##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140130_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5591602
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.685682743514292	18.0	18.0	30.0	18.0	33.0
2	29.978823600106015	31.0	27.0	33.0	27.0	33.0
3	31.256930482534344	33.0	31.0	33.0	29.0	33.0
4	32.415531362926046	33.0	33.0	33.0	31.0	34.0
5	33.0204066741517	33.0	33.0	34.0	33.0	34.0
6	36.99714625611766	38.0	37.0	38.0	35.0	38.0
7	37.125544343105965	38.0	38.0	38.0	36.0	38.0
8	37.092738538973265	38.0	38.0	38.0	36.0	38.0
9	37.33973984557556	38.0	38.0	38.0	37.0	38.0
10-11	37.36072005840187	38.0	38.0	38.0	37.0	38.0
12-13	37.41712884786864	38.0	38.0	38.0	37.0	38.0
14-15	37.37877579627448	38.0	38.0	38.0	37.0	38.0
16-17	37.39175141578389	38.0	38.0	38.0	37.0	38.0
18-19	37.438538722891934	38.0	38.0	38.0	37.0	38.0
20-21	37.45521055399363	38.0	38.0	38.0	37.0	38.0
22-23	37.479501578647906	38.0	38.0	38.0	37.0	38.0
24-25	37.49151559082813	38.0	38.0	38.0	37.0	38.0
26-27	37.46061675577796	38.0	38.0	38.0	37.0	38.0
28-29	37.436505434738166	38.0	38.0	38.0	37.0	38.0
30-31	37.43694078420792	38.0	38.0	38.0	37.0	38.0
32-33	37.42294036434289	38.0	38.0	38.0	37.0	38.0
34-35	37.392689050961074	38.0	38.0	38.0	37.0	38.0
36-37	37.3647304388899	38.0	38.0	38.0	37.0	38.0
38-39	37.33754115390926	38.0	38.0	38.0	37.0	38.0
40-41	37.34349216073838	38.0	38.0	38.0	37.0	38.0
42-43	37.3108767985876	38.0	38.0	38.0	37.0	38.0
44-45	37.26564608451583	38.0	38.0	38.0	37.0	38.0
46-47	37.22842638072305	38.0	38.0	38.0	37.0	38.0
48-49	37.1785042485862	38.0	38.0	38.0	37.0	38.0
50-51	37.13327757990283	38.0	38.0	38.0	36.5	38.0
52-53	37.088925675956716	38.0	38.0	38.0	36.0	38.0
54-55	37.06494204649718	38.0	38.0	38.0	36.0	38.0
56-57	37.018830598433524	38.0	38.0	38.0	36.0	38.0
58-59	36.982639810505205	38.0	38.0	38.0	36.0	38.0
60-61	37.00012853201582	38.0	38.0	38.0	36.0	38.0
62-63	37.00920463294272	38.0	38.0	38.0	36.0	38.0
64-65	37.01426267053901	38.0	38.0	38.0	36.0	38.0
66-67	37.003996546346656	38.0	38.0	38.0	36.0	38.0
68-69	36.963666451686734	38.0	38.0	38.0	36.0	38.0
70-71	36.958111476999704	38.0	38.0	38.0	36.0	38.0
72-73	36.967152126051545	38.0	38.0	38.0	36.0	38.0
74-75	36.97827867132405	38.0	38.0	38.0	36.0	38.0
76-77	36.95708876788561	38.0	38.0	38.0	36.0	38.0
78-79	36.91445090474347	38.0	38.0	38.0	35.0	38.0
80-81	36.86563399320212	38.0	38.0	38.0	35.0	38.0
82-83	36.820816354389535	38.0	38.0	38.0	35.0	38.0
84-85	36.78692682734938	38.0	38.0	38.0	35.0	38.0
86-87	36.80746095820969	38.0	38.0	38.0	35.0	38.0
88-89	36.761646922654094	38.0	38.0	38.0	35.0	38.0
90-91	36.769624925933535	38.0	38.0	38.0	35.0	38.0
92-93	36.75882148606013	38.0	38.0	38.0	35.0	38.0
94-95	36.76816868634636	38.0	38.0	38.0	35.0	38.0
96-97	36.76355014436359	38.0	38.0	38.0	35.0	38.0
98-99	36.740061332484856	38.0	38.0	38.0	34.0	38.0
100	35.0649156006318	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	10.0
20	23.0
21	78.0
22	348.0
23	927.0
24	2349.0
25	4819.0
26	8519.0
27	14646.0
28	23239.0
29	34295.0
30	46214.0
31	60304.0
32	78147.0
33	105624.0
34	160344.0
35	296213.0
36	951797.0
37	3803700.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.1605836753045	19.66177134924839	17.182034772861158	22.99561020258595
2	14.375408677337706	20.930056617460842	42.19567578240695	22.4988589227945
3	18.148542589368507	25.7889564605448	30.995395768112676	25.067105181974014
4	12.497008885889132	18.210725375127737	38.53282857276732	30.759437166215807
5	13.382193510911542	37.21829629505104	35.76769233575637	13.631817858281043
6	29.89874815839897	37.75996574863519	18.731054177318057	13.61023191564779
7	26.390469135678828	32.628162734042945	22.859960347678538	18.12140778259969
8	24.093721262707895	37.01965912452281	21.165490676911556	17.721128935857738
9	25.13914616955928	17.288784144508142	21.34846149636544	36.223608189567145
10-11	24.113474099193756	26.82274239117877	28.841412532580108	20.222370977047365
12-13	24.98132020125896	24.23885855967574	29.05084267442497	21.72897856464033
14-15	22.035652752109325	25.696329960537252	26.748202035838748	25.519815251514682
16-17	21.620324550996298	28.96290723123713	27.39819464976227	22.018573568004303
18-19	21.619573424574927	27.81699233958354	29.368095225661627	21.195339010179907
20-21	22.320328933196375	26.93882292155967	29.347957594079116	21.392890551164836
22-23	22.021248873912345	27.01368260218938	29.252676632337497	21.712391891560774
24-25	21.881698976170625	27.109567682900327	29.374142463976554	21.63459087695249
26-27	21.809784852034063	27.242162543619685	29.327285273528915	21.620767330817333
28-29	21.57031451650061	27.140739971987905	29.507426425756094	21.781519085755388
30-31	21.964884892542376	26.953895417154182	29.228951403132346	21.85226828717109
32-33	21.89105901485639	27.080751546202798	29.378041427438497	21.65014801150232
34-35	21.95427190353609	27.30476415281252	29.28189300614895	21.459070937502442
36-37	21.797997190139316	27.187907720916016	29.21925241266233	21.794842676282347
38-39	21.688676017294316	27.188715358780318	29.394554902922504	21.72805372100286
40-41	21.966482511140565	27.02825219705688	29.29387936561675	21.711385926185805
42-43	21.97787568961774	27.08167139945179	29.149643995807057	21.790808915123414
44-45	21.910496081925526	27.31110587839635	29.01428699958827	21.76411104008985
46-47	22.113465200268735	27.179098632953867	28.625159142283408	22.082277024493994
48-49	22.003941488982225	27.27939737500237	28.631223186509736	22.085437949505668
50-51	21.89560735778048	27.446346182187327	28.66197988838179	21.996066571650406
52-53	22.071001845291967	27.42516368577639	28.69556966896592	21.808264799965727
54-55	21.99286462589	27.36992980562122	28.53886282682686	22.09834274166192
56-57	22.153046380983422	27.62530249407991	28.314561154039552	21.90708997089711
58-59	21.900656774677106	27.81452577705935	28.544130088434184	21.740687359829355
60-61	22.07141343411895	28.02644080099419	28.251980439109715	21.650165325777145
62-63	21.87679164965486	28.175283912384007	28.235006797019008	21.712917640942123
64-65	21.867199300184907	28.08496797338736	28.28646023548968	21.76137249093805
66-67	21.946122692022605	27.976909296397224	28.25371801965863	21.823249991921536
68-69	21.927085317614107	27.887441168864623	28.344417346783374	21.841056166737893
70-71	21.941347415946108	27.492045332665977	28.407309611485367	22.159297639902555
72-73	22.046513971621422	27.370822288178786	28.556398644925785	22.02626509527401
74-75	21.986442774320736	27.55611776396072	28.42547405350466	22.031965408213882
76-77	22.08463331054137	27.50289943385776	28.28424655123153	22.12822070436934
78-79	22.032176179417892	27.54653475996755	28.256266294870198	22.165022765744357
80-81	22.09481870751815	27.555696711675353	28.24225103782776	22.107233542978737
82-83	22.10134881266902	27.56842321973918	28.30867608817172	22.021551879420084
84-85	22.245493063690464	27.515974746090787	28.204878506774733	22.033653683444015
86-87	22.073594514540176	27.550989040120555	28.272249174491492	22.103167270847774
88-89	22.187849243528017	27.537307262640702	28.185810381858694	22.089033111972586
90-91	22.200311896984264	27.576987269435122	28.16889213826871	22.053808695311908
92-93	22.18897897266617	27.60661102557256	28.15438983233812	22.050020169423153
94-95	22.146805289648615	27.69106215347461	28.11601038466235	22.046122172214424
96-97	22.222892449050835	27.76668857024983	28.17238711098583	21.838031869713507
98-99	23.25338966965262	29.703561292592852	28.40918629527111	18.633862742483416
100	22.158894825443703	30.573141810818882	22.359696593458633	24.908266770278782
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1388.0
1	1285.0
2	1441.5
3	1807.0
4	2299.0
5	3218.0
6	4090.5
7	5605.5
8	7370.5
9	7930.0
10	7585.0
11	7358.0
12	8041.0
13	10029.0
14	14155.0
15	19057.5
16	22543.5
17	24118.5
18	23564.0
19	21711.5
20	19620.5
21	18011.5
22	17958.5
23	20394.5
24	25484.0
25	32756.5
26	42401.0
27	53733.5
28	65023.0
29	77621.5
30	89986.0
31	102226.0
32	116628.0
33	130073.5
34	142862.0
35	155240.5
36	168210.0
37	181090.0
38	190494.0
39	198205.0
40	207553.5
41	215998.0
42	220617.0
43	225549.0
44	234091.0
45	246640.5
46	249480.0
47	244646.0
48	240244.5
49	231583.5
50	223619.0
51	214685.5
52	203285.0
53	191020.5
54	177851.5
55	163955.0
56	149153.5
57	133466.5
58	117844.0
59	102295.5
60	85647.0
61	69470.0
62	54977.0
63	42152.0
64	31740.5
65	23413.0
66	17065.5
67	12926.0
68	9672.5
69	7012.0
70	5114.0
71	3675.5
72	2642.0
73	1843.5
74	1245.0
75	813.5
76	517.0
77	314.0
78	199.0
79	124.0
80	76.5
81	51.0
82	37.0
83	26.5
84	15.5
85	9.0
86	7.5
87	5.5
88	3.5
89	2.5
90	1.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007046281190971747
3	1.251877368954371E-4
4	7.15358496545355E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	7.154512610454496E-5
26-27	6.70809586673965E-4
28-29	0.0013598787632296298
30-31	7.699915318838274E-4
32-33	7.622005213272225E-4
34-35	0.0010153349724853207
36-37	0.0
38-39	2.263601665503779E-4
40-41	8.736731430418624E-4
42-43	3.476725382153877E-4
44-45	0.0
46-47	0.0
48-49	0.0018139085081378575
50-51	0.003606532739689883
52-53	0.0
54-55	0.0036258576703921716
56-57	0.03633895896811581
58-59	0.053026951945728915
60-61	0.02800768093571808
62-63	0.006392851336602604
64-65	0.0
66-67	0.0
68-69	6.063368874443699E-5
70-71	0.0033809385138027586
72-73	0.007299801397906805
74-75	0.005259144611063866
76-77	0.006672166913869575
78-79	0.009631324972254253
80-81	0.010667693735653907
82-83	0.03968797680318862
84-85	0.023564906473298366
86-87	0.001167911906828836
88-89	0.0016135256626249148
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	42.0
20-21	205.0
22-23	382.0
24-25	504.0
26-27	1153.0
28-29	2942.0
30-31	8131.0
32-33	10328.0
34-35	14810.0
36-37	23851.0
38-39	28342.0
40-41	28244.0
42-43	33294.0
44-45	40543.0
46-47	41250.0
48-49	38748.0
50-51	35390.0
52-53	34263.0
54-55	37222.0
56-57	38731.0
58-59	39304.0
60-61	40283.0
62-63	39656.0
64-65	44434.0
66-67	49242.0
68-69	50146.0
70-71	56759.0
72-73	42078.0
74-75	41977.0
76-77	44217.0
78-79	45334.0
80-81	45373.0
82-83	42931.0
84-85	42615.0
86-87	44136.0
88-89	46268.0
90-91	46614.0
92-93	46523.0
94-95	54865.0
96-97	173602.0
98-99	384413.0
100-101	3752457.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.74554978174349
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.16886936884194	79.9966256574899
2	6.500695314580722	11.408141686829788
3	1.3743300768126212	3.617740445145276
4	0.46876380197583567	1.6452775008860012
5	0.2012571021460762	0.882970753764398
6	0.10494693859637474	0.5525176095029868
7	0.05899701998569438	0.36237081678904837
8	0.03341265345618488	0.23454493177438515
9	0.020888470710513463	0.1649583311934465
>10	0.06572137638010313	0.8832573994306963
>50	0.0011852596079794295	0.06904308884091336
>100	8.737925291178353E-4	0.1471949761615205
>500	5.8824376844150765E-5	0.03535680219161369
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.365188724090162E-5	0.0	0.0	0.0
3	0.0	7.15358496545355E-5	0.0	0.0	0.0
4	0.0	1.0730377448180325E-4	0.0	0.0	0.0
5	0.0	1.0730377448180325E-4	0.0	1.7883962413633876E-5	0.0
6	7.15358496545355E-5	1.0730377448180325E-4	0.0	1.7883962413633876E-5	1.7883962413633876E-5
7	7.15358496545355E-5	1.0730377448180325E-4	0.0	1.7883962413633876E-5	1.7883962413633876E-5
8	7.15358496545355E-5	1.0730377448180325E-4	0.0	1.7883962413633876E-5	1.7883962413633876E-5
9	7.15358496545355E-5	1.6095566172270488E-4	0.0	3.576792482726775E-5	1.7883962413633876E-5
10-11	7.15358496545355E-5	1.6095566172270488E-4	0.0	3.576792482726775E-5	1.7883962413633876E-5
12-13	8.047783086135244E-5	1.6095566172270488E-4	0.0	3.576792482726775E-5	1.7883962413633876E-5
14-15	8.941981206816937E-5	2.2354953017042343E-4	0.0	4.470990603408469E-5	3.576792482726775E-5
16-17	1.2518773689543713E-4	2.414334925840573E-4	0.0	5.365188724090162E-5	5.365188724090162E-5
18-19	1.43071699309071E-4	2.86143398618142E-4	0.0	5.365188724090162E-5	5.365188724090162E-5
20-21	1.43071699309071E-4	3.040273610317759E-4	0.0	6.259386844771857E-5	5.365188724090162E-5
22-23	1.43071699309071E-4	3.397952858590436E-4	0.0	8.047783086135244E-5	6.259386844771857E-5
24-25	1.43071699309071E-4	3.6662122947949446E-4	0.0	1.0730377448180325E-4	7.15358496545355E-5
26-27	1.43071699309071E-4	3.7556321068631137E-4	0.0	1.43071699309071E-4	7.15358496545355E-5
28-29	1.43071699309071E-4	3.9344717309994524E-4	0.0	3.3085330465222666E-4	7.15358496545355E-5
30-31	1.43071699309071E-4	5.007509475817485E-4	0.0	8.67372177061243E-4	7.15358496545355E-5
32-33	1.43071699309071E-4	5.007509475817485E-4	0.0	0.0023606830385996718	7.15358496545355E-5
34-35	1.6095566172270488E-4	6.348806656840025E-4	0.0	0.004104369373928975	7.15358496545355E-5
36-37	1.698976429295218E-4	7.779523649930735E-4	0.0	0.0066170660930445335	7.15358496545355E-5
38-39	1.7883962413633875E-4	7.958363274067074E-4	0.0	0.01022068451939176	7.15358496545355E-5
40-41	1.7883962413633875E-4	8.941981206816937E-4	0.0	0.014486009555043438	7.15358496545355E-5
42-43	1.7883962413633875E-4	9.031401018885106E-4	0.0	0.018903348271211005	8.047783086135244E-5
44-45	1.8778160534315568E-4	0.001010443876370314	0.0	0.02306136953238088	8.941981206816937E-5
46-47	1.9672358654997262E-4	0.0011088056696453002	0.0	0.027898981365268846	8.941981206816937E-5
48-49	1.9672358654997262E-4	0.001126689632058934	0.0	0.03295120074712041	1.0730377448180325E-4
50-51	2.146075489636065E-4	0.001296587274988456	0.0	0.03834321541483103	1.0730377448180325E-4
52-53	2.146075489636065E-4	0.0013055292561952728	0.0	0.04373523008254164	1.0730377448180325E-4
54-55	2.146075489636065E-4	0.0013055292561952728	0.0	0.04941338814887039	1.0730377448180325E-4
56-57	2.146075489636065E-4	0.0013055292561952728	0.0	0.0555922971627809	1.0730377448180325E-4
58-59	2.146075489636065E-4	0.0013144712374020897	0.0	0.06165496042100278	1.162457556886202E-4
60-61	2.146075489636065E-4	0.0013681231246429914	0.0	0.06700226518267932	1.2518773689543713E-4
62-63	2.5037547379087426E-4	0.001412833030677076	0.0	0.07302021853486712	1.2518773689543713E-4
64-65	2.5037547379087426E-4	0.0016453245420543165	0.0	0.07843011716499135	1.2518773689543713E-4
66-67	2.5037547379087426E-4	0.0016632085044679503	0.0	0.08403673938166559	1.3412971810225407E-4
68-69	2.5037547379087426E-4	0.0016989764292952181	0.0	0.08951817386144437	1.43071699309071E-4
70-71	2.7720141741132505E-4	0.0017258023729156689	0.0	0.09436472767553913	1.6095566172270488E-4
72-73	3.040273610317759E-4	0.0017347443541224858	0.0	0.09791469421464546	1.7883962413633875E-4
74-75	3.040273610317759E-4	0.0017436863353293029	0.0	0.10105332961823821	1.7883962413633875E-4
76-77	3.040273610317759E-4	0.0018062802037770213	0.0	0.10387005369838555	1.7883962413633875E-4
78-79	3.040273610317759E-4	0.0018241641661906553	0.0	0.10583728956388527	1.7883962413633875E-4
80-81	3.040273610317759E-4	0.0018331061473974722	0.0	0.10632015654905339	1.8778160534315568E-4
82-83	3.040273610317759E-4	0.001842048128604289	0.0	0.10684773344025558	1.9672358654997262E-4
84-85	3.040273610317759E-4	0.001850990109811106	0.0	0.10712493485766691	1.9672358654997262E-4
86-87	3.040273610317759E-4	0.0018957000158451908	0.0	0.10724118061335552	1.9672358654997262E-4
88	3.040273610317759E-4	0.00198511982791336	0.0	0.10726800655697598	1.9672358654997262E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7920	0.0	44.44992	1
GTATCAA	13990	0.0	35.438683	1
CTTATAC	4470	0.0	34.27562	1
TCAACGC	16745	0.0	30.14403	4
ATCAACG	16770	0.0	30.124752	3
CAACGCA	17060	0.0	29.586914	5
AACGCAG	17620	0.0	28.719845	6
TATCAAC	17935	0.0	28.411434	2
TATACAC	7030	0.0	25.46399	3
ACGCAGA	20295	0.0	24.9132	7
CGCAGAG	20470	0.0	24.700214	8
TTATACA	7225	0.0	23.05239	2
GCAGAGT	24145	0.0	20.869421	9
GTGGTAT	4235	0.0	20.222857	1
GAGTACT	14645	0.0	19.833305	12-13
TGGTATC	4145	0.0	19.831324	2
ACACATC	8340	0.0	19.348438	6
AGAGTAC	21320	0.0	19.295294	10-11
ACATCTC	8495	0.0	18.995405	8
TACATGG	15230	0.0	18.622086	2
>>END_MODULE