FastQCFastQC Report
Fri 27 May 2016
SRR2140134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6487510
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC92550.055.2025221
GGTATCA94450.050.9739531
TATACAC126850.042.1866073
TTATACA130000.040.0550882
GTATCAA176100.038.415911
ATCAACG209450.031.9879823
TCAACGC210500.031.8689674
TATCAAC217100.031.1019652
CAACGCA217050.030.946575
AACGCAG224600.029.9062886
TACACAT190200.028.4271725
ACACATC197850.026.314276
GTGGTAT42900.026.166141
ATACACA216800.025.8644714
ACGCAGA260800.025.7551867
CGCAGAG263450.025.4961198
ACATCTC206800.025.1960648
TGGTATC42650.024.7183282
CACATCT217600.023.7297957
CATCTCC227250.022.7409089
Produced by FastQC (version 0.11.5)