FastQCFastQC Report
Fri 27 May 2016
SRR2140131_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140131_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5710751
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69400.049.9040221
GTATCAA128650.038.033411
ATCAACG154050.032.2405323
TCAACGC155150.032.0119484
CAACGCA158900.031.2564775
TATCAAC163450.030.6535722
AACGCAG164150.030.2832726
CTTATAC45650.029.7947831
ACGCAGA189700.026.1587187
CGCAGAG191800.025.8949628
TATACAC69550.023.4913713
TGGTATC32950.022.1559582
GTGGTAT33900.021.791441
GCAGAGT228800.021.6124469
TTATACA74700.020.883852
GAGTACT142750.020.50119412-13
TACATGG151800.020.238782
GTACATG153900.019.6237891
ACATGGG155450.019.4272673
ACATCTC75350.019.318768
Produced by FastQC (version 0.11.5)