FastQCFastQC Report
Fri 27 May 2016
SRR2140135_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140135_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5885589
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC108100.061.6977581
GGTATCA75350.048.612431
TATACAC145550.047.648563
TTATACA142350.047.418232
GTATCAA133350.035.979191
TACACAT208350.033.4287765
ATACACA235950.030.25424
ACACATC230000.029.7114476
ATCAACG158600.029.6858653
TCAACGC158600.029.6858654
CAACGCA161950.029.0456565
TATCAAC164700.028.8992942
ACATCTC240150.028.4027928
AACGCAG168550.027.933426
CACATCT249850.027.2323177
CATCTCC273300.024.7717639
ACGCAGA192350.024.4331177
CGCAGAG196800.023.9021538
GTGGTAT37300.022.9327981
TGGTATC35400.022.4889452
Produced by FastQC (version 0.11.5)