FastQCFastQC Report
Fri 27 May 2016
SRR2140126_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140126_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5508270
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64750.049.2983671
CTTATAC83000.040.361031
GTATCAA119450.038.0124741
TATACAC109800.032.9944273
TCAACGC141100.031.6024384
TTATACA110150.031.4200632
ATCAACG143550.031.0930773
CAACGCA144700.030.7572175
TATCAAC148700.030.334352
AACGCAG149500.029.7696956
ACGCAGA172200.025.8453527
CGCAGAG174700.025.4754988
TACACAT139750.025.404045
ACACATC136050.025.216736
ACATCTC141250.024.4394448
CACATCT150150.022.8487267
ATACACA165250.022.6458914
GTGGTAT33350.022.5209921
TGGTATC30600.021.8951992
GCAGAGT209650.021.289639
Produced by FastQC (version 0.11.5)