##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140138_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6345698
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.51202342122175	32.0	28.0	33.0	18.0	34.0
2	31.06722128913163	33.0	30.0	33.0	27.0	34.0
3	32.34254245947412	33.0	33.0	33.0	30.0	34.0
4	32.44815495474257	33.0	33.0	34.0	31.0	34.0
5	32.92745195248813	33.0	33.0	34.0	32.0	34.0
6	36.74476472091801	38.0	37.0	38.0	34.0	38.0
7	36.970980970099745	38.0	38.0	38.0	35.0	38.0
8	37.16132141806938	38.0	38.0	38.0	36.0	38.0
9	37.2101595443086	38.0	38.0	38.0	36.0	38.0
10-11	37.28044700519943	38.0	38.0	38.0	36.5	38.0
12-13	37.32320589476524	38.0	38.0	38.0	37.0	38.0
14-15	37.32429647613233	38.0	38.0	38.0	37.0	38.0
16-17	37.36347924215744	38.0	38.0	38.0	37.0	38.0
18-19	37.36603656524467	38.0	38.0	38.0	37.0	38.0
20-21	37.383794350934345	38.0	38.0	38.0	37.0	38.0
22-23	37.315397198002145	38.0	38.0	38.0	37.0	38.0
24-25	37.37894263330029	38.0	38.0	38.0	37.0	38.0
26-27	37.34119037679865	38.0	38.0	38.0	37.0	38.0
28-29	37.32343975603931	38.0	38.0	38.0	37.0	38.0
30-31	37.32177546983211	38.0	38.0	38.0	37.0	38.0
32-33	37.30906130699368	38.0	38.0	38.0	37.0	38.0
34-35	37.21680239354703	38.0	38.0	38.0	37.0	38.0
36-37	37.237760708513264	38.0	38.0	38.0	37.0	38.0
38-39	37.19467542942933	38.0	38.0	38.0	37.0	38.0
40-41	37.27606003165903	38.0	38.0	38.0	37.0	38.0
42-43	37.262613471078154	38.0	38.0	38.0	37.0	38.0
44-45	37.228716518866406	38.0	38.0	38.0	37.0	38.0
46-47	37.1959882409959	38.0	38.0	38.0	37.0	38.0
48-49	37.141124787666215	38.0	38.0	38.0	36.5	38.0
50-51	37.10319456076599	38.0	38.0	38.0	36.0	38.0
52-53	37.011574268893895	38.0	38.0	38.0	36.0	38.0
54-55	37.04496246855197	38.0	38.0	38.0	36.0	38.0
56-57	37.011633437677816	38.0	38.0	38.0	36.0	38.0
58-59	36.979046407666054	38.0	38.0	38.0	36.0	38.0
60-61	36.95917414002503	38.0	38.0	38.0	36.0	38.0
62-63	36.97540743945783	38.0	38.0	38.0	36.0	38.0
64-65	36.984002788139506	38.0	38.0	38.0	36.0	38.0
66-67	36.97327628838984	38.0	38.0	38.0	36.0	38.0
68-69	36.9495350741642	38.0	38.0	38.0	36.0	38.0
70-71	36.95924773447394	38.0	38.0	38.0	36.0	38.0
72-73	36.97060057543183	38.0	38.0	38.0	36.0	38.0
74-75	36.927477203872904	38.0	38.0	38.0	36.0	38.0
76-77	36.944086638300405	38.0	38.0	38.0	36.0	38.0
78-79	36.95698552238031	38.0	38.0	38.0	36.0	38.0
80-81	36.94991598738264	38.0	38.0	38.0	36.0	38.0
82-83	36.91339928546654	38.0	38.0	38.0	36.0	38.0
84-85	36.912233668936054	38.0	38.0	38.0	36.0	38.0
86-87	36.917013404117455	38.0	38.0	38.0	36.0	38.0
88-89	36.90439819340972	38.0	38.0	38.0	35.0	38.0
90-91	36.917123381102115	38.0	38.0	38.0	35.5	38.0
92-93	36.90275959069007	38.0	38.0	38.0	35.0	38.0
94-95	36.842758981963584	38.0	38.0	38.0	35.0	38.0
96-97	36.87504210938675	38.0	38.0	38.0	35.0	38.0
98-99	36.90316138965134	38.0	38.0	38.0	35.0	38.0
100	35.488776835811116	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	11.0
19	22.0
20	55.0
21	149.0
22	470.0
23	1275.0
24	3288.0
25	6421.0
26	11663.0
27	19119.0
28	29557.0
29	42043.0
30	56514.0
31	72347.0
32	93024.0
33	123940.0
34	179916.0
35	315519.0
36	883070.0
37	4507295.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.15983710539014	21.949311171127274	14.184081877202475	24.70676984628011
2	14.279891452114049	20.27165027367881	43.437989648010614	22.010468626196534
3	17.587080255001105	27.049758749943663	31.07883482636583	24.2843261686894
4	12.358451347668925	19.205688641344103	37.878654168540635	30.557205842446333
5	13.075110098211418	37.69861093295017	36.24346762168638	12.98281134715204
6	28.84138198823833	38.35234516360533	18.633805138536374	14.172467709619966
7	25.493586363548975	32.99994736591625	22.329489994638887	19.17697627589589
8	23.436807109320362	37.66034879062949	21.638502178956514	17.264341921093628
9	24.530682046324927	17.481244774018556	20.691009877873167	37.29706330178335
10-11	23.725278133311733	27.051318862006983	29.040201724065657	20.183201280615624
12-13	24.923452077297092	24.008083901881243	28.817121457718287	22.251342563103382
14-15	21.96342309388187	25.797091194695998	25.760389164438646	26.479096546983484
16-17	21.914689290287686	29.539185445005415	26.357786015029394	22.188339249677497
18-19	22.145861653044314	27.909151995572433	28.114519474453402	21.83046687692985
20-21	22.59843668718631	27.00485704564734	28.07645328034566	22.320252986820687
22-23	22.172151928657122	27.886957703695387	27.98963818349083	21.951252184156665
24-25	22.670248615299364	27.819569017221298	28.167884653709983	21.342297713769355
26-27	22.71256582711644	27.822034686417823	28.11945267146575	21.34594681499999
28-29	21.94530807374652	27.25788844357031	28.476624138628654	22.320179344054512
30-31	22.111746393170716	27.37727592968212	28.952018234747275	21.558959442399892
32-33	21.07074582192677	26.911599721353674	29.765019127269955	22.252635329449603
34-35	21.22795537731077	27.18051917637031	29.00576347842202	22.585761967896897
36-37	21.56899455986345	27.516329053888843	29.46831853229206	21.446357853955643
38-39	21.597237086723442	27.073487688986265	29.267504102602338	22.061771121687958
40-41	21.830757549393358	26.940473580956812	29.472414905187282	21.756353964462544
42-43	22.4577502507644	26.737500551409525	29.25877642896712	21.545972768858956
44-45	21.603481908173432	27.479553939862267	29.166138000980656	21.750826150983645
46-47	22.581815881689803	26.643799056581024	28.354063865917052	22.42032119581212
48-49	22.20551672784426	26.71557542334977	28.47719021191851	22.601717636887464
50-51	21.580372407543123	26.932191252861614	29.23967863748284	22.24775770211242
52-53	21.859754834005894	27.093650432504074	29.791533628069335	21.2550611054207
54-55	21.747601636237363	27.274618817513556	28.83820211565024	22.139577430598838
56-57	22.52829231717634	28.07820479603485	27.73011325173857	21.663389635050244
58-59	21.333450115543346	29.156983186307308	28.80492987262942	20.704636825519927
60-61	22.059731050641915	30.260127321463848	27.067540872800596	20.61260075509364
62-63	21.177884700180254	31.1408106897318	27.01413115945352	20.667173450634422
64-65	21.224109987215556	30.90126134728527	27.120205677818838	20.754422987680332
66-67	21.477216914658285	30.605499390062977	27.06975474734843	20.847528947930304
68-69	21.59767578944579	29.87702266920435	27.454847574885417	21.07045396646444
70-71	21.84636422213341	28.845706187819438	27.823901430041033	21.48402816000612
72-73	22.1828955456312	28.245397570118445	27.982297835778947	21.58940904847141
74-75	21.962256247716837	28.262587725245712	28.053538277716306	21.721617749321144
76-77	22.09530013073243	28.05539921809947	28.01403325274147	21.835267398426634
78-79	22.068203579728067	28.049829383305198	28.039113873593784	21.84285316337295
80-81	22.135514966835196	27.989969586461168	27.9952285548663	21.879286891837335
82-83	22.130002771539274	27.95072747632112	28.11717051455902	21.802099237580585
84-85	22.25974815051265	27.89735104374178	28.061710486049275	21.781190319696304
86-87	22.02404133399346	27.914563336597105	28.107645863304125	21.953749466105315
88-89	22.131588105741677	27.908938172216775	28.068447368325796	21.891026353715752
90-91	22.190752215511587	27.901391113910357	28.09885977268629	21.80899689789177
92-93	22.204242470095306	27.91460070931848	28.015131873979527	21.86602494660669
94-95	22.102725979472755	28.037656968585186	27.980999502657923	21.878617549284144
96-97	22.14464539024791	28.10287737483733	28.122950013920644	21.629527220994113
98-99	23.19426148118906	30.00881697796997	28.350899562453513	18.446021978387456
100	29.37933407052463	41.00638080026211	29.614285129213254	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1180.0
1	1030.0
2	1112.5
3	1509.5
4	2008.5
5	2874.0
6	3646.5
7	4532.5
8	5723.0
9	6575.5
10	6667.0
11	6748.5
12	7663.5
13	9510.0
14	13220.0
15	17792.5
16	21356.0
17	23308.5
18	23565.0
19	22488.0
20	20721.0
21	19537.5
22	20386.0
23	23770.0
24	30076.0
25	39460.5
26	51839.5
27	65308.0
28	78305.0
29	92463.0
30	106312.5
31	120322.5
32	135858.5
33	151717.5
34	167333.5
35	181657.0
36	197491.0
37	212020.0
38	222294.5
39	231206.5
40	242775.0
41	254059.5
42	260152.0
43	266384.0
44	291824.0
45	337769.5
46	344315.0
47	322526.0
48	301814.5
49	271413.5
50	251827.0
51	238856.0
52	224010.0
53	208855.0
54	192905.0
55	175752.5
56	158773.0
57	141444.5
58	124749.5
59	107257.5
60	89016.0
61	71553.0
62	55690.0
63	42887.5
64	32203.0
65	23650.0
66	17481.5
67	13313.0
68	10075.0
69	7330.5
70	5381.0
71	3898.5
72	2764.0
73	1917.0
74	1301.0
75	877.5
76	564.0
77	346.5
78	231.0
79	165.0
80	103.0
81	62.5
82	46.5
83	31.5
84	18.5
85	10.5
86	7.5
87	6.0
88	2.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.019178347283466686
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.122146649107423E-4
26-27	4.255790673103734E-4
28-29	0.0035635429374599793
30-31	0.005816809949349555
32-33	0.004303233009467587
34-35	0.0020547162202868403
36-37	0.0
38-39	8.016809003774393E-6
40-41	4.1160290843457486E-4
42-43	8.123896571748142E-6
44-45	8.185790597162429E-6
46-47	2.559862170414649E-4
48-49	0.0015491975156868743
50-51	0.002795736443154954
52-53	0.0
54-55	0.00284575666582288
56-57	0.03912350972574729
58-59	0.06139423174742391
60-61	0.04220947427588874
62-63	0.014108003823269037
64-65	4.01127900344141E-4
66-67	0.0
68-69	0.0
70-71	0.0031405409905285037
72-73	0.004911081243329075
74-75	0.0041981852258623645
76-77	0.003185642901705673
78-79	0.008028607818742373
80-81	0.016688722816714487
82-83	0.04287567450113349
84-85	0.01974884097215393
86-87	0.0019144083387941746
88-89	0.001354822122197509
90-91	9.408238522680754E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	37.0
20-21	165.0
22-23	413.0
24-25	548.0
26-27	1621.0
28-29	4600.0
30-31	13643.0
32-33	16912.0
34-35	23335.0
36-37	36715.0
38-39	42546.0
40-41	40097.0
42-43	44321.0
44-45	52132.0
46-47	52996.0
48-49	48949.0
50-51	44569.0
52-53	42745.0
54-55	45003.0
56-57	47251.0
58-59	48707.0
60-61	53143.0
62-63	59649.0
64-65	77939.0
66-67	91071.0
68-69	87380.0
70-71	120994.0
72-73	55662.0
74-75	47878.0
76-77	50040.0
78-79	51276.0
80-81	51698.0
82-83	47997.0
84-85	47519.0
86-87	48972.0
88-89	51121.0
90-91	51719.0
92-93	50858.0
94-95	58594.0
96-97	184052.0
98-99	1398706.0
100-101	3052125.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.56363250856973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.95240644286659	79.64123093533239
2	6.62906852167831	11.609306398127341
3	1.4358974090098615	3.7719717912764086
4	0.4918052850124319	1.7225702897040391
5	0.2051380017141462	0.8981314297819922
6	0.10305339990811242	0.5414238022987578
7	0.05793479715968712	0.355108690156456
8	0.035148115021953655	0.24621573017210313
9	0.022349885241042984	0.17613334240698547
>10	0.06527129818735619	0.8293311017906474
>50	0.0012842030182716008	0.07231892280875718
>100	5.909043121493377E-4	0.0993585311305177
>500	5.173687010227669E-5	0.0368990350135668
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5758707710325958E-5
2	0.0	1.5758707710325958E-5	0.0	0.0	1.5758707710325958E-5
3	0.0	1.5758707710325958E-5	0.0	0.0	1.5758707710325958E-5
4	0.0	1.5758707710325958E-5	0.0	0.0	1.5758707710325958E-5
5	0.0	1.5758707710325958E-5	0.0	0.0	1.5758707710325958E-5
6	0.0	3.1517415420651915E-5	0.0	0.0	1.5758707710325958E-5
7	0.0	4.7276123130977866E-5	0.0	0.0	1.5758707710325958E-5
8	0.0	7.879353855162978E-5	0.0	0.0	1.5758707710325958E-5
9	0.0	1.5758707710325956E-4	0.0	1.5758707710325958E-5	1.5758707710325958E-5
10-11	0.0	1.5758707710325956E-4	0.0	3.1517415420651915E-5	1.5758707710325958E-5
12-13	0.0	1.5758707710325956E-4	0.0	3.1517415420651915E-5	1.5758707710325958E-5
14-15	0.0	1.5758707710325956E-4	0.0	4.727612313097787E-5	3.1517415420651915E-5
16-17	0.0	1.5758707710325956E-4	0.0	7.879353855162978E-5	4.7276123130977866E-5
18-19	0.0	1.5758707710325956E-4	0.0	8.667289240679276E-5	4.7276123130977866E-5
20-21	0.0	1.5758707710325956E-4	0.0	1.103109539722817E-4	6.303483084130383E-5
22-23	0.0	1.8910449252391146E-4	0.0	1.418283693929336E-4	7.879353855162978E-5
24-25	0.0	2.206219079445634E-4	0.0	1.4970772324809657E-4	9.455224626195573E-5
26-27	0.0	2.521393233652153E-4	0.0	1.7334578481358553E-4	9.455224626195573E-5
28-29	0.0	2.521393233652153E-4	0.0	4.176057543236378E-4	9.455224626195573E-5
30-31	0.0	2.994154464961932E-4	0.0	0.001473439170915477	9.455224626195573E-5
32-33	0.0	2.994154464961932E-4	0.0	0.0037190550196369255	9.455224626195573E-5
34-35	0.0	3.62450277337497E-4	0.0	0.006358638561116523	1.103109539722817E-4
36-37	0.0	4.333644620339638E-4	0.0	0.010180125180870568	1.103109539722817E-4
38-39	0.0	4.412438158891268E-4	0.0	0.015908415433574054	1.103109539722817E-4
40-41	0.0	4.885199390201046E-4	0.0	0.021526394732305255	1.103109539722817E-4
42-43	0.0	5.200373544407566E-4	0.0	0.02712861532332613	1.103109539722817E-4
44-45	0.0	6.697450776888532E-4	0.0	0.03299085459156739	1.103109539722817E-4
46-47	0.0	7.879353855162978E-4	0.0	0.03928645832184261	1.103109539722817E-4
48-49	0.0	7.879353855162978E-4	0.0	0.04569237300609011	1.103109539722817E-4
50-51	0.0	8.430908625024386E-4	0.0	0.051712199351434625	1.2606966168260766E-4
52-53	0.0	8.509702163576016E-4	0.0	0.05737745477329681	1.2606966168260766E-4
54-55	0.0	8.509702163576016E-4	0.0	0.06349183336490327	1.3394901553777063E-4
56-57	0.0	8.982463394885795E-4	0.0	0.06962197066422007	1.418283693929336E-4
58-59	0.0	8.982463394885795E-4	0.0	0.07543693380933035	1.418283693929336E-4
60-61	0.0	8.982463394885795E-4	0.0	0.0808736879693928	1.418283693929336E-4
62-63	0.0	9.218844010540684E-4	0.0	0.08611345828307619	1.5758707710325956E-4
64-65	0.0	0.001071592124302165	0.0	0.0912192795812218	1.5758707710325956E-4
66-67	0.0	0.001087350832012491	0.0	0.09608084090985736	1.5758707710325956E-4
68-69	0.0	0.0011188682474331428	0.0	0.1011787828541478	1.5758707710325956E-4
70-71	0.0	0.0011425063089986318	0.0	0.10555182424376325	1.7334578481358553E-4
72-73	0.0	0.0011503856628537947	0.0	0.10945998375592408	1.8910449252391146E-4
74-75	0.0	0.0011661443705641207	0.0	0.11239898274389988	1.8910449252391146E-4
76-77	0.0	0.0012528172629709135	0.0	0.11510160111622078	1.9698384637907443E-4
78-79	0.0	0.0012922140322467284	0.0	0.11703992216459089	2.0486320023423743E-4
80-81	0.0	0.0012922140322467284	0.0	0.11767027047300391	2.0486320023423743E-4
82-83	0.0	0.0013158520938122173	0.0	0.11832425684298245	2.0486320023423743E-4
84-85	0.0	0.0013473695092328692	0.0	0.11854487875092701	2.0486320023423743E-4
86-87	0.0	0.0013552488630880323	0.0	0.11866306905875446	2.0486320023423743E-4
88	0.0	0.0014025249862190101	0.0	0.11867882776646478	2.0486320023423743E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	12705	0.0	62.880943	1
TATACAC	16705	0.0	49.65726	3
GGTATCA	7900	0.0	48.85666	1
TTATACA	16660	0.0	48.48445	2
GTATCAA	14085	0.0	35.569717	1
TACACAT	24795	0.0	33.744164	5
ATACACA	27690	0.0	31.068216	4
ACACATC	26370	0.0	30.881989	6
TCAACGC	16385	0.0	29.92885	4
ACATCTC	27640	0.0	29.615444	8
ATCAACG	16605	0.0	29.583067	3
CAACGCA	16910	0.0	28.999659	5
TATCAAC	17155	0.0	28.811287	2
CACATCT	28655	0.0	28.331192	7
AACGCAG	17540	0.0	28.054129	6
CATCTCC	30580	0.0	26.56153	9
ACGCAGA	20050	0.0	24.479078	7
CGCAGAG	20460	0.0	23.98854	8
GTGGTAT	4025	0.0	21.774733	1
TGGTATC	3750	0.0	21.236645	2
>>END_MODULE