##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140128_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5835327
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.518103612702426	18.0	18.0	30.0	18.0	33.0
2	30.292635528394552	31.0	29.0	33.0	27.0	33.0
3	31.46205414023927	33.0	31.0	33.0	29.0	33.0
4	32.47908609063382	33.0	33.0	33.0	31.0	34.0
5	33.0576435562223	33.0	33.0	34.0	33.0	34.0
6	37.01430528229181	38.0	38.0	38.0	35.0	38.0
7	37.17476467042892	38.0	38.0	38.0	36.0	38.0
8	37.14234626439958	38.0	38.0	38.0	36.0	38.0
9	37.36822375164237	38.0	38.0	38.0	37.0	38.0
10-11	37.38167244097889	38.0	38.0	38.0	37.0	38.0
12-13	37.43659027163345	38.0	38.0	38.0	37.0	38.0
14-15	37.402747952257	38.0	38.0	38.0	37.0	38.0
16-17	37.41339397089486	38.0	38.0	38.0	37.0	38.0
18-19	37.44925220129052	38.0	38.0	38.0	37.0	38.0
20-21	37.466304591205635	38.0	38.0	38.0	37.0	38.0
22-23	37.48708660433187	38.0	38.0	38.0	37.0	38.0
24-25	37.4982261574433	38.0	38.0	38.0	37.0	38.0
26-27	37.46769120058739	38.0	38.0	38.0	37.0	38.0
28-29	37.43942932554761	38.0	38.0	38.0	37.0	38.0
30-31	37.442090432741296	38.0	38.0	38.0	37.0	38.0
32-33	37.430978020442055	38.0	38.0	38.0	37.0	38.0
34-35	37.39697344494702	38.0	38.0	38.0	37.0	38.0
36-37	37.37089178139888	38.0	38.0	38.0	37.0	38.0
38-39	37.338441090228216	38.0	38.0	38.0	37.0	38.0
40-41	37.36060535178227	38.0	38.0	38.0	37.0	38.0
42-43	37.33243730931754	38.0	38.0	38.0	37.0	38.0
44-45	37.29165419757739	38.0	38.0	38.0	37.0	38.0
46-47	37.25652489861025	38.0	38.0	38.0	37.0	38.0
48-49	37.209929048275484	38.0	38.0	38.0	37.0	38.0
50-51	37.17060538111439	38.0	38.0	38.0	37.0	38.0
52-53	37.12795296652682	38.0	38.0	38.0	36.5	38.0
54-55	37.110348683229475	38.0	38.0	38.0	36.0	38.0
56-57	37.06792729569088	38.0	38.0	38.0	36.0	38.0
58-59	37.03353132549027	38.0	38.0	38.0	36.0	38.0
60-61	37.04856148910562	38.0	38.0	38.0	36.0	38.0
62-63	37.059465215823934	38.0	38.0	38.0	36.0	38.0
64-65	37.06639839537149	38.0	38.0	38.0	36.0	38.0
66-67	37.05497747518194	38.0	38.0	38.0	36.0	38.0
68-69	37.01581935005109	38.0	38.0	38.0	36.0	38.0
70-71	37.01117270407869	38.0	38.0	38.0	36.0	38.0
72-73	37.02045193518979	38.0	38.0	38.0	36.0	38.0
74-75	37.03404329486078	38.0	38.0	38.0	36.0	38.0
76-77	37.01430259682198	38.0	38.0	38.0	36.0	38.0
78-79	36.976489790781976	38.0	38.0	38.0	36.0	38.0
80-81	36.92880289596426	38.0	38.0	38.0	36.0	38.0
82-83	36.88812558958813	38.0	38.0	38.0	35.0	38.0
84-85	36.85867016014517	38.0	38.0	38.0	35.0	38.0
86-87	36.87974464947428	38.0	38.0	38.0	35.0	38.0
88-89	36.836534753303994	38.0	38.0	38.0	35.0	38.0
90-91	36.84785779231077	38.0	38.0	38.0	35.0	38.0
92-93	36.83418711172398	38.0	38.0	38.0	35.0	38.0
94-95	36.847047973644464	38.0	38.0	38.0	35.0	38.0
96-97	36.84732316011704	38.0	38.0	38.0	35.0	38.0
98-99	36.82362125547757	38.0	38.0	38.0	35.0	38.0
100	35.22915125081729	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	16.0
20	28.0
21	80.0
22	271.0
23	905.0
24	2294.0
25	4754.0
26	8568.0
27	14528.0
28	23611.0
29	33935.0
30	46362.0
31	60668.0
32	78117.0
33	105948.0
34	160207.0
35	292497.0
36	939035.0
37	4063499.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.600381092610576	19.691321497492773	16.400777539973337	23.30751986992331
2	14.339751664567213	20.823328791657026	42.28316066394166	22.553758879834103
3	18.068963129877517	25.73849836195815	31.066105874895317	25.12643263326902
4	12.451748088046106	18.21133621957955	38.40760507541993	30.92931061695441
5	13.369447847567068	37.26199063051651	35.72941156511023	13.63914995680619
6	29.940772813588683	37.667434918385894	18.698540801569475	13.69325146645595
7	26.480898157035586	32.63578887695582	22.669714996263277	18.21359796974531
8	24.229267699993507	36.899320295160834	21.13341720181234	17.737994803033317
9	25.168015434267865	17.292518482683146	21.14087179690187	36.398594286147116
10-11	24.123506703223317	26.76784865698186	28.793527766310266	20.315116873484556
12-13	24.984401045562656	24.08960114831611	28.979258574540896	21.946739231580338
14-15	22.170779803771065	25.59485526689421	26.573874951652236	25.66048997768248
16-17	21.780647425585574	28.96733979089775	27.169994072311628	22.082018711205045
18-19	21.75195151874094	27.800455741383473	29.034739612707224	21.412853127168365
20-21	22.498280065268837	26.89707151389645	28.973297718752605	21.631350702082113
22-23	22.17099299253718	27.02866764118932	28.89187451537007	21.908464850903435
24-25	22.044946233186597	27.16290346533996	29.043899973494298	21.748250327979147
26-27	22.02568026141017	27.274144538376323	29.004797307871566	21.695377892341945
28-29	21.642328625881095	27.145347667802334	29.16434106214238	22.04798264417419
30-31	22.23977455662692	26.879091676992537	29.241654195384086	21.63947957099646
32-33	21.80002424657216	27.34134616624722	28.988496635143257	21.87013295203736
34-35	21.873073411387615	27.33287037739414	29.275535445092828	21.51852076612542
36-37	22.118590565244208	27.116316409815873	28.989291267269113	21.775801757670806
38-39	21.80016116582237	27.082513229890615	29.194250053044833	21.92307555124218
40-41	22.043527708035864	26.948495193803172	29.13532219014765	21.872654908013313
42-43	22.10851935427449	26.969515117557318	29.04241173535252	21.87955379281567
44-45	21.96935537764377	27.288564722466663	28.8342465819057	21.907833317983872
46-47	22.26160495841247	26.973811439969435	28.40335793036627	22.361225671251827
48-49	22.129298115978564	27.051321381873848	28.533552184992107	22.285828317155477
50-51	21.92369291862104	27.348996341565936	28.591246324007052	22.13606441580598
52-53	22.118706883269496	27.33966536188151	28.60184267181823	21.93978508303077
54-55	22.04445064225877	27.313327527499982	28.390303457918638	22.251918372322613
56-57	22.2824142865325	27.580232760101943	28.06995748144499	22.06739547192057
58-59	21.938116417942695	27.877852426544187	28.41486322021407	21.769167935299045
60-61	22.144585509651094	28.12501480461415	27.997557736100198	21.732841949634558
62-63	21.930889464958188	28.282077409527435	27.981309199222565	21.805723926291808
64-65	21.900525151529795	28.219580725685855	28.03495827137051	21.844935851413844
66-67	21.99127154159076	28.142218229000953	27.94781829955364	21.91869192985465
68-69	21.99357327706986	27.92023081245325	28.157928688271806	21.928267222205083
70-71	22.065007091840556	27.457509564282546	28.182646145938577	22.29483719793832
72-73	22.172014712512908	27.285952248975022	28.35585769392996	22.186175344582114
74-75	22.067988313333082	27.480080541316266	28.2782453525171	22.173685792833552
76-77	22.211453924046307	27.46669792858244	28.06597156249813	22.255876584873118
78-79	22.177275315019944	27.547117319536955	28.02513131635733	22.250476049085766
80-81	22.223147778645327	27.496872570523813	27.99281791558834	22.28716173524252
82-83	22.256081897292948	27.484613473905007	28.09892809147632	22.160376537325728
84-85	22.386478437767877	27.463719979396256	27.996315676317117	22.153485906518753
86-87	22.151484123808675	27.478349369174193	28.080457738724036	22.289708768293092
88-89	22.2845442831881	27.519721336441698	27.955018326826714	22.240716053543487
90-91	22.323496462814976	27.53045348868962	27.99196965192649	22.154080396568915
92-93	22.351932742899074	27.50631250746166	27.97577660497766	22.16597814466161
94-95	22.215572071144205	27.606660120023797	27.938148922031115	22.239618886800887
96-97	22.331348798354227	27.692304334370515	28.007813460298237	21.96853340697702
98-99	23.404634209220436	29.63931313920063	28.189688444876932	18.766364206702004
100	22.288836780619224	30.53963569142218	22.113982317615953	25.057545210342646
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1150.0
1	1081.0
2	1265.5
3	1657.5
4	2199.5
5	3191.0
6	4076.0
7	5258.0
8	6459.5
9	6948.5
10	6916.0
11	6860.0
12	7649.5
13	9716.5
14	13770.0
15	18410.5
16	21844.0
17	23577.5
18	23237.0
19	21468.0
20	19440.0
21	17877.0
22	18095.0
23	20674.0
24	25884.5
25	33829.0
26	44433.5
27	56728.0
28	68079.0
29	80225.5
30	92295.5
31	103844.0
32	117696.5
33	132193.5
34	146652.0
35	160054.5
36	175459.5
37	190190.5
38	199617.0
39	207926.5
40	218632.5
41	228468.5
42	236432.0
43	250044.5
44	264595.0
45	271174.5
46	268796.5
47	262621.5
48	253873.0
49	245033.5
50	238934.5
51	229585.0
52	217841.0
53	205833.0
54	192165.0
55	176087.5
56	160264.0
57	144106.0
58	127832.0
59	111799.0
60	93359.5
61	75157.5
62	58999.5
63	45374.5
64	34273.0
65	25489.0
66	18719.5
67	14083.5
68	10650.5
69	7755.0
70	5639.0
71	3969.5
72	2775.0
73	1851.0
74	1213.5
75	821.5
76	536.0
77	339.5
78	206.0
79	128.5
80	88.5
81	63.0
82	43.0
83	27.5
84	19.5
85	12.5
86	7.5
87	7.5
88	6.0
89	4.0
90	4.5
91	3.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008174349098173932
3	1.0282200123489225E-4
4	0.0011310420135838147
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	4.284786813002991E-5
26-27	4.970923525598371E-4
28-29	0.0011317099468559297
30-31	4.977332113944176E-4
32-33	5.416758056546312E-4
34-35	9.91299749994203E-4
36-37	0.0
38-39	1.4789025846867468E-4
40-41	7.877083882541126E-4
42-43	3.5228056751694865E-4
44-45	0.0
46-47	0.0
48-49	0.0016335987765879482
50-51	0.003320610996062301
52-53	0.0
54-55	0.003703159533867333
56-57	0.03509773135526119
58-59	0.05155793023125671
60-61	0.0280853457296587
62-63	0.00651931049401557
64-65	0.0
66-67	0.0
68-69	6.89921624903411E-5
70-71	0.003721119919424286
72-73	0.007473669454547365
74-75	0.005280517286813132
76-77	0.006339810987180615
78-79	0.009574126033545616
80-81	0.010681857521456143
82-83	0.038772381925151686
84-85	0.02328418438116628
86-87	9.848354816094419E-4
88-89	0.001465293591800917
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	42.0
20-21	203.0
22-23	389.0
24-25	551.0
26-27	1497.0
28-29	3751.0
30-31	10579.0
32-33	13723.0
34-35	18715.0
36-37	29555.0
38-39	35007.0
40-41	34727.0
42-43	39644.0
44-45	47510.0
46-47	48070.0
48-49	44956.0
50-51	41446.0
52-53	40061.0
54-55	42982.0
56-57	45561.0
58-59	45294.0
60-61	48725.0
62-63	46794.0
64-65	51305.0
66-67	56821.0
68-69	57498.0
70-71	67167.0
72-73	46758.0
74-75	45961.0
76-77	47811.0
78-79	49449.0
80-81	48889.0
82-83	46240.0
84-85	45996.0
86-87	47128.0
88-89	49219.0
90-91	50275.0
92-93	49253.0
94-95	57742.0
96-97	178198.0
98-99	391525.0
100-101	3808310.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.98811613137762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.07783029010085	77.45622931847717
2	7.096955534853325	12.205076734203818
3	1.6100617398259223	4.153385275885179
4	0.5802720488853214	1.9958600130937383
5	0.2619822480111761	1.1263679983172192
6	0.13446394663735448	0.693738087536172
7	0.0720804581239574	0.43386439627760254
8	0.046061191733686446	0.3168572083156705
9	0.030960824302868926	0.23960366601104444
>10	0.08726001853896638	1.1089465593126093
>50	0.001303918898325795	0.07251725421360598
>100	6.771063199107194E-4	0.1236391949126134
>500	6.04491802905874E-5	0.03862047921446896
>1k	3.0224588109982572E-5	0.03529381422912113
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	5.1411000617446117E-5	0.0	0.0	1.713700020581537E-5
5	0.0	5.1411000617446117E-5	0.0	0.0	3.427400041163074E-5
6	0.0	5.1411000617446117E-5	0.0	0.0	3.427400041163074E-5
7	0.0	5.1411000617446117E-5	0.0	0.0	5.1411000617446117E-5
8	0.0	5.1411000617446117E-5	0.0	0.0	5.1411000617446117E-5
9	0.0	1.199590014407076E-4	0.0	1.713700020581537E-5	5.1411000617446117E-5
10-11	0.0	1.199590014407076E-4	0.0	1.713700020581537E-5	1.0282200123489223E-4
12-13	0.0	1.199590014407076E-4	0.0	1.713700020581537E-5	1.0282200123489223E-4
14-15	0.0	2.9989750360176904E-4	0.0	1.713700020581537E-5	1.1139050133779992E-4
16-17	0.0	3.084660037046767E-4	0.0	1.713700020581537E-5	1.199590014407076E-4
18-19	0.0	3.341715040133998E-4	0.0	1.713700020581537E-5	1.199590014407076E-4
20-21	0.0	3.5987700432212283E-4	0.0	2.5705500308723058E-5	1.3709600164652297E-4
22-23	0.0	3.855825046308459E-4	0.0	5.1411000617446117E-5	1.4566450174943068E-4
24-25	0.0	4.36993505248292E-4	0.0	5.1411000617446117E-5	1.6280150195524605E-4
26-27	0.0	4.798360057628304E-4	0.0	9.425350113198455E-5	1.7993850216106141E-4
28-29	0.0	4.798360057628304E-4	0.0	4.712675056599228E-4	1.885070022639691E-4
30-31	0.0	5.483840065860919E-4	0.0	0.0013880970166710452	1.885070022639691E-4
32-33	0.0	5.483840065860919E-4	0.0	0.003461674041574705	1.885070022639691E-4
34-35	0.0	6.512060078209842E-4	0.0	0.006220731074710981	2.0564400246978447E-4
36-37	0.0	7.36891008850061E-4	0.0	0.01052211812637064	2.0564400246978447E-4
38-39	0.0	7.540280090558764E-4	0.0	0.016597184699332187	2.0564400246978447E-4
40-41	0.0	7.883020094675071E-4	0.0	0.023374868280732167	2.0564400246978447E-4
42-43	0.0	8.054390096733225E-4	0.0	0.029389955352973365	2.0564400246978447E-4
44-45	0.0	8.654185103936763E-4	0.0	0.036287597935814056	2.0564400246978447E-4
46-47	0.0	9.168295110111224E-4	0.0	0.04399067952832807	2.0564400246978447E-4
48-49	0.0	9.253980111140301E-4	0.0	0.051847994122694405	2.1421250257269215E-4
50-51	0.0	0.0010282200123489223	0.0	0.059525370214899696	2.2278100267559983E-4
52-53	0.0	0.0010453570125547376	0.0	0.06705708180535555	2.570550030872306E-4
54-55	0.0	0.0010453570125547376	0.0	0.07481157439848701	2.9132900349886136E-4
56-57	0.0	0.0010453570125547376	0.0	0.08251465599100102	2.9989750360176904E-4
58-59	0.0	0.001062494012760553	0.0	0.08978074407826674	3.084660037046767E-4
60-61	0.0	0.0010796310129663684	0.0	0.09691830466398885	3.256030039104921E-4
62-63	0.0	0.0010796310129663684	0.0	0.10343036474219869	3.4274000411630746E-4
64-65	0.0	0.0011139050133779991	0.0	0.10995956182061434	3.4274000411630746E-4
66-67	0.0	0.0011310420135838145	0.0	0.11648875889902999	3.4274000411630746E-4
68-69	0.0	0.0011824530142012608	0.0	0.12287229147569623	3.4274000411630746E-4
70-71	0.0	0.0011995900144070762	0.0	0.1278591585355885	3.770140045279382E-4
72-73	0.0	0.0012081585145099839	0.0	0.13204058658580745	4.1128800493956893E-4
74-75	0.0	0.001233864014818707	0.0	0.13519379462367748	4.284250051453843E-4
76-77	0.0	0.0012938435155390607	0.0	0.13815849565928354	4.36993505248292E-4
78-79	0.0	0.0013195490158477837	0.0	0.1400092916815116	4.4556200535119967E-4
80-81	0.0	0.0013195490158477837	0.0	0.14066906618943548	4.5413050545410735E-4
82-83	0.0	0.0013281175159506914	0.0	0.1411317651949925	4.798360057628304E-4
84-85	0.0	0.0013538230162594145	0.0	0.14136311469777102	4.798360057628304E-4
86-87	0.0	0.0013709600164652298	0.0	0.1414659366990059	4.798360057628304E-4
88	0.0	0.0013709600164652298	0.0	0.1414659366990059	4.969730059686458E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7540	0.0	46.769764	1
CTTATAC	5555	0.0	39.225445	1
GTATCAA	13580	0.0	37.33474	1
TATACAC	8020	0.0	31.313189	3
ATCAACG	16390	0.0	30.748568	3
TCAACGC	16430	0.0	30.69966	4
CAACGCA	16835	0.0	29.884626	5
TATCAAC	17065	0.0	29.685284	2
AACGCAG	17425	0.0	28.921684	6
TTATACA	8615	0.0	27.767616	2
ACGCAGA	19950	0.0	25.261173	7
CGCAGAG	20210	0.0	24.93619	8
ACACATC	10240	0.0	22.483957	6
ACATCTC	10575	0.0	22.013607	8
TACACAT	11245	0.0	21.87738	5
GCAGAGT	23750	0.0	21.237337	9
GTGGTAT	3870	0.0	21.045218	1
TGGTATC	3735	0.0	20.892553	2
CACATCT	11160	0.0	20.515827	7
GAGTACT	13610	0.0	19.485485	12-13
>>END_MODULE