FastQCFastQC Report
Fri 27 May 2016
SRR2140141_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140141_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5879279
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64650.047.923991
GTATCAA114150.036.878851
TCAACGC134850.030.4486264
ATCAACG135300.030.3156433
CAACGCA140500.029.2547175
TATCAAC144950.028.6280982
AACGCAG146150.028.2411866
CTTATAC65700.025.2119371
ACGCAGA172750.023.867787
CGCAGAG175100.023.5229518
TATACAC88450.020.8582253
GCAGAGT207650.019.7529839
TACATGG144900.019.6349332
TGGTATC32500.019.4096762
GTACATG149900.019.4094091
ACATGGG145450.018.7312493
GAGTACT126950.018.60507212-13
GTGGTAT36700.018.4746111
TTATACA96100.018.040242
CAGAGTA202750.017.76505510-11
Produced by FastQC (version 0.11.5)