##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140127_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5681334
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.56649353831336	18.0	18.0	30.0	18.0	33.0
2	29.927134014652193	31.0	27.0	33.0	27.0	33.0
3	31.211195997278104	33.0	31.0	33.0	29.0	33.0
4	32.40806507767366	33.0	33.0	33.0	31.0	34.0
5	33.016432056274105	33.0	33.0	34.0	33.0	34.0
6	36.99451889292198	38.0	37.0	38.0	35.0	38.0
7	37.12851277534467	38.0	38.0	38.0	36.0	38.0
8	37.095435156602306	38.0	38.0	38.0	36.0	38.0
9	37.34376292610151	38.0	38.0	38.0	37.0	38.0
10-11	37.364446624683566	38.0	38.0	38.0	37.0	38.0
12-13	37.42315044318816	38.0	38.0	38.0	37.0	38.0
14-15	37.385610228161205	38.0	38.0	38.0	37.0	38.0
16-17	37.40173901059153	38.0	38.0	38.0	37.0	38.0
18-19	37.44803491574338	38.0	38.0	38.0	37.0	38.0
20-21	37.46357297524061	38.0	38.0	38.0	37.0	38.0
22-23	37.48507411435064	38.0	38.0	38.0	37.0	38.0
24-25	37.496954668760864	38.0	38.0	38.0	37.0	38.0
26-27	37.46776577460807	38.0	38.0	38.0	37.0	38.0
28-29	37.4429287534987	38.0	38.0	38.0	37.0	38.0
30-31	37.44384432472337	38.0	38.0	38.0	37.0	38.0
32-33	37.43226561401174	38.0	38.0	38.0	37.0	38.0
34-35	37.40389324312255	38.0	38.0	38.0	37.0	38.0
36-37	37.3757528777603	38.0	38.0	38.0	37.0	38.0
38-39	37.3531180619129	38.0	38.0	38.0	37.0	38.0
40-41	37.34948281810805	38.0	38.0	38.0	37.0	38.0
42-43	37.31723558516333	38.0	38.0	38.0	37.0	38.0
44-45	37.27384744873338	38.0	38.0	38.0	37.0	38.0
46-47	37.23496909675833	38.0	38.0	38.0	37.0	38.0
48-49	37.185528846497874	38.0	38.0	38.0	37.0	38.0
50-51	37.13877323508114	38.0	38.0	38.0	36.5	38.0
52-53	37.09624051932729	38.0	38.0	38.0	36.0	38.0
54-55	37.07313330321202	38.0	38.0	38.0	36.0	38.0
56-57	37.02591561148689	38.0	38.0	38.0	36.0	38.0
58-59	36.99515575875084	38.0	38.0	38.0	36.0	38.0
60-61	37.0149145179965	38.0	38.0	38.0	36.0	38.0
62-63	37.02485678132005	38.0	38.0	38.0	36.0	38.0
64-65	37.02938257988135	38.0	38.0	38.0	36.0	38.0
66-67	37.02387059772231	38.0	38.0	38.0	36.0	38.0
68-69	36.98417503910606	38.0	38.0	38.0	36.0	38.0
70-71	36.97770735607084	38.0	38.0	38.0	36.0	38.0
72-73	36.987145911709554	38.0	38.0	38.0	36.0	38.0
74-75	37.00051221376484	38.0	38.0	38.0	36.0	38.0
76-77	36.977046189403204	38.0	38.0	38.0	36.0	38.0
78-79	36.93433279281419	38.0	38.0	38.0	35.5	38.0
80-81	36.88613670868369	38.0	38.0	38.0	35.0	38.0
82-83	36.84038209446591	38.0	38.0	38.0	35.0	38.0
84-85	36.80708313915725	38.0	38.0	38.0	35.0	38.0
86-87	36.82784394448917	38.0	38.0	38.0	35.0	38.0
88-89	36.78011089167242	38.0	38.0	38.0	35.0	38.0
90-91	36.79246023665547	38.0	38.0	38.0	35.0	38.0
92-93	36.77697919886762	38.0	38.0	38.0	35.0	38.0
94-95	36.789785176192936	38.0	38.0	38.0	35.0	38.0
96-97	36.78421715441644	38.0	38.0	38.0	35.0	38.0
98-99	36.76148172803859	38.0	38.0	38.0	34.0	38.0
100	35.10143092873549	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	5.0
20	29.0
21	76.0
22	327.0
23	906.0
24	2249.0
25	4640.0
26	8655.0
27	14498.0
28	22825.0
29	34328.0
30	47069.0
31	60619.0
32	78779.0
33	106199.0
34	160683.0
35	297263.0
36	931336.0
37	3910844.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.37120507261147	19.405882491682412	17.196964656540175	23.02594777916595
2	14.517391889065934	20.93157732791356	42.00791983839249	22.543110944628022
3	18.331021375096785	25.546126427064365	30.900118299743497	25.222733898095356
4	12.691882476422114	17.905164631120783	38.51051444948028	30.892438442976815
5	13.554862291145003	37.082628833298656	35.56217606639567	13.800332809160665
6	30.132676586167968	37.54070786896176	18.68645638506731	13.64015915980296
7	26.674281075536133	32.59259181030371	22.777273788163132	17.95585332599703
8	24.260446578215607	36.92132868794547	21.097826672397716	17.7203980614412
9	25.407078689617613	17.23598718188369	21.287658849136488	36.06927527936221
10-11	24.292243687838102	26.73598313353871	28.718528078088703	20.253245100534485
12-13	25.114893790789278	24.118173654286124	29.054611117741008	21.71232143718359
14-15	22.187394721028546	25.58367453840947	26.73027144681161	25.498659293750375
16-17	21.74141671656692	28.78877566430701	27.41919415404903	22.050613465077042
18-19	21.76140321973677	27.7419704597547	29.364960764496505	21.13166555601202
20-21	22.46272489681888	26.907231338334093	29.329382574888157	21.30066118995887
22-23	22.20049900806547	26.84090964898253	29.214423584428324	21.74416775852368
24-25	22.055884156509016	26.913945179790606	29.30068801854549	21.729482645154892
26-27	21.918698591575083	27.115630203339787	29.272596644581945	21.693074560503188
28-29	21.813448201117463	27.134740678511367	29.32461764688792	21.727193473483254
30-31	22.146697600392937	27.050937586820528	29.171424086553362	21.630940726233174
32-33	22.067326815787037	27.075478377969187	29.12992423530993	21.727270570933847
34-35	22.063135066019935	27.07697660228476	29.15683630606264	21.703052025632672
36-37	21.97916905387588	27.095608381539844	29.18616214712476	21.739060417459516
38-39	21.85563427128611	27.15364011018881	29.25547111999342	21.735254498531663
40-41	22.126694805312216	27.00823646366932	29.086373445080337	21.77869528593812
42-43	22.0733231259708	27.063476991052642	28.963489544606425	21.899710338370134
44-45	22.050238855749168	27.2376139903509	28.834920823725767	21.877226330174164
46-47	22.202653958891286	27.115459681004477	28.536193305648023	22.145693054456213
48-49	22.09052934562561	27.252382595141135	28.534432712067055	22.122655347166205
50-51	22.03472758756545	27.40974467341719	28.511891752814687	22.043635986202673
52-53	22.262180757169396	27.39735599684715	28.368858016597287	21.97160522938617
54-55	22.141199298426184	27.253405331650892	28.414893196793965	22.190502173128955
56-57	22.24377811203024	27.45914085522683	28.248234350731927	22.048846682011003
58-59	22.11271610480678	27.536262750414792	28.353686730862677	21.997334413915752
60-61	22.194014239034143	27.519093942167277	28.363895948714145	21.92299587008443
62-63	22.073938642620192	27.652408116385185	28.314429114519623	21.959224126475
64-65	22.047534020270245	27.543825139273277	28.35936614204086	22.049274698415623
66-67	22.11239344219575	27.58322138404765	28.251477663506797	22.052907510249803
68-69	22.081027464349344	27.520253196461436	28.371780229195174	22.026939109994043
70-71	22.117327123380736	27.13746298297431	28.395944468812985	22.34926542483197
72-73	22.18078713825659	27.164547702140418	28.44239901278686	22.21226614681613
74-75	22.070618688159463	27.439100483645312	28.34269809976903	22.147582728426194
76-77	22.20110358010691	27.367845040348133	28.157371931672152	22.2736794478728
78-79	22.169894212567286	27.417782552894472	28.141637892073216	22.270685342465026
80-81	22.24927134796719	27.430895008553453	28.053017008070828	22.26681663540852
82-83	22.26825381986089	27.42574055675012	28.1179484286141	22.188057194774892
84-85	22.36888038871533	27.368306823178983	28.073446538271273	22.18936624983441
86-87	22.166149044069456	27.464371925216604	28.077855186878313	22.291623843835627
88-89	22.279530976049998	27.440661004012934	28.006519990356015	22.273288029581057
90-91	22.31397134398972	27.435291750178415	28.045895127781996	22.204841778049865
92-93	22.31060847659757	27.4583338916507	27.966286564204264	22.264771067547468
94-95	22.225464692370643	27.57893094892622	27.938173532452776	22.25743082625036
96-97	22.313711215267773	27.63691090206463	28.05338969816807	21.995988184499524
98-99	23.37324715941727	29.5745520282562	28.222068390200427	18.830132422126102
100	22.302431601395373	30.486820313876116	22.21755132203925	24.993196762689262
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1218.0
1	1159.0
2	1376.5
3	1770.5
4	2220.0
5	3013.5
6	3888.0
7	5563.5
8	7274.0
9	7953.5
10	7895.5
11	7654.0
12	8279.5
13	10094.0
14	14002.0
15	18618.5
16	21862.0
17	23338.0
18	22758.5
19	20892.0
20	18618.0
21	16631.0
22	16563.5
23	18932.5
24	23715.5
25	31085.0
26	41143.0
27	52500.5
28	63067.5
29	74454.5
30	86193.0
31	98047.5
32	112012.0
33	125784.5
34	138660.5
35	150819.0
36	164024.5
37	176602.0
38	186042.5
39	193649.0
40	202870.5
41	212178.5
42	218659.5
43	227149.0
44	233815.5
45	236884.5
46	237472.0
47	235394.0
48	233074.0
49	229120.0
50	223346.5
51	216035.5
52	206494.0
53	195407.5
54	183302.5
55	169635.5
56	154532.0
57	139055.0
58	123760.0
59	107201.5
60	89165.5
61	71644.0
62	55470.0
63	42053.5
64	31421.5
65	22821.0
66	16779.0
67	12719.5
68	9514.5
69	6859.5
70	4846.5
71	3434.5
72	2373.0
73	1610.0
74	1061.0
75	630.5
76	367.5
77	217.5
78	135.0
79	82.0
80	48.0
81	31.0
82	22.0
83	15.0
84	9.5
85	7.5
86	6.5
87	4.0
88	2.0
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007216614971061374
3	5.280449978825395E-5
4	0.0010032854959768252
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	9.681838929758961E-5
26-27	5.105386623006809E-4
28-29	0.0011093166398200302
30-31	5.197092693495469E-4
32-33	6.26055807849188E-4
34-35	0.0010684580499086469
36-37	0.0
38-39	2.840184168192156E-4
40-41	8.817929005161249E-4
42-43	3.308261775780144E-4
44-45	0.0
46-47	0.0
48-49	0.0017721242671698168
50-51	0.003535823972612439
52-53	0.0
54-55	0.003728494707014148
56-57	0.03739212524997689
58-59	0.05354272163022323
60-61	0.027952756460200432
62-63	0.006795265089528798
64-65	9.511901528990611E-6
66-67	0.0
68-69	4.8284527976248645E-5
70-71	0.003447402090372185
72-73	0.007414942021537117
74-75	0.005472391044991169
76-77	0.006413316398720369
78-79	0.009504846767788849
80-81	0.010237930935465827
82-83	0.039591130481332136
84-85	0.02410355921310292
86-87	0.001010035904173208
88-89	0.0014398507599942196
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	37.0
20-21	154.0
22-23	329.0
24-25	386.0
26-27	840.0
28-29	2044.0
30-31	5531.0
32-33	7353.0
34-35	10177.0
36-37	16146.0
38-39	19793.0
40-41	20670.0
42-43	24854.0
44-45	31144.0
46-47	32348.0
48-49	30084.0
50-51	27583.0
52-53	26962.0
54-55	29518.0
56-57	31768.0
58-59	32045.0
60-61	32909.0
62-63	33328.0
64-65	36668.0
66-67	40481.0
68-69	42240.0
70-71	45129.0
72-73	38672.0
74-75	38918.0
76-77	41298.0
78-79	43199.0
80-81	43405.0
82-83	41439.0
84-85	41369.0
86-87	43645.0
88-89	46136.0
90-91	46899.0
92-93	47158.0
94-95	56281.0
96-97	183165.0
98-99	409506.0
100-101	3979723.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.75094419789028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.30415388759559	77.43666460852904
2	6.827324481017538	11.708990411852502
3	1.5931319203595233	4.098376992078818
4	0.5718083887722243	1.961324369499702
5	0.27229425352425285	1.16747446696822
6	0.14203624455502395	0.7307845248549453
7	0.08097101350601615	0.48603386025607087
8	0.05768127506764985	0.39569790396713617
9	0.036080816641109764	0.2784567684965503
>10	0.11139596187546084	1.452798757012854
>50	0.0023661492335714383	0.13952677619499915
>100	7.2340908780289E-4	0.11951788374149573
>500	3.21987642741431E-5	0.024352676547685262
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	3.520299985883597E-5	0.0	0.0	0.0
5	0.0	3.520299985883597E-5	0.0	0.0	0.0
6	5.280449978825395E-5	3.520299985883597E-5	0.0	0.0	0.0
7	5.280449978825395E-5	5.280449978825395E-5	0.0	0.0	0.0
8	5.280449978825395E-5	8.800749964708992E-5	0.0	0.0	0.0
9	5.280449978825395E-5	1.7601499929417985E-4	0.0	1.7601499929417985E-5	0.0
10-11	6.160524975296294E-5	1.7601499929417985E-4	0.0	2.640224989412698E-5	0.0
12-13	7.040599971767194E-5	1.7601499929417985E-4	0.0	3.520299985883597E-5	0.0
14-15	7.920674968238094E-5	2.464209990118518E-4	0.0	3.520299985883597E-5	0.0
16-17	1.056089995765079E-4	2.552217489765608E-4	0.0	7.040599971767194E-5	0.0
18-19	1.056089995765079E-4	2.8162399887068777E-4	0.0	8.800749964708992E-5	0.0
20-21	1.056089995765079E-4	2.9922549880010573E-4	0.0	8.800749964708992E-5	0.0
22-23	1.056089995765079E-4	3.1682699872952376E-4	0.0	1.056089995765079E-4	0.0
24-25	1.056089995765079E-4	3.344284986589417E-4	0.0	1.232104995059259E-4	0.0
26-27	1.056089995765079E-4	3.344284986589417E-4	0.0	1.9361649922359784E-4	0.0
28-29	1.056089995765079E-4	3.344284986589417E-4	0.0	3.520299985883597E-4	0.0
30-31	1.056089995765079E-4	3.696314985177777E-4	0.0	8.536727465767723E-4	0.0
32-33	1.056089995765079E-4	3.696314985177777E-4	0.0	0.0020153717419183594	0.0
34-35	1.056089995765079E-4	4.752404980942856E-4	0.0	0.003432292486236507	3.520299985883597E-5
36-37	1.056089995765079E-4	5.808494976707935E-4	0.0	0.005368457478472486	3.520299985883597E-5
38-39	1.056089995765079E-4	5.984509976002115E-4	0.0	0.008703941715097193	3.520299985883597E-5
40-41	1.056089995765079E-4	7.040599971767194E-4	0.0	0.012593873199498569	3.520299985883597E-5
42-43	1.056089995765079E-4	7.216614971061375E-4	0.0	0.01616697768517042	3.520299985883597E-5
44-45	1.144097495412169E-4	7.832667468591003E-4	0.0	0.020549751167595498	3.520299985883597E-5
46-47	1.232104995059259E-4	8.096689967532273E-4	0.0	0.025117340399279466	3.520299985883597E-5
48-49	1.232104995059259E-4	8.184697467179362E-4	0.0	0.029596922131316344	4.400374982354496E-5
50-51	1.232104995059259E-4	9.592817461532802E-4	0.0	0.03424371811268269	5.280449978825395E-5
52-53	1.232104995059259E-4	9.680824961179892E-4	0.0	0.039154536592990306	5.280449978825395E-5
54-55	1.232104995059259E-4	9.680824961179892E-4	0.0	0.04401255057350967	5.280449978825395E-5
56-57	1.232104995059259E-4	9.680824961179892E-4	0.0	0.04784967755812279	5.280449978825395E-5
58-59	1.232104995059259E-4	9.680824961179892E-4	0.0	0.05220604879065374	5.280449978825395E-5
60-61	1.3201124947063487E-4	0.0010120862459415342	0.0	0.056386405023890515	5.280449978825395E-5
62-63	1.9361649922359784E-4	0.0010296877458709523	0.0	0.0611652122547275	5.280449978825395E-5
64-65	1.9361649922359784E-4	0.0012321049950592589	0.0	0.06554798573715258	5.280449978825395E-5
66-67	2.0241724918830683E-4	0.0012321049950592589	0.0	0.06984275171993057	5.280449978825395E-5
68-69	2.112179991530158E-4	0.001240905745023968	0.0	0.07402310795316733	5.280449978825395E-5
70-71	2.464209990118518E-4	0.001267307994918095	0.0	0.07825626868619237	7.040599971767194E-5
72-73	2.8162399887068777E-4	0.0012849094948475129	0.0	0.08165335817257002	8.800749964708992E-5
74-75	2.8162399887068777E-4	0.0013201124947063488	0.0	0.08447839891124162	8.800749964708992E-5
76-77	2.8162399887068777E-4	0.001346514744600476	0.0	0.08701301490107782	8.800749964708992E-5
78-79	2.8162399887068777E-4	0.0013553154945651848	0.0	0.08854434539493718	8.800749964708992E-5
80-81	2.8162399887068777E-4	0.0013553154945651848	0.0	0.08910759339267854	8.800749964708992E-5
82-83	2.8162399887068777E-4	0.0013553154945651848	0.0	0.08976764964003173	8.800749964708992E-5
84-85	2.8162399887068777E-4	0.0013553154945651848	0.0	0.09001407063904357	8.800749964708992E-5
86-87	2.8162399887068777E-4	0.0013729169944946028	0.0	0.09010207813869067	8.800749964708992E-5
88	2.8162399887068777E-4	0.0014609244941416928	0.0	0.09010207813869067	8.800749964708992E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7035	0.0	48.728867	1
GTATCAA	12895	0.0	38.46779	1
TCAACGC	16160	0.0	31.424955	4
ATCAACG	16240	0.0	31.243196	3
TATCAAC	16805	0.0	30.55963	2
CAACGCA	16630	0.0	30.484169	5
AACGCAG	17145	0.0	29.568487	6
ACGCAGA	19690	0.0	25.791128	7
CGCAGAG	19900	0.0	25.496962	8
CTTATAC	3680	0.0	25.340805	1
GTGGTAT	3435	0.0	25.108925	1
TGGTATC	3385	0.0	24.574436	2
GCAGAGT	23430	0.0	21.674234	9
TACATGG	16205	0.0	20.154799	2
TATACAC	5940	0.0	20.12029	3
GTACATG	16390	0.0	19.927301	1
GAGTACT	14580	0.0	19.802284	12-13
ACATGGG	16260	0.0	19.681349	3
AGAGTAC	21175	0.0	19.496035	10-11
CAGAGTA	22715	0.0	18.87773	10-11
>>END_MODULE