FastQCFastQC Report
Fri 27 May 2016
SRR2140129_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140129_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5143810
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69450.049.7265551
GTATCAA125200.038.0060841
ATCAACG152600.031.3796123
TCAACGC153450.031.2057934
CAACGCA156750.030.5207885
TATCAAC162250.029.8366872
AACGCAG161150.029.7144436
ACGCAGA185800.025.795657
CGCAGAG188000.025.401258
GTGGTAT34350.023.6785471
TGGTATC34150.023.3077582
GCAGAGT218900.021.8354689
GAGTACT139100.020.35468712-13
CAGAGTA215800.019.38805410-11
GTACATG141750.019.147041
AGAGTAC197700.019.12817410-11
TACATGG138650.019.1045822
ACATGGG141950.018.138493
GTACTTT156050.017.96279514-15
AGTACTT151300.017.24737212-13
Produced by FastQC (version 0.11.5)