FastQCFastQC Report
Fri 27 May 2016
SRR2140125_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140125_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5614892
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62200.048.5210571
CTTATAC77750.043.2404171
GTATCAA119450.038.078781
TATACAC103950.034.1990663
TTATACA106500.032.6170962
TCAACGC137200.032.5219464
ATCAACG137950.032.2828063
CAACGCA139350.031.9887525
AACGCAG143700.031.0802366
TATCAAC147300.030.4998072
ACGCAGA165850.026.8515767
CGCAGAG167800.026.5651538
TACACAT139250.025.5291185
ACACATC133650.025.1514686
CACATCT145500.023.0144187
GCAGAGT201600.022.1325959
ATACACA170550.022.0541114
GTGGTAT31850.021.192091
CATCTCC159000.021.0603629
TGGTATC31250.020.7735372
Produced by FastQC (version 0.11.5)