FastQCFastQC Report
Fri 27 May 2016
SRR2140138_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140138_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6345698
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC127050.062.8809431
TATACAC167050.049.657263
GGTATCA79000.048.856661
TTATACA166600.048.484452
GTATCAA140850.035.5697171
TACACAT247950.033.7441645
ATACACA276900.031.0682164
ACACATC263700.030.8819896
TCAACGC163850.029.928854
ACATCTC276400.029.6154448
ATCAACG166050.029.5830673
CAACGCA169100.028.9996595
TATCAAC171550.028.8112872
CACATCT286550.028.3311927
AACGCAG175400.028.0541296
CATCTCC305800.026.561539
ACGCAGA200500.024.4790787
CGCAGAG204600.023.988548
GTGGTAT40250.021.7747331
TGGTATC37500.021.2366452
Produced by FastQC (version 0.11.5)