##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140124_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5822815
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.650431621131702	33.0	32.0	33.0	28.0	34.0
2	32.44422362723184	33.0	33.0	34.0	31.0	34.0
3	32.615845428714465	33.0	33.0	34.0	31.0	34.0
4	32.98979565725512	33.0	33.0	34.0	31.0	34.0
5	33.14273319691593	34.0	33.0	34.0	33.0	34.0
6	36.61678552384027	38.0	37.0	38.0	34.0	38.0
7	37.14398534042383	38.0	38.0	38.0	36.0	38.0
8	37.095477874533195	38.0	38.0	38.0	36.0	38.0
9	37.24472338551027	38.0	38.0	38.0	36.0	38.0
10-11	37.349607277579665	38.0	38.0	38.0	37.0	38.0
12-13	37.381619199648284	38.0	38.0	38.0	37.0	38.0
14-15	37.40180204935242	38.0	38.0	38.0	37.0	38.0
16-17	37.4114295233491	38.0	38.0	38.0	37.0	38.0
18-19	37.42343153612128	38.0	38.0	38.0	37.0	38.0
20-21	37.43327878876718	38.0	38.0	38.0	37.0	38.0
22-23	37.44626736834029	38.0	38.0	38.0	37.0	38.0
24-25	37.4531942415816	38.0	38.0	38.0	37.0	38.0
26-27	37.42075265729035	38.0	38.0	38.0	37.0	38.0
28-29	37.40227290401283	38.0	38.0	38.0	37.0	38.0
30-31	37.39835954537328	38.0	38.0	38.0	37.0	38.0
32-33	37.38556587947197	38.0	38.0	38.0	37.0	38.0
34-35	37.350637486811266	38.0	38.0	38.0	37.0	38.0
36-37	37.32327839648623	38.0	38.0	38.0	37.0	38.0
38-39	37.26942343799686	38.0	38.0	38.0	37.0	38.0
40-41	37.32074202501836	38.0	38.0	38.0	37.0	38.0
42-43	37.29080852994514	38.0	38.0	38.0	37.0	38.0
44-45	37.24729793265836	38.0	38.0	38.0	37.0	38.0
46-47	37.21571616486467	38.0	38.0	38.0	37.0	38.0
48-49	37.17371442679945	38.0	38.0	38.0	37.0	38.0
50-51	37.14206425091825	38.0	38.0	38.0	36.5	38.0
52-53	37.10525107099624	38.0	38.0	38.0	36.0	38.0
54-55	37.08506985110575	38.0	38.0	38.0	36.0	38.0
56-57	37.039013908441774	38.0	38.0	38.0	36.0	38.0
58-59	37.003686878273854	38.0	38.0	38.0	36.0	38.0
60-61	37.013488839495636	38.0	38.0	38.0	36.0	38.0
62-63	37.02292656063999	38.0	38.0	38.0	36.0	38.0
64-65	37.02751834252237	38.0	38.0	38.0	36.0	38.0
66-67	37.01200564738596	38.0	38.0	38.0	36.0	38.0
68-69	36.96406003328954	38.0	38.0	38.0	36.0	38.0
70-71	36.95728415894432	38.0	38.0	38.0	36.0	38.0
72-73	36.96147277234246	38.0	38.0	38.0	36.0	38.0
74-75	36.97664700656304	38.0	38.0	38.0	36.0	38.0
76-77	36.955784304146	38.0	38.0	38.0	36.0	38.0
78-79	36.915608076559664	38.0	38.0	38.0	36.0	38.0
80-81	36.86858431799085	38.0	38.0	38.0	35.0	38.0
82-83	36.827351419658044	38.0	38.0	38.0	35.0	38.0
84-85	36.79841430205529	38.0	38.0	38.0	35.0	38.0
86-87	36.818828791702124	38.0	38.0	38.0	35.0	38.0
88-89	36.77204780984766	38.0	38.0	38.0	35.0	38.0
90-91	36.7846440223464	38.0	38.0	38.0	35.0	38.0
92-93	36.77320094916992	38.0	38.0	38.0	35.0	38.0
94-95	36.78887034570913	38.0	38.0	38.0	35.0	38.0
96-97	36.7842916768474	38.0	38.0	38.0	35.0	38.0
98-99	36.76347674959616	38.0	38.0	38.0	35.0	38.0
100	35.16268830513727	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	9.0
19	8.0
20	39.0
21	86.0
22	357.0
23	1052.0
24	2526.0
25	5287.0
26	9392.0
27	15831.0
28	24180.0
29	35349.0
30	47720.0
31	62809.0
32	80951.0
33	108119.0
34	156586.0
35	269542.0
36	699668.0
37	4303299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.47341363240975	19.988167235263358	14.323845081803215	25.214574050523673
2	13.923831698330464	20.14454300186939	43.66869499083854	22.26293030896161
3	17.988973014041683	25.67243353118754	30.501926390815697	25.836667063955076
4	12.058964003023318	18.421107317363873	38.85501307889638	30.66491560071643
5	13.305780795027836	37.0936565904979	35.96106007146028	13.63950254301399
6	29.487730590788132	37.8897320282372	18.86013895340999	13.762398427564673
7	26.226438586834718	32.47369184835857	22.620897280782575	18.678972284024137
8	23.642928720902177	37.2480836159143	21.375880909834848	17.733106753348682
9	24.742637366978	17.400260870386575	21.095707145083605	36.761394617551815
10-11	23.79061502039821	26.85537836939693	29.01194697066625	20.342059639538608
12-13	24.84450390403954	24.01448440316239	29.056873694252694	22.084137998545376
14-15	21.879434603366242	25.59133340145617	26.437968577054228	26.09126341812337
16-17	21.742602160638796	29.07134264097348	27.090865844097745	22.095189354289978
18-19	21.719280107645528	27.79669283671214	28.919002235173192	21.565024820469137
20-21	22.246201014024674	26.976132190732265	28.851564332793266	21.926102462449794
22-23	21.91165533094794	27.382375119245957	28.723700518563728	21.982269031242378
24-25	21.94501864000529	27.508405392848523	28.95889065131382	21.587685315832367
26-27	22.04413740244149	27.597518573381528	28.934030773998188	21.4243132501788
28-29	21.671213862423244	27.220301926008606	29.27922966528882	21.82925454627933
30-31	22.236824182083858	27.385115769979056	28.90682401697336	21.471236030963723
32-33	21.936174529864527	27.053068131248924	29.1058928642971	21.904864474589445
34-35	21.588933480929303	27.55754411640734	29.497487897268783	21.35603450539458
36-37	22.001648968129285	27.376598505214115	28.93825506369402	21.683497462962585
38-39	21.543137553601017	27.113765051074566	29.352719694880765	21.99037770044366
40-41	21.819691106661075	27.010438594530488	29.297734206569075	21.872136092239362
42-43	22.07482432987189	26.944565335462585	29.19823744422359	21.782372890441938
44-45	21.716443004255943	27.41922197153852	29.022337124835513	21.841997899370025
46-47	22.182106272275007	26.990479147216462	28.472570343950483	22.35484423655805
48-49	21.998277956006458	27.026027859097525	28.589032100007312	22.38666208488871
50-51	21.68157619261525	27.268820305897005	28.83867541142499	22.21092809006275
52-53	21.847170609393636	27.315862832062678	29.08934721883097	21.74761933971272
54-55	21.85031960674998	27.29498150497609	28.626085778553357	22.228613109720577
56-57	22.215537422224298	27.6806627464626	28.09116138711176	22.012638444201343
58-59	21.598927894316848	28.122784758456298	28.774766920043515	21.503520427183336
60-61	22.029874415908186	28.666369238417467	27.86367988993022	21.440076455744126
62-63	21.527466827827734	29.070849322111204	27.87162937099791	21.53005447906315
64-65	21.519038015648352	28.99427432123649	27.857393451813838	21.629294211301318
66-67	21.620642532194868	28.838640495610633	27.810006275689446	21.730710696505053
68-69	21.67797986318015	28.494054742381277	28.06332918426152	21.76463621017706
70-71	21.758483980657036	28.051328115076373	28.23170783503049	21.958480069236103
72-73	21.973150470539565	27.650381834077088	28.364881517100137	22.011586178283217
74-75	21.892827987525916	27.742328391258937	28.26803430827194	22.096809312943208
76-77	21.97350554747827	27.688286454195676	28.21400896772267	22.124199030603386
78-79	21.96852326934127	27.727788645327582	28.17494002091031	22.128748064420837
80-81	22.015416984484553	27.732318359249014	28.12315282105801	22.12911183520842
82-83	22.073204630687883	27.744411082972707	28.207613581604523	21.97477070473489
84-85	22.17929630181132	27.703959142143397	28.12844554929019	21.988299006755092
86-87	21.96885417034924	27.71474983403874	28.22605807800036	22.09033791761166
88-89	22.086782949024084	27.69634428556863	28.142877277217586	22.0739954881897
90-91	22.11796317637412	27.728886948794734	28.135546582560057	22.01760329227109
92-93	22.09684372567431	27.724684267846982	28.15059105966622	22.027880946812484
94-95	21.999595778695998	27.855242513507545	28.102004750426385	22.043156957370073
96-97	22.103836016967524	27.9465673214743	28.177712048219156	21.77188461333902
98-99	23.175364625701416	29.82380744117552	28.44855603544923	18.55227189767383
100	22.1129643667516	30.765330949278656	22.32930154107134	24.792403142898394
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	952.0
1	864.5
2	1084.5
3	1520.0
4	1969.0
5	2817.0
6	3633.5
7	4696.5
8	6039.0
9	6768.0
10	6638.0
11	6530.0
12	7332.5
13	9272.5
14	13004.5
15	17432.5
16	20867.0
17	22729.5
18	22463.0
19	20848.0
20	19248.5
21	18007.0
22	18292.5
23	21091.0
24	26332.5
25	33817.5
26	43893.0
27	55491.5
28	66858.5
29	79144.0
30	92010.0
31	105579.0
32	120220.0
33	134889.5
34	149296.0
35	162771.0
36	177111.0
37	190065.0
38	199113.5
39	207945.5
40	218361.0
41	227261.5
42	236112.5
43	257383.0
44	277700.5
45	280537.5
46	270563.5
47	258130.5
48	249174.0
49	240117.5
50	232556.0
51	223222.0
52	211232.5
53	198296.5
54	183897.0
55	168026.5
56	152472.0
57	136399.0
58	119510.0
59	103198.5
60	86375.5
61	69869.5
62	54863.0
63	42301.5
64	32089.0
65	23857.5
66	17394.5
67	13065.5
68	10002.5
69	7301.0
70	5333.0
71	3895.0
72	2725.0
73	1895.5
74	1303.0
75	848.0
76	531.5
77	329.0
78	216.5
79	139.0
80	89.5
81	52.0
82	36.0
83	26.0
84	19.0
85	14.0
86	10.0
87	8.0
88	7.5
89	6.5
90	5.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.010355815872563356
3	1.545644160084083E-4
4	0.0013052106240710034
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	8.587339685102254E-6
24-25	1.0305451876030223E-4
26-27	7.643926985896525E-4
28-29	0.0012801174477688455
30-31	7.567507325003113E-4
32-33	7.064959806840552E-4
34-35	0.0011573988100903754
36-37	0.0
38-39	2.3530184826115854E-4
40-41	7.713727445784111E-4
42-43	3.4386224843059945E-4
44-45	0.0
46-47	0.0
48-49	0.0019476084315215015
50-51	0.003914244444111445
52-53	0.0
54-55	0.0037914419792505053
56-57	0.04014830405389437
58-59	0.05637760579553199
60-61	0.03066170787217717
62-63	0.006757767950001236
64-65	0.0
66-67	0.0
68-69	4.8781977439896456E-5
70-71	0.003437191158991228
72-73	0.00791879064950001
74-75	0.0055618082437909125
76-77	0.0069912328718610825
78-79	0.009934797758102043
80-81	0.010880638054695135
82-83	0.04230982844158137
84-85	0.02475180550787157
86-87	9.873177443292928E-4
88-89	0.0014565069878064662
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	45.0
20-21	168.0
22-23	357.0
24-25	439.0
26-27	1359.0
28-29	3559.0
30-31	10619.0
32-33	13302.0
34-35	18526.0
36-37	28589.0
38-39	33562.0
40-41	32924.0
42-43	36299.0
44-45	43216.0
46-47	44127.0
48-49	40910.0
50-51	36496.0
52-53	35680.0
54-55	37881.0
56-57	39262.0
58-59	39147.0
60-61	40883.0
62-63	41211.0
64-65	48474.0
66-67	56035.0
68-69	57673.0
70-71	76263.0
72-73	42596.0
74-75	39714.0
76-77	41141.0
78-79	42251.0
80-81	41591.0
82-83	39568.0
84-85	39135.0
86-87	40744.0
88-89	41940.0
90-91	42965.0
92-93	42805.0
94-95	51646.0
96-97	175122.0
98-99	397388.0
100-101	3927203.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.93596862912189
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.13983326876087	80.14469519185154
2	6.557017594243922	11.531953869360676
3	1.3892870497067122	3.6650490725956426
4	0.46909429164348004	1.6500104365624486
5	0.19087520382353734	0.8392397967751912
6	0.09239709035859331	0.4875016583517241
7	0.051008050140930614	0.3139809607917885
8	0.031956748148350506	0.22481180821296792
9	0.01838129529591055	0.1454739305853434
>10	0.05842803462014071	0.7827494459716444
>50	0.0011303495005430407	0.07298773777455034
>100	5.359106572315552E-4	0.09749209887427221
>500	2.755655027832463E-5	0.017010947824427053
>1k	2.755654947173515E-5	0.02704304446778656
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.7173824000934257E-5	0.0	0.0	0.0	0.0
6	1.7173824000934257E-5	0.0	0.0	0.0	0.0
7	1.7173824000934257E-5	0.0	0.0	1.7173824000934257E-5	0.0
8	1.7173824000934257E-5	0.0	0.0	1.7173824000934257E-5	0.0
9	1.7173824000934257E-5	5.1521472002802766E-5	0.0	6.869529600373703E-5	0.0
10-11	1.7173824000934257E-5	5.1521472002802766E-5	0.0	6.869529600373703E-5	0.0
12-13	1.7173824000934257E-5	5.1521472002802766E-5	0.0	6.869529600373703E-5	0.0
14-15	1.7173824000934257E-5	5.1521472002802766E-5	0.0	7.728220800420416E-5	0.0
16-17	1.7173824000934257E-5	5.1521472002802766E-5	0.0	1.0304294400560553E-4	0.0
18-19	1.7173824000934257E-5	5.1521472002802766E-5	0.0	1.202167680065398E-4	1.7173824000934257E-5
20-21	1.7173824000934257E-5	5.1521472002802766E-5	0.0	1.545644160084083E-4	1.7173824000934257E-5
22-23	1.7173824000934257E-5	8.586912000467128E-5	0.0	1.803251520098097E-4	3.4347648001868515E-5
24-25	1.7173824000934257E-5	8.586912000467128E-5	0.0	1.8891206401027683E-4	7.728220800420416E-5
26-27	1.7173824000934257E-5	8.586912000467128E-5	0.0	2.490204480135467E-4	8.586912000467128E-5
28-29	1.7173824000934257E-5	8.586912000467128E-5	0.0	5.495623680298962E-4	8.586912000467128E-5
30-31	1.7173824000934257E-5	1.0304294400560553E-4	0.0	0.0013223844480719376	8.586912000467128E-5
32-33	1.7173824000934257E-5	1.0304294400560553E-4	0.0	0.0031943312641737713	8.586912000467128E-5
34-35	1.7173824000934257E-5	1.288036800070069E-4	0.0	0.005426928384295225	1.202167680065398E-4
36-37	1.7173824000934257E-5	1.545644160084083E-4	0.0	0.008896040832483944	1.202167680065398E-4
38-39	1.7173824000934257E-5	1.545644160084083E-4	0.0	0.014056774944764688	1.202167680065398E-4
40-41	1.7173824000934257E-5	1.8891206401027683E-4	0.0	0.01908011846503796	1.202167680065398E-4
42-43	1.7173824000934257E-5	1.9749897601074396E-4	0.0	0.024172157281314964	1.2880368000700693E-4
44-45	1.7173824000934257E-5	2.3184662401261246E-4	0.0	0.029787997729620465	1.3739059200747406E-4
46-47	1.7173824000934257E-5	2.5760736001401386E-4	0.0	0.03538666435392503	1.4597750400794116E-4
48-49	1.7173824000934257E-5	2.5760736001401386E-4	0.0	0.0409853309782296	1.8891206401027683E-4
50-51	1.7173824000934257E-5	2.9195500801588233E-4	0.0	0.04644660701052669	1.8891206401027683E-4
52-53	1.7173824000934257E-5	3.091288320168166E-4	0.0	0.0515644065628051	2.1467280001167822E-4
54-55	1.7173824000934257E-5	3.091288320168166E-4	0.0	0.05684535744309239	2.404335360130796E-4
56-57	1.7173824000934257E-5	3.091288320168166E-4	0.0	0.06217782979538247	2.404335360130796E-4
58-59	1.7173824000934257E-5	3.091288320168166E-4	0.0	0.06775073568368564	2.404335360130796E-4
60-61	1.7173824000934257E-5	3.091288320168166E-4	0.0	0.07304886038797385	2.404335360130796E-4
62-63	1.7173824000934257E-5	3.091288320168166E-4	0.0	0.07835557200426255	2.404335360130796E-4
64-65	1.7173824000934257E-5	3.2630265601775086E-4	0.0	0.08351630611654329	2.404335360130796E-4
66-67	1.7173824000934257E-5	3.2630265601775086E-4	0.0	0.08910638582884739	2.490204480135467E-4
68-69	1.7173824000934257E-5	3.3488956801821796E-4	0.0	0.09405244714111646	2.5760736001401386E-4
70-71	1.7173824000934257E-5	3.434764800186851E-4	0.0	0.09836307696535096	2.5760736001401386E-4
72-73	1.7173824000934257E-5	3.434764800186851E-4	0.0	0.10159175587752658	2.9195500801588233E-4
74-75	1.7173824000934257E-5	3.434764800186851E-4	0.0	0.10419359021366814	2.9195500801588233E-4
76-77	1.7173824000934257E-5	3.520633920191523E-4	0.0	0.10689846749381528	3.091288320168166E-4
78-79	1.7173824000934257E-5	3.606503040196194E-4	0.0	0.10884769651792131	3.2630265601775086E-4
80-81	1.7173824000934257E-5	3.606503040196194E-4	0.0	0.10926845520594419	3.3488956801821796E-4
82-83	1.7173824000934257E-5	3.606503040196194E-4	0.0	0.1096119316859629	3.7782412802055365E-4
84-85	1.7173824000934257E-5	3.606503040196194E-4	0.0	0.10983519139797504	3.7782412802055365E-4
86-87	1.7173824000934257E-5	3.606503040196194E-4	0.0	0.11001551654998484	3.864110400210208E-4
88	1.7173824000934257E-5	3.949979520214879E-4	0.0	0.11010138566998952	3.949979520214879E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	7635	0.0	52.65089	1
GGTATCA	6750	0.0	48.99208	1
GTATCAA	13110	0.0	38.819916	1
TATACAC	11085	0.0	38.274693	3
TTATACA	10730	0.0	38.144516	2
TCAACGC	15095	0.0	33.1139	4
ATCAACG	15110	0.0	33.109447	3
CAACGCA	15610	0.0	32.048096	5
TATCAAC	15850	0.0	31.648457	2
AACGCAG	16050	0.0	31.169521	6
ACACATC	14160	0.0	28.385195	6
TACACAT	15035	0.0	27.932814	5
ACATCTC	14510	0.0	27.878073	8
ACGCAGA	18415	0.0	27.096529	7
CGCAGAG	18575	0.0	26.816893	8
CACATCT	15945	0.0	25.180616	7
CATCTCC	16355	0.0	24.4706	9
ATACACA	19780	0.0	23.077961	4
GCAGAGT	22215	0.0	22.480846	9
GTGGTAT	3585	0.0	22.162642	1
>>END_MODULE