##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140126_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5508270
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.574097130314964	33.0	32.0	33.0	28.0	34.0
2	32.40210937372351	33.0	33.0	34.0	31.0	34.0
3	32.56852550800887	33.0	33.0	34.0	31.0	34.0
4	32.96057636971317	33.0	33.0	34.0	31.0	34.0
5	33.10202822301739	34.0	33.0	34.0	33.0	34.0
6	36.55081032701737	38.0	37.0	38.0	34.0	38.0
7	37.10046312181502	38.0	38.0	38.0	36.0	38.0
8	37.033779208353984	38.0	38.0	38.0	36.0	38.0
9	37.20563189531377	38.0	38.0	38.0	36.0	38.0
10-11	37.32105270801904	38.0	38.0	38.0	37.0	38.0
12-13	37.36236595156011	38.0	38.0	38.0	37.0	38.0
14-15	37.38440390540042	38.0	38.0	38.0	37.0	38.0
16-17	37.39016760253219	38.0	38.0	38.0	37.0	38.0
18-19	37.41222098771483	38.0	38.0	38.0	37.0	38.0
20-21	37.42520964380063	38.0	38.0	38.0	37.0	38.0
22-23	37.44035918123936	38.0	38.0	38.0	37.0	38.0
24-25	37.44923407868652	38.0	38.0	38.0	37.0	38.0
26-27	37.41936706724251	38.0	38.0	38.0	37.0	38.0
28-29	37.392609850376076	38.0	38.0	38.0	37.0	38.0
30-31	37.38646643608952	38.0	38.0	38.0	37.0	38.0
32-33	37.372373119659564	38.0	38.0	38.0	37.0	38.0
34-35	37.338464589818685	38.0	38.0	38.0	37.0	38.0
36-37	37.31573137519023	38.0	38.0	38.0	37.0	38.0
38-39	37.25934458518162	38.0	38.0	38.0	37.0	38.0
40-41	37.31185074569892	38.0	38.0	38.0	37.0	38.0
42-43	37.28411980203916	38.0	38.0	38.0	37.0	38.0
44-45	37.240260727728014	38.0	38.0	38.0	37.0	38.0
46-47	37.2086305042945	38.0	38.0	38.0	37.0	38.0
48-49	37.161694538691506	38.0	38.0	38.0	36.5	38.0
50-51	37.12473118025041	38.0	38.0	38.0	36.0	38.0
52-53	37.0833191095203	38.0	38.0	38.0	36.0	38.0
54-55	37.05553764890356	38.0	38.0	38.0	36.0	38.0
56-57	37.00808812831575	38.0	38.0	38.0	36.0	38.0
58-59	36.96889515144534	38.0	38.0	38.0	36.0	38.0
60-61	36.98120615155639	38.0	38.0	38.0	36.0	38.0
62-63	36.98876780048411	38.0	38.0	38.0	36.0	38.0
64-65	36.99214063922416	38.0	38.0	38.0	36.0	38.0
66-67	36.983718749719294	38.0	38.0	38.0	36.0	38.0
68-69	36.933535911760785	38.0	38.0	38.0	36.0	38.0
70-71	36.925748342296615	38.0	38.0	38.0	35.5	38.0
72-73	36.929947228718554	38.0	38.0	38.0	36.0	38.0
74-75	36.93831070461262	38.0	38.0	38.0	36.0	38.0
76-77	36.91544358525639	38.0	38.0	38.0	35.0	38.0
78-79	36.87228157026908	38.0	38.0	38.0	35.0	38.0
80-81	36.82248728041583	38.0	38.0	38.0	35.0	38.0
82-83	36.76934692612542	38.0	38.0	38.0	35.0	38.0
84-85	36.740018737536076	38.0	38.0	38.0	35.0	38.0
86-87	36.75685590809354	38.0	38.0	38.0	35.0	38.0
88-89	36.709995571162025	38.0	38.0	38.0	34.5	38.0
90-91	36.71730402379996	38.0	38.0	38.0	34.0	38.0
92-93	36.69996475115875	38.0	38.0	38.0	34.0	38.0
94-95	36.706929814521736	38.0	38.0	38.0	34.0	38.0
96-97	36.697059506131694	38.0	38.0	38.0	34.0	38.0
98-99	36.67894308499204	38.0	38.0	38.0	34.0	38.0
100	35.11595796284899	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	8.0
19	9.0
20	23.0
21	86.0
22	301.0
23	978.0
24	2385.0
25	4864.0
26	8896.0
27	14726.0
28	22567.0
29	32888.0
30	44733.0
31	58877.0
32	77122.0
33	103243.0
34	153360.0
35	268651.0
36	709126.0
37	4005426.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.616618284869844	19.680425977666307	14.178117630399381	25.52483810706447
2	14.145400261595121	19.87770682318799	43.18932166104912	22.787571254167766
3	18.141506806689158	25.249162257831788	30.36535325687359	26.243977678605468
4	12.497142897601995	17.83578056219061	38.18784456564146	31.479231974565934
5	13.586951983109033	36.70716940164516	35.71150288566102	13.994375729584787
6	30.179330352361085	37.244307196270334	18.44165227920926	14.134710172159318
7	26.53462883990799	32.24063090589242	22.39225746014629	18.832482794053305
8	24.14447730412634	36.83207250189261	21.080702289466565	17.942747904514484
9	24.96923716520795	17.158926486900604	20.92373830621956	36.948098041671884
10-11	24.219900259065007	26.458189231827777	28.62058323212188	20.70132727698533
12-13	25.069413808691294	23.661149507921724	28.771783155146718	22.497653528240264
14-15	22.218573163624878	25.226895195769274	26.077525248399226	26.477006392206626
16-17	22.118024352473643	28.73320479932901	26.72752969625672	22.421241151940627
18-19	22.11871422424827	27.409821958618586	28.490987188354964	21.980476628778185
20-21	22.638768752377704	26.59539597452998	28.439073988124797	22.326761284967517
22-23	22.368427860916007	26.96481868948513	28.341680743929437	22.32507270566942
24-25	22.421724548738986	27.11781132768162	28.424412265159578	22.03605185841982
26-27	22.300259275780107	27.277279272164755	28.403912751797893	22.01854870025724
28-29	22.027344922859903	26.938138433127058	28.48747941329316	22.54703723071988
30-31	22.755584160224934	26.950380140602253	28.459279480497994	21.83475621867482
32-33	22.522695513074222	27.04157595915705	28.573159297583878	21.862569230184846
34-35	22.429245537197616	27.123875999606177	28.682456999512834	21.764421463683373
36-37	22.39777687885057	26.900644256973706	28.58168296043543	22.1198959037403
38-39	21.94876722105374	26.685609326316893	28.959613684081436	22.406009768547932
40-41	22.201566790469375	26.537339625333683	28.923725421834984	22.337368162361958
42-43	22.357724601798594	26.52647878675351	28.87975250551203	22.23604410593587
44-45	22.03674081451429	26.902775589018603	28.761912857254078	22.298570739213034
46-47	22.5170263057799	26.536690698227382	28.184949727886284	22.761333268106437
48-49	22.311815520308755	26.6146867074584	28.33237873074051	22.74111904149233
50-51	21.99902140955551	26.872411454902007	28.55609750459437	22.572469630948117
52-53	22.21991130539619	26.938506115413137	28.682126797194357	22.159455781996314
54-55	22.123098415898422	26.90546366546857	28.3261843625369	22.645253556096105
56-57	22.41624206188632	27.25844716752531	27.884540138964425	22.440770631623945
58-59	21.893302194804626	27.614095434756052	28.482411813382413	22.010190557056905
60-61	22.22187160793156	28.029791785040597	27.773215353843604	21.975121253184238
62-63	21.765573420572483	28.447620497198294	27.73933458757914	22.047471494650082
64-65	21.77769882567097	28.287931242290398	27.8067626409019	22.12760729113673
66-67	21.814486433297713	28.193516718270484	27.67852238515888	22.313474463272925
68-69	21.85940102858043	27.971679034878544	27.887909786387898	22.281010150153133
70-71	22.008434006816007	27.482827164929173	27.99889327988394	22.509845548370873
72-73	22.21154456293435	27.16358841160546	28.093756981996513	22.531110043463677
74-75	22.08041895024643	27.26908159878111	28.048243407769025	22.602256043203436
76-77	22.325026714887347	27.205839889571227	27.83458958073801	22.63454381480341
78-79	22.37082826771613	27.270292131227176	27.737177976564336	22.621701624492356
80-81	22.402473590307014	27.29574874615064	27.686491429319705	22.615286234222644
82-83	22.4174601554314	27.267225364360048	27.78038619518294	22.534928285025615
84-85	22.50101741755763	27.267027153218354	27.746024635250738	22.48593079397328
86-87	22.364033817899543	27.289685886197812	27.810206092673557	22.536074203229084
88-89	22.449022686651713	27.323355653277837	27.737134969292764	22.490486690777683
90-91	22.511992429811283	27.313728176536355	27.727531623781836	22.44674776987053
92-93	22.563305703768304	27.328542210573602	27.72866102273764	22.379491062920454
94-95	22.45912469971775	27.434297580894	27.705367977086738	22.40120974230151
96-97	22.530692934663456	27.505208641798372	27.805431595573697	22.158666827964474
98-99	23.51119664328104	29.422203048231392	28.071523894791966	18.9950764136956
100	30.089571853244923	40.44585928611391	29.464568860641165	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1139.0
1	1004.0
2	1113.0
3	1529.5
4	1951.0
5	2634.5
6	3327.5
7	4312.5
8	5556.5
9	6282.0
10	6184.0
11	5976.0
12	6725.0
13	8445.5
14	11520.5
15	15207.0
16	18042.5
17	19656.5
18	19772.0
19	18659.5
20	17048.5
21	15722.5
22	15870.5
23	18216.5
24	22934.0
25	29829.5
26	39162.5
27	49990.5
28	60211.0
29	71309.0
30	82574.5
31	94150.5
32	107493.5
33	120070.5
34	132277.0
35	144318.0
36	157261.0
37	169423.0
38	179191.0
39	187751.0
40	197600.0
41	207590.5
42	214595.5
43	220427.5
44	234730.5
45	256519.0
46	258231.0
47	251313.5
48	246256.5
49	234732.5
50	227010.0
51	220039.0
52	211290.5
53	200975.0
54	188910.5
55	176501.0
56	162786.5
57	147703.0
58	131818.0
59	113320.5
60	94589.5
61	76774.0
62	60114.5
63	46073.0
64	34288.5
65	25535.0
66	19109.0
67	14459.0
68	10958.0
69	8052.0
70	5886.0
71	4252.5
72	2983.5
73	1973.5
74	1293.0
75	849.0
76	565.0
77	365.5
78	237.0
79	159.5
80	106.0
81	66.0
82	38.0
83	23.5
84	21.5
85	20.0
86	15.5
87	13.5
88	9.0
89	5.0
90	4.5
91	3.5
92	2.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.009476659640867278
3	2.1785424461763858E-4
4	0.0012163528657818154
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	9.077711748519788E-6
24-25	4.539139364651216E-5
26-27	6.355425731357425E-4
28-29	0.0011261598765583464
30-31	8.363159663535543E-4
32-33	5.738690634839464E-4
34-35	0.0011051052850244625
36-37	0.0
38-39	2.3974102068448643E-4
40-41	6.865167734602264E-4
42-43	2.893812394329173E-4
44-45	0.0
46-47	0.0
48-49	0.0016345231164104498
50-51	0.0034865884810436495
52-53	0.0
54-55	0.003298740476472807
56-57	0.03775678693477589
58-59	0.054377642828195856
60-61	0.02903042184557213
62-63	0.00606368948913819
64-65	0.0
66-67	0.0
68-69	1.0386762528175392E-5
70-71	0.003166465843301389
72-73	0.007464680066478308
74-75	0.005153760674417463
76-77	0.006610439218639572
78-79	0.010045318958047373
80-81	0.010715796399868402
82-83	0.04145069159468614
84-85	0.024544946934365413
86-87	9.661903840640877E-4
88-89	0.001377141426227337
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	45.0
20-21	145.0
22-23	345.0
24-25	428.0
26-27	1252.0
28-29	3220.0
30-31	10709.0
32-33	13460.0
34-35	18552.0
36-37	29515.0
38-39	32916.0
40-41	32778.0
42-43	36879.0
44-45	42955.0
46-47	43825.0
48-49	40591.0
50-51	35815.0
52-53	32924.0
54-55	35614.0
56-57	36653.0
58-59	39699.0
60-61	45045.0
62-63	43643.0
64-65	48245.0
66-67	54837.0
68-69	56421.0
70-71	71836.0
72-73	42915.0
74-75	40274.0
76-77	41557.0
78-79	42983.0
80-81	43113.0
82-83	40382.0
84-85	39747.0
86-87	41493.0
88-89	42849.0
90-91	43363.0
92-93	43436.0
94-95	51585.0
96-97	171628.0
98-99	1299571.0
100-101	2715027.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.49548431738638
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.19154272173924	78.0116116904908
2	7.097898569469889	12.278723488039642
3	1.6201761249404774	4.204137558185787
4	0.5286564772787037	1.829055921589794
5	0.23332191699778487	1.0090646106292214
6	0.11344792498076033	0.588763992960803
7	0.07084493526068984	0.4289436890765077
8	0.03746906654217245	0.2592724045988467
9	0.027934082464485636	0.21745547925547457
>10	0.07659356741512978	0.9914982627896615
>50	0.0017373995364914942	0.0964617498348257
>100	3.4619546347209646E-4	0.06483567531362423
>500	3.101791067996508E-5	0.02017547723496312
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	5.4463561154409646E-5	0.0	0.0	0.0
5	0.0	9.077260192401607E-5	0.0	0.0	0.0
6	0.0	9.077260192401607E-5	0.0	0.0	1.8154520384803214E-5
7	0.0	9.077260192401607E-5	0.0	0.0	1.8154520384803214E-5
8	0.0	1.0892712230881929E-4	0.0	0.0	3.630904076960643E-5
9	0.0	1.8154520384803215E-4	0.0	5.4463561154409646E-5	3.630904076960643E-5
10-11	0.0	1.8154520384803215E-4	0.0	7.261808153921286E-5	3.630904076960643E-5
12-13	0.0	1.8154520384803215E-4	0.0	7.261808153921286E-5	4.538630096200804E-5
14-15	0.0	2.813950659644498E-4	0.0	1.452361630784257E-4	6.354082134681125E-5
16-17	0.0	2.904723261568514E-4	0.0	1.6339068346322893E-4	9.077260192401607E-5
18-19	0.0	3.1770410673405625E-4	0.0	1.8154520384803215E-4	1.361589028860241E-4
20-21	0.0	3.812449280808675E-4	0.0	1.9969972423283536E-4	1.9969972423283536E-4
22-23	0.0	3.903221882732691E-4	0.0	2.0877698442523697E-4	2.1785424461763858E-4
24-25	0.0	3.9939944846567073E-4	0.0	2.1785424461763858E-4	2.1785424461763858E-4
26-27	0.0	3.9939944846567073E-4	0.0	2.632405455796466E-4	2.1785424461763858E-4
28-29	0.0	3.9939944846567073E-4	0.0	5.990991726985061E-4	2.2693150481004016E-4
30-31	0.0	4.901720503896868E-4	0.0	0.0018880701200195342	2.450860251948434E-4
32-33	0.0	5.0832657077449E-4	0.0	0.004466012014661591	2.54163285387245E-4
34-35	0.0	6.263309532757109E-4	0.0	0.008478161019703102	2.54163285387245E-4
36-37	0.0	7.443353357769318E-4	0.0	0.01485947493496143	2.54163285387245E-4
38-39	0.0	7.806443765465382E-4	0.0	0.033658480793425156	2.723178057720482E-4
40-41	0.0	9.077260192401607E-4	0.0	0.054136779787483186	2.723178057720482E-4
42-43	1.8154520384803214E-5	9.168032794325623E-4	0.0	0.0655559731095244	2.813950659644498E-4
44-45	1.8154520384803214E-5	0.0010075758813565784	0.0	0.07922632695928122	3.1770410673405625E-4
46-47	1.8154520384803214E-5	0.001107425743472996	0.0	0.09394964299135664	3.2678136692645786E-4
48-49	1.8154520384803214E-5	0.0011255802638577993	0.0	0.10738398807611102	3.2678136692645786E-4
50-51	1.8154520384803214E-5	0.001225430125974217	0.0	0.12020107946778208	3.2678136692645786E-4
52-53	1.8154520384803214E-5	0.0012345073861666186	0.0	0.1627189662089912	3.2678136692645786E-4
54-55	1.8154520384803214E-5	0.0012435846463590202	0.0	0.21677405065474276	3.2678136692645786E-4
56-57	1.8154520384803214E-5	0.0012526619065514218	0.0	0.29260548230206584	3.449358873112611E-4
58-59	1.8154520384803214E-5	0.0012889709473210282	0.0	0.3406604977606399	3.903221882732691E-4
60-61	1.8154520384803214E-5	0.0013071254677058314	0.0	0.35807976006985864	4.0847670865807234E-4
62-63	1.8154520384803214E-5	0.001316202727898233	0.0	0.37284846240289604	4.5386300962008033E-4
64-65	1.8154520384803214E-5	0.0014523616307842572	0.0	0.38030089302085773	4.5386300962008033E-4
66-67	1.8154520384803214E-5	0.0014614388909766588	0.0	0.3867639022778477	4.5386300962008033E-4
68-69	1.8154520384803214E-5	0.0014886706715538636	0.0	0.39279120304560233	4.5386300962008033E-4
70-71	1.8154520384803214E-5	0.00152497971232347	0.0	0.397529532866036	4.5386300962008033E-4
72-73	1.8154520384803214E-5	0.0015431342327082733	0.0	0.4010878188614574	4.7201753000488355E-4
74-75	1.8154520384803214E-5	0.001597597793862683	0.0	0.4039017695211019	4.7201753000488355E-4
76-77	1.8154520384803214E-5	0.0016792931355942974	0.0	0.40678833826228566	4.7201753000488355E-4
78-79	1.8154520384803214E-5	0.0017065249161715022	0.0	0.40854024947941914	4.7201753000488355E-4
80-81	1.8154520384803214E-5	0.0017609884773259117	0.0	0.40901226700942406	4.8109479019728515E-4
82-83	1.8154520384803214E-5	0.0017791429977107149	0.0	0.4095478253607757	4.901720503896868E-4
84-85	1.8154520384803214E-5	0.0017882202579031165	0.0	0.40992907028885656	4.901720503896868E-4
86-87	1.8154520384803214E-5	0.0018517610792499277	0.0	0.4100470746713578	4.901720503896868E-4
88	1.8154520384803214E-5	0.0019969972423283535	0.0	0.4100561519315502	5.0832657077449E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6475	0.0	49.298367	1
CTTATAC	8300	0.0	40.36103	1
GTATCAA	11945	0.0	38.012474	1
TATACAC	10980	0.0	32.994427	3
TCAACGC	14110	0.0	31.602438	4
TTATACA	11015	0.0	31.420063	2
ATCAACG	14355	0.0	31.093077	3
CAACGCA	14470	0.0	30.757217	5
TATCAAC	14870	0.0	30.33435	2
AACGCAG	14950	0.0	29.769695	6
ACGCAGA	17220	0.0	25.845352	7
CGCAGAG	17470	0.0	25.475498	8
TACACAT	13975	0.0	25.40404	5
ACACATC	13605	0.0	25.21673	6
ACATCTC	14125	0.0	24.439444	8
CACATCT	15015	0.0	22.848726	7
ATACACA	16525	0.0	22.645891	4
GTGGTAT	3335	0.0	22.520992	1
TGGTATC	3060	0.0	21.895199	2
GCAGAGT	20965	0.0	21.28963	9
>>END_MODULE