FastQCFastQC Report
Fri 27 May 2016
SRR2140130_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140130_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5591602
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79200.044.449921
GTATCAA139900.035.4386831
CTTATAC44700.034.275621
TCAACGC167450.030.144034
ATCAACG167700.030.1247523
CAACGCA170600.029.5869145
AACGCAG176200.028.7198456
TATCAAC179350.028.4114342
TATACAC70300.025.463993
ACGCAGA202950.024.91327
CGCAGAG204700.024.7002148
TTATACA72250.023.052392
GCAGAGT241450.020.8694219
GTGGTAT42350.020.2228571
GAGTACT146450.019.83330512-13
TGGTATC41450.019.8313242
ACACATC83400.019.3484386
AGAGTAC213200.019.29529410-11
ACATCTC84950.018.9954058
TACATGG152300.018.6220862
Produced by FastQC (version 0.11.5)