FastQCFastQC Report
Fri 27 May 2016
SRR2140132_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140132_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6137550
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73050.048.671791
GTATCAA140250.038.4936181
TCAACGC171400.032.205094
ATCAACG171950.032.1528743
CAACGCA176400.031.341765
TATCAAC181450.030.7161562
AACGCAG183200.030.178426
ACGCAGA209000.026.4321487
CGCAGAG212850.025.9745668
TGGTATC36450.023.2475592
GTGGTAT38650.022.7153661
CTTATAC50750.022.463511
GCAGAGT250150.021.9792799
AGAGTAC222350.019.97432110-11
GAGTACT148350.019.84086412-13
TACATGG179500.019.491322
GTACATG182050.019.3382661
CAGAGTA241900.019.06409810-11
TATACAC75750.018.9094733
ACATGGG181200.018.702163
Produced by FastQC (version 0.11.5)