##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140134_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6487510
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.260691390071074	32.0	27.0	33.0	18.0	34.0
2	31.068452611248382	33.0	30.0	33.0	27.0	34.0
3	32.32028929435176	33.0	33.0	33.0	30.0	34.0
4	32.44586397554686	33.0	33.0	34.0	31.0	34.0
5	32.92678346545901	33.0	33.0	34.0	32.0	34.0
6	36.74996554918605	38.0	37.0	38.0	34.0	38.0
7	36.977078108550124	38.0	38.0	38.0	35.0	38.0
8	37.1576879264926	38.0	38.0	38.0	36.0	38.0
9	37.20345710449772	38.0	38.0	38.0	36.0	38.0
10-11	37.27627101923542	38.0	38.0	38.0	36.5	38.0
12-13	37.32078594098506	38.0	38.0	38.0	37.0	38.0
14-15	37.318423170060626	38.0	38.0	38.0	37.0	38.0
16-17	37.360882372435654	38.0	38.0	38.0	37.0	38.0
18-19	37.36417570069256	38.0	38.0	38.0	37.0	38.0
20-21	37.382705264547994	38.0	38.0	38.0	37.0	38.0
22-23	37.31801062328941	38.0	38.0	38.0	37.0	38.0
24-25	37.38591985448327	38.0	38.0	38.0	37.0	38.0
26-27	37.35333805945032	38.0	38.0	38.0	37.0	38.0
28-29	37.3187785044767	38.0	38.0	38.0	37.0	38.0
30-31	37.32457240894091	38.0	38.0	38.0	37.0	38.0
32-33	37.298208039928774	38.0	38.0	38.0	37.0	38.0
34-35	37.20466367208378	38.0	38.0	38.0	37.0	38.0
36-37	37.22105242427793	38.0	38.0	38.0	37.0	38.0
38-39	37.193256670639045	38.0	38.0	38.0	37.0	38.0
40-41	37.264493628787676	38.0	38.0	38.0	37.0	38.0
42-43	37.247522575349905	38.0	38.0	38.0	37.0	38.0
44-45	37.208258859819225	38.0	38.0	38.0	37.0	38.0
46-47	37.168370018109265	38.0	38.0	38.0	37.0	38.0
48-49	37.10851128205424	38.0	38.0	38.0	36.0	38.0
50-51	37.07124255584091	38.0	38.0	38.0	36.0	38.0
52-53	36.98209042081287	38.0	38.0	38.0	36.0	38.0
54-55	37.02056504504306	38.0	38.0	38.0	36.0	38.0
56-57	36.98536076174159	38.0	38.0	38.0	36.0	38.0
58-59	36.96361968966916	38.0	38.0	38.0	36.0	38.0
60-61	36.9559628378279	38.0	38.0	38.0	36.0	38.0
62-63	36.96876793266112	38.0	38.0	38.0	36.0	38.0
64-65	36.980843021183986	38.0	38.0	38.0	36.0	38.0
66-67	36.9758561524135	38.0	38.0	38.0	36.0	38.0
68-69	36.9626553864981	38.0	38.0	38.0	36.0	38.0
70-71	36.9646578704975	38.0	38.0	38.0	36.0	38.0
72-73	36.98129386661323	38.0	38.0	38.0	36.0	38.0
74-75	36.934321017368646	38.0	38.0	38.0	36.0	38.0
76-77	36.95034886755626	38.0	38.0	38.0	36.0	38.0
78-79	36.96283916498001	38.0	38.0	38.0	36.0	38.0
80-81	36.952379831353355	38.0	38.0	38.0	36.0	38.0
82-83	36.90884229200218	38.0	38.0	38.0	35.5	38.0
84-85	36.90734134533231	38.0	38.0	38.0	35.0	38.0
86-87	36.907062603249614	38.0	38.0	38.0	35.0	38.0
88-89	36.89263295078097	38.0	38.0	38.0	35.0	38.0
90-91	36.903270857572736	38.0	38.0	38.0	35.0	38.0
92-93	36.882628804867664	38.0	38.0	38.0	35.0	38.0
94-95	36.82311083756236	38.0	38.0	38.0	35.0	38.0
96-97	36.85401051715231	38.0	38.0	38.0	35.0	38.0
98-99	36.88320278142352	38.0	38.0	38.0	35.0	38.0
100	35.41392148840465	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	9.0
19	20.0
20	40.0
21	132.0
22	456.0
23	1302.0
24	3231.0
25	6546.0
26	11788.0
27	19355.0
28	30209.0
29	43792.0
30	58990.0
31	75822.0
32	97319.0
33	128716.0
34	187716.0
35	327573.0
36	905822.0
37	4588671.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.094196386595165	21.76243273613451	14.185010890156624	23.9583599871137
2	14.680497065547113	20.390177090855495	42.83104865142203	22.098277192175367
3	17.985821987172272	26.932937290270072	30.595652261036975	24.485588461520678
4	12.600242620049912	18.666221709099485	37.91853885389001	30.814996816960587
5	13.27968665944253	37.70896692259434	35.64304332478871	13.368303093174422
6	29.291715927990865	37.94908986652815	18.612688072927828	14.146506132553169
7	26.110711197362317	33.08038060827652	22.220975381926195	18.58793281243497
8	24.011508267424635	37.21933376595951	21.202125314643062	17.56703265197279
9	25.048531717099472	17.362239133350084	20.728214677125738	36.861014472424706
10-11	24.032178755793826	27.01108745882473	28.56767080127815	20.3890629841033
12-13	25.219514112502335	24.225049364085756	28.607416404753135	21.948020118658775
14-15	22.29912555048085	25.76665777779148	25.99679229781534	25.937424373912332
16-17	21.872698462121832	29.397973953026664	26.565562134008267	22.163765450843236
18-19	21.94859815244986	27.87893197852489	28.63797512450848	21.53449474451677
20-21	22.614737908784015	26.989731598500576	28.56259720245	21.83293329026541
22-23	22.296121747077873	27.512235080647056	28.427269664591886	21.764373507683185
24-25	22.255983420939017	27.70732247255612	28.7233033128446	21.313390793660265
26-27	22.217491536608144	27.841577792861493	28.809667249843802	21.13126342068656
28-29	21.81613076815231	27.35318836133708	29.13918770707063	21.691493163439983
30-31	22.220277813393825	27.911371186727603	28.533370159595993	21.33498084028258
32-33	22.752699815339398	27.236939854785224	28.773362466524222	21.236997863351153
34-35	22.140063334736652	27.244002202610208	29.038706120819885	21.577228341833255
36-37	21.755837194491367	27.77197365149805	28.912285978356966	21.55990317565362
38-39	21.725605101683634	27.08301632536258	29.28988492532388	21.90149364762991
40-41	21.96091774684926	26.922459385420588	29.40197029462761	21.71465257310254
42-43	22.306626754308244	26.78118563595528	29.247703163333505	21.664484446402973
44-45	21.814828894456625	27.30318644159746	29.096426945916935	21.78555771802898
46-47	22.477168902940157	26.7880635659764	28.46217375226831	22.272593778815132
48-49	22.18522769489106	26.865214716169948	28.557249386969524	22.392308201969467
50-51	21.80898054190863	27.117190094205192	28.931017510190525	22.14281185369565
52-53	22.0405922928742	27.239805692977264	29.20331915935427	21.516282854794262
54-55	21.955645628832578	27.34535518944652	28.593454429805238	22.10554475191567
56-57	22.390430924872714	27.98048733422772	27.85565995673608	21.77342178416348
58-59	21.666752333380416	28.674569932766914	28.513421169896606	21.145256563956064
60-61	22.084260443776724	29.355138139986025	27.470866452597107	21.08973496364014
62-63	21.534560685643857	29.871795825661206	27.444448748903326	21.149194739791614
64-65	21.620627632466032	29.676337279149916	27.483413613684338	21.219621474699714
66-67	21.740902678956115	29.526441205233734	27.423099896877567	21.309556218932588
68-69	21.888086552761287	29.018497971153977	27.700162072114253	21.39325340397048
70-71	21.998331364002528	28.265854356280478	27.997386049264755	21.73842823045224
72-73	22.248255931557136	27.91365336365409	28.033113418439843	21.804977286348937
74-75	22.017118721191274	28.014619102836996	28.044036668189566	21.924225507782165
76-77	22.240232720571846	27.771841702456495	28.020946337548857	21.966979239422805
78-79	22.208196498945743	27.837666241301662	27.98564564192416	21.968491617828434
80-81	22.211947064110202	27.807083012497568	27.96812639643422	22.01284352695801
82-83	22.236043819241804	27.719671630971387	28.05281417656163	21.991470373225177
84-85	22.35774999074508	27.721138539944224	27.980305150053063	21.940806319257632
86-87	22.20278812372029	27.742031355062046	28.025500130608812	22.029680390608846
88-89	22.259873317051856	27.726387529344798	27.977206847252518	22.036532306350825
90-91	22.300083895096034	27.708779519682235	28.0211008757728	21.970035709448936
92-93	22.322676267206113	27.733775305316183	27.912153061173395	22.03139536630431
94-95	22.224054913826784	27.87251725240221	27.90654797436251	21.996879859408494
96-97	22.326788059904455	27.923570471836296	27.99875352253648	21.750887945722766
98-99	23.310272063871867	29.845911561093708	28.230285385287594	18.61353098974683
100	22.320221555845468	30.743306387415352	22.195071229249898	24.741400827489283
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1219.0
1	1115.5
2	1368.0
3	1880.5
4	2450.5
5	3463.0
6	4416.5
7	5735.0
8	7302.0
9	8296.0
10	8461.0
11	8530.0
12	9533.5
13	11837.0
14	16489.5
15	22092.0
16	26098.5
17	28147.5
18	28132.5
19	25943.5
20	22902.5
21	20528.5
22	20316.0
23	22753.0
24	28299.0
25	37320.0
26	49136.0
27	62293.0
28	74702.5
29	87545.0
30	100214.0
31	113800.0
32	130200.0
33	146657.5
34	163202.5
35	179436.5
36	195792.5
37	210996.5
38	221039.5
39	229023.5
40	240153.0
41	250330.5
42	259729.0
43	285353.0
44	317314.5
45	329733.5
46	316364.5
47	292881.5
48	275264.0
49	263846.5
50	257246.5
51	248652.0
52	236317.5
53	223305.5
54	209712.0
55	193752.0
56	176471.5
57	158210.5
58	139227.0
59	120481.5
60	100785.5
61	81001.5
62	62958.5
63	48186.0
64	36426.0
65	26697.5
66	19144.0
67	14085.5
68	10499.5
69	7524.0
70	5386.5
71	3794.0
72	2639.5
73	1810.5
74	1190.5
75	753.5
76	480.0
77	316.0
78	223.0
79	142.0
80	88.5
81	60.0
82	40.5
83	30.5
84	22.5
85	15.0
86	11.0
87	8.0
88	4.5
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.017294771029254676
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.8540032649574064E-4
26-27	2.852279225493641E-4
28-29	0.0036629559252322062
30-31	0.00598982701981637
32-33	0.0041687084830665366
34-35	0.00218674304861913
36-37	0.0
38-39	0.0
40-41	3.779715949621741E-4
42-43	0.0
44-45	0.0
46-47	3.298030995056171E-4
48-49	0.0012732024935305905
50-51	0.002581882440682885
52-53	0.0
54-55	0.0030063398987766185
56-57	0.04129716135827115
58-59	0.06422138361057511
60-61	0.04358179277798331
62-63	0.014031707040530856
64-65	1.898863262770136E-4
66-67	0.0
68-69	2.6567740210009133E-5
70-71	0.0030467002528671333
72-73	0.00533723380204282
74-75	0.004354899045061789
76-77	0.0031206765626787887
78-79	0.008061068404633014
80-81	0.01625881920278221
82-83	0.04428404733835677
84-85	0.019865203844847072
86-87	0.0018866441929091741
88-89	0.0014234718931057035
90-91	1.2888617853372937E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	47.0
20-21	188.0
22-23	427.0
24-25	555.0
26-27	1617.0
28-29	4155.0
30-31	12218.0
32-33	15593.0
34-35	21354.0
36-37	33549.0
38-39	39067.0
40-41	36942.0
42-43	42290.0
44-45	50537.0
46-47	51405.0
48-49	47141.0
50-51	42870.0
52-53	39750.0
54-55	43230.0
56-57	45606.0
58-59	46503.0
60-61	52040.0
62-63	53137.0
64-65	65801.0
66-67	76169.0
68-69	75480.0
70-71	95137.0
72-73	52625.0
74-75	46612.0
76-77	48634.0
78-79	50600.0
80-81	51066.0
82-83	46473.0
84-85	45652.0
86-87	47749.0
88-89	49321.0
90-91	50674.0
92-93	50619.0
94-95	59168.0
96-97	192518.0
98-99	428132.0
100-101	4274859.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.16116670222176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.96164240880462	77.51200068390676
2	7.255068465748982	12.502103270268606
3	1.6347184139696016	4.225477373316791
4	0.5671647156351237	1.9547029444582433
5	0.23966538424022404	1.0324924562136986
6	0.11822189554070413	0.6111681869721154
7	0.07516422004119448	0.4533365825108285
8	0.0413867802693041	0.28527466192413864
9	0.026821457703495487	0.2079871279548718
>10	0.07810865427216018	0.994140819782043
>50	0.0012839971771625525	0.07257234530581787
>100	6.995368550890658E-4	0.11359356479330152
>500	5.40697423771268E-5	0.03514998259280665
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.082846885785147E-5	0.0	0.0	0.0
3	0.0	3.082846885785147E-5	0.0	0.0	0.0
4	0.0	6.165693771570295E-5	0.0	0.0	1.5414234428925736E-5
5	0.0	6.165693771570295E-5	0.0	0.0	1.5414234428925736E-5
6	1.5414234428925736E-4	6.165693771570295E-5	0.0	0.0	1.5414234428925736E-5
7	1.5414234428925736E-4	7.707117214462868E-5	0.0	3.082846885785147E-5	1.5414234428925736E-5
8	1.5414234428925736E-4	7.707117214462868E-5	0.0	3.082846885785147E-5	1.5414234428925736E-5
9	1.5414234428925736E-4	1.0789964100248014E-4	0.0	3.082846885785147E-5	1.5414234428925736E-5
10-11	1.5414234428925736E-4	1.0789964100248014E-4	0.0	3.082846885785147E-5	1.5414234428925736E-5
12-13	1.5414234428925736E-4	1.0789964100248014E-4	0.0	3.082846885785147E-5	1.5414234428925736E-5
14-15	1.5414234428925736E-4	1.5414234428925736E-4	0.0	4.624270328677721E-5	3.082846885785147E-5
16-17	2.157992820049603E-4	1.5414234428925736E-4	0.0	6.165693771570295E-5	4.6242703286777206E-5
18-19	2.157992820049603E-4	1.695565787181831E-4	0.0	6.936405493016581E-5	4.6242703286777206E-5
20-21	2.157992820049603E-4	1.8497081314710882E-4	0.0	7.707117214462868E-5	4.6242703286777206E-5
22-23	2.157992820049603E-4	1.9267793036157168E-4	0.0	1.1560675821694301E-4	6.165693771570295E-5
24-25	2.157992820049603E-4	2.2350639921942317E-4	0.0	1.464352270747945E-4	7.707117214462868E-5
26-27	2.157992820049603E-4	2.466277508628118E-4	0.0	2.0809216479049744E-4	7.707117214462868E-5
28-29	2.157992820049603E-4	2.466277508628118E-4	0.0	5.549124394413265E-4	7.707117214462868E-5
30-31	2.157992820049603E-4	2.620419852917375E-4	0.0	0.0016724444355384423	7.707117214462868E-5
32-33	2.157992820049603E-4	2.620419852917375E-4	0.0	0.0036531735596553993	7.707117214462868E-5
34-35	2.3121351643388605E-4	3.082846885785147E-4	0.0	0.006520221163435586	7.707117214462868E-5
36-37	2.3121351643388605E-4	3.699416262942177E-4	0.0	0.010697478693674461	7.707117214462868E-5
38-39	2.3121351643388605E-4	4.007700951520691E-4	0.0	0.01666278741766872	7.707117214462868E-5
40-41	2.3121351643388605E-4	4.855483845111607E-4	0.0	0.022859309658096866	7.707117214462868E-5
42-43	2.3121351643388605E-4	5.086697361545492E-4	0.0	0.02886315396816344	8.477828935909155E-5
44-45	2.3121351643388605E-4	5.626195566557893E-4	0.0	0.035329425311097784	9.248540657355441E-5
46-47	2.3121351643388605E-4	6.011551427281037E-4	0.0	0.04208856710818172	1.0019252378801728E-4
48-49	2.3121351643388605E-4	6.165693771570294E-4	0.0	0.049880462612003684	1.233138754314059E-4
50-51	2.7745621972066325E-4	6.936405493016581E-4	0.0	0.05681686810502026	1.233138754314059E-4
52-53	2.9287045414958895E-4	6.936405493016581E-4	0.0	0.06354518143324635	1.3102099264586874E-4
54-55	2.9287045414958895E-4	7.321761353739724E-4	0.0	0.06945654033673937	1.464352270747945E-4
56-57	2.9287045414958895E-4	7.707117214462868E-4	0.0	0.07436597400235222	1.618494615037202E-4
58-59	2.9287045414958895E-4	8.015401903041382E-4	0.0	0.07933706460568077	1.695565787181831E-4
60-61	3.8535586072314335E-4	8.323686591619897E-4	0.0	0.0842464982712936	1.695565787181831E-4
62-63	4.315985640099206E-4	8.323686591619897E-4	0.0	0.08849311985646265	1.8497081314710882E-4
64-65	4.315985640099206E-4	0.0010096323550946359	0.0	0.09291700513756435	1.9267793036157168E-4
66-67	4.778412672966978E-4	0.0010327537067380242	0.0	0.09722528366044908	2.0038504757603456E-4
68-69	4.778412672966978E-4	0.0010327537067380242	0.0	0.1013717127218301	2.0038504757603456E-4
70-71	5.009626189400864E-4	0.001040460823952487	0.0	0.10559521295535576	2.0038504757603456E-4
72-73	5.857409082991779E-4	0.00104816794116695	0.0	0.10883220218543016	2.157992820049603E-4
74-75	5.857409082991779E-4	0.0010712892928103385	0.0	0.11166071420313803	2.157992820049603E-4
76-77	5.934480255136408E-4	0.0011252391133115788	0.0	0.11437361946262896	2.157992820049603E-4
78-79	6.011551427281037E-4	0.0011406533477405045	0.0	0.11612313507031202	2.157992820049603E-4
80-81	6.011551427281037E-4	0.0011406533477405045	0.0	0.11677824003354137	2.3121351643388602E-4
82-83	6.011551427281037E-4	0.0011406533477405045	0.0	0.11742563787955626	2.620419852917375E-4
84-85	6.011551427281037E-4	0.0011406533477405045	0.0	0.1175797802238455	2.620419852917375E-4
86-87	6.011551427281037E-4	0.0011406533477405045	0.0	0.11765685139599014	2.620419852917375E-4
88	6.011551427281037E-4	0.001217724519885133	0.0	0.11765685139599014	2.620419852917375E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	9255	0.0	55.202522	1
GGTATCA	9445	0.0	50.973953	1
TATACAC	12685	0.0	42.186607	3
TTATACA	13000	0.0	40.055088	2
GTATCAA	17610	0.0	38.41591	1
ATCAACG	20945	0.0	31.987982	3
TCAACGC	21050	0.0	31.868967	4
TATCAAC	21710	0.0	31.101965	2
CAACGCA	21705	0.0	30.94657	5
AACGCAG	22460	0.0	29.906288	6
TACACAT	19020	0.0	28.427172	5
ACACATC	19785	0.0	26.31427	6
GTGGTAT	4290	0.0	26.16614	1
ATACACA	21680	0.0	25.864471	4
ACGCAGA	26080	0.0	25.755186	7
CGCAGAG	26345	0.0	25.496119	8
ACATCTC	20680	0.0	25.196064	8
TGGTATC	4265	0.0	24.718328	2
CACATCT	21760	0.0	23.729795	7
CATCTCC	22725	0.0	22.740908	9
>>END_MODULE