##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140136_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6330092
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.542547406893927	32.0	28.0	33.0	18.0	34.0
2	31.0426872152885	33.0	30.0	33.0	27.0	34.0
3	32.335305079294265	33.0	33.0	33.0	30.0	34.0
4	32.42659048241321	33.0	33.0	34.0	31.0	34.0
5	32.91123035810538	33.0	33.0	34.0	32.0	34.0
6	36.72476576959703	38.0	37.0	38.0	34.0	38.0
7	36.93300839861411	38.0	38.0	38.0	35.0	38.0
8	37.140465099085446	38.0	38.0	38.0	36.0	38.0
9	37.189976227833654	38.0	38.0	38.0	36.0	38.0
10-11	37.26727778995945	38.0	38.0	38.0	36.5	38.0
12-13	37.312585425298714	38.0	38.0	38.0	36.5	38.0
14-15	37.315649835736984	38.0	38.0	38.0	37.0	38.0
16-17	37.3610791912661	38.0	38.0	38.0	37.0	38.0
18-19	37.36385205775841	38.0	38.0	38.0	37.0	38.0
20-21	37.38402465799844	38.0	38.0	38.0	37.0	38.0
22-23	37.31949786514555	38.0	38.0	38.0	37.0	38.0
24-25	37.386768633806554	38.0	38.0	38.0	37.0	38.0
26-27	37.35048732138041	38.0	38.0	38.0	37.0	38.0
28-29	37.32282558209552	38.0	38.0	38.0	37.0	38.0
30-31	37.32418315975494	38.0	38.0	38.0	37.0	38.0
32-33	37.29159992844585	38.0	38.0	38.0	37.0	38.0
34-35	37.18308729393198	38.0	38.0	38.0	36.5	38.0
36-37	37.205442411398884	38.0	38.0	38.0	36.5	38.0
38-39	37.16448608182306	38.0	38.0	38.0	36.0	38.0
40-41	37.26003127659863	38.0	38.0	38.0	37.0	38.0
42-43	37.2475671972295	38.0	38.0	38.0	37.0	38.0
44-45	37.209772253273286	38.0	38.0	38.0	37.0	38.0
46-47	37.1756790087292	38.0	38.0	38.0	37.0	38.0
48-49	37.11840230610788	38.0	38.0	38.0	36.5	38.0
50-51	37.08303555619139	38.0	38.0	38.0	36.0	38.0
52-53	36.99306963619193	38.0	38.0	38.0	36.0	38.0
54-55	37.029310724373346	38.0	38.0	38.0	36.0	38.0
56-57	36.99893618860969	38.0	38.0	38.0	36.0	38.0
58-59	36.96827095752418	38.0	38.0	38.0	36.0	38.0
60-61	36.95576997464469	38.0	38.0	38.0	36.0	38.0
62-63	36.969593610681386	38.0	38.0	38.0	36.0	38.0
64-65	36.98013854647647	38.0	38.0	38.0	36.0	38.0
66-67	36.96808975944055	38.0	38.0	38.0	36.0	38.0
68-69	36.94505596267622	38.0	38.0	38.0	36.0	38.0
70-71	36.95089432359889	38.0	38.0	38.0	36.0	38.0
72-73	36.96213207813787	38.0	38.0	38.0	36.0	38.0
74-75	36.91939571154458	38.0	38.0	38.0	36.0	38.0
76-77	36.93973642981001	38.0	38.0	38.0	36.0	38.0
78-79	36.957153866114496	38.0	38.0	38.0	36.0	38.0
80-81	36.95038116673102	38.0	38.0	38.0	36.0	38.0
82-83	36.91649482825042	38.0	38.0	38.0	36.0	38.0
84-85	36.918984656492086	38.0	38.0	38.0	36.0	38.0
86-87	36.92155285005306	38.0	38.0	38.0	36.0	38.0
88-89	36.914287913851894	38.0	38.0	38.0	35.5	38.0
90-91	36.92814404111087	38.0	38.0	38.0	36.0	38.0
92-93	36.91221852208393	38.0	38.0	38.0	35.0	38.0
94-95	36.858375635589226	38.0	38.0	38.0	35.0	38.0
96-97	36.89406386248381	38.0	38.0	38.0	35.0	38.0
98-99	36.92340786300173	38.0	38.0	38.0	35.0	38.0
100	35.48571009167454	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	10.0
19	35.0
20	60.0
21	163.0
22	538.0
23	1369.0
24	3202.0
25	6700.0
26	11616.0
27	19186.0
28	29815.0
29	42927.0
30	57897.0
31	73923.0
32	94352.0
33	124380.0
34	181861.0
35	320754.0
36	889763.0
37	4471540.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.32309356641262	22.08699336439344	14.268844749807744	24.321068319386196
2	14.167590202337262	20.419097169224916	43.39287254828535	22.02044008015247
3	17.440947145791878	27.417279243334853	31.17368594326907	23.9680876676042
4	12.186647524238195	19.245249516120776	38.0650233835464	30.503079576094628
5	12.862103741936137	37.92832710804203	36.15713642076608	13.052432729255752
6	28.420503209116077	38.33580933736825	18.948602958693176	14.295084494822508
7	25.27291862424748	33.3191049987899	22.432249009966995	18.975727366995613
8	23.498141891144712	37.30318611483056	21.83736034168224	17.361311652342494
9	24.472487919606856	17.43666600738188	20.790424530954684	37.300421542056576
10-11	23.545668846519135	26.99295681642542	29.13206474724222	20.32930958981323
12-13	24.89371402500943	24.150359899982497	28.904990638366712	22.050935436641364
14-15	21.95024179743359	25.881717042975044	26.010704109829685	26.157337049761676
16-17	21.674092572430226	29.58417507991985	26.580221898828643	22.161510448821282
18-19	21.80413807571833	27.977215812977125	28.50601539440501	21.712630716899533
20-21	22.47327439676191	27.017758459266872	28.42216038466196	22.086806759309262
22-23	22.04345611926297	27.928956167013215	28.30760889287286	21.719978820850955
24-25	22.311618763530706	28.186295517590466	28.424961321083003	21.077124397795824
26-27	22.410019361107164	28.2195918982623	28.38354427559548	20.986844465035055
28-29	21.75248522962371	28.109311217392623	28.512097987789147	21.62610556519452
30-31	23.048815098342835	27.738393627228707	28.491307429754354	20.721483844674104
32-33	21.079057297188957	28.665934125354315	28.84839288739861	21.406615690058118
34-35	21.725318050540825	28.282711521179305	29.285986632432603	20.705983795847267
36-37	21.328659998531435	28.215087267863016	29.111582186948294	21.34467054665726
38-39	21.377733089649965	27.0417980981395	29.6721319897355	21.90833682247504
40-41	21.668567593249215	26.926814892083435	29.82964207806418	21.574975436603168
42-43	22.217204893104604	26.691661529640736	29.671174511939174	21.41995906531549
44-45	21.455069949285193	27.34287557762809	29.50907816527743	21.692976307809285
46-47	22.313379821313635	26.554816785394735	28.79910474282624	22.332698650465392
48-49	21.985445673873592	26.661538670385966	28.888810788180415	22.46420486756003
50-51	21.409628995178	26.972829860717727	29.511215443156914	22.106325700947362
52-53	21.660829015736724	27.120059527643015	30.013470461925788	21.205640994694473
54-55	21.641924623549706	27.323037613324487	29.046876323262744	21.98816143986307
56-57	22.285646287440077	28.18668678605029	28.02812138000806	21.499545546501565
58-59	21.10752784155467	29.290296386099087	28.964549850464522	20.637625921881725
60-61	21.776494301241485	30.35149198332566	27.346906129685888	20.52510758574697
62-63	20.979617424440868	31.085736775582994	27.309437512409207	20.62520828756693
64-65	21.00881225954764	30.87623459242799	27.427682866154335	20.687270281870035
66-67	21.26042497682335	30.47379675552222	27.44208005125407	20.823698216400363
68-69	21.436181979601045	29.705600158158774	27.83515402106922	21.023063841170963
70-71	21.71607960724199	28.756169598688313	28.185721531876023	21.342029262193673
72-73	21.98022088040429	28.176317660222377	28.329562509074336	21.513898950299
74-75	21.81202630692679	28.2113771134665	28.320236946757916	21.656359632848794
76-77	21.92981467581626	28.088673247188485	28.274747440204735	21.70676463679052
78-79	21.913502693223148	28.105791732252833	28.282929569803482	21.697776004720538
80-81	21.984876993620787	28.009418698848936	28.246224278702453	21.759480028827817
82-83	21.995924069777875	27.953787699150208	28.33986152411592	21.710426706956
84-85	22.133735062612548	27.951391889074532	28.234013166557876	21.68085988175504
86-87	21.94701103785372	27.96423599087555	28.2726319056474	21.816121065623328
88-89	22.016067692497966	27.947880486874787	28.233244354664166	21.80280746596308
90-91	22.06123056358952	27.957117903584532	28.26563665328124	21.71601487954471
92-93	22.085067976544615	27.964500775314217	28.23087756476194	21.719553683379228
94-95	21.939424475922948	28.08086368187828	28.215093197860497	21.764618644338274
96-97	22.066429139866752	28.15176592583053	28.282373974415126	21.49943095988759
98-99	23.103634160069426	30.031868207196506	28.545851000976004	18.318646631758064
100	22.01826606173205	30.95700118711698	22.479441828809275	24.545290922341696
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1084.0
1	964.5
2	1251.0
3	1837.5
4	2419.5
5	3374.0
6	4227.0
7	5308.0
8	6625.0
9	7553.0
10	7745.5
11	7759.0
12	8732.5
13	11120.0
14	15633.0
15	20745.0
16	24580.0
17	26708.0
18	26695.0
19	25101.0
20	22838.5
21	20984.5
22	21245.5
23	24308.5
24	30367.5
25	39445.0
26	51898.0
27	65659.0
28	78514.5
29	92947.5
30	107708.5
31	121504.0
32	137034.5
33	152773.0
34	168312.0
35	184315.0
36	201953.0
37	217218.0
38	225851.0
39	232994.0
40	242770.0
41	250789.0
42	258857.5
43	289902.5
44	336678.0
45	355632.5
46	332653.5
47	296908.5
48	268310.0
49	250630.0
50	241811.5
51	231595.5
52	218882.0
53	204821.5
54	190710.0
55	174994.0
56	157923.5
57	140333.0
58	122512.0
59	105813.0
60	88365.0
61	70937.0
62	55254.0
63	42000.0
64	31405.0
65	22959.0
66	16610.0
67	12329.0
68	9212.5
69	6565.0
70	4596.0
71	3282.0
72	2317.5
73	1529.5
74	969.0
75	615.0
76	396.5
77	261.5
78	172.0
79	104.5
80	68.0
81	44.5
82	30.5
83	20.5
84	13.5
85	10.5
86	8.5
87	6.5
88	5.0
89	4.0
90	3.5
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.01823038274957141
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.949835509100223E-4
26-27	2.923197874187302E-4
28-29	0.0033747526926693337
30-31	0.005340370702425683
32-33	0.003599413216363503
34-35	0.001852332233355384
36-37	0.0
38-39	1.6054330422843772E-5
40-41	3.635417987030283E-4
42-43	0.0
44-45	8.190110817932445E-6
46-47	2.477825731047379E-4
48-49	0.0012991939584149667
50-51	0.002475642821638662
52-53	0.0
54-55	0.0029533820139205575
56-57	0.037898224014952776
58-59	0.06037281770935986
60-61	0.0412126906141432
62-63	0.013152578983921897
64-65	3.55952545474495E-4
66-67	0.0
68-69	0.0
70-71	0.002882338452416335
72-73	0.0051952786070628715
74-75	0.004080874069327313
76-77	0.003069891918431806
78-79	0.007240404281413912
80-81	0.015242202864998975
82-83	0.042199464939611527
84-85	0.019207603988310947
86-87	0.0018670525922188797
88-89	0.0012367538509423033
90-91	1.353744326639762E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	47.0
20-21	156.0
22-23	425.0
24-25	531.0
26-27	1632.0
28-29	4606.0
30-31	12469.0
32-33	16066.0
34-35	21745.0
36-37	33701.0
38-39	39747.0
40-41	39207.0
42-43	42483.0
44-45	50457.0
46-47	51053.0
48-49	46906.0
50-51	42929.0
52-53	40700.0
54-55	43673.0
56-57	46039.0
58-59	46737.0
60-61	54882.0
62-63	58904.0
64-65	78333.0
66-67	91503.0
68-69	87548.0
70-71	118274.0
72-73	52607.0
74-75	47752.0
76-77	49870.0
78-79	51238.0
80-81	50582.0
82-83	47212.0
84-85	47062.0
86-87	49296.0
88-89	49531.0
90-91	50712.0
92-93	50184.0
94-95	58660.0
96-97	183550.0
98-99	404087.0
100-101	4066996.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.92690912096126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.40697883319474	78.58799232933787
2	7.002266730417604	12.173708074314831
3	1.5553549783918632	4.056066025725124
4	0.5185320910139877	1.8029756780749966
5	0.23016369708479895	1.0003709389717386
6	0.10366908524189836	0.5406979890885409
7	0.06225509888766087	0.3788150326326907
8	0.03389554388451745	0.23571478902840015
9	0.02059458957181874	0.16112016144836852
>10	0.06411521751800366	0.8192987496652374
>50	0.001351247362501262	0.07942140343862514
>100	7.697108875652276E-4	0.12829512932195683
>500	5.317654298894456E-5	0.03552369895155073
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.7392676125402285E-5	0.0
3	0.0	0.0	0.0	4.7392676125402285E-5	0.0
4	0.0	1.5797558708467428E-5	0.0	4.7392676125402285E-5	0.0
5	0.0	1.5797558708467428E-5	0.0	6.319023483386971E-5	0.0
6	0.0	1.5797558708467428E-5	0.0	6.319023483386971E-5	1.5797558708467428E-5
7	0.0	1.5797558708467428E-5	0.0	6.319023483386971E-5	1.5797558708467428E-5
8	0.0	1.5797558708467428E-5	0.0	6.319023483386971E-5	1.5797558708467428E-5
9	4.7392676125402285E-5	4.7392676125402285E-5	0.0	6.319023483386971E-5	1.5797558708467428E-5
10-11	5.5291455479636E-5	4.7392676125402285E-5	0.0	1.2638046966773943E-4	1.5797558708467428E-5
12-13	6.319023483386971E-5	4.7392676125402285E-5	0.0	1.2638046966773943E-4	1.5797558708467428E-5
14-15	6.319023483386971E-5	7.898779354233714E-5	0.0	1.2638046966773943E-4	3.1595117416934856E-5
16-17	6.319023483386971E-5	8.688657289657086E-5	0.0	1.7377314579314172E-4	6.319023483386971E-5
18-19	9.478535225080457E-5	1.1848169031350572E-4	0.0	1.8957070450160914E-4	6.319023483386971E-5
20-21	1.4217802837620684E-4	1.4217802837620687E-4	0.0	1.9746948385584285E-4	6.319023483386971E-5
22-23	1.5797558708467428E-4	1.4217802837620687E-4	0.0	2.0536826321007658E-4	8.688657289657086E-5
24-25	1.5797558708467428E-4	1.5797558708467428E-4	0.0	2.7645727739818E-4	1.3427924902197313E-4
26-27	1.5797558708467428E-4	1.5797558708467428E-4	0.0	3.3964751223204974E-4	1.5797558708467428E-4
28-29	1.5797558708467428E-4	1.5797558708467428E-4	0.0	6.713962451098657E-4	1.5797558708467428E-4
30-31	1.5797558708467428E-4	1.8957070450160914E-4	0.0	0.00193520094178726	1.5797558708467428E-4
32-33	1.5797558708467428E-4	1.8957070450160914E-4	0.0	0.00476296395060293	1.8957070450160914E-4
34-35	1.5797558708467428E-4	1.8957070450160914E-4	0.0	0.00820683174904883	2.21165821918544E-4
36-37	1.7377314579314172E-4	1.8957070450160914E-4	0.0	0.013625394386053157	2.21165821918544E-4
38-39	1.7377314579314172E-4	1.8957070450160914E-4	0.0	0.021437287167390302	2.21165821918544E-4
40-41	1.8167192514737543E-4	2.0536826321007658E-4	0.0	0.02941505431516635	2.21165821918544E-4
42-43	1.8957070450160914E-4	2.2906460127277773E-4	0.0	0.037005781274584953	2.21165821918544E-4
44-45	1.8957070450160914E-4	2.843560567524137E-4	0.0	0.045117827671382976	2.2906460127277773E-4
46-47	1.8957070450160914E-4	3.1595117416934856E-4	0.0	0.05422512026681445	2.3696338062701144E-4
48-49	1.9746948385584285E-4	3.1595117416934856E-4	0.0	0.06322972873064088	2.3696338062701144E-4
50-51	2.0536826321007658E-4	3.4754629158628345E-4	0.0	0.07206056404867417	2.3696338062701144E-4
52-53	2.0536826321007658E-4	3.4754629158628345E-4	0.0	0.08063073964801776	2.4486215998124514E-4
54-55	2.2116582191854402E-4	3.5544507094051716E-4	0.0	0.08837154341516679	2.6855849804394627E-4
56-57	2.3696338062701144E-4	3.6334385029475086E-4	0.0	0.09398757553602696	2.8435605675241374E-4
58-59	2.3696338062701144E-4	3.6334385029475086E-4	0.0	0.09957991131882443	2.8435605675241374E-4
60-61	2.3696338062701144E-4	3.6334385029475086E-4	0.0	0.10513275320485074	2.8435605675241374E-4
62-63	2.3696338062701144E-4	3.7124262964898457E-4	0.0	0.11039334025477039	3.0015361546088115E-4
64-65	2.3696338062701144E-4	4.1073652642015316E-4	0.0	0.11540116636535457	3.0015361546088115E-4
66-67	2.3696338062701144E-4	4.1073652642015316E-4	0.0	0.12003774984628976	3.0805239481511486E-4
68-69	2.3696338062701144E-4	4.42331643837088E-4	0.0	0.1244531675053064	3.3964751223204974E-4
70-71	2.3696338062701144E-4	4.42331643837088E-4	0.0	0.12837886084436056	3.6334385029475086E-4
72-73	2.3696338062701144E-4	4.42331643837088E-4	0.0	0.13205179324407923	3.949389677116857E-4
74-75	2.3696338062701144E-4	4.42331643837088E-4	0.0	0.1349269489290203	3.949389677116857E-4
76-77	2.3696338062701144E-4	4.42331643837088E-4	0.0	0.13739926686689546	4.107365264201531E-4
78-79	2.3696338062701144E-4	4.42331643837088E-4	0.0	0.13904221297257607	4.265340851286206E-4
80-81	2.3696338062701144E-4	4.42331643837088E-4	0.0	0.13981629334929097	4.5812920254555546E-4
82-83	2.3696338062701144E-4	4.42331643837088E-4	0.0	0.14032971400731617	4.739267612540229E-4
84-85	2.3696338062701144E-4	4.42331643837088E-4	0.0	0.14056667738794318	4.739267612540229E-4
86-87	2.3696338062701144E-4	4.739267612540229E-4	0.0	0.1407325517543821	4.739267612540229E-4
88	2.3696338062701144E-4	5.055218786709577E-4	0.0	0.14075624809244477	4.739267612540229E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	12130	0.0	61.14225	1
GGTATCA	8615	0.0	50.85729	1
TATACAC	15700	0.0	49.167725	3
TTATACA	15745	0.0	47.910778	2
GTATCAA	16105	0.0	39.00689	1
TCAACGC	19445	0.0	31.971924	4
ATCAACG	19595	0.0	31.727177	3
TACACAT	24725	0.0	31.374807	5
CAACGCA	19850	0.0	31.3196	5
TATCAAC	20265	0.0	30.915997	2
AACGCAG	20485	0.0	30.390066	6
ATACACA	27085	0.0	29.453535	4
ACACATC	26900	0.0	28.082829	6
ACATCTC	28115	0.0	27.004696	8
ACGCAGA	23560	0.0	26.387693	7
CACATCT	28980	0.0	25.979603	7
CGCAGAG	23980	0.0	25.907875	8
CATCTCC	30785	0.0	24.428858	9
TGGTATC	3855	0.0	23.938683	2
GTGGTAT	4155	0.0	23.738485	1
>>END_MODULE