##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140133_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5743100
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.793423064198777	18.0	18.0	27.0	18.0	33.0
2	28.362539220978217	29.0	27.0	31.0	25.0	33.0
3	30.298200275112745	31.0	29.0	33.0	27.0	33.0
4	31.38450122756003	33.0	31.0	33.0	29.0	33.0
5	32.325309153593004	33.0	33.0	33.0	31.0	33.0
6	36.03532360571817	38.0	36.0	38.0	33.0	38.0
7	36.1518453448486	38.0	36.0	38.0	33.0	38.0
8	35.98952900001741	38.0	36.0	38.0	33.0	38.0
9	36.83355870522888	38.0	38.0	38.0	35.0	38.0
10-11	36.95587705246295	38.0	38.0	38.0	35.0	38.0
12-13	37.11172711601748	38.0	38.0	38.0	36.0	38.0
14-15	37.01387012240775	38.0	38.0	38.0	35.5	38.0
16-17	37.10753843742927	38.0	38.0	38.0	36.0	38.0
18-19	37.23587130643729	38.0	38.0	38.0	36.0	38.0
20-21	37.29469213703082	38.0	38.0	38.0	36.5	38.0
22-23	37.26556891114468	38.0	38.0	38.0	36.5	38.0
24-25	37.33804321096446	38.0	38.0	38.0	37.0	38.0
26-27	37.32322174355876	38.0	38.0	38.0	37.0	38.0
28-29	37.29940707181461	38.0	38.0	38.0	37.0	38.0
30-31	37.305039806219895	38.0	38.0	38.0	37.0	38.0
32-33	37.28679880719423	38.0	38.0	38.0	37.0	38.0
34-35	37.2034290317294	38.0	38.0	38.0	36.0	38.0
36-37	37.22192724980348	38.0	38.0	38.0	36.5	38.0
38-39	37.193755257613354	38.0	38.0	38.0	36.0	38.0
40-41	37.21501939320571	38.0	38.0	38.0	37.0	38.0
42-43	37.19574838887528	38.0	38.0	38.0	36.0	38.0
44-45	37.15733589667202	38.0	38.0	38.0	36.0	38.0
46-47	37.111217595586716	38.0	38.0	38.0	36.0	38.0
48-49	37.056123281728425	38.0	38.0	38.0	36.0	38.0
50-51	37.00655041234005	38.0	38.0	38.0	36.0	38.0
52-53	36.90346507229435	38.0	38.0	38.0	36.0	38.0
54-55	36.931087461905776	38.0	38.0	38.0	36.0	38.0
56-57	36.88740015055576	38.0	38.0	38.0	35.5	38.0
58-59	36.86268967955205	38.0	38.0	38.0	35.0	38.0
60-61	36.85488538049881	38.0	38.0	38.0	35.0	38.0
62-63	36.859174140893074	38.0	38.0	38.0	35.0	38.0
64-65	36.86467087675602	38.0	38.0	38.0	35.0	38.0
66-67	36.855923206921815	38.0	38.0	38.0	35.0	38.0
68-69	36.8360455068191	38.0	38.0	38.0	35.0	38.0
70-71	36.83229268295413	38.0	38.0	38.0	35.0	38.0
72-73	36.842369996674606	38.0	38.0	38.0	35.0	38.0
74-75	36.78025434894932	38.0	38.0	38.0	35.0	38.0
76-77	36.775900212626254	38.0	38.0	38.0	35.0	38.0
78-79	36.7755392106606	38.0	38.0	38.0	35.0	38.0
80-81	36.752540383844064	38.0	38.0	38.0	35.0	38.0
82-83	36.69479060756534	38.0	38.0	38.0	34.0	38.0
84-85	36.68087401277796	38.0	38.0	38.0	34.0	38.0
86-87	36.671457548020044	38.0	38.0	38.0	34.0	38.0
88-89	36.64990375061929	38.0	38.0	38.0	34.0	38.0
90-91	36.65364056516847	38.0	38.0	38.0	34.0	38.0
92-93	36.62300115795338	38.0	38.0	38.0	34.0	38.0
94-95	36.55091275589061	38.0	38.0	38.0	34.0	38.0
96-97	36.56348142242276	38.0	38.0	38.0	34.0	38.0
98-99	36.58572634151348	38.0	38.0	38.0	34.0	38.0
100	34.85737037013829	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	9.0
19	14.0
20	27.0
21	116.0
22	453.0
23	1292.0
24	3008.0
25	6198.0
26	11248.0
27	18945.0
28	29271.0
29	43382.0
30	58069.0
31	75399.0
32	99912.0
33	139775.0
34	218616.0
35	413414.0
36	1277763.0
37	3346185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.45642597203601	17.790531246191083	19.015322735108217	23.73772004666469
2	13.907859799720354	21.018617895254334	42.522914714404806	22.550607590620505
3	17.81466455398652	25.640263968936637	31.852988803956052	24.692082673120787
4	12.124027093381622	18.338858804478416	39.298479915028466	30.23863418711149
5	13.157458515435913	36.92201076073897	36.31777263150563	13.602758092319478
6	29.15254827532169	38.20072782991764	19.149065835524368	13.4976580592363
7	25.705420417544534	32.76249760582264	23.45816719193467	18.073914784698157
8	23.639933137155893	37.06517386080688	21.661332729710438	17.633560272326793
9	24.760425554143232	17.201824798453796	21.81647542268113	36.22127422472184
10-11	23.696026536191255	26.835411189079068	29.374275217217182	20.094287057512496
12-13	24.664022914453867	24.30509655064338	29.471731294945236	21.559149239957513
14-15	21.771212411415437	25.76549250404834	27.177926555344673	25.285368529191555
16-17	21.376181853006216	28.98788110950532	27.81307133777925	21.822865699709215
18-19	21.308561578241715	27.87885462555066	29.737432745381415	21.07515105082621
20-21	21.959290159173705	27.01634710851442	29.72216427762747	21.302198454684408
22-23	21.670288154456912	27.27405620824177	29.55059160544441	21.505064031856904
24-25	21.621283221293254	27.317260139811673	29.71857428524709	21.342882353647987
26-27	21.591004185831082	27.51087820597835	29.58204996089689	21.316067647293682
28-29	21.35227996955285	27.34791950127155	29.679222240600613	21.620578288574993
30-31	21.908376088340642	27.27026147194599	29.62039305989594	21.200969379817426
32-33	21.730832285547645	27.344095205820835	29.633886938287745	21.291185570343778
34-35	21.724040950029064	27.41378208493492	29.739022727684024	21.123154237351997
36-37	21.70314692878276	27.258461935445872	29.648257722875755	21.390133412895608
38-39	21.40202497160558	27.17426832400285	29.888738916409586	21.534967787981987
40-41	21.680028472512944	27.027584160644913	29.790647861000696	21.501739505841442
42-43	21.682814574418387	27.091524121470705	29.678609694922553	21.547051609188355
44-45	21.605551420765895	27.364772889363444	29.496613509080838	21.533062180789823
46-47	21.83250078956507	27.184273930335877	29.03502344435464	21.948201835744406
48-49	21.74023577930623	27.255291920466313	29.141662031572903	21.862810268654556
50-51	21.600756672768778	27.472249129923664	29.12054713933604	21.806447057971518
52-53	21.710236117636374	27.554108976962592	29.139439284748427	21.596215620652607
54-55	21.63625274385412	27.512394001454073	28.969666000921574	21.881687253770238
56-57	21.83980000429048	27.69022561285488	28.706385940825356	21.76358844202928
58-59	21.53523616849408	27.90683480272818	29.06920400351248	21.488725025265254
60-61	21.717392995937704	28.16553616011939	28.68858837142166	21.428482472521242
62-63	21.510145973310806	28.33832977616269	28.68832746169988	21.46319678882662
64-65	21.392781157525526	28.233751547846836	28.756383622233102	21.61708367239454
66-67	21.40363337656822	28.16705126386928	28.765813318297774	21.663502041264728
68-69	21.531131522670744	28.010564242102813	28.812344413834506	21.645959821391934
70-71	21.57678266228803	27.63926431356444	28.836398173468968	21.947554850678564
72-73	21.73102902128849	27.469548373131047	28.948400174868926	21.851022430711538
74-75	21.711996100642637	27.650964648380093	28.795626877066844	21.841412373910426
76-77	21.821278714636172	27.590687731440628	28.602414806600997	21.9856187473222
78-79	21.786576769621284	27.61276896084362	28.596713411436838	22.003940858098264
80-81	21.816475888565463	27.659414201697714	28.57068650128668	21.953423408450146
82-83	21.93036038407942	27.68398542683192	28.584894363395826	21.800759825692836
84-85	22.037545779029344	27.65099872218616	28.472298430188232	21.83915706859626
86-87	21.853082910398342	27.734916823334654	28.522737962330304	21.8892623039367
88-89	21.967256149021903	27.682765083944567	28.466088677401093	21.883890089632438
90-91	21.98306761024216	27.734024133825802	28.441186062728857	21.841722193203182
92-93	22.013480879603037	27.726628618415944	28.471283670747905	21.78860683123311
94-95	21.91484992164319	27.85574646718439	28.39542488360572	21.833978727566706
96-97	21.981581401454044	27.88759015890928	28.52516547402303	21.605662965613647
98-99	23.020801760780877	29.80479376102459	28.78015688711238	18.39424759108216
100	21.911159290427285	30.74861049320442	22.696125672822063	24.644104543546234
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1208.0
1	1169.5
2	1464.5
3	1952.0
4	2540.0
5	3512.0
6	4422.5
7	5963.5
8	7448.0
9	7812.5
10	7616.0
11	7534.0
12	8362.0
13	10457.5
14	14995.5
15	20370.0
16	24155.0
17	25990.0
18	25448.5
19	23312.0
20	20794.0
21	18864.0
22	18631.0
23	20944.0
24	26028.0
25	33608.5
26	43837.5
27	55839.5
28	68129.0
29	82074.5
30	96101.5
31	109920.0
32	124695.0
33	138610.5
34	152575.5
35	165448.5
36	178360.5
37	191934.0
38	201799.5
39	208593.5
40	216530.5
41	224489.0
42	229671.5
43	233586.5
44	240979.5
45	253472.0
46	255942.5
47	249947.0
48	244826.5
49	234689.5
50	224572.0
51	214510.0
52	201330.0
53	186787.0
54	171773.5
55	156330.0
56	140464.5
57	124296.0
58	107412.5
59	90186.5
60	73177.5
61	57787.5
62	44918.5
63	33986.0
64	24798.5
65	17888.0
66	12669.0
67	9254.5
68	7123.5
69	5278.0
70	3790.5
71	2662.0
72	1769.0
73	1135.5
74	714.5
75	450.5
76	278.0
77	164.5
78	99.0
79	64.5
80	39.5
81	24.0
82	19.5
83	13.0
84	6.5
85	5.0
86	4.0
87	2.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.014434712959899705
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.7414172942589477E-4
26-27	1.48035164098721E-4
28-29	0.0029178104193180996
30-31	0.00524668254093175
32-33	0.003089370514576549
34-35	0.001197702579151967
36-37	0.0
38-39	0.0
40-41	3.269238874360411E-4
42-43	0.0
44-45	8.926146226520776E-6
46-47	3.4143237891302847E-4
48-49	0.0010491028994490943
50-51	0.002347260869387215
52-53	0.0
54-55	0.0022891741827119542
56-57	0.03349892995691727
58-59	0.05411120051635793
60-61	0.03658032381074868
62-63	0.012376837272209165
64-65	2.563125266102241E-4
66-67	0.0
68-69	9.655115415318653E-6
70-71	0.0024764983717023206
72-73	0.004520230246150664
74-75	0.003324107529222626
76-77	0.0028695689907375912
78-79	0.0068135833824361555
80-81	0.014473355355736068
82-83	0.03681305527041608
84-85	0.015879734720611202
86-87	0.0015104903030689412
88-89	0.0010298486931587887
90-91	8.484748081996182E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	38.0
20-21	165.0
22-23	357.0
24-25	491.0
26-27	974.0
28-29	2375.0
30-31	7304.0
32-33	9232.0
34-35	12996.0
36-37	20470.0
38-39	23757.0
40-41	25077.0
42-43	29705.0
44-45	35881.0
46-47	37021.0
48-49	33615.0
50-51	29751.0
52-53	27932.0
54-55	29679.0
56-57	31097.0
58-59	33720.0
60-61	37843.0
62-63	37087.0
64-65	41030.0
66-67	45349.0
68-69	46464.0
70-71	56498.0
72-73	38955.0
74-75	37780.0
76-77	39542.0
78-79	41195.0
80-81	41131.0
82-83	39322.0
84-85	39421.0
86-87	41065.0
88-89	43357.0
90-91	44459.0
92-93	45194.0
94-95	54292.0
96-97	181696.0
98-99	410058.0
100-101	3989725.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.95153396789729
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.82348960228188	77.204667176656
2	7.280032547257613	12.51459929546021
3	1.6948557528570247	4.3702635543713
4	0.6111411401317981	2.101140738608709
5	0.2514505072259243	1.0806278406537018
6	0.12277714989102542	0.6331730621604053
7	0.07149138691074061	0.4301356059329426
8	0.04045491556868212	0.27817296397359986
9	0.025611759597889774	0.1981233022550078
>10	0.07667605707262584	0.988555570095666
>50	0.0013035620382131075	0.07311260937142058
>100	6.851781565736495E-4	0.11060449727085682
>500	3.0441010023267944E-5	0.016823783190215862
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.223659695983006E-5	0.0	0.0	0.0
3	0.0	6.964879594644007E-5	0.0	0.0	0.0
4	0.0	1.3929759189288015E-4	0.0	0.0	1.7412198986610018E-5
5	0.0	1.5670979087949017E-4	0.0	0.0	1.7412198986610018E-5
6	8.70609949330501E-5	1.5670979087949017E-4	0.0	0.0	1.7412198986610018E-5
7	8.70609949330501E-5	1.5670979087949017E-4	0.0	0.0	1.7412198986610018E-5
8	8.70609949330501E-5	1.5670979087949017E-4	0.0	0.0	1.7412198986610018E-5
9	8.70609949330501E-5	2.6118298479915026E-4	0.0	1.7412198986610018E-5	3.4824397973220036E-5
10-11	9.57670944263551E-5	2.6118298479915026E-4	0.0	4.3530497466525044E-5	3.4824397973220036E-5
12-13	1.0447319391966012E-4	2.6118298479915026E-4	0.0	5.223659695983006E-5	3.4824397973220036E-5
14-15	1.0447319391966012E-4	2.785951837857603E-4	0.0	5.223659695983006E-5	1.0447319391966012E-4
16-17	1.5670979087949017E-4	2.960073827723703E-4	0.0	5.223659695983006E-5	1.2188539290627013E-4
18-19	1.654158903727952E-4	3.0471348226567533E-4	0.0	5.223659695983006E-5	1.3929759189288015E-4
20-21	2.0894638783932023E-4	3.1341958175898035E-4	0.0	8.70609949330501E-5	1.5670979087949017E-4
22-23	2.2635858682593026E-4	4.0918667618533545E-4	0.0	8.70609949330501E-5	1.828280893594052E-4
24-25	2.4377078581254026E-4	4.440110741585555E-4	0.0	1.1317929341296513E-4	1.915341888527102E-4
26-27	2.6118298479915026E-4	4.527171736518605E-4	0.0	1.5670979087949017E-4	1.915341888527102E-4
28-29	2.6118298479915026E-4	4.527171736518605E-4	0.0	3.482439797322004E-4	1.915341888527102E-4
30-31	2.785951837857603E-4	5.049537706116906E-4	0.0	0.0012449722275426163	2.0024028834601522E-4
32-33	3.1341958175898035E-4	5.049537706116906E-4	0.0	0.002942661628737093	2.2635858682593026E-4
34-35	3.830683777054204E-4	6.268391635179606E-4	0.0	0.005188835298009786	2.4377078581254026E-4
36-37	3.830683777054204E-4	7.139001584510107E-4	0.0	0.008775748289251449	2.4377078581254026E-4
38-39	3.830683777054204E-4	7.313123574376208E-4	0.0	0.020581219202173043	2.4377078581254026E-4
40-41	3.830683777054204E-4	8.009611533840609E-4	0.0	0.03416273441172886	2.4377078581254026E-4
42-43	3.830683777054204E-4	8.096672528773659E-4	0.0	0.04131914819522557	2.4377078581254026E-4
44-45	3.917744771987254E-4	8.61903849837196E-4	0.0	0.04975535860423813	2.5247688530584525E-4
46-47	4.1789277567864046E-4	9.05434347303721E-4	0.0	0.05919277045498076	2.698890842924553E-4
48-49	4.1789277567864046E-4	9.05434347303721E-4	0.0	0.06789886994828576	2.785951837857603E-4
50-51	4.527171736518605E-4	9.924953422367712E-4	0.0	0.07762358308230746	2.785951837857603E-4
52-53	4.527171736518605E-4	9.924953422367712E-4	0.0	0.10883494976580593	3.1341958175898035E-4
54-55	4.527171736518605E-4	0.0010099075412233812	0.0	0.14888300743500898	3.5695007922550536E-4
56-57	4.527171736518605E-4	0.001062144138183211	0.0	0.20444533440128154	3.656561787188104E-4
58-59	4.7012937263847055E-4	0.001062144138183211	0.0	0.23926102627500828	3.656561787188104E-4
60-61	5.310720690916055E-4	0.001079556337169821	0.0	0.2522505267190194	3.656561787188104E-4
62-63	5.397781685849106E-4	0.0011143807351430412	0.0	0.26315926938413053	4.0048057669203043E-4
64-65	5.397781685849106E-4	0.0013059149239957515	0.0	0.2690271804426181	4.0048057669203043E-4
66-67	5.397781685849106E-4	0.0013059149239957515	0.0	0.2741028364472149	4.0918667618533545E-4
68-69	5.571903675715206E-4	0.0013320332224756666	0.0	0.27938743883965106	4.1789277567864046E-4
70-71	5.746025665581306E-4	0.0013407393219689715	0.0	0.2832790653131584	4.1789277567864046E-4
72-73	6.268391635179607E-4	0.0013407393219689715	0.0	0.28631749403632184	5.223659695983005E-4
74-75	6.268391635179607E-4	0.0013929759189288014	0.0	0.28892932388431336	5.223659695983005E-4
76-77	6.268391635179607E-4	0.0014626247148752415	0.0	0.29121032195155927	5.223659695983005E-4
78-79	6.268391635179607E-4	0.0015148613118350716	0.0	0.2930560150441399	5.223659695983005E-4
80-81	6.442513625045707E-4	0.0015148613118350716	0.0	0.29344778952133865	5.397781685849106E-4
82-83	6.442513625045707E-4	0.0015148613118350716	0.0	0.29386568229701726	5.658964670648256E-4
84-85	6.442513625045707E-4	0.0015322735108216816	0.0	0.2941094530828298	5.746025665581306E-4
86-87	6.442513625045707E-4	0.0015670979087949017	0.0	0.2942661628737093	5.746025665581306E-4
88	6.442513625045707E-4	0.0016541589037279518	0.0	0.2942835750726959	5.920147655447406E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7225	0.0	50.347748	1
GTATCAA	13255	0.0	39.078114	1
ATCAACG	16220	0.0	32.49474	3
TCAACGC	16270	0.0	32.42165	4
CAACGCA	16655	0.0	31.698341	5
AACGCAG	17235	0.0	30.656885	6
TATCAAC	17355	0.0	30.649439	2
CTTATAC	6630	0.0	28.714376	1
ACGCAGA	19365	0.0	27.23988	7
CGCAGAG	19795	0.0	26.67016	8
TATACAC	8825	0.0	24.679354	3
GCAGAGT	23245	0.0	22.730541	9
TTATACA	9270	0.0	21.805683	2
GAGTACT	15690	0.0	20.641277	12-13
GTGGTAT	3585	0.0	19.807508	1
AGAGTAC	21300	0.0	19.775406	10-11
CAGAGTA	22900	0.0	19.563536	10-11
GTACATG	14170	0.0	19.522497	1
ACACATC	10125	0.0	19.27354	6
TACATGG	14350	0.0	19.15618	2
>>END_MODULE