##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140140_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5030141
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.913314954789538	33.0	30.0	33.0	18.0	34.0
2	31.294078833973042	33.0	31.0	33.0	27.0	34.0
3	32.42248537367044	33.0	33.0	34.0	31.0	34.0
4	32.508104245984356	33.0	33.0	34.0	31.0	34.0
5	32.952275890477026	33.0	33.0	34.0	32.0	34.0
6	36.75574342747052	38.0	37.0	38.0	34.0	38.0
7	36.98781326408147	38.0	38.0	38.0	35.0	38.0
8	37.170218687706765	38.0	38.0	38.0	36.0	38.0
9	37.225392687799406	38.0	38.0	38.0	36.0	38.0
10-11	37.29175265663527	38.0	38.0	38.0	36.5	38.0
12-13	37.340164619639886	38.0	38.0	38.0	37.0	38.0
14-15	37.33636353334827	38.0	38.0	38.0	37.0	38.0
16-17	37.37305057651466	38.0	38.0	38.0	37.0	38.0
18-19	37.376521453374764	38.0	38.0	38.0	37.0	38.0
20-21	37.39886420656015	38.0	38.0	38.0	37.0	38.0
22-23	37.32337602891093	38.0	38.0	38.0	37.0	38.0
24-25	37.39531218836372	38.0	38.0	38.0	37.0	38.0
26-27	37.363033882669114	38.0	38.0	38.0	37.0	38.0
28-29	37.32722986700035	38.0	38.0	38.0	37.0	38.0
30-31	37.34746759819171	38.0	38.0	38.0	37.0	38.0
32-33	37.325971629278385	38.0	38.0	38.0	37.0	38.0
34-35	37.23395739901498	38.0	38.0	38.0	37.0	38.0
36-37	37.25060260116092	38.0	38.0	38.0	37.0	38.0
38-39	37.19317930419264	38.0	38.0	38.0	37.0	38.0
40-41	37.28383803730275	38.0	38.0	38.0	37.0	38.0
42-43	37.27733373149502	38.0	38.0	38.0	37.0	38.0
44-45	37.240353754186714	38.0	38.0	38.0	37.0	38.0
46-47	37.211713280913656	38.0	38.0	38.0	37.0	38.0
48-49	37.15841198822217	38.0	38.0	38.0	36.5	38.0
50-51	37.11465460520853	38.0	38.0	38.0	36.0	38.0
52-53	37.01079918771383	38.0	38.0	38.0	36.0	38.0
54-55	37.047889194390486	38.0	38.0	38.0	36.0	38.0
56-57	37.01120637158303	38.0	38.0	38.0	36.0	38.0
58-59	36.974595819205454	38.0	38.0	38.0	36.0	38.0
60-61	36.945210726327765	38.0	38.0	38.0	36.0	38.0
62-63	36.955735131684975	38.0	38.0	38.0	36.0	38.0
64-65	36.96192202320546	38.0	38.0	38.0	36.0	38.0
66-67	36.946086270130856	38.0	38.0	38.0	36.0	38.0
68-69	36.92144300867826	38.0	38.0	38.0	36.0	38.0
70-71	36.922737817307514	38.0	38.0	38.0	36.0	38.0
72-73	36.93142792259655	38.0	38.0	38.0	36.0	38.0
74-75	36.88909640638518	38.0	38.0	38.0	36.0	38.0
76-77	36.9102148276169	38.0	38.0	38.0	36.0	38.0
78-79	36.926725991299875	38.0	38.0	38.0	36.0	38.0
80-81	36.92093469338403	38.0	38.0	38.0	36.0	38.0
82-83	36.882881096640475	38.0	38.0	38.0	36.0	38.0
84-85	36.88569438350629	38.0	38.0	38.0	35.5	38.0
86-87	36.89071298452273	38.0	38.0	38.0	35.0	38.0
88-89	36.88207140169334	38.0	38.0	38.0	35.0	38.0
90-91	36.89930638780149	38.0	38.0	38.0	35.0	38.0
92-93	36.883244625806725	38.0	38.0	38.0	35.0	38.0
94-95	36.817566148852265	38.0	38.0	38.0	35.0	38.0
96-97	36.85823966188294	38.0	38.0	38.0	35.0	38.0
98-99	36.890500250997405	38.0	38.0	38.0	35.0	38.0
100	35.492192650823064	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	5.0
19	16.0
20	44.0
21	132.0
22	372.0
23	1126.0
24	2778.0
25	5496.0
26	9885.0
27	15928.0
28	24072.0
29	33749.0
30	45312.0
31	57027.0
32	71863.0
33	94935.0
34	138492.0
35	242156.0
36	681852.0
37	3604897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.645616494646966	22.116576851424245	14.453471582605737	24.78433507132305
2	14.080624613379692	20.478254912998377	43.54537209899309	21.89574837462884
3	17.348479893505967	27.220350284415485	31.64756216575241	23.783607656326136
4	12.252340441351445	19.495596644308776	37.81220049298817	30.43986242135161
5	13.061721331469634	37.76874246666247	36.21413395767634	12.955402244191564
6	28.6912633264157	38.74799931055611	18.570195149599186	13.990542213429007
7	25.32718267738419	33.453376356646864	22.173116021996204	19.046324943972742
8	23.672656492134116	37.786893051308105	21.617187271688806	16.923263184868972
9	24.422178225222712	17.70652154681151	20.61246394484767	37.25883628311811
10-11	23.532541135526817	27.23314316636452	29.001214876481598	20.23310082162707
12-13	24.76087648437688	24.259240446738968	28.700438019530665	22.279445049353487
14-15	22.0593617554657	26.22442392767916	25.54243509277374	26.173779224081393
16-17	22.071130809255646	30.008144900908345	26.158898925497315	21.761825364338694
18-19	22.047185158427965	28.099361429431102	27.882637882317812	21.97081552982312
20-21	22.317270459550574	27.098836946349238	28.007003868230658	22.57688872586953
22-23	21.81651631501777	28.02343994632073	28.059315589353613	22.100728149307887
24-25	21.90254432492091	28.20688986017914	28.513371191251945	21.377194623648005
26-27	21.68410272726369	28.08100261491196	28.794684669457233	21.44020998836712
28-29	20.681032415389172	27.080140236486695	28.99182570522825	23.247001642895885
30-31	21.098988405415792	26.90861909337599	29.544056960240976	22.448335540967236
32-33	21.058255588736255	27.53622219058775	29.473689359563675	21.931832861112316
34-35	21.181242782242123	28.482419214187203	29.728189703206198	20.60814830036448
36-37	21.580621170148735	27.79881126668013	28.46976363455469	22.150803928616448
38-39	21.75790826323512	27.133734936098985	29.128301248654488	21.980055552011407
40-41	21.74940705472556	27.080269854985527	29.507437043575933	21.66288604671298
42-43	22.41514745407441	26.815012945523627	29.577460738211194	21.192378862190772
44-45	21.47422221301628	27.625636936078546	29.37666224781474	21.523478603090435
46-47	22.667565139451103	26.705716641113998	28.290508476915093	22.336209742519806
48-49	22.213809024754173	26.798698075859072	28.70327110813871	22.284221791248036
50-51	21.540628263048845	27.350038866655556	29.246780600336088	21.86255226995951
52-53	21.750575275746893	27.763990745836793	29.73661747403633	20.748816504379988
54-55	21.58279203589354	27.9997004837641	28.673914556186524	21.743592924155834
56-57	22.413267290299853	28.86565160599497	27.46060220015556	21.26047890354962
58-59	21.097931946754255	30.013180631193926	28.715799914476065	20.173087507575755
60-61	21.987276562636428	31.10982209544636	26.786166946313855	20.116734395603356
62-63	21.06613657910579	31.91728444127221	26.784269142451343	20.232309837170657
64-65	21.156133950265385	31.657654722904116	26.86424962707822	20.321961699752283
66-67	21.41699762267771	31.451359893229036	26.73564222458049	20.396000259512764
68-69	21.599299489325073	30.566034439571393	27.201637861572653	20.63302820953088
70-71	21.850557475024836	29.46415410702214	27.59021991063671	21.095068507316313
72-73	22.24324697579578	28.778553586124367	27.775585448370478	21.202613989709373
74-75	21.954865826251986	28.757189157274137	27.886356958063775	21.401588058410102
76-77	22.04591508373929	28.40805535553533	27.964953939995947	21.581075620729443
78-79	21.992440332020802	28.459558295437237	27.95668731883445	21.59131405370751
80-81	22.10518836192615	28.368828379010523	27.943868714972332	21.582114544090995
82-83	22.063254591022265	28.242127624825102	28.0600899990271	21.63452778512553
84-85	22.166529405631636	28.199915431756168	28.043798556968767	21.58975660564343
86-87	21.998015577741352	28.233637900708068	28.084872786925917	21.683473734624652
88-89	22.07608755815665	28.212643623208333	28.049528248843604	21.661740569791416
90-91	22.129613335025446	28.112010527823855	28.159243423074926	21.599132714075772
92-93	22.163639210881595	28.165045147964875	28.035199476809282	21.636116164344248
94-95	21.9852047926704	28.319170681606604	28.072602519270035	21.62302200645296
96-97	22.083399762928053	28.33787043912875	28.193899390211847	21.384830407731354
98-99	23.08917842995497	30.292696575035766	28.446984890206924	18.171140104802344
100	29.112593846054985	41.268830793286995	29.618575360658024	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1215.0
1	1034.0
2	897.0
3	1029.0
4	1369.0
5	1993.5
6	2485.0
7	3015.0
8	3693.0
9	4144.5
10	4359.0
11	4651.0
12	5413.0
13	6876.0
14	9656.5
15	12961.0
16	15582.5
17	17175.0
18	17523.5
19	16969.0
20	16232.5
21	15732.0
22	16456.5
23	19338.0
24	24421.5
25	31762.5
26	41728.5
27	53115.0
28	63915.0
29	75584.0
30	87441.0
31	98857.5
32	112140.5
33	126496.0
34	140310.0
35	152268.5
36	165302.5
37	177750.0
38	186147.5
39	193365.5
40	202908.0
41	211394.5
42	219477.0
43	244679.0
44	282951.0
45	298177.0
46	282103.0
47	256614.5
48	227015.0
49	204840.5
50	195198.5
51	185259.5
52	173105.0
53	160236.5
54	147359.0
55	133770.5
56	119473.5
57	105378.5
58	91839.0
59	78187.0
60	64863.0
61	52049.5
62	40258.5
63	30554.0
64	22967.5
65	17252.5
66	12749.5
67	9677.0
68	7275.0
69	5291.0
70	3860.0
71	2736.0
72	1902.5
73	1282.5
74	852.0
75	575.5
76	380.0
77	243.0
78	153.5
79	94.0
80	62.5
81	45.5
82	39.0
83	32.0
84	21.0
85	12.0
86	6.5
87	4.0
88	2.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.019522315577237298
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.181216190879334E-4
26-27	1.8890963249221818E-4
28-29	0.002814835915953575
30-31	0.00513832295694155
32-33	0.00329422456549178
34-35	0.0016122653941052574
36-37	0.0
38-39	0.0
40-41	3.2633937071367263E-4
42-43	0.0
44-45	0.0
46-47	2.4043776399151776E-4
48-49	0.0012869283835957983
50-51	0.0025534517241225223
52-53	0.0
54-55	0.0027444096699166674
56-57	0.03791632781672555
58-59	0.06047044023470007
60-61	0.040804232521862785
62-63	0.013493692768664717
64-65	3.421812273903754E-4
66-67	0.0
68-69	2.36084695856808E-5
70-71	0.0027719616799196565
72-73	0.005058650187200827
74-75	0.004442951015531661
76-77	0.003207768687422565
78-79	0.008091083206486098
80-81	0.01628105450266398
82-83	0.04324729655577333
84-85	0.01988748500706065
86-87	0.0021696660191833084
88-89	0.0014140416218437917
90-91	1.499454743729553E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	41.0
20-21	150.0
22-23	351.0
24-25	523.0
26-27	1402.0
28-29	3810.0
30-31	11824.0
32-33	14618.0
34-35	19861.0
36-37	31147.0
38-39	35173.0
40-41	34210.0
42-43	38436.0
44-45	44182.0
46-47	43939.0
48-49	41360.0
50-51	37308.0
52-53	35060.0
54-55	37841.0
56-57	40518.0
58-59	45206.0
60-61	59764.0
62-63	55748.0
64-65	65555.0
66-67	77325.0
68-69	75493.0
70-71	104373.0
72-73	46975.0
74-75	42321.0
76-77	44088.0
78-79	45349.0
80-81	44749.0
82-83	42267.0
84-85	41906.0
86-87	43542.0
88-89	44542.0
90-91	44384.0
92-93	43825.0
94-95	50560.0
96-97	145209.0
98-99	1060931.0
100-101	2334275.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.54646702445297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.5614777249876	81.07445368085799
2	6.266746360419419	11.097964999069774
3	1.2997790950976438	3.452725403494105
4	0.4479984669952678	1.5867472593920784
5	0.18487058182124053	0.8184818438512955
6	0.08909893351018149	0.4733637466783927
7	0.048709758244385774	0.30191539015089064
8	0.02761741214113281	0.19563394187644348
9	0.021127918021922026	0.16837222467811178
>10	0.05086671731012356	0.6503808447306314
>50	9.476267606109093E-4	0.058535030192942164
>100	7.594046904447803E-4	0.12142563502737722
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.976031685791711E-5	0.0	0.0	0.0
3	0.0	3.976031685791711E-5	0.0	0.0	0.0
4	0.0	5.964047528687566E-5	0.0	0.0	0.0
5	0.0	5.964047528687566E-5	0.0	0.0	0.0
6	1.9880158428958554E-5	5.964047528687566E-5	0.0	0.0	0.0
7	1.9880158428958554E-5	5.964047528687566E-5	0.0	3.976031685791711E-5	0.0
8	1.9880158428958554E-5	9.940079214479276E-5	0.0	3.976031685791711E-5	0.0
9	1.9880158428958554E-5	1.5904126743166843E-4	0.0	9.940079214479276E-5	3.976031685791711E-5
10-11	1.9880158428958554E-5	1.5904126743166843E-4	0.0	9.940079214479276E-5	3.976031685791711E-5
12-13	1.9880158428958554E-5	1.5904126743166843E-4	0.0	1.0934087135927204E-4	3.976031685791711E-5
14-15	2.982023764343783E-5	1.5904126743166843E-4	0.0	1.1928095057375131E-4	5.964047528687566E-5
16-17	5.964047528687566E-5	1.5904126743166843E-4	0.0	1.3916110900270986E-4	7.952063371583421E-5
18-19	5.964047528687566E-5	1.7892142586062697E-4	0.0	1.5904126743166843E-4	7.952063371583421E-5
20-21	5.964047528687566E-5	1.9880158428958552E-4	0.0	1.8886150507510624E-4	9.940079214479276E-5
22-23	5.964047528687566E-5	2.783222180054197E-4	0.0	2.0874166350406481E-4	9.940079214479276E-5
24-25	5.964047528687566E-5	3.180825348633368E-4	0.0	2.783222180054197E-4	9.940079214479276E-5
26-27	5.964047528687566E-5	3.379626932922954E-4	0.0	3.8766308936469176E-4	9.940079214479276E-5
28-29	5.964047528687566E-5	3.379626932922954E-4	0.0	7.156857034425078E-4	9.940079214479276E-5
30-31	5.964047528687566E-5	4.075432477936503E-4	0.0	0.0026142408334080495	1.0934087135927204E-4
32-33	5.964047528687566E-5	4.174833270081296E-4	0.0	0.0064312312517680915	1.1928095057375131E-4
34-35	5.964047528687566E-5	4.6718372308052593E-4	0.0	0.01085456650221137	1.1928095057375131E-4
36-37	5.964047528687566E-5	5.367642775818809E-4	0.0	0.017951783061349574	1.1928095057375131E-4
38-39	5.964047528687566E-5	5.566444360108394E-4	0.0	0.03391555027980329	1.1928095057375131E-4
40-41	5.964047528687566E-5	5.76524594439798E-4	0.0	0.05085344526127597	1.1928095057375131E-4
42-43	5.964047528687566E-5	5.964047528687565E-4	0.0	0.06196645382306381	1.2922102978823059E-4
44-45	5.964047528687566E-5	6.659853073701115E-4	0.0	0.07513705878224886	1.3916110900270986E-4
46-47	5.964047528687566E-5	7.156857034425079E-4	0.0	0.08861580619708274	1.5904126743166843E-4
48-49	5.964047528687566E-5	7.156857034425079E-4	0.0	0.101209886561828	1.5904126743166843E-4
50-51	5.964047528687566E-5	7.455059410859458E-4	0.0	0.11359522526306917	1.5904126743166843E-4
52-53	5.964047528687566E-5	7.55446020300425E-4	0.0	0.14285881847049614	1.5904126743166843E-4
54-55	5.964047528687566E-5	7.753261787293835E-4	0.0	0.1786629838010505	1.5904126743166843E-4
56-57	5.964047528687566E-5	7.753261787293835E-4	0.0	0.22401956525671946	1.689813466461477E-4
58-59	5.964047528687566E-5	7.753261787293835E-4	0.0	0.2605294762115018	1.8886150507510624E-4
60-61	5.964047528687566E-5	7.753261787293835E-4	0.0	0.2722786498430163	1.9880158428958552E-4
62-63	5.964047528687566E-5	7.753261787293835E-4	0.0	0.28617488058485835	2.1868174271854409E-4
64-65	5.964047528687566E-5	8.150864955873006E-4	0.0	0.29337149793614137	2.2862182193302336E-4
66-67	5.964047528687566E-5	8.250265748017799E-4	0.0	0.2997828490294805	2.3856190114750263E-4
68-69	5.964047528687566E-5	8.64786891659697E-4	0.0	0.3056077354491653	2.3856190114750263E-4
70-71	5.964047528687566E-5	9.045472085176141E-4	0.0	0.31082627703676696	2.3856190114750263E-4
72-73	7.952063371583421E-5	9.144872877320934E-4	0.0	0.31522973212878125	2.9820237643437826E-4
74-75	7.952063371583421E-5	9.443075253755312E-4	0.0	0.31832109676448433	2.9820237643437826E-4
76-77	7.952063371583421E-5	0.001003948000662407	0.0	0.32143234155861633	2.9820237643437826E-4
78-79	7.952063371583421E-5	0.0010337682383058447	0.0	0.3236986796195176	2.9820237643437826E-4
80-81	7.952063371583421E-5	0.0010337682383058447	0.0	0.32453364627353387	3.081424556488576E-4
82-83	7.952063371583421E-5	0.001043708317520324	0.0	0.3254381934820515	3.1808253486333686E-4
84-85	7.952063371583421E-5	0.0010536483967348033	0.0	0.3257860962545583	3.1808253486333686E-4
86-87	7.952063371583421E-5	0.0010735285551637618	0.0	0.325905377205132	3.1808253486333686E-4
88	7.952063371583421E-5	0.0011729293473085546	0.0	0.32593519744277544	3.1808253486333686E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	12580	0.0	57.61831	1
GGTATCA	6020	0.0	50.43433	1
TATACAC	15765	0.0	46.972683	3
TTATACA	15660	0.0	46.871872	2
GTATCAA	10485	0.0	34.167732	1
TACACAT	22515	0.0	33.14954	5
ATACACA	24795	0.0	30.85804	4
ACACATC	24280	0.0	30.035685	6
TCAACGC	12200	0.0	28.845564	4
ATCAACG	12295	0.0	28.62268	3
CACATCT	25935	0.0	28.070776	7
CAACGCA	12555	0.0	27.963516	5
TATCAAC	12895	0.0	27.523243	2
AACGCAG	13080	0.0	26.904882	6
CATCTCC	28045	0.0	26.077778	9
ACGCAGA	14935	0.0	23.591085	7
CGCAGAG	15125	0.0	23.267164	8
GTGGTAT	2790	0.0	20.628437	1
TGGTATC	2615	0.0	19.61665	2
GAGTACT	10020	0.0	19.599709	12-13
>>END_MODULE