FastQCFastQC Report
Fri 27 May 2016
SRR2140137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5472175
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC101700.060.8884851
GGTATCA71900.050.8998221
TTATACA132950.047.1163442
TATACAC136250.046.708473
GTATCAA129300.034.931021
TACACAT199950.031.8914185
ACACATC211300.029.6990286
ATCAACG150750.029.5062543
TCAACGC150800.029.4684854
ATACACA232100.028.910314
CAACGCA154650.028.7348695
TATCAAC157500.028.4891012
ACATCTC222850.028.1976458
AACGCAG159950.027.7827286
CACATCT232100.026.9647737
TGGTATC31000.026.143612
GTGGTAT33750.025.3891561
CATCTCC249450.025.1062099
ACGCAGA181900.024.4765687
CGCAGAG183900.024.1644788
Produced by FastQC (version 0.11.5)