FastQCFastQC Report
Fri 27 May 2016
SRR2140136_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140136_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6330092
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC121300.061.142251
GGTATCA86150.050.857291
TATACAC157000.049.1677253
TTATACA157450.047.9107782
GTATCAA161050.039.006891
TCAACGC194450.031.9719244
ATCAACG195950.031.7271773
TACACAT247250.031.3748075
CAACGCA198500.031.31965
TATCAAC202650.030.9159972
AACGCAG204850.030.3900666
ATACACA270850.029.4535354
ACACATC269000.028.0828296
ACATCTC281150.027.0046968
ACGCAGA235600.026.3876937
CACATCT289800.025.9796037
CGCAGAG239800.025.9078758
CATCTCC307850.024.4288589
TGGTATC38550.023.9386832
GTGGTAT41550.023.7384851
Produced by FastQC (version 0.11.5)