FastQCFastQC Report
Fri 27 May 2016
SRR2140139_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140139_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6483460
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC91850.057.481621
GGTATCA79150.049.230651
TATACAC127000.043.7214163
TTATACA129500.041.8278772
GTATCAA141400.036.5513081
ATCAACG164250.030.938793
TCAACGC164750.030.8968664
TACACAT183500.030.6327975
TATCAAC171000.029.9238112
CAACGCA170300.029.8648095
AACGCAG174350.029.220196
ACACATC188450.028.3742436
ACATCTC196050.027.5581788
ATACACA218600.026.8108924
CACATCT206050.025.9714017
ACGCAGA203150.025.056657
CGCAGAG203800.024.9767348
CATCTCC221000.024.3307429
GTGGTAT38350.021.4378361
GCAGAGT243650.020.8389729
Produced by FastQC (version 0.11.5)