FastQCFastQC Report
Fri 27 May 2016
SRR2140124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5822815
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC76350.052.650891
GGTATCA67500.048.992081
GTATCAA131100.038.8199161
TATACAC110850.038.2746933
TTATACA107300.038.1445162
TCAACGC150950.033.11394
ATCAACG151100.033.1094473
CAACGCA156100.032.0480965
TATCAAC158500.031.6484572
AACGCAG160500.031.1695216
ACACATC141600.028.3851956
TACACAT150350.027.9328145
ACATCTC145100.027.8780738
ACGCAGA184150.027.0965297
CGCAGAG185750.026.8168938
CACATCT159450.025.1806167
CATCTCC163550.024.47069
ATACACA197800.023.0779614
GCAGAGT222150.022.4808469
GTGGTAT35850.022.1626421
Produced by FastQC (version 0.11.5)