##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140135_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5885589
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.743098269349083	33.0	28.0	33.0	18.0	34.0
2	31.102091906179652	33.0	30.0	33.0	27.0	34.0
3	32.34789874046591	33.0	33.0	33.0	30.0	34.0
4	32.424884748153495	33.0	33.0	34.0	31.0	34.0
5	32.89711564976759	33.0	33.0	34.0	32.0	34.0
6	36.70217492250988	38.0	37.0	38.0	34.0	38.0
7	36.90579855304202	38.0	38.0	38.0	35.0	38.0
8	37.12385370436162	38.0	38.0	38.0	36.0	38.0
9	37.17101398007914	38.0	38.0	38.0	36.0	38.0
10-11	37.25104055006219	38.0	38.0	38.0	36.0	38.0
12-13	37.29794978888265	38.0	38.0	38.0	36.5	38.0
14-15	37.301560132724184	38.0	38.0	38.0	36.5	38.0
16-17	37.34456288062248	38.0	38.0	38.0	37.0	38.0
18-19	37.34797638774981	38.0	38.0	38.0	37.0	38.0
20-21	37.371022165168895	38.0	38.0	38.0	37.0	38.0
22-23	37.30401064707614	38.0	38.0	38.0	37.0	38.0
24-25	37.374247919648084	38.0	38.0	38.0	37.0	38.0
26-27	37.33677167525521	38.0	38.0	38.0	37.0	38.0
28-29	37.29174611405368	38.0	38.0	38.0	37.0	38.0
30-31	37.30753850188054	38.0	38.0	38.0	37.0	38.0
32-33	37.28592426670594	38.0	38.0	38.0	37.0	38.0
34-35	37.185379447145735	38.0	38.0	38.0	36.5	38.0
36-37	37.207551936212354	38.0	38.0	38.0	37.0	38.0
38-39	37.15948944309398	38.0	38.0	38.0	36.0	38.0
40-41	37.263987471647624	38.0	38.0	38.0	37.0	38.0
42-43	37.25783591091508	38.0	38.0	38.0	37.0	38.0
44-45	37.22518943088327	38.0	38.0	38.0	37.0	38.0
46-47	37.20099716777619	38.0	38.0	38.0	37.0	38.0
48-49	37.147912223769794	38.0	38.0	38.0	36.5	38.0
50-51	37.109843623079186	38.0	38.0	38.0	36.0	38.0
52-53	37.01417903030803	38.0	38.0	38.0	36.0	38.0
54-55	37.05311990162329	38.0	38.0	38.0	36.0	38.0
56-57	37.01867530417074	38.0	38.0	38.0	36.0	38.0
58-59	36.98987176674221	38.0	38.0	38.0	36.0	38.0
60-61	36.97488887501885	38.0	38.0	38.0	36.0	38.0
62-63	36.985855035244725	38.0	38.0	38.0	36.0	38.0
64-65	36.99287300151274	38.0	38.0	38.0	36.0	38.0
66-67	36.98143549557511	38.0	38.0	38.0	36.0	38.0
68-69	36.96356026615487	38.0	38.0	38.0	36.0	38.0
70-71	36.965183136512934	38.0	38.0	38.0	36.0	38.0
72-73	36.98011778332043	38.0	38.0	38.0	36.0	38.0
74-75	36.938201420425706	38.0	38.0	38.0	36.0	38.0
76-77	36.95519945069165	38.0	38.0	38.0	36.0	38.0
78-79	36.96991867738739	38.0	38.0	38.0	36.0	38.0
80-81	36.962469667878295	38.0	38.0	38.0	36.0	38.0
82-83	36.92729981330139	38.0	38.0	38.0	36.0	38.0
84-85	36.92777159857349	38.0	38.0	38.0	36.0	38.0
86-87	36.930229121739515	38.0	38.0	38.0	36.0	38.0
88-89	36.92024904877919	38.0	38.0	38.0	35.5	38.0
90-91	36.93317281706107	38.0	38.0	38.0	36.0	38.0
92-93	36.915175242423715	38.0	38.0	38.0	35.0	38.0
94-95	36.85597385496372	38.0	38.0	38.0	35.0	38.0
96-97	36.89472800969045	38.0	38.0	38.0	35.0	38.0
98-99	36.919661661804476	38.0	38.0	38.0	35.0	38.0
100	35.48750051896613	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	18.0
19	28.0
20	62.0
21	155.0
22	457.0
23	1248.0
24	3089.0
25	6115.0
26	10936.0
27	17850.0
28	27387.0
29	39202.0
30	52479.0
31	67726.0
32	86850.0
33	115016.0
34	169186.0
35	297093.0
36	830191.0
37	4160498.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.35764118085717	21.62211802421134	14.388296566409922	24.63194422852156
2	14.330820740345981	20.337524462604417	42.97351411132856	22.358140685721047
3	17.676735497500758	26.76321775101863	31.12371591016634	24.43633084131427
4	12.458175383976013	18.936490468498565	37.4910480497364	31.114286097789023
5	13.27314904251724	37.51955836535647	35.864023124958265	13.343269467168026
6	29.314160400938633	37.97106797637416	18.46669891492593	14.248072707761281
7	25.876764415592053	33.121612807146406	21.94140637411141	19.060216403150136
8	24.11050109003534	37.2977623819808	21.267370181641972	17.324366346341886
9	24.683137065805987	17.506489155121095	20.405434358396416	37.404939420676506
10-11	23.849669081548168	26.915785319022444	28.636156891009552	20.598388708419836
12-13	24.991763780991164	24.01878554550785	28.49012222905813	22.49932844444286
14-15	22.237961230388326	25.873782556002467	25.493693154584868	26.39456305902434
16-17	22.197897270774426	29.56065229835111	26.10338574440043	22.138064686474028
18-19	22.22414782955453	27.881712433538937	27.796317411902187	22.097822325004344
20-21	22.75078924705733	26.8779220576213	27.971199459282616	22.400089236038752
22-23	22.22548175032411	27.76129889504546	27.80503490895127	22.20818444567916
24-25	22.470365401269845	27.941490460351275	28.00952065940439	21.57862347897449
26-27	22.405370869850945	28.057346538503307	28.185578443920146	21.351704147725602
28-29	21.38489973531766	27.245679222551207	28.757531613843113	22.61188942828802
30-31	22.495263485026623	26.836629207975726	29.188250175130214	21.479857131867437
32-33	21.7576527836475	27.962246278708918	28.5548353845246	21.72526555311898
34-35	21.615893566350323	28.013135763901687	29.378647107294075	20.99232356245392
36-37	22.498933979530175	27.436589702864662	28.388174474177408	21.676301843427755
38-39	21.95217276042427	26.94049357407281	28.909470546430487	22.197863119072437
40-41	21.980983791568466	26.823606041260494	29.214782637332597	21.98062752983844
42-43	22.468635507416483	26.619602988189488	29.25971535348687	21.652046150907154
44-45	21.735856380842243	27.270957193044627	29.075311254422008	21.917875171691126
46-47	22.68867239719	26.541232108393643	28.143765958098598	22.62632953631776
48-49	22.297087909461542	26.606556898668245	28.52798919255875	22.568365999311464
50-51	21.81256519514215	27.12280253026146	28.904633226988853	22.15999904760754
52-53	21.974319092653353	27.4770994879713	29.25372781871232	21.29485360066303
54-55	21.80093122253299	27.709298478964172	28.36295051701609	22.126819781486745
56-57	22.47760632042222	28.496286866271497	27.404944182216273	21.621162631090012
58-59	21.383805614191342	29.474881079495564	28.37295180843305	20.768361497880047
60-61	22.138612913400017	30.324871962392237	26.814435583190654	20.72207954101709
62-63	21.405594895644786	30.893155812011535	26.84548508143674	20.855764210906937
64-65	21.44281191016112	30.6488587727703	26.950426172093266	20.95790314497532
66-67	21.713056629425825	30.380258765802022	26.878444547446012	21.028240057326148
68-69	21.855350500501604	29.664861232941313	27.244585504224688	21.235202762332396
70-71	22.054026490223606	28.733123896484038	27.59428837154223	21.618561241750125
72-73	22.33717081749327	28.27691375883341	27.696991963778334	21.688923459894983
74-75	22.162633235858884	28.234305356687056	27.766043113690426	21.837018293763634
76-77	22.238903528545347	27.991009221469298	27.800044467238514	21.970042782746837
78-79	22.243418124088514	28.026918385255446	27.770254515688524	21.95940897496752
80-81	22.306310475048562	27.98547384486772	27.74852691101047	21.95968876907325
82-83	22.270704012340133	27.87371724640889	27.89993163382461	21.955647107426365
84-85	22.420692284216436	27.829734270686146	27.817570260733383	21.932003184364035
86-87	22.222143042501713	27.86810173941157	27.88456868497845	22.02518653310826
88-89	22.324708517880453	27.837233067108624	27.839669028183295	21.99838938682763
90-91	22.38788050627835	27.832531193707517	27.871693614701528	21.907894685312606
92-93	22.377907949185126	27.82308093080611	27.811455297460714	21.987555822548053
94-95	22.20813088065486	27.95037098857881	27.79200631080726	22.049491819959073
96-97	22.296867354064396	28.04817240552635	27.89160686329655	21.76335337711271
98-99	23.389219617910445	29.929383582535067	28.116567214042483	18.564829585512005
100	22.348175632418073	30.830025833274494	22.066281164081357	24.755517370226073
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	971.0
1	860.0
2	931.0
3	1240.0
4	1683.5
5	2408.0
6	3021.5
7	3713.5
8	4550.5
9	5117.0
10	5280.5
11	5561.0
12	6528.0
13	8298.0
14	11601.5
15	15438.5
16	18412.0
17	20221.0
18	20277.5
19	18944.5
20	17328.0
21	16164.0
22	16643.5
23	19321.5
24	24135.5
25	31537.0
26	42139.5
27	54303.5
28	65505.0
29	77584.5
30	89996.5
31	102416.5
32	117148.0
33	132542.5
34	147589.5
35	162276.0
36	178425.0
37	192176.0
38	201511.0
39	211262.5
40	222696.0
41	233195.0
42	244518.0
43	273453.0
44	314855.5
45	333861.5
46	322270.0
47	298173.0
48	268185.5
49	246259.0
50	237268.0
51	226899.0
52	214209.0
53	200414.5
54	185907.5
55	170379.5
56	153638.5
57	136637.5
58	119858.0
59	103744.0
60	86409.5
61	69215.5
62	53944.0
63	41248.5
64	31116.0
65	22812.5
66	16546.5
67	12469.5
68	9446.5
69	6772.0
70	4908.0
71	3510.0
72	2431.5
73	1632.5
74	1071.5
75	684.5
76	433.0
77	267.5
78	186.0
79	134.5
80	94.5
81	60.5
82	38.0
83	23.5
84	18.5
85	18.0
86	13.0
87	8.5
88	4.5
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0186387462665164
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.37221483234108E-4
26-27	5.183291820264173E-4
28-29	0.0040291092090051096
30-31	0.006475484165824471
32-33	0.00480072022741259
34-35	0.0021289970423186474
36-37	0.0
38-39	8.635257185160034E-6
40-41	4.692205646860953E-4
42-43	0.0
44-45	0.0
46-47	3.7293695935795173E-4
48-49	0.001584354951364331
50-51	0.003147485866751321
52-53	0.0
54-55	0.003018073044501304
56-57	0.04082650541517976
58-59	0.06441471892586761
60-61	0.04431478301941585
62-63	0.01459254010741135
64-65	3.4562158890692946E-4
66-67	0.0
68-69	0.0
70-71	0.002859720057573012
72-73	0.005359585120971883
74-75	0.004624675031224316
76-77	0.003268621587814913
78-79	0.008140700836309382
80-81	0.017200300211803327
82-83	0.04468222857401626
84-85	0.02057683547815625
86-87	0.002159732500137039
88-89	0.001372977391159155
90-91	1.4546020924786776E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	42.0
20-21	158.0
22-23	386.0
24-25	487.0
26-27	1504.0
28-29	4138.0
30-31	11904.0
32-33	14754.0
34-35	20451.0
36-37	32234.0
38-39	36694.0
40-41	34962.0
42-43	38917.0
44-45	46162.0
46-47	45880.0
48-49	42833.0
50-51	39780.0
52-53	37432.0
54-55	40637.0
56-57	43629.0
58-59	46760.0
60-61	64173.0
62-63	58901.0
64-65	69042.0
66-67	79749.0
68-69	77754.0
70-71	104231.0
72-73	48395.0
74-75	44269.0
76-77	46072.0
78-79	46696.0
80-81	47057.0
82-83	44191.0
84-85	43366.0
86-87	44777.0
88-89	46551.0
90-91	47823.0
92-93	46748.0
94-95	54691.0
96-97	171433.0
98-99	378369.0
100-101	3781557.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.43556686761285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.44202620554711	80.86727423010667
2	6.392514068528038	11.306512107189343
3	1.3098840452864309	3.475210142272421
4	0.4369254153525495	1.545589871422796
5	0.17860281773593145	0.789742071531502
6	0.0856242772309605	0.4543338896729777
7	0.049961095505346574	0.30928364616395876
8	0.027755803334618197	0.19636801614103513
9	0.01714192650497908	0.13643603889037043
>10	0.05805828545488156	0.7557032777374647
>50	0.0010120306598338743	0.059773540035203146
>100	4.4089226209559655E-4	0.06911682636937916
>500	5.31365972092098E-5	0.03465634246673232
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6990652932102464E-5
2	0.0	1.6990652932102464E-5	0.0	0.0	1.6990652932102464E-5
3	0.0	1.6990652932102464E-5	0.0	0.0	1.6990652932102464E-5
4	0.0	3.398130586420493E-5	0.0	0.0	1.6990652932102464E-5
5	0.0	3.398130586420493E-5	0.0	0.0	1.6990652932102464E-5
6	3.398130586420493E-5	3.398130586420493E-5	0.0	1.6990652932102464E-5	1.6990652932102464E-5
7	3.398130586420493E-5	3.398130586420493E-5	0.0	1.6990652932102464E-5	3.398130586420493E-5
8	3.398130586420493E-5	3.398130586420493E-5	0.0	1.6990652932102464E-5	3.398130586420493E-5
9	3.398130586420493E-5	6.796261172840985E-5	0.0	5.097195879630739E-5	5.097195879630739E-5
10-11	4.2476632330256154E-5	6.796261172840985E-5	0.0	9.344859112656354E-5	5.097195879630739E-5
12-13	5.097195879630739E-5	6.796261172840985E-5	0.0	1.0194391759261477E-4	5.097195879630739E-5
14-15	5.097195879630739E-5	9.344859112656354E-5	0.0	1.0194391759261477E-4	1.0194391759261477E-4
16-17	1.0194391759261477E-4	1.0194391759261477E-4	0.0	1.0194391759261477E-4	1.0194391759261477E-4
18-19	1.0194391759261477E-4	1.1893457052471724E-4	0.0	1.0194391759261477E-4	1.0194391759261477E-4
20-21	1.0194391759261477E-4	1.4442054992287094E-4	0.0	1.4442054992287094E-4	1.0194391759261477E-4
22-23	1.0194391759261477E-4	1.5291587638892215E-4	0.0	1.8689718225312708E-4	1.5291587638892215E-4
24-25	1.0194391759261477E-4	1.8689718225312708E-4	0.0	2.3786914104943448E-4	2.0388783518522955E-4
26-27	1.0194391759261477E-4	2.2087848811733202E-4	0.0	2.718504469136394E-4	2.0388783518522955E-4
28-29	1.0194391759261477E-4	2.2087848811733202E-4	0.0	6.116635055556886E-4	2.0388783518522955E-4
30-31	1.0194391759261477E-4	2.4636446751548574E-4	0.0	0.0016565886608799901	2.0388783518522955E-4
32-33	1.0194391759261477E-4	2.5485979398153695E-4	0.0	0.003635999727469927	2.2087848811733202E-4
34-35	1.0194391759261477E-4	3.058317527778443E-4	0.0	0.006379990176004475	2.2087848811733202E-4
36-37	1.0194391759261477E-4	3.6529903804020296E-4	0.0	0.010857027223613473	2.2087848811733202E-4
38-39	1.0194391759261477E-4	3.7379436450625417E-4	0.0	0.017143568808491383	2.2087848811733202E-4
40-41	1.0194391759261477E-4	3.7379436450625417E-4	0.0	0.023540549637427962	2.2087848811733202E-4
42-43	1.0194391759261477E-4	3.7379436450625417E-4	0.0	0.029895053834034284	2.2087848811733202E-4
44-45	1.1043924405866601E-4	3.992803439044079E-4	0.0	0.03612212813364984	2.3786914104943448E-4
46-47	1.1893457052471724E-4	4.2476632330256157E-4	0.0	0.04310528648874395	2.4636446751548574E-4
48-49	1.1893457052471724E-4	4.2476632330256157E-4	0.0	0.050470734534810366	2.718504469136394E-4
50-51	1.1893457052471724E-4	4.587476291667665E-4	0.0	0.057165051790058735	2.718504469136394E-4
52-53	1.1893457052471724E-4	4.587476291667665E-4	0.0	0.06350256533373295	2.888410998457419E-4
54-55	1.1893457052471724E-4	4.587476291667665E-4	0.0	0.07027334052717579	3.143270792438955E-4
56-57	1.1893457052471724E-4	4.587476291667665E-4	0.0	0.0765938634179179	3.2282240570994676E-4
58-59	1.1893457052471724E-4	4.587476291667665E-4	0.0	0.08245563867949325	3.31317732175998E-4
60-61	1.1893457052471724E-4	4.927289350309714E-4	0.0	0.08877616157023537	3.3981305864204923E-4
62-63	1.359252234568197E-4	4.927289350309714E-4	0.0	0.09406025463211923	3.568037115741517E-4
64-65	1.359252234568197E-4	5.437008938272788E-4	0.0	0.09901302996182709	3.568037115741517E-4
66-67	1.5291587638892215E-4	5.5219622029333E-4	0.0	0.10435659030897332	3.6529903804020296E-4
68-69	1.5291587638892215E-4	5.606915467593812E-4	0.0	0.10991253381777083	3.7379436450625417E-4
70-71	1.5291587638892215E-4	5.606915467593812E-4	0.0	0.11474637457695397	3.7379436450625417E-4
72-73	2.2087848811733202E-4	5.606915467593812E-4	0.0	0.11792362667525713	3.7379436450625417E-4
74-75	2.2087848811733202E-4	5.606915467593812E-4	0.0	0.12104990681476399	3.7379436450625417E-4
76-77	2.2937381458338325E-4	5.776821996914838E-4	0.0	0.12392982248675535	3.7379436450625417E-4
78-79	2.3786914104943448E-4	5.86177526157535E-4	0.0	0.12568835506522796	3.7379436450625417E-4
80-81	2.3786914104943448E-4	5.946728526235862E-4	0.0	0.12633399987664784	3.8228969097230537E-4
82-83	2.3786914104943448E-4	5.946728526235862E-4	0.0	0.12675027087348437	4.2476632330256157E-4
84-85	2.3786914104943448E-4	5.946728526235862E-4	0.0	0.12697964468806777	4.2476632330256157E-4
86-87	2.3786914104943448E-4	5.946728526235862E-4	0.0	0.12713256056445668	4.2476632330256157E-4
88	2.3786914104943448E-4	5.946728526235862E-4	0.0	0.12714105589092273	4.2476632330256157E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	10810	0.0	61.697758	1
GGTATCA	7535	0.0	48.61243	1
TATACAC	14555	0.0	47.64856	3
TTATACA	14235	0.0	47.41823	2
GTATCAA	13335	0.0	35.97919	1
TACACAT	20835	0.0	33.428776	5
ATACACA	23595	0.0	30.2542	4
ACACATC	23000	0.0	29.711447	6
ATCAACG	15860	0.0	29.685865	3
TCAACGC	15860	0.0	29.685865	4
CAACGCA	16195	0.0	29.045656	5
TATCAAC	16470	0.0	28.899294	2
ACATCTC	24015	0.0	28.402792	8
AACGCAG	16855	0.0	27.93342	6
CACATCT	24985	0.0	27.232317	7
CATCTCC	27330	0.0	24.771763	9
ACGCAGA	19235	0.0	24.433117	7
CGCAGAG	19680	0.0	23.902153	8
GTGGTAT	3730	0.0	22.932798	1
TGGTATC	3540	0.0	22.488945	2
>>END_MODULE