FastQCFastQC Report
Fri 27 May 2016
SRR2140133_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140133_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5743100
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72250.050.3477481
GTATCAA132550.039.0781141
ATCAACG162200.032.494743
TCAACGC162700.032.421654
CAACGCA166550.031.6983415
AACGCAG172350.030.6568856
TATCAAC173550.030.6494392
CTTATAC66300.028.7143761
ACGCAGA193650.027.239887
CGCAGAG197950.026.670168
TATACAC88250.024.6793543
GCAGAGT232450.022.7305419
TTATACA92700.021.8056832
GAGTACT156900.020.64127712-13
GTGGTAT35850.019.8075081
AGAGTAC213000.019.77540610-11
CAGAGTA229000.019.56353610-11
GTACATG141700.019.5224971
ACACATC101250.019.273546
TACATGG143500.019.156182
Produced by FastQC (version 0.11.5)