##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140129_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5143810
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.877936393451545	18.0	18.0	30.0	18.0	33.0
2	30.08641590571969	31.0	28.0	33.0	27.0	33.0
3	31.318846341525056	33.0	31.0	33.0	29.0	33.0
4	32.44400279170498	33.0	33.0	33.0	31.0	34.0
5	33.044563076785494	33.0	33.0	34.0	33.0	34.0
6	37.017425604756006	38.0	38.0	38.0	35.0	38.0
7	37.165237635138155	38.0	38.0	38.0	36.0	38.0
8	37.13254727526872	38.0	38.0	38.0	36.0	38.0
9	37.36792124903525	38.0	38.0	38.0	37.0	38.0
10-11	37.383942155717264	38.0	38.0	38.0	37.0	38.0
12-13	37.44069979256621	38.0	38.0	38.0	37.0	38.0
14-15	37.40407625864874	38.0	38.0	38.0	37.0	38.0
16-17	37.42078439911272	38.0	38.0	38.0	37.0	38.0
18-19	37.46354113001841	38.0	38.0	38.0	37.0	38.0
20-21	37.47868541449816	38.0	38.0	38.0	37.0	38.0
22-23	37.50104257045776	38.0	38.0	38.0	37.0	38.0
24-25	37.51233208190285	38.0	38.0	38.0	37.5	38.0
26-27	37.483048922009296	38.0	38.0	38.0	37.0	38.0
28-29	37.45974528788901	38.0	38.0	38.0	37.0	38.0
30-31	37.459553520021416	38.0	38.0	38.0	37.0	38.0
32-33	37.44877921453936	38.0	38.0	38.0	37.0	38.0
34-35	37.417839696677646	38.0	38.0	38.0	37.0	38.0
36-37	37.390637903923945	38.0	38.0	38.0	37.0	38.0
38-39	37.37492864515936	38.0	38.0	38.0	37.0	38.0
40-41	37.361559736987424	38.0	38.0	38.0	37.0	38.0
42-43	37.32736174631181	38.0	38.0	38.0	37.0	38.0
44-45	37.2815355523649	38.0	38.0	38.0	37.0	38.0
46-47	37.241146487419044	38.0	38.0	38.0	37.0	38.0
48-49	37.189630765864685	38.0	38.0	38.0	37.0	38.0
50-51	37.1452623600404	38.0	38.0	38.0	37.0	38.0
52-53	37.10609301028379	38.0	38.0	38.0	36.0	38.0
54-55	37.08644788537215	38.0	38.0	38.0	36.0	38.0
56-57	37.0421113043748	38.0	38.0	38.0	36.0	38.0
58-59	37.01412279351053	38.0	38.0	38.0	36.0	38.0
60-61	37.03479737607795	38.0	38.0	38.0	36.0	38.0
62-63	37.04828997854479	38.0	38.0	38.0	36.0	38.0
64-65	37.05257753340756	38.0	38.0	38.0	36.0	38.0
66-67	37.04730152963728	38.0	38.0	38.0	36.0	38.0
68-69	37.01109747761645	38.0	38.0	38.0	36.0	38.0
70-71	37.007313439170574	38.0	38.0	38.0	36.0	38.0
72-73	37.01747602820438	38.0	38.0	38.0	36.0	38.0
74-75	37.03148120535269	38.0	38.0	38.0	36.0	38.0
76-77	37.0099369110315	38.0	38.0	38.0	36.0	38.0
78-79	36.96947690168332	38.0	38.0	38.0	36.0	38.0
80-81	36.92333213110203	38.0	38.0	38.0	36.0	38.0
82-83	36.88344892771705	38.0	38.0	38.0	35.0	38.0
84-85	36.854491806737016	38.0	38.0	38.0	35.0	38.0
86-87	36.87444804725412	38.0	38.0	38.0	35.0	38.0
88-89	36.82975461024681	38.0	38.0	38.0	35.0	38.0
90-91	36.83907009999582	38.0	38.0	38.0	35.0	38.0
92-93	36.826135318415325	38.0	38.0	38.0	35.0	38.0
94-95	36.83772542921792	38.0	38.0	38.0	35.0	38.0
96-97	36.835628043197744	38.0	38.0	38.0	35.0	38.0
98-99	36.81234972992436	38.0	38.0	38.0	35.0	38.0
100	35.18237701262304	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	11.0
21	74.0
22	235.0
23	763.0
24	1935.0
25	4039.0
26	7402.0
27	12915.0
28	20937.0
29	30952.0
30	41753.0
31	53694.0
32	69178.0
33	93278.0
34	138996.0
35	256939.0
36	815261.0
37	3595444.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.60678757574638	19.58818463356928	16.912522041055173	22.892505749629166
2	14.418449474679452	21.006056074243048	42.19413072713525	22.38136372394225
3	18.138323672845296	25.771272433538982	31.122039035305576	24.968364858310142
4	12.505616018137673	18.013989352943337	39.09160772966282	30.38878689925617
5	13.348840645358207	37.217607182224846	35.879416230381764	13.554135942035185
6	29.767662491421724	37.93464377572266	18.918019911310875	13.37967382154473
7	26.336587082337797	32.72033764855234	23.274421100312804	17.66865416879706
8	24.06850175259195	36.9238171705409	21.31309282419063	17.694588252676517
9	25.33184546085489	17.224508681308212	21.616233881111473	35.82741197672542
10-11	24.040526380251215	26.850855688682124	29.098946111928708	20.009671819137953
12-13	24.92500695010119	24.33512318689843	29.335249552374602	21.404620310625784
14-15	22.03228735120465	25.730625742397173	27.181350010984072	25.0557368954141
16-17	21.484162517666867	28.898938724408563	27.812866338375642	21.804032419548932
18-19	21.454641598348307	27.855441783425128	29.804143232351116	20.88577338587545
20-21	22.247600387888365	27.008846467595227	29.808847556295525	20.934705588220883
22-23	21.934380700423297	26.854783167016056	29.691695347506798	21.519140785053853
24-25	21.725084444064223	26.94169337265388	29.825531685298884	21.507690497983013
26-27	21.65481997695187	27.1770376609327	29.693032855301276	21.475109506814153
28-29	21.609573308136866	27.231534532395653	29.719970700940706	21.438921458526778
30-31	21.957642636174352	27.000024230008755	29.640949220810597	21.40138391300629
32-33	21.64519284158415	27.221567337377216	29.5626775459716	21.57056227506703
34-35	21.799334331689412	27.204188215982576	29.58154436804695	21.414933084281067
36-37	21.7962267650737	27.105606573200134	29.6252445916878	21.47292207003836
38-39	21.57953828642173	27.28040600252789	29.693589222912543	21.446466488137837
40-41	21.90156768966631	27.098644112318947	29.498090429587275	21.501697768427462
42-43	21.801576109678898	27.178378170640254	29.34598708719634	21.67405863248451
44-45	21.868222137247418	27.313551158579692	29.196299804264665	21.621926899908235
46-47	21.938605410783083	27.269834964026945	28.930662792000007	21.860896833189965
48-49	21.84911375354403	27.422759107380923	28.915242332691964	21.812884806383078
50-51	21.846889761652196	27.52240287348313	28.824708916012536	21.805998448852137
52-53	22.03727792263829	27.479040285432525	28.65959159891206	21.824090193017128
54-55	21.985732737355168	27.38516378558145	28.71064898380256	21.918454493260818
56-57	21.994047963949228	27.51591582965944	28.652952964801827	21.837083241589504
58-59	21.91273574780991	27.542675401872103	28.73582991606417	21.80875893425381
60-61	21.995038301493043	27.447053818443624	28.778197476104044	21.77971040395929
62-63	21.924331607125794	27.49782618221635	28.798125580410666	21.77971663024719
64-65	21.871974923230038	27.40014588419432	28.881572231815518	21.846306960760128
66-67	21.956211706889178	27.39423126276973	28.79117937611021	21.858377654230885
68-69	21.900961060657774	27.349143233337514	28.903250720387607	21.8466449856171
70-71	21.879967939251117	27.137316047965566	28.82631457680705	22.156401435976267
72-73	22.01981767980824	27.195410026950956	28.854098554568758	21.930673738672045
74-75	21.873101562663656	27.437450836672955	28.76324382582873	21.926203774834647
76-77	21.993237841699752	27.405670239683182	28.560201433604394	22.040890485012675
78-79	21.99303664310003	27.505034074861445	28.448286270586955	22.05364301145157
80-81	22.025344399813047	27.498310422707007	28.407919831622074	22.068425345857868
82-83	22.051830921761315	27.529349787119507	28.430078981164257	21.988740309954917
84-85	22.20998693049556	27.484792962499537	28.37548299774809	21.92973710925681
86-87	21.984699134037893	27.565956814807425	28.387304282396506	22.06203976875818
88-89	22.075728706196177	27.566226712905863	28.362525432226594	21.99551914867137
90-91	22.117017756436432	27.57340178205115	28.372283624811	21.937296836701417
92-93	22.170892936275173	27.543759565344928	28.283278218583025	22.002069279796878
94-95	22.028101687479023	27.682541512746113	28.24773388066432	22.041622919110544
96-97	22.131462089533795	27.785379836676427	28.324638698175708	21.758519375614068
98-99	23.147092283913928	29.69685947981018	28.597079036534222	18.558969199741675
100	22.061823090508888	30.558937123785785	22.552053732812407	24.82718605289292
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1194.0
1	1135.5
2	1375.5
3	1782.0
4	2222.0
5	3083.0
6	4035.5
7	5705.0
8	7372.0
9	7927.0
10	7688.5
11	7358.5
12	8000.0
13	9944.0
14	13855.5
15	18436.0
16	21510.5
17	22722.5
18	22158.5
19	20374.5
20	18341.0
21	16805.0
22	16954.0
23	19450.5
24	24242.5
25	31695.5
26	41786.5
27	53124.5
28	63226.5
29	73759.5
30	84306.5
31	94743.0
32	107570.5
33	120548.0
34	133187.5
35	145114.5
36	157892.0
37	169431.5
38	176340.5
39	182256.5
40	190161.5
41	197670.0
42	202204.5
43	206808.5
44	210480.5
45	212246.0
46	212885.5
47	211846.0
48	209678.0
49	205502.0
50	199636.5
51	192131.5
52	183216.5
53	173099.0
54	161460.0
55	148410.0
56	134542.0
57	120267.5
58	106160.0
59	92135.0
60	77134.0
61	61774.0
62	47632.5
63	36126.0
64	26902.5
65	19648.5
66	14223.5
67	10606.0
68	8176.0
69	5925.0
70	4154.0
71	2971.0
72	2132.0
73	1433.5
74	893.0
75	558.0
76	346.0
77	203.5
78	125.5
79	82.0
80	53.0
81	29.5
82	18.5
83	17.0
84	15.0
85	11.0
86	6.5
87	4.5
88	3.5
89	2.5
90	1.5
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0073680793030846785
3	9.720421244175038E-5
4	7.97074542022353E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.916472802238451E-5
26-27	4.27787646601125E-4
28-29	0.0010113752486135407
30-31	3.503121524550703E-4
32-33	3.604857711394702E-4
34-35	7.709630714544189E-4
36-37	0.0
38-39	1.571093143042532E-4
40-41	5.521808976173789E-4
42-43	2.2781538712668734E-4
44-45	0.0
46-47	0.0
48-49	0.0011810355966147684
50-51	0.0029657002546439617
52-53	0.0
54-55	0.0032368964267129657
56-57	0.03271700617154655
58-59	0.048031940198776414
60-61	0.025282493325725866
62-63	0.005384759546729946
64-65	0.0
66-67	0.0
68-69	4.321945618903153E-5
70-71	0.0032151267462906426
72-73	0.0059249891970628325
74-75	0.004411135079263553
76-77	0.0059813794626261615
78-79	0.008699207796656526
80-81	0.009738398150820565
82-83	0.03556424111566622
84-85	0.02107203514666937
86-87	9.721657240368209E-4
88-89	0.0011120375461732186
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	36.0
20-21	172.0
22-23	335.0
24-25	386.0
26-27	902.0
28-29	2307.0
30-31	5983.0
32-33	7849.0
34-35	11059.0
36-37	17525.0
38-39	21108.0
40-41	22149.0
42-43	26339.0
44-45	32534.0
46-47	33799.0
48-49	31177.0
50-51	28472.0
52-53	28232.0
54-55	31348.0
56-57	32929.0
58-59	32735.0
60-61	32748.0
62-63	32740.0
64-65	35511.0
66-67	38133.0
68-69	39406.0
70-71	40031.0
72-73	37203.0
74-75	38595.0
76-77	40315.0
78-79	41395.0
80-81	41815.0
82-83	39651.0
84-85	39902.0
86-87	41652.0
88-89	43639.0
90-91	44249.0
92-93	44021.0
94-95	51898.0
96-97	162686.0
98-99	359445.0
100-101	3531399.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.7019313387501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.2603272354924	80.0370695315902
2	6.428313362752975	11.275509943282623
3	1.3564131971591054	3.5688017125266702
4	0.4554674412918098	1.5978149705284197
5	0.1974235606295837	0.8657213779493658
6	0.10845846681779156	0.5707210205976037
7	0.05845322947469147	0.35885227825423166
8	0.03706308769765839	0.2600403497969688
9	0.023997774900615068	0.1894186085954871
>10	0.07191221793090809	0.9519894534735588
>50	0.001033968631630479	0.05842906888802365
>100	0.0010089901154368892	0.17368349508108627
>500	9.560033027545433E-5	0.0571811545870063
>1k	3.186677509040353E-5	0.03476703484881657
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.9440842488350076E-5	0.0	0.0	0.0
3	0.0	1.9440842488350076E-5	0.0	0.0	0.0
4	0.0	3.888168497670015E-5	0.0	0.0	0.0
5	0.0	3.888168497670015E-5	0.0	0.0	0.0
6	7.77633699534003E-5	3.888168497670015E-5	0.0	0.0	1.9440842488350076E-5
7	7.77633699534003E-5	3.888168497670015E-5	0.0	0.0	1.9440842488350076E-5
8	7.77633699534003E-5	3.888168497670015E-5	0.0	0.0	3.888168497670015E-5
9	7.77633699534003E-5	9.720421244175038E-5	0.0	5.832252746505022E-5	3.888168497670015E-5
10-11	1.1664505493010044E-4	1.0692463368592541E-4	0.0	5.832252746505022E-5	3.888168497670015E-5
12-13	1.1664505493010044E-4	1.1664505493010044E-4	0.0	5.832252746505022E-5	3.888168497670015E-5
14-15	1.263654761742755E-4	1.555267399068006E-4	0.0	5.832252746505022E-5	4.860210622087519E-5
16-17	2.1384926737185082E-4	1.555267399068006E-4	0.0	5.832252746505022E-5	7.77633699534003E-5
18-19	2.1384926737185082E-4	1.6524716115097563E-4	0.0	5.832252746505022E-5	7.77633699534003E-5
20-21	2.1384926737185082E-4	1.9440842488350075E-4	0.0	7.77633699534003E-5	8.748379119757534E-5
22-23	2.1384926737185082E-4	2.1384926737185082E-4	0.0	1.0692463368592541E-4	1.0692463368592541E-4
24-25	2.1384926737185082E-4	2.2356968861602587E-4	0.0	1.555267399068006E-4	1.1664505493010044E-4
26-27	2.1384926737185082E-4	2.332901098602009E-4	0.0	1.555267399068006E-4	1.1664505493010044E-4
28-29	2.1384926737185082E-4	2.332901098602009E-4	0.0	3.6937600727865146E-4	1.263654761742755E-4
30-31	2.1384926737185082E-4	2.7217179483690103E-4	0.0	0.0010595259156150792	1.555267399068006E-4
32-33	2.1384926737185082E-4	2.916126373252511E-4	0.0	0.0024689869960204595	1.7496758239515068E-4
34-35	2.1384926737185082E-4	3.3049432230195127E-4	0.0	0.004481114193564692	1.7496758239515068E-4
36-37	2.1384926737185082E-4	3.693760072786514E-4	0.0	0.007251434248154578	1.7496758239515068E-4
38-39	2.1384926737185082E-4	3.888168497670015E-4	0.0	0.011703387177986746	2.0412884612767577E-4
40-41	2.1384926737185082E-4	4.665802197204018E-4	0.0	0.016660802012516015	2.1384926737185082E-4
42-43	2.1384926737185082E-4	4.763006409645768E-4	0.0	0.021132195784836532	2.1384926737185082E-4
44-45	2.2356968861602587E-4	5.34623168429627E-4	0.0	0.02650758873286533	2.1384926737185082E-4
46-47	2.332901098602009E-4	5.832252746505023E-4	0.0	0.0329425075965092	2.332901098602009E-4
48-49	2.332901098602009E-4	5.929456958946773E-4	0.0	0.0394065877238856	2.52730952348551E-4
50-51	2.332901098602009E-4	6.609886446039025E-4	0.0	0.046521936074621725	2.52730952348551E-4
52-53	2.7217179483690103E-4	6.609886446039025E-4	0.0	0.053306790103055904	2.52730952348551E-4
54-55	2.7217179483690103E-4	6.609886446039025E-4	0.0	0.06015968708019931	2.52730952348551E-4
56-57	2.7217179483690103E-4	6.609886446039025E-4	0.0	0.06473800548620576	2.62451373592726E-4
58-59	2.7217179483690103E-4	6.804294870922527E-4	0.0	0.06977318369068843	2.7217179483690103E-4
60-61	2.8189221608107605E-4	6.998703295806027E-4	0.0	0.0748375231589036	2.7217179483690103E-4
62-63	2.916126373252511E-4	6.998703295806027E-4	0.0	0.08022263652817659	3.3049432230195127E-4
64-65	2.916126373252511E-4	8.748379119757534E-4	0.0	0.08459682608805535	3.3049432230195127E-4
66-67	2.916126373252511E-4	8.748379119757534E-4	0.0	0.08966116555627054	3.3049432230195127E-4
68-69	2.916126373252511E-4	8.942787544641035E-4	0.0	0.09435612901720708	3.3049432230195127E-4
70-71	3.013330585694262E-4	9.137195969524535E-4	0.0	0.09859423267966741	3.3049432230195127E-4
72-73	3.3049432230195127E-4	9.137195969524535E-4	0.0	0.10227827233120974	3.693760072786514E-4
74-75	3.3049432230195127E-4	9.234400181966285E-4	0.0	0.10514579659824139	3.693760072786514E-4
76-77	3.3049432230195127E-4	9.720421244175039E-4	0.0	0.10795499833780797	3.693760072786514E-4
78-79	3.3049432230195127E-4	9.91482966905854E-4	0.0	0.10968523331927113	3.693760072786514E-4
80-81	3.3049432230195127E-4	9.91482966905854E-4	0.0	0.11024901775143328	3.985372710111765E-4
82-83	3.402147435461263E-4	9.91482966905854E-4	0.0	0.11085168386857214	4.5685979847622676E-4
84-85	3.4993516479030136E-4	9.91482966905854E-4	0.0	0.11120161903336243	4.665802197204018E-4
86-87	3.4993516479030136E-4	9.91482966905854E-4	0.0	0.11135714577326923	4.665802197204018E-4
88	3.4993516479030136E-4	0.001010923809394204	0.0	0.11135714577326923	4.665802197204018E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6945	0.0	49.726555	1
GTATCAA	12520	0.0	38.006084	1
ATCAACG	15260	0.0	31.379612	3
TCAACGC	15345	0.0	31.205793	4
CAACGCA	15675	0.0	30.520788	5
TATCAAC	16225	0.0	29.836687	2
AACGCAG	16115	0.0	29.714443	6
ACGCAGA	18580	0.0	25.79565	7
CGCAGAG	18800	0.0	25.40125	8
GTGGTAT	3435	0.0	23.678547	1
TGGTATC	3415	0.0	23.307758	2
GCAGAGT	21890	0.0	21.835468	9
GAGTACT	13910	0.0	20.354687	12-13
CAGAGTA	21580	0.0	19.388054	10-11
GTACATG	14175	0.0	19.14704	1
AGAGTAC	19770	0.0	19.128174	10-11
TACATGG	13865	0.0	19.104582	2
ACATGGG	14195	0.0	18.13849	3
GTACTTT	15605	0.0	17.962795	14-15
AGTACTT	15130	0.0	17.247372	12-13
>>END_MODULE