FastQCFastQC Report
Fri 27 May 2016
SRR2140123_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140123_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5787683
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76850.050.4912381
CTTATAC57250.043.6821
GTATCAA147550.039.3751141
TATACAC74050.036.0552523
TTATACA77900.032.9717332
ATCAACG179250.031.8432253
TCAACGC180500.031.6461474
TATCAAC183900.031.1779882
CAACGCA184200.031.0334455
AACGCAG191350.029.9401846
TACACAT100700.026.6813965
ACGCAGA218950.026.1467157
CGCAGAG222350.025.7659288
ACACATC106100.023.7681396
ACATCTC107200.023.6821298
ATACACA119600.023.667874
GTGGTAT37850.023.588871
GCAGAGT258500.022.1790549
TGGTATC37350.022.0919742
CACATCT120750.020.709267
Produced by FastQC (version 0.11.5)