FastQCFastQC Report
Fri 27 May 2016
SRR2140127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5681334
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70350.048.7288671
GTATCAA128950.038.467791
TCAACGC161600.031.4249554
ATCAACG162400.031.2431963
TATCAAC168050.030.559632
CAACGCA166300.030.4841695
AACGCAG171450.029.5684876
ACGCAGA196900.025.7911287
CGCAGAG199000.025.4969628
CTTATAC36800.025.3408051
GTGGTAT34350.025.1089251
TGGTATC33850.024.5744362
GCAGAGT234300.021.6742349
TACATGG162050.020.1547992
TATACAC59400.020.120293
GTACATG163900.019.9273011
GAGTACT145800.019.80228412-13
ACATGGG162600.019.6813493
AGAGTAC211750.019.49603510-11
CAGAGTA227150.018.8777310-11
Produced by FastQC (version 0.11.5)