FastQCFastQC Report
Fri 27 May 2016
SRR2140128_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140128_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5835327
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75400.046.7697641
CTTATAC55550.039.2254451
GTATCAA135800.037.334741
TATACAC80200.031.3131893
ATCAACG163900.030.7485683
TCAACGC164300.030.699664
CAACGCA168350.029.8846265
TATCAAC170650.029.6852842
AACGCAG174250.028.9216846
TTATACA86150.027.7676162
ACGCAGA199500.025.2611737
CGCAGAG202100.024.936198
ACACATC102400.022.4839576
ACATCTC105750.022.0136078
TACACAT112450.021.877385
GCAGAGT237500.021.2373379
GTGGTAT38700.021.0452181
TGGTATC37350.020.8925532
CACATCT111600.020.5158277
GAGTACT136100.019.48548512-13
Produced by FastQC (version 0.11.5)