##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140141_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5879279
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.657206436367453	32.0	28.0	33.0	18.0	34.0
2	31.159952776522427	33.0	31.0	33.0	27.0	34.0
3	32.36261963414221	33.0	33.0	33.0	30.0	34.0
4	32.44813573228962	33.0	33.0	34.0	31.0	34.0
5	32.92187596472289	33.0	33.0	34.0	32.0	34.0
6	36.731287458887394	38.0	37.0	38.0	34.0	38.0
7	36.92972794793375	38.0	38.0	38.0	35.0	38.0
8	37.1378434328427	38.0	38.0	38.0	36.0	38.0
9	37.174037666863576	38.0	38.0	38.0	36.0	38.0
10-11	37.26191153711195	38.0	38.0	38.0	36.0	38.0
12-13	37.309148451706406	38.0	38.0	38.0	36.5	38.0
14-15	37.3102689972699	38.0	38.0	38.0	36.5	38.0
16-17	37.35823916504047	38.0	38.0	38.0	37.0	38.0
18-19	37.35728658224929	38.0	38.0	38.0	37.0	38.0
20-21	37.37620388405685	38.0	38.0	38.0	37.0	38.0
22-23	37.31290407259786	38.0	38.0	38.0	37.0	38.0
24-25	37.383564266411724	38.0	38.0	38.0	37.0	38.0
26-27	37.34919515484296	38.0	38.0	38.0	37.0	38.0
28-29	37.31617442423115	38.0	38.0	38.0	37.0	38.0
30-31	37.3318380579265	38.0	38.0	38.0	37.0	38.0
32-33	37.31710592087815	38.0	38.0	38.0	37.0	38.0
34-35	37.22823503958516	38.0	38.0	38.0	37.0	38.0
36-37	37.24968237979368	38.0	38.0	38.0	37.0	38.0
38-39	37.243490841796145	38.0	38.0	38.0	37.0	38.0
40-41	37.27021664725807	38.0	38.0	38.0	37.0	38.0
42-43	37.2501276512655	38.0	38.0	38.0	37.0	38.0
44-45	37.22250004290859	38.0	38.0	38.0	37.0	38.0
46-47	37.1965362956209	38.0	38.0	38.0	37.0	38.0
48-49	37.14494924851585	38.0	38.0	38.0	36.5	38.0
50-51	37.109461704819324	38.0	38.0	38.0	36.0	38.0
52-53	37.01827752221458	38.0	38.0	38.0	36.0	38.0
54-55	37.061635137457635	38.0	38.0	38.0	36.0	38.0
56-57	37.031215837091196	38.0	38.0	38.0	36.0	38.0
58-59	37.014674389142584	38.0	38.0	38.0	36.0	38.0
60-61	37.01356967020614	38.0	38.0	38.0	36.0	38.0
62-63	37.0213237298788	38.0	38.0	38.0	36.0	38.0
64-65	37.03388225890528	38.0	38.0	38.0	36.0	38.0
66-67	37.032720410622034	38.0	38.0	38.0	36.0	38.0
68-69	37.01909677110411	38.0	38.0	38.0	36.0	38.0
70-71	37.02028332121874	38.0	38.0	38.0	36.0	38.0
72-73	37.033529869090955	38.0	38.0	38.0	36.0	38.0
74-75	36.97756581489965	38.0	38.0	38.0	36.0	38.0
76-77	36.98297642033149	38.0	38.0	38.0	36.0	38.0
78-79	36.98902334020111	38.0	38.0	38.0	36.0	38.0
80-81	36.97696254593474	38.0	38.0	38.0	36.0	38.0
82-83	36.932642226674645	38.0	38.0	38.0	36.0	38.0
84-85	36.92947358784134	38.0	38.0	38.0	36.0	38.0
86-87	36.92654627426246	38.0	38.0	38.0	36.0	38.0
88-89	36.91196744231682	38.0	38.0	38.0	35.0	38.0
90-91	36.91944684853988	38.0	38.0	38.0	35.5	38.0
92-93	36.89981007988386	38.0	38.0	38.0	35.0	38.0
94-95	36.83611145851175	38.0	38.0	38.0	35.0	38.0
96-97	36.86568262260693	38.0	38.0	38.0	35.0	38.0
98-99	36.89452940430739	38.0	38.0	38.0	35.0	38.0
100	35.44016886694208	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	2.0
19	9.0
20	16.0
21	93.0
22	350.0
23	1171.0
24	2962.0
25	5868.0
26	10577.0
27	17329.0
28	26923.0
29	38188.0
30	51299.0
31	65950.0
32	85723.0
33	113418.0
34	164719.0
35	286390.0
36	784235.0
37	4224054.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.38260473775781	21.128390062795116	14.230996691941309	24.258008507505767
2	14.753078480847275	20.273402306547013	42.71376864848975	22.259750564115965
3	18.512099868028034	25.93462565732975	30.07181662921593	25.481457845426288
4	12.911106957162605	17.872021382213703	38.43214448574391	30.784727174879777
5	13.701935220288068	36.95243923617165	35.74067500453712	13.604950539003166
6	30.14563520458886	37.88381194360737	18.388411232057535	13.58214161974623
7	26.40927569520004	32.54439872644247	22.91517378236345	18.131151795994032
8	23.719439067273385	37.910720004952985	21.027017088319845	17.342823839453782
9	24.95758408471515	17.51553209160511	21.352465157717468	36.17441866596227
10-11	24.201326387130123	27.07688306678421	28.709600275816133	20.012190270269535
12-13	24.870132885341892	24.30075184389106	29.023856496689476	21.805258774077572
14-15	22.01524200501456	25.91738714900245	26.395881195636406	25.67148965034658
16-17	21.805794554060114	29.129175533258415	27.19028132531217	21.874748587369304
18-19	21.81430750267167	28.035699615548097	28.873004666048335	21.276988215731897
20-21	22.410367976628187	27.289596397888598	28.80575879260143	21.494276832881784
22-23	22.152775102318767	27.357243814841965	28.70792041610099	21.782060666738275
24-25	22.06433591699443	27.447583137741464	28.791638613593594	21.696442331670504
26-27	22.004209232701772	27.674271768974144	28.72961655290159	21.59190244542249
28-29	21.79949867393888	27.54188657431928	28.818335795209983	21.84027895653186
30-31	22.308356692759908	27.4137357430435	28.75716780800659	21.520739756190004
32-33	22.11975838975413	27.53362960915563	28.772246812347696	21.574365188742544
34-35	22.092172997384413	27.60623523670317	28.834707428580124	21.466884337332296
36-37	22.116643959792743	27.403430704699787	28.767688297199008	21.712237038308462
38-39	21.82788446723339	27.422873798287828	28.967882393315293	21.781359341163494
40-41	21.995176592635858	27.302321550021745	28.936383756307656	21.76611810103474
42-43	22.079501160701202	27.34893541234234	28.828077913186373	21.743485513770082
44-45	21.934455896116656	27.561692881069256	28.708023709591508	21.795827513222584
46-47	22.219772216796787	27.386647694769234	28.32396958970615	22.069610498727826
48-49	22.062084989448348	27.513162343363028	28.416559472156738	22.008193195031886
50-51	21.926020916229433	27.72211883484782	28.448695750064086	21.90316449885866
52-53	22.098265152067757	27.772345712709967	28.382602324388888	21.74678681083339
54-55	22.023585311719003	27.68642858476743	28.280119643692448	22.00986645982112
56-57	22.163468642475674	27.88368718560104	28.067134186690456	21.88570998523283
58-59	21.921333328646366	28.09470719931087	28.289617190117628	21.694342281925135
60-61	22.062905386408342	28.26434119623465	28.041756635969474	21.63099678138753
62-63	21.83122240059325	28.44618653899162	28.042980899508922	21.67961016090621
64-65	21.80618531373284	28.415447227986647	28.050776723341585	21.727590734938932
66-67	21.855705255326853	28.47318798261976	27.88213305208179	21.788973709971597
68-69	21.92362096086516	28.302356588493904	27.978116163614743	21.795906287026188
70-71	21.97676959241155	27.85479597907141	28.156007979943826	22.012426448573216
72-73	22.093406718257413	27.857660119522187	28.05639613632524	21.992537025895157
74-75	21.923778532666386	27.997451204398615	28.040728501838043	22.038041761096956
76-77	22.176039823066702	27.777124063504015	27.94942303178198	22.097413081647304
78-79	22.193210189183954	27.812851706733394	27.91244144736548	22.08149665671717
80-81	22.174862346687075	27.812312539761187	27.862392531343072	22.15043258220867
82-83	22.198411746289658	27.780619840387278	27.955453310367535	22.06551510295553
84-85	22.308631674915887	27.782088840655174	27.89309261083126	22.016186873597686
86-87	22.17336500457381	27.752497867172437	27.99943518589265	22.0747019423611
88-89	22.219539510165916	27.80155620040136	27.89538361041307	22.08352067901965
90-91	22.302579578506496	27.73152199451811	27.955511855938926	22.01038657103647
92-93	22.36590129575764	27.78918259763262	27.846316189952695	21.99859991665704
94-95	22.21135050900096	27.902888724424713	27.887694994458695	21.99806577211563
96-97	22.315708269106953	27.970104943378082	27.97524939108192	21.73893739643304
98-99	23.314182761296358	29.870676868279944	28.224755983607587	18.59038438681611
100	22.215435366458344	30.728098475124376	22.28360417731539	24.772861981101894
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	953.0
1	863.5
2	1047.0
3	1495.5
4	2006.5
5	2795.5
6	3527.5
7	4472.5
8	5575.5
9	6087.5
10	6004.0
11	6019.0
12	6804.0
13	8601.0
14	11901.0
15	15617.0
16	18354.0
17	19903.5
18	20095.0
19	19125.5
20	17759.0
21	17033.5
22	17959.5
23	20828.5
24	26077.5
25	34271.0
26	44918.5
27	57139.0
28	69095.0
29	81597.5
30	94515.5
31	107989.5
32	122268.5
33	136342.5
34	151501.5
35	165604.0
36	179987.0
37	193375.0
38	202725.5
39	210755.5
40	221403.5
41	231376.5
42	237919.0
43	244317.5
44	252836.0
45	264756.0
46	267269.5
47	263422.0
48	259574.0
49	249914.5
50	239838.5
51	229871.5
52	217933.0
53	204259.5
54	189988.0
55	174366.5
56	157806.5
57	141350.5
58	124347.0
59	107874.5
60	90344.5
61	72438.5
62	56824.5
63	43633.0
64	32569.5
65	23649.0
66	17194.5
67	13049.5
68	9951.5
69	7196.0
70	5170.0
71	3753.0
72	2621.0
73	1718.0
74	1112.5
75	742.0
76	496.5
77	309.0
78	198.0
79	138.5
80	97.5
81	68.5
82	41.0
83	21.5
84	13.5
85	10.0
86	6.5
87	5.0
88	4.0
89	2.5
90	1.5
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.017944377193189844
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.273212810368974E-4
26-27	3.4874093464952135E-4
28-29	0.003735004255692776
30-31	0.005866654768738854
32-33	0.004017860798781437
34-35	0.0021463319528969922
36-37	0.0
38-39	0.0
40-41	3.7307217124533227E-4
42-43	0.0
44-45	0.0
46-47	3.3650563691218886E-4
48-49	0.0013655733600735802
50-51	0.0024366397245644033
52-53	0.0
54-55	0.003088012735365325
56-57	0.04108155872864777
58-59	0.06522960617995051
60-61	0.044172872058808176
62-63	0.013892959540656271
64-65	4.2652447667579336E-4
66-67	0.0
68-69	9.665833761771416E-6
70-71	0.003038899773665481
72-73	0.005295131462505431
74-75	0.004116257052578556
76-77	0.002976825613733947
78-79	0.007757000388256144
80-81	0.01608905687773855
82-83	0.04458978639152028
84-85	0.020205983960859975
86-87	0.0017412687770773892
88-89	0.0011619028472269375
90-91	7.541015042170433E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	33.0
20-21	134.0
22-23	274.0
24-25	382.0
26-27	1072.0
28-29	2770.0
30-31	10331.0
32-33	13085.0
34-35	18368.0
36-37	29055.0
38-39	32655.0
40-41	32649.0
42-43	36744.0
44-45	44066.0
46-47	44864.0
48-49	42097.0
50-51	37316.0
52-53	35073.0
54-55	38729.0
56-57	40277.0
58-59	43170.0
60-61	45992.0
62-63	43714.0
64-65	47848.0
66-67	52403.0
68-69	53211.0
70-71	60127.0
72-73	45240.0
74-75	44297.0
76-77	46762.0
78-79	48283.0
80-81	48249.0
82-83	45636.0
84-85	45042.0
86-87	46527.0
88-89	48968.0
90-91	49701.0
92-93	49546.0
94-95	57808.0
96-97	180821.0
98-99	393345.0
100-101	3922615.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.33366403019207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.8425846035397	79.33615763399835
2	6.645987761567198	11.608369246349557
3	1.5157980291289008	3.9714058744071217
4	0.4771078703540952	1.666703138226599
5	0.21253457733475864	0.9280711685876343
6	0.10837514431880763	0.5678879065897394
7	0.06326679050461617	0.3867724438339087
8	0.03652846701407629	0.25521318925962333
9	0.024089885774548868	0.18934721916841388
>10	0.07237225094449692	0.9207113767360464
>50	8.367133790232017E-4	0.04848452696552043
>100	4.6194068081784924E-4	0.08243476245896268
>500	5.5965458928705826E-5	0.038441513418515524
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.7008888334777104E-5	0.0
4	0.0	5.1026665004331315E-5	0.0	3.401777666955421E-5	0.0
5	0.0	5.1026665004331315E-5	0.0	5.1026665004331315E-5	0.0
6	5.1026665004331315E-5	5.1026665004331315E-5	0.0	5.1026665004331315E-5	0.0
7	5.1026665004331315E-5	5.1026665004331315E-5	0.0	5.1026665004331315E-5	0.0
8	5.1026665004331315E-5	8.504444167388553E-5	0.0	6.803555333910841E-5	0.0
9	5.1026665004331315E-5	1.5307999501299393E-4	0.0	6.803555333910841E-5	0.0
10-11	5.9531109171719865E-5	1.5307999501299393E-4	0.0	6.803555333910841E-5	0.0
12-13	6.803555333910841E-5	1.6158443918038248E-4	0.0	8.504444167388553E-5	0.0
14-15	6.803555333910841E-5	2.7214221335643366E-4	0.0	1.1055777417605118E-4	2.5513332502165657E-5
16-17	1.7008888334777106E-4	2.8915110169121076E-4	0.0	1.1906221834343973E-4	3.401777666955421E-5
18-19	1.785933275151596E-4	3.1466443419337644E-4	0.0	1.3607110667821683E-4	6.803555333910841E-5
20-21	1.8709777168254816E-4	3.401777666955421E-4	0.0	1.4457555084560538E-4	6.803555333910841E-5
22-23	1.956022158499367E-4	3.401777666955421E-4	0.0	1.5307999501299393E-4	6.803555333910841E-5
24-25	2.3812443668687946E-4	3.571866550303192E-4	0.0	1.7008888334777106E-4	6.803555333910841E-5
26-27	2.3812443668687946E-4	3.741955433650963E-4	0.0	2.126111041847138E-4	6.803555333910841E-5
28-29	2.3812443668687946E-4	3.8269998753248484E-4	0.0	4.5923998503898187E-4	6.803555333910841E-5
30-31	2.7214221335643366E-4	4.082133200346505E-4	0.0	0.0013947288434517226	6.803555333910841E-5
32-33	2.7214221335643366E-4	4.082133200346505E-4	0.0	0.0038014865428226824	8.504444167388553E-5
34-35	2.7214221335643366E-4	4.8475331754114744E-4	0.0	0.00722877754228027	8.504444167388553E-5
36-37	2.7214221335643366E-4	5.442844267128673E-4	0.0	0.013096844017778369	9.354888584127408E-5
38-39	2.7214221335643366E-4	5.69797759215033E-4	0.0	0.029476403484168724	1.1055777417605118E-4
40-41	2.7214221335643366E-4	5.953110917171987E-4	0.0	0.04720816957317385	1.1906221834343973E-4
42-43	2.7214221335643366E-4	6.038155358845872E-4	0.0	0.05692024481233158	1.1906221834343973E-4
44-45	2.7214221335643366E-4	6.803555333910842E-4	0.0	0.06821414666662358	1.2756666251082828E-4
46-47	2.7214221335643366E-4	7.483910867301926E-4	0.0	0.08004382850346106	1.6158443918038248E-4
48-49	2.7214221335643366E-4	7.568955308975812E-4	0.0	0.09205210366781369	1.8709777168254816E-4
50-51	2.8064665752382224E-4	8.249310842366896E-4	0.0	0.10424747660384888	2.0410666001732526E-4
52-53	2.8915110169121076E-4	8.334355284040781E-4	0.0	0.14205823537205836	2.126111041847138E-4
54-55	2.976555458585993E-4	8.419399725714666E-4	0.0	0.19030394713365362	2.2111554835210236E-4
56-57	3.0615999002598786E-4	8.504444167388552E-4	0.0	0.25500575835914574	2.2111554835210236E-4
58-59	3.1466443419337644E-4	8.589488609062438E-4	0.0	0.2949766459458719	2.2111554835210236E-4
60-61	3.3167332252815354E-4	8.844621934084094E-4	0.0	0.30987643212713667	2.3812443668687946E-4
62-63	3.571866550303192E-4	9.014710817431866E-4	0.0	0.3224970272715413	2.3812443668687946E-4
64-65	3.571866550303192E-4	0.0010035244117518492	0.0	0.3293771226029586	2.3812443668687946E-4
66-67	3.912044316998734E-4	0.0010205333000866262	0.0	0.33543228685013926	2.3812443668687946E-4
68-69	3.912044316998734E-4	0.0010460466325887918	0.0	0.3410792377772853	2.3812443668687946E-4
70-71	4.1671776420203904E-4	0.0010800644092583461	0.0	0.3455780887418338	2.5513332502165656E-4
72-73	4.337266525368162E-4	0.0010885688534257346	0.0	0.34925200862214567	3.0615999002598786E-4
74-75	4.422310967042047E-4	0.001156604406764843	0.0	0.35248369740575336	3.0615999002598786E-4
76-77	4.592399850389818E-4	0.0012671621809408943	0.0	0.35541773064350235	3.1466443419337644E-4
78-79	4.592399850389818E-4	0.0012926755134430598	0.0	0.3575268327970147	3.2316887836076496E-4
80-81	4.592399850389818E-4	0.0012926755134430598	0.0	0.35807111722372764	3.3167332252815354E-4
82-83	4.592399850389818E-4	0.0012926755134430598	0.0	0.3584708260995949	3.401777666955421E-4
84-85	4.592399850389818E-4	0.0012926755134430598	0.0	0.35873446386878394	3.401777666955421E-4
86-87	4.592399850389818E-4	0.001309684401777837	0.0	0.35891305719629907	3.401777666955421E-4
88	4.592399850389818E-4	0.0013437021784473912	0.0	0.35892156164046646	3.401777666955421E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6465	0.0	47.92399	1
GTATCAA	11415	0.0	36.87885	1
TCAACGC	13485	0.0	30.448626	4
ATCAACG	13530	0.0	30.315643	3
CAACGCA	14050	0.0	29.254717	5
TATCAAC	14495	0.0	28.628098	2
AACGCAG	14615	0.0	28.241186	6
CTTATAC	6570	0.0	25.211937	1
ACGCAGA	17275	0.0	23.86778	7
CGCAGAG	17510	0.0	23.522951	8
TATACAC	8845	0.0	20.858225	3
GCAGAGT	20765	0.0	19.752983	9
TACATGG	14490	0.0	19.634933	2
TGGTATC	3250	0.0	19.409676	2
GTACATG	14990	0.0	19.409409	1
ACATGGG	14545	0.0	18.731249	3
GAGTACT	12695	0.0	18.605072	12-13
GTGGTAT	3670	0.0	18.474611	1
TTATACA	9610	0.0	18.04024	2
CAGAGTA	20275	0.0	17.765055	10-11
>>END_MODULE