##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140132_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6137550
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.542816922061736	18.0	18.0	25.0	18.0	33.0
2	28.1433806649233	29.0	27.0	31.0	25.0	33.0
3	30.126159623954184	31.0	29.0	33.0	27.0	33.0
4	31.262682177741933	33.0	31.0	33.0	29.0	33.0
5	32.26508639440819	33.0	33.0	33.0	31.0	33.0
6	35.85267297211428	38.0	36.0	38.0	31.0	38.0
7	35.98881100764963	38.0	36.0	38.0	32.0	38.0
8	35.77378937849794	38.0	36.0	38.0	31.0	38.0
9	36.695962884212754	38.0	37.0	38.0	34.0	38.0
10-11	36.84294531205448	38.0	38.0	38.0	34.5	38.0
12-13	37.01396045653396	38.0	38.0	38.0	35.5	38.0
14-15	36.92126117098842	38.0	38.0	38.0	35.0	38.0
16-17	37.02780767570122	38.0	38.0	38.0	35.5	38.0
18-19	37.16586072618553	38.0	38.0	38.0	36.0	38.0
20-21	37.23283201718762	38.0	38.0	38.0	36.0	38.0
22-23	37.218561981856574	38.0	38.0	38.0	36.0	38.0
24-25	37.29093490773714	38.0	38.0	38.0	36.5	38.0
26-27	37.27546920334324	38.0	38.0	38.0	37.0	38.0
28-29	37.2562092961866	38.0	38.0	38.0	36.5	38.0
30-31	37.26591366972242	38.0	38.0	38.0	37.0	38.0
32-33	37.250123040142554	38.0	38.0	38.0	36.5	38.0
34-35	37.16533036412974	38.0	38.0	38.0	36.0	38.0
36-37	37.19100061643617	38.0	38.0	38.0	36.0	38.0
38-39	37.177650001623334	38.0	38.0	38.0	36.0	38.0
40-41	37.17325426408472	38.0	38.0	38.0	36.0	38.0
42-43	37.15355799367775	38.0	38.0	38.0	36.0	38.0
44-45	37.117270781226836	38.0	38.0	38.0	36.0	38.0
46-47	37.07095909250714	38.0	38.0	38.0	36.0	38.0
48-49	37.01291774080451	38.0	38.0	38.0	36.0	38.0
50-51	36.95991841696333	38.0	38.0	38.0	36.0	38.0
52-53	36.857856168215704	38.0	38.0	38.0	35.5	38.0
54-55	36.88063094940284	38.0	38.0	38.0	35.5	38.0
56-57	36.82875925152645	38.0	38.0	38.0	35.0	38.0
58-59	36.80283371808539	38.0	38.0	38.0	35.0	38.0
60-61	36.79491651937092	38.0	38.0	38.0	35.0	38.0
62-63	36.79501687612789	38.0	38.0	38.0	35.0	38.0
64-65	36.79601032241442	38.0	38.0	38.0	35.0	38.0
66-67	36.786200537726586	38.0	38.0	38.0	35.0	38.0
68-69	36.76284899202818	38.0	38.0	38.0	35.0	38.0
70-71	36.75503591025445	38.0	38.0	38.0	35.0	38.0
72-73	36.76273678894778	38.0	38.0	38.0	35.0	38.0
74-75	36.69561618507059	38.0	38.0	38.0	34.5	38.0
76-77	36.68785602829166	38.0	38.0	38.0	34.0	38.0
78-79	36.68426282252058	38.0	38.0	38.0	34.0	38.0
80-81	36.65959338432977	38.0	38.0	38.0	34.0	38.0
82-83	36.595926108199365	38.0	38.0	38.0	34.0	38.0
84-85	36.58048270220113	38.0	38.0	38.0	34.0	38.0
86-87	36.56553768187058	38.0	38.0	38.0	34.0	38.0
88-89	36.53801500278185	38.0	38.0	38.0	34.0	38.0
90-91	36.54219942257164	38.0	38.0	38.0	34.0	38.0
92-93	36.50632613100815	38.0	38.0	38.0	34.0	38.0
94-95	36.43566695097243	38.0	37.5	38.0	34.0	38.0
96-97	36.44684074316814	38.0	37.0	38.0	34.0	38.0
98-99	36.471857591423635	38.0	37.0	38.0	34.0	38.0
100	34.737714004216464	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	7.0
19	15.0
20	36.0
21	154.0
22	562.0
23	1545.0
24	3774.0
25	7552.0
26	13148.0
27	21646.0
28	33336.0
29	48300.0
30	65133.0
31	85945.0
32	114151.0
33	161882.0
34	253669.0
35	479329.0
36	1442286.0
37	3405080.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.883227020553804	16.96036692165441	19.63845508386897	23.51795097392282
2	14.334612644452108	20.98574854366464	41.84460346026715	22.8350353516161
3	18.329854746600844	25.30038859153897	31.303451703041112	25.066304958819074
4	12.585314987250612	17.86743896180072	38.666112699692874	30.881133351255798
5	13.637982582626618	36.9066810046354	35.60761215794576	13.84772425479222
6	30.105612174238907	37.695350750706716	18.643286001743366	13.555751073311011
7	26.637892970322035	32.564964847536885	22.832221326099177	17.964920856041903
8	24.35251851308747	36.86156528256389	21.1115347329146	17.674381471434042
9	25.52624418538342	17.183354921752166	21.110948179648233	36.179452713216186
10-11	24.341773183110522	26.76375752539694	28.696279460045133	20.1981898314474
12-13	25.180112585640853	24.131062068740782	28.94760938811089	21.741215957507475
14-15	22.26714242653828	25.54328681640068	26.663636141457097	25.525934615603944
16-17	21.888652638267715	28.759781997702667	27.253187346742592	22.09837801728703
18-19	21.88638789093368	27.718307793826526	29.12544907984456	21.26985523539523
20-21	22.64831496601826	26.883403021187412	29.040197637877064	21.428084374917262
22-23	22.325041535300137	26.836964995475583	28.97807886089689	21.859914608327394
24-25	22.14241543075543	26.944451007726293	29.10441620151104	21.808717360007236
26-27	22.019477200372247	27.11952891761363	29.036937289809583	21.824056592204542
28-29	21.836739185008167	27.104151248504373	29.07142181809883	21.98768774838863
30-31	22.21717366348787	26.854148443123172	29.088050045163804	21.840627848225154
32-33	21.97107434338178	27.08655148964347	29.034983079184222	21.90739108779053
34-35	22.10299087956719	27.233817216009275	29.02641592661899	21.636775977804543
36-37	22.150531249518156	27.054292201505746	28.910737548926562	21.88443900004954
38-39	22.002154910183812	27.137930881189998	29.04418998312525	21.815724225500947
40-41	22.28125616439194	26.990128209431504	28.920842634865114	21.80777299131144
42-43	22.22355179661531	27.044282217914994	28.810816022177853	21.921349963291846
44-45	22.189792552155847	27.206569187297514	28.717626871737806	21.88601138880883
46-47	22.339127435663368	27.086588901319057	28.344624810274198	22.229658852743373
48-49	22.20357669466878	27.214956779351485	28.430875708507504	22.150590817472235
50-51	22.162027519800144	27.38368172680406	28.378402397031216	22.07588835636458
52-53	22.325089749897096	27.351340446075902	28.294920790559924	22.02864901346708
54-55	22.256504732507477	27.280565365801806	28.26278162868793	22.20014827300279
56-57	22.340131963544295	27.457095249350445	28.104619519407315	22.098153267697946
58-59	22.161024331945985	27.59100765054816	28.32085033810341	21.927117679402443
60-61	22.336753576611386	27.635739583004177	28.159459320118437	21.868047520266
62-63	22.154270706660043	27.773212022321598	28.142760775268506	21.929756495749857
64-65	22.112606927064576	27.679147847328974	28.19594703422189	22.012298191384563
66-67	22.235139759850465	27.68195476738834	28.056228044290656	22.02667742847054
68-69	22.21805866210235	27.658136917834632	28.195524142874305	21.928280277188712
70-71	22.212357788546257	27.231431262561685	28.254102042700218	22.302108906191837
72-73	22.2628104401351	27.224103689144947	28.300459409518936	22.21262646120102
74-75	22.160132524689768	27.40614125491232	28.212703823843377	22.221022396554535
76-77	22.280028938046897	27.302049319931736	28.055531242293704	22.36239049972766
78-79	22.25680863874434	27.420083334613466	28.00564930897314	22.31745871766906
80-81	22.29121693813401	27.38792567387136	27.979208195661652	22.341649192332973
82-83	22.340652264195686	27.35635202018898	28.0375909999435	22.26540471567183
84-85	22.45261818889616	27.389520061679345	27.932450400951268	22.225411348473223
86-87	22.240146495437795	27.42656786459627	27.978568575543676	22.354717064422253
88-89	22.35844898101078	27.42109178490538	27.93064010322992	22.28981913085392
90-91	22.365861079028626	27.402962709931387	27.989682049800184	22.241494161239803
92-93	22.39606531503814	27.397901354112392	27.93673479925293	22.269298531596537
94-95	22.319940900547206	27.527467238368665	27.87047235953078	22.28211950155335
96-97	22.31187343363188	27.66669695263041	27.98563772297276	22.03579189076495
98-99	23.40269832801264	29.551407569112158	28.180995323513812	18.86489877936139
100	22.33871325477854	30.405466927841502	22.170054701964183	25.085765115415775
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1533.0
1	1378.5
2	1552.0
3	2010.0
4	2409.0
5	3128.0
6	3985.5
7	5879.5
8	7769.5
9	8247.0
10	7982.0
11	7765.5
12	8402.5
13	10191.5
14	14035.0
15	18600.0
16	21952.0
17	23496.5
18	22881.0
19	20973.5
20	18758.0
21	17009.0
22	17060.5
23	19377.5
24	24125.0
25	31811.5
26	42449.5
27	54625.5
28	65715.5
29	77472.5
30	90343.5
31	102737.5
32	116954.0
33	132513.5
34	147727.0
35	162340.5
36	178929.0
37	193893.5
38	203220.0
39	210908.5
40	221396.5
41	231173.5
42	237423.0
43	246523.5
44	256541.5
45	262509.0
46	263703.0
47	262359.0
48	258875.5
49	252965.5
50	246570.5
51	238353.5
52	227590.5
53	215331.0
54	202251.5
55	187632.0
56	171008.5
57	152925.0
58	135199.5
59	116672.0
60	96677.0
61	78096.0
62	61045.5
63	46255.0
64	34034.0
65	24607.0
66	18166.5
67	13879.5
68	10522.5
69	7546.5
70	5290.0
71	3637.5
72	2537.5
73	1648.5
74	1005.5
75	597.0
76	343.0
77	202.5
78	120.5
79	76.0
80	51.5
81	34.5
82	22.5
83	17.0
84	12.5
85	7.5
86	5.5
87	6.5
88	5.5
89	3.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.014891935707244747
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.9107954085306504E-4
26-27	3.3407906298808137E-4
28-29	0.003952870374785088
30-31	0.006075844481216669
32-33	0.004213795230620768
34-35	0.002028794151837245
36-37	0.0
38-39	8.232388471922987E-6
40-41	4.960424903383324E-4
42-43	0.0
44-45	8.349076212287953E-6
46-47	3.2767370823462514E-4
48-49	0.0014288312270472848
50-51	0.002797807471387529
52-53	0.0
54-55	0.003153731070094466
56-57	0.038274863992012145
58-59	0.05983697011941835
60-61	0.04101803472341132
62-63	0.013561845140108391
64-65	2.7479341297141917E-4
66-67	0.0
68-69	2.6997140462882173E-5
70-71	0.0030678546828033404
72-73	0.005025468782868076
74-75	0.004232510717058318
76-77	0.0030104177177577296
78-79	0.007399230442569515
80-81	0.015394628068147015
82-83	0.03958027763145752
84-85	0.018519962195851145
86-87	0.0016069631455587219
88-89	0.001259695382220386
90-91	6.898865392886442E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	34.0
20-21	175.0
22-23	397.0
24-25	474.0
26-27	1130.0
28-29	2697.0
30-31	7713.0
32-33	9932.0
34-35	13658.0
36-37	21435.0
38-39	25665.0
40-41	25992.0
42-43	30454.0
44-45	37102.0
46-47	37789.0
48-49	35114.0
50-51	31550.0
52-53	29779.0
54-55	32545.0
56-57	33572.0
58-59	36061.0
60-61	37332.0
62-63	36913.0
64-65	39624.0
66-67	43228.0
68-69	45138.0
70-71	49842.0
72-73	40161.0
74-75	40147.0
76-77	42638.0
78-79	43929.0
80-81	44634.0
82-83	42345.0
84-85	42512.0
86-87	44175.0
88-89	46336.0
90-91	47709.0
92-93	48398.0
94-95	58382.0
96-97	197369.0
98-99	450260.0
100-101	4283210.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.46435135671989
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.48062799575301	78.23348810001455
2	6.835661218633985	11.820820267269458
3	1.5384318782200017	3.9905854337037825
4	0.5458269072713231	1.8877827796103783
5	0.23222213051032106	1.003946794262523
6	0.13045945005598283	0.6768055036466971
7	0.0678777020930658	0.41083010381431223
8	0.04231495829114951	0.2926988337064719
9	0.03385453268114166	0.263448918788381
>10	0.09033082008886957	1.1703139367961797
>50	0.0016347046160204697	0.09754210671630603
>100	7.294425898967479E-4	0.11533036820104715
>500	0.0	0.0
>1k	2.8259195219969196E-5	0.03640685346990758
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.887943886404184E-5	0.0	0.0	0.0
3	0.0	4.887943886404184E-5	0.0	0.0	0.0
4	0.0	1.1405202401609763E-4	0.0	0.0	1.6293146288013947E-5
5	0.0	1.1405202401609763E-4	0.0	0.0	1.6293146288013947E-5
6	0.0	1.1405202401609763E-4	0.0	0.0	1.6293146288013947E-5
7	0.0	1.1405202401609763E-4	0.0	1.6293146288013947E-5	1.6293146288013947E-5
8	0.0	1.1405202401609763E-4	0.0	1.6293146288013947E-5	1.6293146288013947E-5
9	0.0	1.3034517030411158E-4	0.0	3.2586292576027895E-5	1.6293146288013947E-5
10-11	0.0	1.3034517030411158E-4	0.0	3.2586292576027895E-5	1.6293146288013947E-5
12-13	0.0	1.3034517030411158E-4	0.0	5.702601200804882E-5	2.4439719432020923E-5
14-15	0.0	1.8737118231216038E-4	0.0	6.517258515205579E-5	3.2586292576027895E-5
16-17	0.0	1.9551775545616735E-4	0.0	1.0590545087209066E-4	3.2586292576027895E-5
18-19	0.0	2.199574748881883E-4	0.0	1.6293146288013947E-4	3.2586292576027895E-5
20-21	0.0	2.443971943202092E-4	0.0	1.6293146288013947E-4	3.2586292576027895E-5
22-23	0.0	2.9327663318425105E-4	0.0	1.9551775545616735E-4	4.073286572003487E-5
24-25	0.0	3.584492183363068E-4	0.0	2.3625062117620224E-4	4.887943886404184E-5
26-27	0.0	3.910355109123347E-4	0.0	3.01423206328258E-4	5.702601200804882E-5
28-29	0.0	3.910355109123347E-4	0.0	4.725012423524045E-4	6.517258515205579E-5
30-31	0.0	5.213806812164463E-4	0.0	0.001197546252169025	7.331915829606276E-5
32-33	8.146573144006974E-6	5.213806812164463E-4	0.0	0.0028920334661224753	9.775887772808368E-5
34-35	1.6293146288013947E-5	5.946998395125091E-4	0.0	0.005124194507580386	9.775887772808368E-5
36-37	1.6293146288013947E-5	6.517258515205579E-4	0.0	0.008790152422383525	9.775887772808368E-5
38-39	1.6293146288013947E-5	6.761655709525788E-4	0.0	0.01792246091681534	9.775887772808368E-5
40-41	1.6293146288013947E-5	7.820710218246694E-4	0.0	0.028016065042239982	1.0590545087209066E-4
42-43	1.6293146288013947E-5	8.146573144006973E-4	0.0	0.034248193497405315	1.221985971601046E-4
44-45	1.6293146288013947E-5	9.042696189847741E-4	0.0	0.04224812832482017	1.3034517030411158E-4
46-47	1.6293146288013947E-5	9.612956309928229E-4	0.0	0.050296942591099056	1.4663831659212552E-4
48-49	1.6293146288013947E-5	9.612956309928229E-4	0.0	0.0582968774185139	1.792246091681534E-4
50-51	1.6293146288013947E-5	0.0010101750698568647	0.0	0.06625607938020872	1.792246091681534E-4
52-53	1.6293146288013947E-5	0.0010101750698568647	0.0	0.08800742967470734	2.0366432860017435E-4
54-55	1.6293146288013947E-5	0.0010101750698568647	0.0	0.11507034565909849	2.2810404803219527E-4
56-57	1.6293146288013947E-5	0.0010264682161448787	0.0	0.15002729102003243	2.3625062117620224E-4
58-59	1.6293146288013947E-5	0.0010264682161448787	0.0	0.17831219297602463	2.443971943202092E-4
60-61	1.6293146288013947E-5	0.0010509079355768996	0.0	0.18730600972700834	2.6069034060822316E-4
62-63	1.6293146288013947E-5	0.0010753476550089205	0.0	0.19830388347141775	2.6069034060822316E-4
64-65	1.6293146288013947E-5	0.0013034517030411157	0.0	0.20354213000301424	2.6069034060822316E-4
66-67	1.6293146288013947E-5	0.0013115982761851227	0.0	0.2081775301219542	2.6069034060822316E-4
68-69	1.6293146288013947E-5	0.0013197448493291297	0.0	0.2125115070345659	2.6069034060822316E-4
70-71	1.6293146288013947E-5	0.0013278914224731366	0.0	0.21642186214368925	2.9327663318425105E-4
72-73	1.6293146288013947E-5	0.0013360379956171436	0.0	0.21954199965784393	3.2586292576027893E-4
74-75	1.6293146288013947E-5	0.0013604777150491645	0.0	0.221961531881614	3.2586292576027893E-4
76-77	1.6293146288013947E-5	0.0014012105807691994	0.0	0.224283305227656	3.2586292576027893E-4
78-79	1.6293146288013947E-5	0.0014175037270572133	0.0	0.22611628418505755	3.2586292576027893E-4
80-81	1.6293146288013947E-5	0.0014256503002012203	0.0	0.22671913059771406	3.2586292576027893E-4
82-83	1.6293146288013947E-5	0.0014337968733452273	0.0	0.22719163184006647	3.2586292576027893E-4
84-85	1.6293146288013947E-5	0.0014663831659212552	0.0	0.22746861532696272	3.2586292576027893E-4
86-87	1.6293146288013947E-5	0.001498969458497283	0.0	0.22761525364355484	3.2586292576027893E-4
88	1.6293146288013947E-5	0.001547848897361325	0.0	0.22763154678984285	3.2586292576027893E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7305	0.0	48.67179	1
GTATCAA	14025	0.0	38.493618	1
TCAACGC	17140	0.0	32.20509	4
ATCAACG	17195	0.0	32.152874	3
CAACGCA	17640	0.0	31.34176	5
TATCAAC	18145	0.0	30.716156	2
AACGCAG	18320	0.0	30.17842	6
ACGCAGA	20900	0.0	26.432148	7
CGCAGAG	21285	0.0	25.974566	8
TGGTATC	3645	0.0	23.247559	2
GTGGTAT	3865	0.0	22.715366	1
CTTATAC	5075	0.0	22.46351	1
GCAGAGT	25015	0.0	21.979279	9
AGAGTAC	22235	0.0	19.974321	10-11
GAGTACT	14835	0.0	19.840864	12-13
TACATGG	17950	0.0	19.49132	2
GTACATG	18205	0.0	19.338266	1
CAGAGTA	24190	0.0	19.064098	10-11
TATACAC	7575	0.0	18.909473	3
ACATGGG	18120	0.0	18.70216	3
>>END_MODULE