21.84037898865628
36-37	22.21955153485514	27.282847958016276	28.73335802316929	21.764242483959293
38-39	22.00607711941343	27.228629638228735	28.716348394741015	22.048944847616823
40-41	22.38035560283534	27.019749523539037	28.750115156358596	21.849779717267033
42-43	22.42896900386851	27.012436578412753	28.61206666346326	21.94652775425548
44-45	22.180281301996928	27.33487745266901	28.510532840127144	21.974308405206916
46-47	22.501038768467673	27.031727720476923	28.18537763667111	22.28185587438429
48-49	22.32417810922865	27.293874066095565	28.132509252088184	22.249438572587604
50-51	22.134089821784073	27.466516271939646	28.163964045981775	22.235429860294502
52-53	22.213649507340392	27.396001278930754	28.244608612950422	22.14574060077843
54-55	22.327044792459038	27.151614489894556	28.27535541429197	22.245985303354438
56-57	22.397195604490435	27.15584292144953	28.052910403971925	22.39405107008811
58-59	21.91868455687353	27.25681072956119	28.44082932023176	22.383675393333522
60-61	22.346728756960214	27.79044239129299	28.17697372504682	21.685855126699966
62-63	21.65616103257694	28.269752863697974	28.12913319826114	21.944952905463943
64-65	21.51956087896984	28.491007511198696	27.86314220644906	22.126289403382405
66-67	21.71023296048364	28.922469742757954	27.426873281372178	21.940424015386228
68-69	21.817442403631365	28.73242125054281	27.371136890360777	22.07899945546505
70-71	21.816083245076968	28.061791540217122	27.47710071779937	22.64502449690654
72-73	22.190864322061707	27.35257313941759	27.514815821135475	22.94174671738523
74-75	22.112131633700763	27.39115868362798	27.771943545542317	22.724766137128942
76-77	22.45063559382806	27.235279931139495	27.19477959365507	23.11930488137737
78-79	22.610457374932054	27.3426993227587	26.792276608976728	23.254566693332514
80-81	22.480339911235216	27.62347056846275	26.93439179929218	22.961797721009855
82-83	22.453010648675324	27.324054743889302	27.05719743949379	23.16573716794158
84-85	22.563149167406156	27.695123395240113	26.97505304434054	22.76667439301319
86-87	22.883333075875452	27.575917989135583	27.08116079228475	22.459588142704217
88-89	22.666147197045305	27.78241885390871	27.29819064728994	22.253243301756047
90-91	22.73961439371889	27.36977454344537	27.198026982056362	22.69258408077938
92-93	22.666723763879933	27.036712756849973	27.369877368708934	22.92668611056116
94-95	22.01479527560848	27.525227010433113	27.8396934368842	22.6202842770742
96-97	22.1984226927047	27.320236115452772	28.081815651951285	22.399525539891236
98-99	23.285789753114976	29.241683821384846	28.381376248624402	19.091150176875775
100	22.44556198847709	30.30418797278451	22.049331586583836	25.200918452154557
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	629.0
1	646.5
2	886.5
3	1233.0
4	1679.5
5	2513.5
6	3218.0
7	3775.0
8	4696.0
9	5389.0
10	5357.0
11	5269.0
12	5782.5
13	7066.5
14	9544.0
15	12529.0
16	15494.5
17	18376.0
18	19496.5
19	18249.0
20	16065.5
21	14257.0
22	13695.0
23	15027.5
24	18569.0
25	24190.0
26	32427.5
27	42323.0
28	51780.5
29	62197.0
30	72949.5
31	83108.0
32	94481.0
33	106329.0
34	118998.0
35	130930.0
36	143911.0
37	157328.5
38	166930.5
39	174843.5
40	185077.5
41	194313.0
42	199487.0
43	206019.5
44	217762.0
45	235379.5
46	239652.0
47	234578.5
48	231616.5
49	223829.0
50	216767.5
51	208935.0
52	199189.5
53	189160.0
54	177661.5
55	164788.5
56	150471.0
57	134278.0
58	118415.0
59	103206.0
60	86528.5
61	69285.5
62	53990.5
63	41291.5
64	30732.5
65	22266.5
66	16082.0
67	12098.0
68	9130.5
69	6564.5
70	4699.5
71	3365.0
72	2384.5
73	1608.0
74	1018.5
75	663.0
76	435.0
77	267.0
78	163.5
79	108.0
80	66.5
81	43.5
82	33.0
83	19.5
84	11.5
85	10.0
86	9.0
87	6.5
88	3.5
89	2.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013591394244438629
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.0761924812087896E-4
26-27	3.0765331855059136E-4
28-29	0.0037412433026695643
30-31	0.006430821115735912
32-33	0.004437167917771176
34-35	0.001992275580068927
36-37	0.0
38-39	0.0
40-41	3.328631804078298E-4
42-43	0.0
44-45	0.0
46-47	4.595636443197184E-4
48-49	0.0014194672789637768
50-51	0.002950088157960899
52-53	0.0
54-55	0.002846608662270825
56-57	0.03875738730043506
58-59	0.060625715197164885
60-61	0.042161934591870835
62-63	0.013648428047715648
64-65	3.430946178019529E-4
66-67	0.0
68-69	0.0
70-71	0.0029709888834605477
72-73	0.00519731176372999
74-75	0.004288870294058952
76-77	0.002769082869824532
78-79	0.00805697752058758
80-81	0.015877612577494593
82-83	0.04107209847151744
84-85	0.01836384432168107
86-87	0.0018098577301963233
88-89	0.0012680233818858323
90-91	1.1745818606034439E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	27.0
20-21	135.0
22-23	351.0
24-25	432.0
26-27	1359.0
28-29	3448.0
30-31	9734.0
32-33	12542.0
34-35	17275.0
36-37	26038.0
38-39	15977.0
40-41	28949.0
42-43	32442.0
44-45	38363.0
46-47	38827.0
48-49	35753.0
50-51	18052.0
52-53	3244.0
54-55	2971.0
56-57	3016.0
58-59	19610.0
60-61	37532.0
62-63	36755.0
64-65	43327.0
66-67	48381.0
68-69	48725.0
70-71	60282.0
72-73	39049.0
74-75	37561.0
76-77	38726.0
78-79	39803.0
80-81	40627.0
82-83	37654.0
84-85	37870.0
86-87	38874.0
88-89	40766.0
90-91	41903.0
92-93	42134.0
94-95	50801.0
96-97	179083.0
98-99	403973.0
100-101	3549450.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.11182794192345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.39734553039102	80.5318718372234
2	6.372707172506193	11.230217558162542
3	1.3334046373887705	3.524661599596865
4	0.4420695813122914	1.5580623554778705
5	0.1953955430974356	0.8608329237009965
6	0.09135010630605883	0.4829414909589524
7	0.051623943457408804	0.3184076016521952
8	0.02922876426479946	0.20603198782840407
9	0.018666143985499616	0.148023726047056
>10	0.0660729198589266	0.8726466955167607
>50	0.0012948280477400537	0.07578399452280234
>100	7.489910894555765E-4	0.1251615198307344
>500	6.122552998566535E-5	0.0360095781606828
>1k	3.061276438987181E-5	0.029347131320767644
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.9224037120846718E-5	0.0	0.0	0.0
4	0.0	9.61201856042336E-5	0.0	0.0	0.0
5	0.0	9.61201856042336E-5	0.0	0.0	0.0
6	9.61201856042336E-5	9.61201856042336E-5	0.0	0.0	0.0
7	9.61201856042336E-5	9.61201856042336E-5	0.0	0.0	0.0
8	9.61201856042336E-5	1.1534422272508031E-4	0.0	0.0	0.0
9	9.61201856042336E-5	1.3456825984592703E-4	0.0	0.0	0.0
10-11	9.61201856042336E-5	1.3456825984592703E-4	0.0	0.0	0.0
12-13	9.61201856042336E-5	1.3456825984592703E-4	0.0	0.0	0.0
14-15	9.61201856042336E-5	2.0185238976889053E-4	0.0	0.0	3.8448074241693436E-5
16-17	1.5379229696677374E-4	2.2107642688973727E-4	0.0	0.0	3.8448074241693436E-5
18-19	1.5379229696677374E-4	2.3068844545016061E-4	0.0	9.612018560423359E-6	3.8448074241693436E-5
20-21	1.634043155271971E-4	2.3068844545016061E-4	0.0	2.8836055681270077E-5	3.8448074241693436E-5
22-23	1.7301633408762048E-4	2.8836055681270075E-4	0.0	4.806009280211679E-5	6.728412992296352E-5
24-25	1.7301633408762048E-4	3.268086310543942E-4	0.0	5.7672111362540154E-5	1.2495624128550366E-4
26-27	1.7301633408762048E-4	3.4603266817524096E-4	0.0	1.4418027840635037E-4	1.5379229696677374E-4
28-29	1.8262835264804385E-4	3.6525670529608765E-4	0.0	7.401254291525986E-4	1.5379229696677374E-4
30-31	1.922403712084672E-4	4.229288166586278E-4	0.0	0.0025279608813913437	1.5379229696677374E-4
32-33	2.0185238976889053E-4	4.229288166586278E-4	0.0	0.005747987099133168	1.5379229696677374E-4
34-35	2.114644083293139E-4	4.902129465815914E-4	0.0	0.009909991135796483	1.7301633408762048E-4
36-37	2.114644083293139E-4	5.574970765045549E-4	0.0	0.020531271645064297	2.0185238976889053E-4
38-39	2.114644083293139E-4	5.863331321858249E-4	0.0	0.16956561942442847	2.2107642688973727E-4
40-41	2.114644083293139E-4	7.49737447713022E-4	0.0	0.3239442495233881	2.3068844545016061E-4
42-43	2.114644083293139E-4	7.689614848338688E-4	0.0	0.3469073618642394	2.3068844545016061E-4
44-45	2.2107642688973727E-4	8.266335961964089E-4	0.0	0.3763874227890579	2.4030046401058396E-4
46-47	2.3068844545016061E-4	8.650816704381024E-4	0.0	0.40944315461835384	2.499124825710073E-4
48-49	2.3068844545016061E-4	8.650816704381024E-4	0.0	0.44157613266584916	2.6913651969185406E-4
50-51	2.499124825710073E-4	9.515898374819126E-4	0.0	0.47401669530727797	2.6913651969185406E-4
52-53	2.499124825710073E-4	9.612018560423359E-4	0.0	0.9078359289948654	2.7874853825227743E-4
54-55	2.499124825710073E-4	9.612018560423359E-4	0.0	1.4887671067497323	2.883605568127008E-4
56-57	2.499124825710073E-4	9.612018560423359E-4	0.0	2.118460054661627	2.883605568127008E-4
58-59	2.499124825710073E-4	9.612018560423359E-4	0.0	2.767204023360281	2.883605568127008E-4
60-61	2.499124825710073E-4	9.612018560423359E-4	0.0	2.9397878166126823	2.883605568127008E-4
62-63	3.075845939335475E-4	9.996499302840293E-4	0.0	2.9457376561015844	3.075845939335475E-4
64-65	3.075845939335475E-4	0.0011534422272508032	0.0	2.9512837908109484	3.1719661249397086E-4
66-67	3.268086310543942E-4	0.00117266626437165	0.0	2.956233980369567	3.268086310543942E-4
68-69	3.4603266817524096E-4	0.00117266626437165	0.0	2.961136109835383	3.268086310543942E-4
70-71	3.5564468673566433E-4	0.0011822782829320733	0.0	2.9652212177235624	3.268086310543942E-4
72-73	3.74868723856511E-4	0.0011918903014924965	0.0	2.9684220199041835	3.6525670529608765E-4
74-75	3.844807424169344E-4	0.0011918903014924965	0.0	2.971372909602233	3.6525670529608765E-4
76-77	3.844807424169344E-4	0.0012399503942946133	0.0	2.9743045752631625	3.6525670529608765E-4
78-79	3.844807424169344E-4	0.0012495624128550367	0.0	2.975996290529797	3.6525670529608765E-4
80-81	3.844807424169344E-4	0.00125917443141546	0.0	2.976640295773345	3.74868723856511E-4
82-83	3.844807424169344E-4	0.0012687864499758834	0.0	2.9770632245900037	4.229288166586278E-4
84-85	3.940927609773577E-4	0.0012880104870967302	0.0	2.9773035250540145	4.229288166586278E-4
86-87	4.1331679809820444E-4	0.0012880104870967302	0.0	2.977351585146817	4.229288166586278E-4
88	4.4215285377947455E-4	0.001307234524217577	0.0	2.977361197165377	4.229288166586278E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6560	0.0	48.386665	1
GTATCAA	11905	0.0	38.54901	1
TCAACGC	13230	0.0	33.827393	4
ATCAACG	13205	0.0	33.72659	3
TATCAAC	13445	0.0	33.291573	2
CAACGCA	13590	0.0	32.899265	5
AACGCAG	14095	0.0	31.813198	6
ACGCAGA	16260	0.0	27.523762	7
CGCAGAG	16790	0.0	26.654936	8
GTGGTAT	3380	0.0	26.408222	1
GCGCAGA	1285	0.0	25.752089	1
TGGTATC	3260	0.0	24.308369	2
GTCAACG	670	0.0	24.045229	1
GCAGAGT	20070	0.0	22.27708	9
GAGTACT	11170	0.0	21.046303	12-13
GTACATG	15855	0.0	21.008602	1
TACATGG	15385	0.0	20.829666	2
ACATGGG	15620	0.0	19.983583	3
CAGAGTA	19280	0.0	19.723824	10-11
CATGGGG	10900	0.0	18.612068	4
>>END_MODULE