FastQCFastQC Report
Fri 27 May 2016
SRR2140140_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140140_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5030141
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC125800.057.618311
GGTATCA60200.050.434331
TATACAC157650.046.9726833
TTATACA156600.046.8718722
GTATCAA104850.034.1677321
TACACAT225150.033.149545
ATACACA247950.030.858044
ACACATC242800.030.0356856
TCAACGC122000.028.8455644
ATCAACG122950.028.622683
CACATCT259350.028.0707767
CAACGCA125550.027.9635165
TATCAAC128950.027.5232432
AACGCAG130800.026.9048826
CATCTCC280450.026.0777789
ACGCAGA149350.023.5910857
CGCAGAG151250.023.2671648
GTGGTAT27900.020.6284371
TGGTATC26150.019.616652
GAGTACT100200.019.59970912-13
Produced by FastQC (version 0.11.5)