##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140131_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5710751
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.840004755941905	18.0	18.0	30.0	18.0	33.0
2	30.03712716593667	31.0	27.0	33.0	27.0	33.0
3	31.312745206366028	33.0	31.0	33.0	29.0	33.0
4	32.44330456712261	33.0	33.0	33.0	31.0	34.0
5	33.039356995253335	33.0	33.0	34.0	33.0	34.0
6	37.01362447776133	38.0	38.0	38.0	35.0	38.0
7	37.159658335654974	38.0	38.0	38.0	36.0	38.0
8	37.13070382511862	38.0	38.0	38.0	36.0	38.0
9	37.36142601909977	38.0	38.0	38.0	37.0	38.0
10-11	37.37842553457505	38.0	38.0	38.0	37.0	38.0
12-13	37.43473923132001	38.0	38.0	38.0	37.0	38.0
14-15	37.40164699879228	38.0	38.0	38.0	37.0	38.0
16-17	37.417112477850985	38.0	38.0	38.0	37.0	38.0
18-19	37.454352063327576	38.0	38.0	38.0	37.0	38.0
20-21	37.469647577352276	38.0	38.0	38.0	37.0	38.0
22-23	37.48995275180056	38.0	38.0	38.0	37.0	38.0
24-25	37.50001334613164	38.0	38.0	38.0	37.0	38.0
26-27	37.470238362111616	38.0	38.0	38.0	37.0	38.0
28-29	37.44633343544416	38.0	38.0	38.0	37.0	38.0
30-31	37.4448778333625	38.0	38.0	38.0	37.0	38.0
32-33	37.4332896267549	38.0	38.0	38.0	37.0	38.0
34-35	37.4016791124732	38.0	38.0	38.0	37.0	38.0
36-37	37.374743176818086	38.0	38.0	38.0	37.0	38.0
38-39	37.34726600689686	38.0	38.0	38.0	37.0	38.0
40-41	37.356580366164295	38.0	38.0	38.0	37.0	38.0
42-43	37.32479272010326	38.0	38.0	38.0	37.0	38.0
44-45	37.28088986391187	38.0	38.0	38.0	37.0	38.0
46-47	37.24369577431214	38.0	38.0	38.0	37.0	38.0
48-49	37.19482124193275	38.0	38.0	38.0	37.0	38.0
50-51	37.15385943115634	38.0	38.0	38.0	37.0	38.0
52-53	37.111771460020265	38.0	38.0	38.0	36.0	38.0
54-55	37.093072513749405	38.0	38.0	38.0	36.0	38.0
56-57	37.04715149394923	38.0	38.0	38.0	36.0	38.0
58-59	37.01553885820424	38.0	38.0	38.0	36.0	38.0
60-61	37.03398770987201	38.0	38.0	38.0	36.0	38.0
62-63	37.04447131149019	38.0	38.0	38.0	36.0	38.0
64-65	37.050372183300446	38.0	38.0	38.0	36.0	38.0
66-67	37.04021283074148	38.0	38.0	38.0	36.0	38.0
68-69	37.00024063160541	38.0	38.0	38.0	36.0	38.0
70-71	36.99722381078453	38.0	38.0	38.0	36.0	38.0
72-73	37.00821815418538	38.0	38.0	38.0	36.0	38.0
74-75	37.01964320997293	38.0	38.0	38.0	36.0	38.0
76-77	36.99959227314323	38.0	38.0	38.0	36.0	38.0
78-79	36.95655749719556	38.0	38.0	38.0	36.0	38.0
80-81	36.90827535952421	38.0	38.0	38.0	35.5	38.0
82-83	36.865688178500136	38.0	38.0	38.0	35.0	38.0
84-85	36.83744413962138	38.0	38.0	38.0	35.0	38.0
86-87	36.85547427180359	38.0	38.0	38.0	35.0	38.0
88-89	36.81060339895326	38.0	38.0	38.0	35.0	38.0
90-91	36.82022898554949	38.0	38.0	38.0	35.0	38.0
92-93	36.80676469456791	38.0	38.0	38.0	35.0	38.0
94-95	36.82131099900997	38.0	38.0	38.0	35.0	38.0
96-97	36.81737202197398	38.0	38.0	38.0	35.0	38.0
98-99	36.796626133106145	38.0	38.0	38.0	35.0	38.0
100	35.175834002268004	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	8.0
19	5.0
20	27.0
21	90.0
22	336.0
23	1031.0
24	2216.0
25	4819.0
26	8584.0
27	14502.0
28	23516.0
29	34628.0
30	46210.0
31	60062.0
32	77878.0
33	104879.0
34	157611.0
35	289846.0
36	913934.0
37	3970568.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.213029774893	19.609382373701813	17.055935375224728	23.121652476180454
2	14.374726262528442	20.79916193647732	42.315640822813904	22.510470978180333
3	18.197154518881007	25.655979024781207	30.93988361226104	25.206982844076748
4	12.468434260522482	18.099249111448838	38.653118067291565	30.779198560737115
5	13.41051290802208	37.175933603128556	35.783192088045865	13.630361400803501
6	29.94385502011907	37.76767714088742	18.694914206555318	13.593553632438185
7	26.484292521246328	32.55228603033121	22.844613606861863	18.118807841560596
8	24.073366182486332	37.0491026486709	21.186162730611088	17.69136843823168
9	25.075598638427763	17.354670165097374	21.36741735018739	36.20231384628747
10-11	24.125697303209332	26.846136348791955	28.84945430119436	20.17871204680435
12-13	25.01649958122846	24.16099038462717	29.072629852010706	21.749880182133662
14-15	22.03249625136869	25.65783379453946	26.771417629660267	25.53825232443159
16-17	21.679652991349123	28.887584137357763	27.424291481102927	22.008471390190188
18-19	21.619205600104085	27.792710625975463	29.315811528116004	21.272272245804448
20-21	22.354230913831298	26.97150766115426	29.306303105419957	21.36795831959449
22-23	22.044819349686893	27.01158221375436	29.215661380479403	21.727937056079348
24-25	21.87197604963449	27.143263062174018	29.365913372564695	21.6188475156268
26-27	21.896625767300964	27.291429348062152	29.26848598086319	21.5434589037737
28-29	21.714025188193187	27.165373504571473	29.450171068790148	21.670430238445192
30-31	22.151608665901623	27.0773095881531	29.216544204359913	21.55453754158537
32-33	21.868793330675327	27.125874885043196	29.241562835112422	21.76376894916906
34-35	21.843750978077352	27.286063492297934	29.36344703823232	21.50673849139239
36-37	22.00957515621422	27.141641686554674	29.176635645666334	21.67214751156478
38-39	21.689984267909093	27.214269449822844	29.371522570289315	21.724223711978752
40-41	22.014132315609388	27.053796561999377	29.223298657970474	21.708772464420758
42-43	21.999876938453777	27.096330667267743	29.09633084587667	21.807461548401815
44-45	21.961649211262525	27.32506046054822	28.913249429925443	21.800040898263816
46-47	22.09976759860785	27.19260836093505	28.57003753411319	22.13758650634391
48-49	22.003923460656154	27.26225284928813	28.640889560499584	22.09293412955613
50-51	21.941519145715162	27.432351757577777	28.610876575239054	22.01525252146801
52-53	22.073503560504058	27.415406759498584	28.605948433202233	21.905141246795125
54-55	22.049163363828225	27.344462283875824	28.485066486663786	22.121307865632165
56-57	22.187845242005313	27.553195261413517	28.26957242244185	21.989387074139323
58-59	21.951177808569668	27.713912634438024	28.541460588411528	21.79344896858078
60-61	22.11650935513953	27.88153778602171	28.260354572966513	21.741598285872243
62-63	21.901541154823143	28.025726756792444	28.258132412863112	21.814599675521297
64-65	21.897640365894055	27.95772112491185	28.267564223457892	21.8770742857362
66-67	21.969971157865352	27.918695263648974	28.192606607435692	21.91872697104998
68-69	21.94208211672887	27.805164587148486	28.331486845260006	21.92126645086264
70-71	21.98669208543029	27.41794839846522	28.36308513923274	22.23227437687175
72-73	22.101854722245324	27.317915318779267	28.453951099195624	22.126278859779784
74-75	21.98730614819148	27.55114801547876	28.379711217829755	22.0818346185
76-77	22.107731448723236	27.48052239042903	28.21208415629651	22.19966200455123
78-79	22.082258471839683	27.492675710530534	28.20087055111377	22.224195266516013
80-81	22.143444980055587	27.493885346280223	28.18845072521584	22.174218948448356
82-83	22.172096398648637	27.54125563733724	28.23602946966283	22.050618494351287
84-85	22.33337496191613	27.470139524202065	28.11970257076056	22.07678294312125
86-87	22.10880162656096	27.513513285025432	28.213414266858717	22.16427082155489
88-89	22.250014134795816	27.549719811009783	28.084164441547554	22.116101612646855
90-91	22.223865766684224	27.55548928707674	28.144482712815094	22.07616223342394
92-93	22.23858567530041	27.590098656277924	28.07999018845757	22.09132547996409
94-95	22.131445432192002	27.68950753020213	28.06635027417591	22.112696763429955
96-97	22.239474727993947	27.750807480901834	28.136103106600874	21.873614684503345
98-99	23.251704933960298	29.700654338226634	28.39532981750174	18.65231091031133
100	22.238472025789026	30.541008880904148	22.321700240816483	24.898818852490347
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1256.0
1	1117.0
2	1301.0
3	1739.0
4	2222.5
5	3071.0
6	3895.5
7	5410.0
8	7015.5
9	7583.0
10	7479.0
11	7409.5
12	8128.0
13	10039.5
14	14093.5
15	18887.0
16	22181.5
17	23590.5
18	23089.0
19	21379.0
20	19228.0
21	17602.0
22	17711.0
23	20340.5
24	25511.0
25	32809.5
26	43223.0
27	55112.5
28	66206.5
29	78470.5
30	90909.0
31	103617.5
32	117528.0
33	130598.5
34	144173.0
35	157573.5
36	171591.5
37	184133.5
38	192268.0
39	199799.0
40	209614.0
41	217572.0
42	223176.5
43	233116.5
44	242458.0
45	246439.0
46	244878.5
47	240238.0
48	235875.5
49	230018.5
50	223257.0
51	215443.5
52	205521.5
53	194015.0
54	181151.5
55	167524.0
56	153357.5
57	137903.0
58	121928.0
59	105661.5
60	88416.0
61	71332.5
62	55662.5
63	42342.0
64	31729.5
65	23364.5
66	17065.0
67	12864.5
68	9733.5
69	7052.5
70	5050.0
71	3620.0
72	2581.0
73	1703.0
74	1100.5
75	725.0
76	458.5
77	269.5
78	142.5
79	84.5
80	55.5
81	37.5
82	25.5
83	20.0
84	13.0
85	7.5
86	5.0
87	4.0
88	3.5
89	2.5
90	2.0
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007774809302664395
3	2.1012998115309178E-4
4	8.755415881378824E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.75116597008203E-5
24-25	7.880707799641585E-5
26-27	5.954822513012162E-4
28-29	0.0013576703777783518
30-31	5.959982747602881E-4
32-33	7.284164927535723E-4
34-35	0.0010198275577099315
36-37	0.0
38-39	1.7699345292367962E-4
40-41	9.422403132362364E-4
42-43	3.840077044448088E-4
44-45	0.0
46-47	0.0
48-49	0.0016112328234160738
50-51	0.0034625731113610233
52-53	0.0
54-55	0.003892537421881639
56-57	0.03609741264566053
58-59	0.0529209182511721
60-61	0.027984719208796353
62-63	0.006873350146068211
64-65	0.0
66-67	0.0
68-69	4.8771452001234114E-5
70-71	0.0029351211175759288
72-73	0.007769356167083138
74-75	0.0056356891951503795
76-77	0.006430276491778649
78-79	0.009392893730268932
80-81	0.009972241355409757
82-83	0.038670153764743
84-85	0.022970766374107998
86-87	0.0010461788634038075
88-89	0.001536135343764688
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	30.0
20-21	160.0
22-23	344.0
24-25	393.0
26-27	1033.0
28-29	2451.0
30-31	6975.0
32-33	9270.0
34-35	13131.0
36-37	20814.0
38-39	25133.0
40-41	25258.0
42-43	29538.0
44-45	36292.0
46-47	38134.0
48-49	35260.0
50-51	32177.0
52-53	31241.0
54-55	33847.0
56-57	35347.0
58-59	36097.0
60-61	36702.0
62-63	36420.0
64-65	41557.0
66-67	45286.0
68-69	47434.0
70-71	53665.0
72-73	40888.0
74-75	40138.0
76-77	42146.0
78-79	44010.0
80-81	43944.0
82-83	41735.0
84-85	41660.0
86-87	43800.0
88-89	45639.0
90-91	46673.0
92-93	46574.0
94-95	54772.0
96-97	180413.0
98-99	400220.0
100-101	3924150.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.09848389085417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.80012456523949	79.08553186733064
2	6.671056701836804	11.620778493598152
3	1.500576064054816	3.9209370042623926
4	0.48675741175402515	1.6958333034564732
5	0.21965115308446442	0.9565641209267383
6	0.11760051348919835	0.6145695857817072
7	0.06044996140364219	0.368556999266252
8	0.03955538600439375	0.27561713205601657
9	0.023271733965745573	0.1824239471334961
>10	0.07879668127331761	1.0228818731009215
>50	0.0014672096267150413	0.08905537199120885
>100	6.334924615946904E-4	0.11611310446389828
>500	2.9562902887126188E-5	0.020573323326841156
>1k	2.9562902865304285E-5	0.030563873305299624
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.5021663525515296E-5	0.0	0.0	0.0
3	0.0	3.5021663525515296E-5	0.0	0.0	0.0
4	0.0	3.5021663525515296E-5	0.0	0.0	0.0
5	0.0	5.2532495288272944E-5	0.0	0.0	0.0
6	0.0	5.2532495288272944E-5	0.0	0.0	0.0
7	0.0	5.2532495288272944E-5	0.0	0.0	0.0
8	0.0	5.2532495288272944E-5	0.0	0.0	0.0
9	0.0	1.0506499057654589E-4	0.0	3.5021663525515296E-5	0.0
10-11	0.0	1.0506499057654589E-4	0.0	3.5021663525515296E-5	0.0
12-13	0.0	1.0506499057654589E-4	0.0	3.5021663525515296E-5	0.0
14-15	0.0	2.4515164467860703E-4	0.0	3.5021663525515296E-5	8.755415881378824E-6
16-17	0.0	2.626624764413647E-4	0.0	3.5021663525515296E-5	1.7510831762757648E-5
18-19	0.0	2.626624764413647E-4	0.0	5.2532495288272944E-5	4.377707940689412E-5
20-21	0.0	2.626624764413647E-4	0.0	5.2532495288272944E-5	8.755415881378823E-5
22-23	0.0	3.327058034923953E-4	0.0	5.2532495288272944E-5	1.0506499057654589E-4
24-25	0.0	3.589720511365318E-4	0.0	7.004332705103059E-5	1.2257582233930354E-4
26-27	0.0	3.677274670179106E-4	0.0	7.879874293240941E-5	1.2257582233930354E-4
28-29	0.0	3.677274670179106E-4	0.0	2.1888539703447058E-4	1.2257582233930354E-4
30-31	0.0	4.5528162583169884E-4	0.0	8.580307563751247E-4	1.3133123822068238E-4
32-33	0.0	4.5528162583169884E-4	0.0	0.002048767316242645	1.4008665410206118E-4
34-35	0.0	5.078141211199718E-4	0.0	0.003537188016077045	1.5759748586481883E-4
36-37	0.0	5.691020322896236E-4	0.0	0.005542178252912795	1.5759748586481883E-4
38-39	0.0	5.778574481710024E-4	0.0	0.008781682129022961	1.5759748586481883E-4
40-41	0.0	6.479007752220329E-4	0.0	0.012248826818048975	1.5759748586481883E-4
42-43	0.0	6.566561911034118E-4	0.0	0.015672194427668096	1.5759748586481883E-4
44-45	0.0	7.617211816799577E-4	0.0	0.01969968573310235	1.5759748586481883E-4
46-47	0.0	8.755415881378824E-4	0.0	0.024471387388453815	1.7510831762757647E-4
48-49	0.0	8.755415881378824E-4	0.0	0.02879656283385495	1.9261914939033414E-4
50-51	0.0	9.981174104771858E-4	0.0	0.03333186826040918	1.9261914939033414E-4
52-53	0.0	0.0010156282422399436	0.0	0.03761326662640343	1.9261914939033414E-4
54-55	0.0	0.0010331390740027013	0.0	0.04222737079589007	1.9261914939033414E-4
56-57	0.0	0.0010681607375282165	0.0	0.04675392080656292	2.0137456527171294E-4
58-59	0.0	0.0010681607375282165	0.0	0.05184957284952539	2.1012998115309178E-4
60-61	0.0	0.0010681607375282165	0.0	0.05746179442948922	2.1012998115309178E-4
62-63	1.7510831762757648E-5	0.0010856715692909742	0.0	0.06186576861782277	2.2764081291584942E-4
64-65	1.7510831762757648E-5	0.0012257582233930354	0.0	0.06678631234315767	2.3639622879722825E-4
66-67	3.5021663525515296E-5	0.0012257582233930354	0.0	0.07205707270374773	2.451516446786071E-4
68-69	3.5021663525515296E-5	0.0012695353027999295	0.0	0.07666242145735298	2.451516446786071E-4
70-71	3.5021663525515296E-5	0.0012958015504440658	0.0	0.08067240193102448	2.451516446786071E-4
72-73	3.5021663525515296E-5	0.0012958015504440658	0.0	0.08385937331184637	2.626624764413647E-4
74-75	3.5021663525515296E-5	0.0013133123822068236	0.0	0.08645973182861588	2.626624764413647E-4
76-77	3.5021663525515296E-5	0.0013658448774950965	0.0	0.08917391075184333	2.626624764413647E-4
78-79	3.5021663525515296E-5	0.0014008665410206117	0.0	0.09110885766162805	2.626624764413647E-4
80-81	3.5021663525515296E-5	0.0014008665410206117	0.0	0.09185306801154525	2.714178923227435E-4
82-83	3.5021663525515296E-5	0.0014008665410206117	0.0	0.09229959422149556	2.9768413996688004E-4
84-85	3.5021663525515296E-5	0.0014008665410206117	0.0	0.09254474586617417	2.9768413996688004E-4
86-87	3.5021663525515296E-5	0.0014183773727833695	0.0	0.09259727836146245	2.9768413996688004E-4
88	3.5021663525515296E-5	0.0014884206998344001	0.0	0.09259727836146245	3.1519497172963765E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6940	0.0	49.904022	1
GTATCAA	12865	0.0	38.03341	1
ATCAACG	15405	0.0	32.240532	3
TCAACGC	15515	0.0	32.011948	4
CAACGCA	15890	0.0	31.256477	5
TATCAAC	16345	0.0	30.653572	2
AACGCAG	16415	0.0	30.283272	6
CTTATAC	4565	0.0	29.794783	1
ACGCAGA	18970	0.0	26.158718	7
CGCAGAG	19180	0.0	25.894962	8
TATACAC	6955	0.0	23.491371	3
TGGTATC	3295	0.0	22.155958	2
GTGGTAT	3390	0.0	21.79144	1
GCAGAGT	22880	0.0	21.612446	9
TTATACA	7470	0.0	20.88385	2
GAGTACT	14275	0.0	20.501194	12-13
TACATGG	15180	0.0	20.23878	2
GTACATG	15390	0.0	19.623789	1
ACATGGG	15545	0.0	19.427267	3
ACATCTC	7535	0.0	19.31876	8
>>END_MODULE